VARIAÇÃO SAZONAL DA COMPOSIÇÃO CORPORAL, ESTILO DE VIDA, PRESSÃO ARTERIAL E FREQÜÊNCIA CARDÍACA DE MOTORISTAS DE ÔNIBUS QUE FAZEM O TRANSPORTE DOS ACADÊMICOS DA UNIVERSIDADE DO OESTE DE SANTA CATARINA: ORIENTAÇÕES PARA A PROMOÇÃO DA SAÚDE

O estilo de vida compreende as escolhas e os hábitos de vida de uma pessoa e pode variar também em razão das estações do ano (variabilidade sazonal). Motoristas de ônibus possuem jornadas de trabalho diferenciadas das demais profissões e isso pode acarretar em mudanças no estilo de vida e na saúde. Objetivou-se analisar a variação sazonal da composição corporal (%G e massa magra), dos indicadores de gordura (circunferência de cintura – CC e índice de massa corporal – IMC), estilo de vida, pressão arterial e frequência cardíaca de motoristas de ônibus que fazem o transporte dos acadêmicos da Universidade do Oeste de Santa Catarina, e realizar orientações para a promoção da saúde. A amostra foi composta, na primeira avaliação, por 23 motoristas, na segunda, por 13, na terceira, por 11, na quarta, por 7, na quinta e na sexta, por 5 e 7, respectivamente. Os motoristas foram avaliados uma vez por mês durante um período de seis meses. Foram realizadas medidas de peso, estatura, circunferência de cintura, dobras cutâneas, estilo de vida, pressão arterial e freqüência cardíaca. A partir dos resultados, foi proposta a cada mês orientações para a promoção da saúde por meio de palestras, cartilhas educativas, caminhada e musculação.  Para a análise dos dados, utilizou-se o programa estatístico computacional SPSS versão 17.0. Foi usada a estatística descritiva (média e desvio padrão) para caracterizar a amostra e ANOVA – Oneway com teste pos hoc de Tukey para determinar as diferenças entre os seis momentos de avaliações. Para estabelecer as correlações entre as variáveis, foi utilizada a correlação de Spearman. Os resultados mostraram que na comparação do % de gordura, do IMC, da CC, da pressão arterial (sistólica e diastólica), de repouso e da freqüência cardíaca de repouso durante as seis avaliações, não houve diferenças estatisticamente significativas (P≤0,05). Sobre os componentes do estilo de vida, perceberam-se diferenças estatisticamente significativas (P≤0,05) somente para a prática de atividade física. A análise de correlação apontou que à medida que aumenta a massa corporal dos motoristas, a PAS, a PAD, a FC e a ingesta de alimentos não saudáveis também aumentam, e a prática de atividade física diminui. Ainda, isso reflete em uma CC elevada, o que provoca maiores valores de PAS e PAD e diminuição da prática de atividade física. Esse fato também foi percebido para as variáveis % de gordura e IMC, pois à medida que aumentam, influenciam o aumento da PAD e a diminuição da prática de atividade física dos motoristas. Conclui-se que, embora tenha existido uma desistência acentuada na amostra deste estudo da primeira para a última avaliação, é importante considerar que as orientações oferecidas contribuíram para uma vida mais saudável dos motoristas que participaram até o final da pesquisa.Palavras-chave: Motoristas de ônibus. Estilo de vida. Saúde

The iron-sulfur helicase DDX11 promotes the generation of single-stranded DNA for CHK1 activation

The iron-sulfur (FeS) cluster helicase DDX11 is associated with a human disorder termed Warsaw Breakage Syndrome. Interestingly, one disease-associated mutation affects the highly conserved arginine-263 in the FeS cluster-binding motif. Here, we demonstrate that the FeS cluster in DDX11 is required for DNA binding, ATP hydrolysis, and DNA helicase activity, and that arginine-263 affects FeS cluster binding, most likely because of its positive charge. We further show that DDX11 interacts with the replication factors DNA polymerase delta and WDHD1. In vitro, DDX11 can remove DNA obstacles ahead of Pol δ in an ATPase- and FeS domain-dependent manner, and hence generate single-stranded DNA. Accordingly, depletion of DDX11 causes reduced levels of single-stranded DNA, a reduction of chromatin-bound replication protein A, and impaired CHK1 phosphorylation at serine-345. Taken together, we propose that DDX11 plays a role in dismantling secondary structures during DNA replication, thereby promoting CHK1 activation

Investigation of the distribution of Strauzia longipennis Wied. in Berlin and in the Federal Land Brandenburg

Außerhalb Nordamerikas wurde die dort endemische Sonnenblumenfruchtfliege Strauzia longipennis Wied. erstmals 2009/10 durch Sichtungen und Fänge in Berlin dokumentiert. Da die Adaption dieser Fruchtfliege an gemäßigte Klimate den Verdacht nahelegte, dass eine Überwinterung in unseren Breiten möglich ist und darüber hinaus die Art als Quarantäneschädling geführt wird, fanden im Jahr 2011 umfangreiche systematische Erhebungen zur Verbreitung dieser Art in Berlin und im Bundesland Brandenburg an Sonnenblumen Helianthus annuus L. und Topinambur H. tuberosus L. statt. Es wurden Pflanzen sowohl von Feldern mit erwerbsmäßigem Anbau von H. annuus als auch von Feldern mit Durchwuchs, von Feldrändern, aus einer Gärtnerei, von Versuchsflächen und aus dem Bereich von Haus- und Kleingärten auf den Befall mit S. longi­pennis untersucht. Dieses Monitoring wurde 2012 als Übersichtsbonitur weitergeführt. Im Jahr 2011 wurden im Land Brandenburg insgesamt 27 Fundorte festgestellt, wobei es sich hauptsächlich um Schläge mit feldmäßigem Anbau von H. annuus handelte. Nur in den Landkreisen Oberspreewald-Lausitz und Prignitz konnte der Schädling nicht beobachtet werden. In Berlin wurden 16 Fundorte nach­gewiesen. Im Jahr 2012 sank die Zahl der in Brandenburg positiv bonitierten Orte auf zwei; hier war nur ein geringer Befall zu verzeichnen. An zwei Standorten in Berlin wurde S. longipennis jedoch mit hoher Abundanz nachgewiesen. Die Ergebnisse der beiden Boniturjahre belegen, dass die Sonnenblumenfruchtfliege in Berlin und im Land Brandenburg territorial bereits weit verbreitet ist, aber deutlichen Abundanzschwankungen unterliegt. Das wiederholte Auftreten der Art auf Flächen in Berlin und im Land Brandenburg im Jahr 2012 beweist, dass sich die Art in der Region dauerhaft etabliert hat. Zusätzlich belegen die Ergebnisse, dass die Einschleppung in Deutschland bereits deutlich früher als 2009/10 – bei der ersten Registrierung in Berlin – stattgefunden haben muss. Darüber hinaus zeigten die Untersuchungen, dass Topinambur ebenfalls zum Wirtspflanzenspektrum der Fruchtfliege in Deutschland gehört. Das Auftreten der Art in Berlin und im Land Brandenburg über mehrere Jahre und der Mangel an Wissen über die eingeschleppte Population macht weitere Forschung zu verschiedenen Aspekten dringend notwendig.    The sunflower maggot Strauzia longipennis Wied. is endemic in North America. First observations on the fruit fly in Europe were obtained in Berlin and dates back to 2009/10. Considering the adaption of the species to climatic conditions in Central Europe and the status as quarantine pest an extensive survey on the occurrence of the fruit fly was carried out in Berlin and Federal Land Brandenburg in 2011. The infestation with S. longipennis was investigated on sunflower H. annuus as well as Jerusalem artichoke H. tuberosus L. Therefore, plants were examined growing on fields i.e. sunflower fields and other crops (plants growing through or growing on the edges) as well as plants from a nursery, from experimental fields, backyards, allotments and roadsides. Further monitoring was conducted in 2012 in Land Brandenburg to achieve an overview of the situation of infestation, whereas in Berlin, two sides were closely examined. In 2011 S. longipennis was found in Land Brandenburg and Berlin. In Brandenburg, on 27 locations mostly cultivated with Helianthus annuus L. were plants registered to be infested with the fruit fly. Only in districts Oberspreewald-Lausitz and Prigniz, the sunflower maggot could not be detected. In Berlin infested plants were observed at 16 locations. One year later, the number of positively tested fields in Brandenburg declined. The sunflower maggot could be detected only on two fields. Furthermore, abundance on these fields was low. Nevertheless, relatively high abundance of the species was observed at two locations in Berlin. The results of both years verify the territorial wide distribution of the sunflower maggot within Berlin and the Federal Land Brandenburg. Further, significant variations of abundance between the years were shown. Despite the differences in the years, the repeated presence of S. longipennis in Berlin and Land Brandenburg proves the permanent establishment of the species within the region. Additionally, it can be assumed that the introduction of the species in Germany must have taken place quite earlier than with the first detection in Berlin in 2009/10. Furthermore, the study demonstrates the Jerusalem artichoke H. tuberosus as another host plant of the fly species within Germany. The occurence of S. longipennis in Berlin and Land Brandenburg for several years and the lack of knowledge regarding the introduced population emphasize the need for further research.   &nbsp

The Ursinus Weekly, May 22, 1975

Dr. Cope accepts history post at U. of Nebraska • Questionnaire summarized • UC faculty hears speaker from AAUP • U.C. Plans for Bicentennial • From the cluttered desk of the U.S.G.A. president • George Bause wins Scotland scholarship • Editorial: Last moments • Focus: Ms. Swanson • Wolsey Hall: British study • Alvarez attends seminar • Changes at library: Dr. Yost resigns post • Letters to the editor: Pets • Alumni elects • Intramural golf winners • Senior comments: Geoffery Higgins • Commencement • Earns degree • From the president • Cub and Key elects • Senior Spotlight: Dave McNamara • Reflections • Spring festival review • Study center success • New professors appointed • Ursinus bear squad • Volunteers needed at Pennhurst • Mulch queen contest • Concert review: Jesse Colin Young • Miller chosen • Dick Allen returns • Lacrosse wins 2 • Women\u27s tennis given team honors • Baseball: Season ends wrap-up • New results • Golf wins seven • Faculty-student net tournament • Intramural winnershttps://digitalcommons.ursinus.edu/weekly/1039/thumbnail.jp

The Ursinus Weekly, May 22, 1975

Dr. Cope accepts history post at U. of Nebraska • Questionnaire summarized • UC faculty hears speaker from AAUP • U.C. Plans for Bicentennial • From the cluttered desk of the U.S.G.A. president • George Bause wins Scotland scholarship • Editorial: Last moments • Focus: Ms. Swanson • Wolsey Hall: British study • Alvarez attends seminar • Changes at library: Dr. Yost resigns post • Letters to the editor: Pets • Alumni elects • Intramural golf winners • Senior comments: Geoffery Higgins • Commencement • Earns degree • From the president • Cub and Key elects • Senior Spotlight: Dave McNamara • Reflections • Spring festival review • Study center success • New professors appointed • Ursinus bear squad • Volunteers needed at Pennhurst • Mulch queen contest • Concert review: Jesse Colin Young • Miller chosen • Dick Allen returns • Lacrosse wins 2 • Women\u27s tennis given team honors • Baseball: Season ends wrap-up • New results • Golf wins seven • Faculty-student net tournament • Intramural winnershttps://digitalcommons.ursinus.edu/weekly/1039/thumbnail.jp

Expression of the Serpin Serine Protease Inhibitor 6 Protects Dendritic Cells from Cytotoxic T Lymphocyte–Induced Apoptosis: Differential Modulation by T Helper Type 1 and Type 2 Cells

Dendritic cells (DCs) play a central role in the immune system as they drive activation of T lymphocytes by cognate interactions. However, as DCs express high levels of major histocompatibility complex class I, this intimate contact may also result in elimination of DCs by activated cytotoxic T lymphocytes (CTLs) and thereby limit induction of immunity. We show here that immature DCs are indeed susceptible to CTL-induced killing, but become resistant upon maturation with anti-CD40 or lipopolysaccharide. Protection is achieved by expression of serine protease inhibitor (SPI)-6, a member of the serpin family that specifically inactivates granzyme B and thereby blocks CTL-induced apoptosis. Anti-CD40 and LPS-induced SPI-6 expression is sustained for long periods of time, suggesting a role for SPI-6 in the longevity of DCs. Importantly, T helper 1 cells, which mature DCs and boost CTL immunity, induce SPI-6 expression and subsequent DC resistance. In contrast, T helper 2 cells neither induce SPI-6 nor convey protection, despite the fact that they trigger DC maturation with comparable efficiency. Our data identify SPI-6 as a novel marker for DC function, which protects DCs against CTL-induced apoptosis

Functional Locomotor Consequences of Uneven Forefeet for Trot Symmetry in Individual Riding Horses

ABSTRACT: Left-right symmetrical distal limb conformation can be an important prerequisite for a successful performance, and it is often hypothesized that asymmetric or uneven feet are important enhancing factors for the development of lameness. On a population level, it has been demonstrated that uneven footed horses are retiring earlier from elite level competition, but the biomechanical consequences are not yet known. The objectives of this study were to compare the functional locomotor asymmetries of horses with uneven to those with even feet. Hoof kinetics and distal limb kinematics were collected from horses (n = 34) at trot. Dorsal hoof wall angle was used to classify horses as even or uneven (1.5° difference between forefeet respectively) and individual feet as flat (55°). Functional kinetic parameters were compared between even and uneven forefeet using MANOVA followed by ANOVA. The relative influences of differences in hoof angle between the forefeet and of absolute hoof angle on functional parameters were analysed using multiple regression analysis (P<0.05). In horses with uneven feet, the side with the flatter foot showed a significantly larger maximal horizontal braking and vertical ground reaction force, a larger vertical fetlock displacement and a suppler fetlock spring. The foot with a steeper hoof angle was linearly correlated with an earlier braking-propulsion transition. The conformational differences between both forefeet were more important for loading characteristics than the individual foot conformation of each individual horse. The differences in vertical force and braking force between uneven forefeet could imply either an asymmetrical loading pattern without a pathological component or a subclinical lameness as a result of a pathological development in the steeper foot

The Ursinus Weekly, March 14, 1974

College issues new Bulletin • Meistersingers begin on a good note; Plan singing tour through New England • The C.C.C. Rides Again! • Drs. Miller and Craft to present papers at Midwest convention • Ursinus volunteers see first fire of this year • Campus Chest to sponsor fast day this Thursday • Editorials: The seriousness of rock music; Streaking • Alumni corner: Dr. Margaret Brian • Letters to the editor: In search of a white knight; What an offer!; Administration admonished; Wanted: one clock; Thanks • Forums: Col. Frederick F. Woerner • Film: American graffiti • The Zodiac: The twelve zodiacal expressions of self • Ol’ blue eyes is back and better than ever! • The festival of the arts in words and pictures • Folk concert a big success • Philadelphia trio plays here • Film: Some reflections on Bergmann’s Seventh Seal • Streaking hits campus; The Ursinus Bares are here! • Hot item number 1 • The legends of the streakers • Hot item number 2 • Bearettes lose to bears in streaking • Travelin’ 7 plannedhttps://digitalcommons.ursinus.edu/weekly/1012/thumbnail.jp

Prospective study on the mismatch concept in acute stroke patients within the first 24 h after symptom onset - 1000Plus study

<p>Abstract</p> <p>Background</p> <p>The mismatch between diffusion weighted imaging (DWI) lesion and perfusion imaging (PI) deficit volumes has been used as a surrogate of ischemic penumbra. This pathophysiology-orientated patient selection criterion for acute stroke treatment may have the potential to replace a fixed time window. Two recent trials - DEFUSE and EPITHET - investigated the mismatch concept in a multicenter prospective approach. Both studies randomized highly selected patients (n = 74/n = 100) and therefore confirmation in a large consecutive cohort is desirable. We here present a single-center approach with a 3T MR tomograph next door to the stroke unit, serving as a bridge from the ER to the stroke unit to screen all TIA and stroke patients. Our primary hypothesis is that the prognostic value of the mismatch concept is depending on the vessel status. Primary endpoint of the study is infarct growth determined by imaging, secondary endpoints are neurological deficit on day 5-7 and functional outcome after 3 months.</p> <p>Methods and design</p> <p>1000Plus is a prospective, single centre observational study with 1200 patients to be recruited. All patients admitted to the ER with the clinical diagnosis of an acute cerebrovascular event within 24 hours after symptom onset are screened. Examinations are performed on day 1, 2 and 5-7 with neurological examination including National Institute of Health Stroke Scale (NIHSS) scoring and stroke MRI including T2*, DWI, TOF-MRA, FLAIR and PI. PI is conducted as dynamic susceptibility-enhanced contrast imaging with a fixed dosage of 5 ml 1 M Gadobutrol. For post-processing of PI, mean transit time (MTT) parametric images are determined by deconvolution of the arterial input function (AIF) which is automatically identified. Lesion volumes and mismatch are measured and calculated by using the perfusion mismatch analyzer (PMA) software from ASIST-Japan. Primary endpoint is the change of infarct size between baseline examination and day 5-7 follow up.</p> <p>Discussions</p> <p>The aim of this study is to describe the incidence of mismatch and the predictive value of PI for final lesion size and functional outcome depending on delay of imaging and vascular recanalization. It is crucial to standardize PI for future randomized clinical trials as for individual therapeutic decisions and we expect to contribute to this challenging task.</p> <p>Trial Registration</p> <p>clinicaltrials.gov NCT00715533</p

A horse’s locomotor signature: COP path determined by the individual limb

Introduction Ground reaction forces in sound horses with asymmetric hooves show systematic differences in the horizontal braking force and relative timing of break-over. The Center Of Pressure (COP) path quantifies the dynamic load distribution under the hoof in a moving horse. The objective was to test whether anatomical asymmetry, quantified by the difference in dorsal wall angle between the left and right forelimbs, correlates with asymmetry in the COP path between these limbs. In addition, repeatability of the COP path was investigated. Methods A larger group (n = 31) visually sound horses with various degree of dorsal hoof wall asymmetry trotted three times over a pressure mat. COP path was determined in a hoof-bound coordinate system. A relationship between correlations between left and right COP paths and degree of asymmetry was investigated. Results Using a hoof-bound coordinate system made the COP path highly repeatable and unique for each limb. The craniocaudal patterns are usually highly correlated between left and right, but the mediolateral patterns are not. Some patterns were found between COP path and dorsal wall angle but asymmetry in dorsal wall angle did not necessarily result in asymmetry in COP path and the same could be stated for symmetry. Conclusion This method is a highly sensitive method to quantify the net result of the interaction between all of the forces and torques that occur in the limb and its inertial properties. We argue that changes in motor control, muscle force, inertial properties, kinematics and kinetics can potentially be picked up at an early stage using this method and could therefore be used as an early detection method for changes in the musculoskeletal apparatus