3D-Printing for Analytical Ultracentrifugation

Analytical ultracentrifugation (AUC) is a classical technique of physical biochemistry providing information on size, shape, and interactions of macromolecules from the analysis of their migration in centrifugal fields while free in solution. A key mechanical element in AUC is the centerpiece, a component of the sample cell assembly that is mounted between the optical windows to allow imaging and to seal the sample solution column against high vacuum while exposed to gravitational forces in excess of 300,000 g. For sedimentation velocity it needs to be precisely sector-shaped to allow unimpeded radial macromolecular migration. During the history of AUC a great variety of centerpiece designs have been developed for different types of experiments. Here, we report that centerpieces can now be readily fabricated by 3D printing at low cost, from a variety of materials, and with customized designs. The new centerpieces can exhibit sufficient mechanical stability to withstand the gravitational forces at the highest rotor speeds and be sufficiently precise for sedimentation equilibrium and sedimentation velocity experiments. Sedimentation velocity experiments with bovine serum albumin as a reference molecule in 3D printed centerpieces with standard double-sector design result in sedimentation boundaries virtually indistinguishable from those in commercial double-sector epoxy centerpieces, with sedimentation coefficients well within the range of published values. The statistical error of the measurement is slightly above that obtained with commercial epoxy, but still below 1%. Facilitated by modern open-source design and fabrication paradigms, we believe 3D printed centerpieces and AUC accessories can spawn a variety of improvements in AUC experimental design, efficiency and resource allocation.Comment: 25 pages, 6 figure

Permafrost distribution offshore of West Yamal : extended abstract

The results of seismic studies in the near-shore, shallow waters of the south-western Kara Sea - at the Shpindler, Kharsavey and Mare-Sale sites - showed the presence of a seismic interface which can be interpreted as a submarine permafrost table. The proposed permafiost exhibits a continuous distribution and a strongly dissected top surface overlain by unfrozen sediments. The permafrost table is located at a depth of 4-6 m and 5-10 m below the sea floor at the Shpindler and Mare-Sale sites, respectively. Three dimensional modeling of the permafrost table suggests the presence of relict buried thermodenudational depressions (up to 2 km across) at a minimum sea depth of 40-45 m at the Shpindler and Mare-Sale sites. The depressions may be considered as paragenetic to thermocirques found in cliffs at the Shpindler site. At the Kharasavey site, the permafrost table has an elongated depression parallel to the modern shoreline. The maximum depression depth is 20 m below the seafloor. At present, the relict therrnocirques (Shpindler and Mare-Sale) and the elongated depression (Kharasavey) are completely filled in with sediment and are not evident in modern bottom topography

Rodent arena tracker (RAT): A machine vision rodent tracking camera and closed loop control system

Video tracking is an essential tool in rodent research. Here, we demonstrate a machine vision rodent tracking camera based on a low-cost, open-source, machine vision camera, the OpenMV Cam M7. We call our device the rodent arena tracker (RAT), and it is a pocket-sized machine vision-based position tracker. The RAT does not require a tethered computer to operate and costs about $120 per device to build. These features make the RAT scalable to large installations and accessible to research institutions and educational settings where budgets may be limited. The RAT processes incoming video in real-time at 15 Hz and save

Geological structure of the northern part of the Kara Shelf near the Severnaya Zemlya archipelago according to recent studies

Until recently, the North of the Kara Shelf was completely unexplored by seismic methods. Seismic and seismo-acoustic data that have appeared in recent years have made it possible to decipher features of the regional geological structure. This study solves the urgent problem of determining the prospects for the oil and gas potential of the North Kara sedimentary basin. The relevance of the research is associated with determining the prospects of the oil and gas potential of the North Kara sedimentary basin. The aim of the study is to clarify the age of the reflecting horizons using data on the geology of the island, as well as to determine the tectonic position of the sedimentary cover and basement structures in the north of the Kara shelf. The sedimentary cover is divided into three structural levels: Cambrian-Devonian, Middle Carboniferous-Cretaceous, Miocene-Quarter. The Cambrian-Devonian complex fills the deep troughs of the North Kara shelf. The most noticeable discontinuity is the base of Carboniferous-Permian rocks, lying on the eroded surface of folded Silurian-Devonian seismic complexes. The blanket-like plate part of the cover is composed of thin Carbon-Quarter complexes. The authors came to the conclusion that the fold structures of the Taimyr-Severozemelskiy fold belt gradually degenerate towards the Kara sedimentary basin and towards the continental slope of the Eurasian basin of the Arctic Ocean. A chain of narrow uplifts within the seabed relief, which correspond to narrow anticlines is traced to the West of the Severnaya Zemlya islands. Paleozoic rocks have subhorizontal bedding further to the West, within the Kara shelf. Mesozoic folding in the North of the Kara Sea is expressed exclusively in a weak activation of movements along faults. At the neotectonic stage, the shelf near Severnaya Zemlya was raised and the Mesozoic complexes were eroded. The modern seismic activity of the North Zemlya shelf is associated with the ongoing formation of the continental margin

Direct Measurement of Perchlorate Exposure Biomarkers in a Highly Exposed Population: A Pilot Study

Exposure to perchlorate is ubiquitous in the United States and has been found to be widespread in food and drinking water. People living in the lower Colorado River region may have perchlorate exposure because of perchlorate in ground water and locally-grown produce. Relatively high doses of perchlorate can inhibit iodine uptake and impair thyroid function, and thus could impair neurological development in utero. We examined human exposures to perchlorate in the Imperial Valley among individuals consuming locally grown produce and compared perchlorate exposure doses to state and federal reference doses. We collected 24-hour urine specimen from a convenience sample of 31 individuals and measured urinary excretion rates of perchlorate, thiocyanate, nitrate, and iodide. In addition, drinking water and local produce were also sampled for perchlorate. All but two of the water samples tested negative for perchlorate. Perchlorate levels in 79 produce samples ranged from non-detect to 1816 ppb. Estimated perchlorate doses ranged from 0.02 to 0.51 µg/kg of body weight/day. Perchlorate dose increased with the number of servings of dairy products consumed and with estimated perchlorate levels in produce consumed. The geometric mean perchlorate dose was 70% higher than for the NHANES reference population. Our sample of 31 Imperial Valley residents had higher perchlorate dose levels compared with national reference ranges. Although none of our exposure estimates exceeded the U. S. EPA reference dose, three participants exceeded the acceptable daily dose as defined by bench mark dose methods used by the California Office of Environmental Health Hazard Assessment

Chlorate and Other Oxychlorine Contaminants Within the Dairy Supply Chain

The presence of chlorate in milk and dairy products can arise from the use of chlorinated water and chlorinated detergents for cleaning and sanitation of process equipment at both farm and food processor level. Chlorate and other oxychlorine species have been associated with inhibition of iodine uptake in humans and the formation of methemoglobin, with infants and young children being a high‐risk demographic. This comprehensive review of chlorate and chlorine derivatives in dairy, highlights areas of concern relative to the origin and/or introduction of chlorate within the dairy supply chain. This review also discusses the associated health concerns, regulations, and chemical behavior of chlorate and chlorine‐derived by‐products, and provides a summary of mechanisms for their detection and removal