Demonstration of Metabolic and Cellular Effects of Portal Vein Ligation Using Multi-Modal PET/MRI Measurements in Healthy Rat Liver.

OBJECTIVES: In the early recognition of portal vein ligation (PVL) induced tumor progression, positron emission tomography and magnetic resonance imaging (PET/MRI) could improve diagnostic accuracy of conventionally used methods. It is unknown how PVL affects metabolic patterns of tumor free hepatic tissues. The aim of this preliminary study is to evaluate the effect of PVL on glucose metabolism, using PET/MRI imaging in healthy rat liver. MATERIALS AND METHODS: Male Wistar rats (n = 30) underwent PVL. 2-deoxy-2-(18F)fluoro-D-glucose (FDG) PET/MRI imaging (nanoScan PET/MRI) and morphological/histological examination were performed before (Day 0) and 1, 2, 3, and 7 days after PVL. Dynamic PET data were collected and the standardized uptake values (SUV) for ligated and non-ligated liver lobes were calculated in relation to cardiac left ventricle (SUVVOI/SUVCLV) and mean liver SUV (SUVVOI/SUVLiver). RESULTS: PVL induced atrophy of ligated lobes, while non-ligated liver tissue showed compensatory hypertrophy. Dynamic PET scan revealed altered FDG kinetics in both ligated and non-ligated liver lobes. SUVVOI/SUVCLV significantly increased in both groups of lobes, with a maximal value at the 2nd postoperative day and returned near to the baseline 7 days after the ligation. After PVL, ligated liver lobes showed significantly higher tracer uptake compared to the non-ligated lobes (significantly higher SUVVOI/SUVLiver values were observed at postoperative day 1, 2 and 3). The homogenous tracer biodistribution observed before PVL reappeared by 7th postoperative day. CONCLUSION: The observed alterations in FDG uptake dynamics should be taken into account during the assessment of PET data until the PVL induced atrophic and regenerative processes are completed

HiRISE - High-Resolution Imaging and Spectroscopy Explorer - ultrahigh resolution, interferometric and external occulting coronagraphic science

Recent solar physics missions have shown the definite role of waves and magnetic fields deep in the inner corona, at the chromosphere-corona interface, where dramatic and physically dominant changes occur. HiRISE (High Resolution Imaging and Spectroscopy Explorer), the ambitious new generation ultra-high resolution, interferometric, and coronagraphic, solar physics mission, proposed in response to the ESA Voyage 2050 Call, would address these issues and provide the best-ever and most complete solar observatory, capable of ultra-high spatial, spectral, and temporal resolution observations of the solar atmosphere, from the photosphere to the corona, and of new insights of the solar interior from the core to the photosphere. HiRISE, at the L1 Lagrangian point, would provide meter class FUV imaging and spectro-imaging, EUV and XUV imaging and spectroscopy, magnetic fields measurements, and ambitious and comprehensive coronagraphy by a remote external occulter (two satellites formation flying 375 m apart, with a coronagraph on a chaser satellite). This major and state-of-the-art payload would allow us to characterize temperatures, densities, and velocities in the solar upper chromosphere, transition zone, and inner corona with, in particular, 2D very high resolution multi-spectral imaging-spectroscopy, and, direct coronal magnetic field measurement, thus providing a unique set of tools to understand the structure and onset of coronal heating. HiRISE’s objectives are natural complements to the Parker Solar Probe and Solar Orbiter-type missions. We present the science case for HiRISE which will address: i) the fine structure of the chromosphere-corona interface by 2D spectroscopy in FUV at very high resolution; ii) coronal heating roots in the inner corona by ambitious externally-occulted coronagraphy; iii) resolved and global helioseismology thanks to continuity and stability of observing at the L1 Lagrange point; and iv) solar variability and space climate with, in addition, a global comprehensive view of UV variability

Statistical relationship between the succeeding solar flares detected by the RHESSI satellite

The Reuven Ramaty High Energy Solar Spectroscopic Imager (RHESSI) has observed more than 80,000 solar energetic events since its launch on February $12^{\rm th}$, 2002. Using this large sample of observed flares, we studied the spatio-temporal relationship between succeeding flares. Our results show that the statistical relationship between the temporal and spatial differences of succeeding flares can be described as a power law of the form $R(t)\sim t^p$ with $p=0.327\pm 0.007$. We discuss the possible interpretations of this result as a characteristic function of a supposed underlying physics. Different scenarios are considered to explain this relation, including the case where the connectivity between succeeding events is realised through a shock wave in the post Sedov-Taylor phase or where the spatial and temporal relationship between flares is supposed to be provided by an expanding flare area in the sub-diffusive regime. Furthermore, we cannot exclude the possibility that the physical process behind the statistical relationship is the reordering of the magnetic field by the flare or it is due to some unknown processes.Comment: 10 pages, 10 figure

Effect of Vitamin D Status on Vascular Function of the Aorta in a Rat Model of PCOS

Polycystic ovary syndrome (PCOS) is associated with elevated cardiovascular risk. Early vascular dysfunction may lead to the development of cardiovascular disease in PCOS. Vitamin D deficiency (VDD) is a common comorbidity of PCOS that contributes to the pathogenesis of the disease and its complications. Both PCOS and VDD are accompanied by increased oxidative stress that may be involved in the arising vascular dysfunction. We aimed to investigate the role of vitamin D status on aortic function. PCOS was induced by an 8-week-long transdermal testosterone treatment of female rats, and low and adequate vitamin D status was achieved by dietary means. Contraction and relaxation abilities of isolated aortic segments were measured by myograph. Resorcin-fuchsin staining and immunohistochemical labeling of 3-nitrotyrosine were performed. No difference was shown in the norepinephrine-induced contraction of the aortas of different groups, whereas we detected reduced acetylcholine- and insulin-evoked relaxation in VDD groups. A lower level of resorcin-fuchsin staining and elevated 3-nitrotyrosine immunostaining was observed in VDD. In our study, we demonstrated early endothelial dysfunction in VDD PCOS rat model. Vitamin D supplementation could prevent vascular disturbances, while VDD itself damaged endothelium-dependent vasorelaxation and induced nitrative stress

Accurate Quantitative Histomorphometric-Mathematical Image Analysis Methodology of Rodent Testicular Tissue and Its Possible Future Research Perspectives in Andrology and Reproductive Medicine

Infertility is increasing worldwide; male factors can be identified in nearly half of all infertile couples. Histopathologic evaluation of testicular tissue can provide valuable information about infertility; however, several different evaluation methods and semi-quantitative score systems exist. Our goal was to describe a new, accurate and easy-to-use quantitative computer-based histomorphometric-mathematical image analysis methodology for the analysis of testicular tissue. On digitized, original hematoxylin-eosin (HE)-stained slides (scanned by slide-scanner), quantitatively describable characteristics such as area, perimeter and diameter of testis cross-sections and of individual tubules were measured with the help of continuous magnification. Immunohistochemically (IHC)-stained slides were digitized with a microscope-coupled camera, and IHC-staining intensity measurements on digitized images were also taken. Suggested methods are presented with mathematical equations, step-by-step detailed characterization and representative images are given. Our novel quantitative histomorphometric-mathematical image analysis method can improve the reproducibility, objectivity, quality and comparability of andrological-reproductive medicine research by recognizing even the mild impairments of the testicular structure expressed numerically, which might not be detected with the present semi-quantitative score systems. The technique is apt to be subjected to further automation with machine learning and artificial intelligence and can be named ‘Computer-Assisted or -Aided Testis Histology’ (CATHI)

HiRISE - High-Resolution Imaging and Spectroscopy Explorer - Ultrahigh resolution, interferometric and external occulting coronagraphic science

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