Bumper 3 Update for IADC Protection Manual

The Bumper code has been the standard in use by NASA and contractors to perform meteoroid/debris risk assessments since 1990. It has undergone extensive revisions and updates [NASA JSC HITF website; Christiansen et al., 1992, 1997]. NASA Johnson Space Center (JSC) has applied BUMPER to risk assessments for Space Station, Shuttle, Mir, Extravehicular Mobility Units (EMU) space suits, and other spacecraft (e.g., LDEF, Iridium, TDRS, and Hubble Space Telescope). Bumper continues to be updated with changes in the ballistic limit equations describing failure threshold of various spacecraft components, as well as changes in the meteoroid and debris environment models. Significant efforts are expended to validate Bumper and benchmark it to other meteoroid/debris risk assessment codes. Bumper 3 is a refactored version of Bumper II. The structure of the code was extensively modified to improve maintenance, performance and flexibility. The architecture was changed to separate the frequently updated ballistic limit equations from the relatively stable common core functions of the program. These updates allow NASA to produce specific editions of the Bumper 3 that are tailored for specific customer requirements. The core consists of common code necessary to process the Micrometeoroid and Orbital Debris (MMOD) environment models, assess shadowing and calculate MMOD risk. The library of target response subroutines includes a board range of different types of MMOD shield ballistic limit equations as well as equations describing damage to various spacecraft subsystems or hardware (thermal protection materials, windows, radiators, solar arrays, cables, etc.). The core and library of ballistic response subroutines are maintained under configuration control. A change in the core will affect all editions of the code, whereas a change in one or more of the response subroutines will affect all editions of the code that contain the particular response subroutines which are modified. Note that the Bumper II program is no longer maintained or distributed by NASA

WG3 Document Status

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NASA WG3 MMOD Protection Summary

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NASA WG3 MMOD Protection Summary

No abstract availabl

Heavy-lift vehicle-launched Space Station method and apparatus

Methods and apparatus are provided for a single heavylift launch to place a complete, operational space station on-orbit. A payload including the space station takes the place of a Shuttle Orbiter using the launch vehicle of the Shuttle Orbiter. The payload includes a forward shroud, a core module, a propulsion module, and a transition module between the core module and the propulsion module. The essential subsystems are pre-integrated and verified on Earth. The core module provides means for attaching international modules with minimum impact to the overall design. The space station includes six control moment gyros for selectably operating in either LVLH (local-vertical local-horizontal) or SI (solar inertial) flight modes

Rapid genome editing by CRISPR-Cas9-POLD3 fusion

Precision CRISPR gene editing relies on the cellular homology-directed DNA repair (HDR) to introduce custom DNA sequences to target sites. The HDR editing efficiency varies between cell types and genomic sites, and the sources of this variation are incompletely understood. Here, we have studied the effect of 450 DNA repair protein-Cas9 fusions on CRISPR genome editing outcomes. We find the majority of fusions to improve precision genome editing only modestly in a locus- and cell-type specific manner. We identify Cas9-POLD3 fusion that enhances editing by speeding up the initiation of DNA repair. We conclude that while DNA repair protein fusions to Cas9 can improve HDR CRISPR editing, most need to be optimized to the cell type and genomic site, highlighting the diversity of factors contributing to locus-specific genome editing outcomes.Peer reviewe

Biohydrogen production through dark fermentation from waste biomass:Current status and future perspectives on biorefinery development

Green and clean hydrogen production has become a significant focus in recent years to achieve sustainable renewable energy fuel needs. Biohydrogen production through the dark fermentation (DF) process from organic wastes is advantageous with its environmentally friendly, energy-efficient, and cost-effective characteristics. This article elucidates the viability of transforming the DF process into a biorefinery system. Operational pH, temperature, feeding rate, inoculum-to-substrate ratio, and hydrogen partial pressure and its liquid-to-gas mass transfer rate are the factors that govern the performance of the DF process. Sufficient research has been made that can lead to upscaling the DF process into an industrial-scale technology. However, the DF process cannot be upscaled at the current technology readiness level as a stand-alone technology. Hence, it requires a downstream process (preferably anaerobic digestion) to improve energy recovery efficiency and economic viability. The article also discusses the possible hydrogen purification and storage techniques for achieving fuel quality and easy accessibility. The article further tries to unfold the opportunities, challenges, and current scenario/future research directions to enhance hydrogen yield and microbial metabolism, depicting the commercialization status for biorefinery development. Finally, the current progress gaps and policy-level loopholes from the Indian perspective are highlighted by analyzing the strengths, weaknesses, opportunities, and threats

The peak-flux of GRB 221009A measured with GRBAlpha

The brightest gamma-ray burst ever observed, long-duration GRB 221009A, was detected by GRBAlpha nano-satellite without saturation. We present light curves of the prompt emission in 13 energy bands, from 80 keV to 950 keV, and perform a spectral analysis to calculate the peak flux and peak isotropic-equivalent luminosity. Since the satellite's attitude information is not available for the time of this GRB, more than 200 incident directions were probed in order to find the median luminosity and its systematic uncertainty. We found that the peak flux in the $80-800$ keV range (observer frame) was $F_{\rm{ph}}^{\rm{p}}=1300_{-200}^{+1200}$ ph cm$^{-2}$s$^{-1}$ or $F_{\rm{erg}}^{\rm{p}}=5.7_{-0.7}^{+3.7}\times10^{-4}$ erg cm$^{-2}$s$^{-1}$ and the fluence in the same energy range of the first GRB episode lasting 300 s, which was observable by GRBAlpha, was $S=2.2_{-0.3}^{+1.4}\times10^{-2}$ erg cm$^{-2}$ or $S^{\rm{bol}}=4.9_{-0.5}^{+0.8}\times10^{-2}$ erg cm$^{-2}$ for the extrapolated range of $0.9-8,690$ keV. We infer the isotropic-equivalent released energy of the first GRB episode to be $E_{\rm{iso}}^{\rm{bol}}=2.8_{-0.5}^{+0.8}\times10^{54}$ erg in the $1-10,000$ keV band (rest frame at $z=0.15$). The peak isotropic-equivalent luminosity in the $92-920$ keV range (rest frame) was $L_{\rm{iso}}^{\rm{p}}=3.7_{-0.5}^{+2.5}\times10^{52}$ erg s$^{-1}$ and the bolometric peak isotropic-equivalent luminosity was $L_{\rm{iso}}^{\rm{p,bol}}=8.4_{-1.5}^{+2.5}\times10^{52}$ erg s$^{-1}$ (4 s scale) in the $1-10,000$ keV range (rest frame). The peak emitted energy is $E_p^\ast=E_p(1+z)=1120\pm470$ keV. Our measurement of $L_{\rm{iso}}^{\rm{p,bol}}$ is consistent with the Yonetoku relation. It is possible that, due to the spectral evolution of this GRB and orientation of GRBAlpha at the peak time, the true values of peak flux, fluence, $L_{\rm{iso}}$, and $E_{\rm{iso}}$ are even higher. [abridged]Comment: 7 pages, 7 figures, 1 table, accepted for publication in Astronomy & Astrophysic

Convergent antibody responses are associated with broad neutralization of hepatitis C virus

IntroductionEarly development of broadly neutralizing antibodies (bNAbs) targeting the hepatitis C virus (HCV) envelope glycoprotein E2 is associated with spontaneous clearance of infection, so induction of bNAbs is a major goal of HCV vaccine development. However, the molecular antibody features important for broad neutralization are not known.MethodsTo identify B cell repertoire features associated with broad neutralization, we performed RNA sequencing of the B cell receptors (BCRs) of HCV E2-reactive B cells of HCV-infected individuals with either high or low plasma neutralizing breadth. We then produced a monoclonal antibody (mAb) expressed by pairing the most abundant heavy and light chains from public clonotypes identified among clearance, high neutralization subjects.ResultsWe found distinctive BCR features associated with broad neutralization of HCV, including long heavy chain complementarity determining region 3 (CDRH3) regions, specific VH gene usage, increased frequencies of somatic hypermutation, and particular VH gene mutations. Most intriguing, we identified many E2-reactive public BCR clonotypes (heavy and light chain clones with the same V and J-genes and identical CDR3 sequences) present only in subjects who produced highly neutralizing plasma. The majority of these public clonotypes were shared by two subjects who cleared infection. A mAb expressing the most abundant public heavy and light chains from these clearance, high neutralization subjects had features enriched in high neutralization clonotypes, such as increased somatic hypermutation frequency and usage of IGHV1-69, and was cross-neutralizing.DiscussionTogether, these results demonstrate distinct BCR repertoires associated with high plasma neutralizing capacity. Further characterization of the molecular features and function of these antibodies can inform HCV vaccine development