Bacterial Carriage of Pathogenic Antibiotic-resistant Staphylococci Among Conditionally Healthy Infants and Preschool-age Children

Staphylococci are related to opportunistic microorganisms and are part of normal human microflora. However, there are varieties that can cause pyoinflammatory diseases in organisms with weakened immune status. In the event of an inadequate therapeutic approach, they are preserved in the body, and the person becomes a bacterial carrier and a source of dissemination of pathogenic microorganisms. The formation of antibiotic resistance also plays a significant role in the emergence of bacterial carriage. The situation is especially serious in organized children’s groups, where children are in close contact with one another. Thus, the study of the prevalence rate of antibiotic resistance among pathogenic strains of staphylococci in children’s groups is topical and worthy of attention. The purpose of this work is to identify carriers of pathogenic and antibiotic resistant staphylococci isolated from the microflora of the oral cavity of preschool children considered healthy. The results of the research can prove useful in practical healthcare and, particularly, in pediatric practice. Keywords: staphylococci, pathogenic cocci, bacterial carriage, antibiotic resistance, infant morbidity rat

Rhetorical Lacunes: Causes, Implications, Warning

The article analyzes the current state of rhetoric as an academic discipline and the prospects for its study. Rhetorical skills and skills of the student are one of the significant results of educational achieve­ments. However, in Russia, their formation is hampered by the absence of the majority of native speakers of ideas about the logical, stylistic and ethical laws of public speech. Purpose of article - based on the analysis of works devoted to modern rhetoric, to characterize the state of rhetorical education in modern society and the place of rhetorical (communication) disciplines in the system of school and university education. The causes of the decline of rhetoric, the ways of its development as a factor in the education of a rhetorical person are considered, the problem of speech influence and its study in a higher educational institution is touched upon.В статье рассматривается современное состояние риторики как учебной дисциплины и перспективы ее изучения

Peculiarities of schoolchildren's motivation for physical education and sport activities

In the article the study of the features of the motivation of schoolchildren for physical culture and sports activities is considered: the factors influencing the motivation of schoolchildren for physical training are analyzed, the results of the questioning of schoolchildren of 12-13 years, connected with the study of motivation to engage in physical culture and sports activities, are analyzedВ статье приводится исследование особенностей мотивации школьников к занятиям физкультурно-оздоровительной и спортивной деятельностью: рассматриваются факторы, влияющие на мотивацию школьников к занятиям физической культурой, анализируются результаты анкетирования школьников 11–12 лет, связанное с исследованием мотивации к занятиям физкультурно-оздоровительной и спортивной деятельность

THE TRANSFORMATION OF THE PRINCIPLES OF WORK OF THE PSYCHOLOGICAL SERVICE OF THE UNIVERSITY IN THE ASPECT OF DIGITALIZATION

The interference of non-digital and digital components of the educational environment creates the need to transform approaches to ensuring psychological well-being and academic success of students at the university. From the position of the ecopsychological approach, the types of interaction of students with the educational environment are characterized. Differences in the frequency of types of interaction depending on the level (bachelor’s and master’s degree) and the form of study (full-time and part-time) are revealed.Интерференция нецифрового и цифрового компонентов образовательной среды порождает необходимость трансформации подходов к обеспечению психологического благополучия и академической успешности обучающихся в вузе. С позиции экопсихологического подхода характеризуются типы взаимодействия обучающихся с образовательной средой. Выявлены различия в частоте типов взаимодействия в зависимости от уровня (бакалавриат и магистратура) и формы обучения (очная и заочная).Работа выполнена при финансовой поддержке гранта РФФИ 19-29-14067 МК

The influence of cross-country technological transfer on economic profit formation

The article covers the influence of transfer of technologies and export of ideas on development of economy and international competition. The surplus profit in innovative economy is provided at the expense of use of intangible assets. Innovations and technologies provide competitive advantage in the market. The countries, which produce an innovative product, have additional effect of economic growth because of fixing of a bigger value added as economic profit in the import prices. © IDOSI Publications, 2013

Haploid biotechnology as a tool for creating a selection material for sugar beets

Since the discovery of the phenomenon of haploidy, biotechnology has become an integral part in the successful creation of new varieties and hybrids of various plant species. In particular, these technologies are actively used in agriculture, which is concerned with increasing the volume and improving the quality of products. The integration of haploid production techniques together with other available biotechnological tools such as marker selection (MAS), induced mutagenesis and genetic engineering technologies can significantly accelerate crop breeding. This article shows the main stages in the development of biotechnology since 1921. Now they are successfully used to create doubled haploids to accelerate the selection process of various plants and, in particular, sugar beet, which is the most important sugar crop in regions with a temperate climate. There are several methods for obtaining forms with a single set of chromosomes. For sugar beets, the use of gynogenesis turned out to be expedient, since in this case the other methods turned out to be ineffective in the mass production of haploids. The article considers the stages of obtaining the H and DH lines of Beta vulgaris L., as well as the main stages of biotechnological production of homozygous breeding material of this culture. These stages include selecting parental forms – donor explants, sterilizing buds and introducing non-pollinated ovules in vitro, obtaining haploids, doubling their chromosome set, creating doubled haploids, determining ploidy at different stages, relocating the obtained plants to greenhouses and growing stecklings. A number of advantages that the technology of creating doubled haploids in vitro has in comparison with traditional methods of selection are described. It has been shown that the use of these approaches is relevant when obtaining new highly productive hybrids and varieties of agricultural plants; however, the methods for the production of homozygous forms in sugar beet still require additional research aimed at increasing the efficiency and reproducibility of each stage of the process

Use of Physicochemical Method for Evaluation of Mucilage Producing Ability of the Linum Usitatissimum L. Seeds

Abstract In the modern medicine of many European countries flax is used as a medicament with a wide range of use. Wholesome effect of flax seeds is determined by the large amount of enveloping substances. This property is connected with content of mucilage up to 10% and glycoside linamarin. Flaxseed polysaccharides also possess antiinflammatory effect. Furthermore, mucilage production can be a chemosystematic characteric of intraspecific taxons. In literature intervarietal variability data is limited. Therefore, comparative evaluation of mucilage producing ability of flax seeds with different morphotypes is of interest. The research of micromorphological characteristics of seed coat and mucilage production dynamics was carried out and it was established that mucilage-producing cells are localized predominantly in the external layer of seed coat. It was established that Bahmalskiy, Nebesnyj, Kustanayskiy yantar varieties possess the highest level of mucilage production. Morphotype and varietal specificity of mucilage production are determined, consequently it can be used as a marker feature of L. usitatissimum new forms. The proposed technique is based on the determination of seed physicochemical characteristics and can be used for express analysis of the vegetal samples and their differentiation by the directions of use: as a fatty oil or mucilage-containing raw material. Keywords: Linum usitatissimum L. varieties, seeds, mucilage production, hydration dynamics, physicochemical method

Comparative Analysis of Clinical, Hormonal and Morphological Studies in Patients with Neuroendocrine ACTH-Producing Tumours

This paper highlights the problem of neuroendocrine tumours (NETs) with clinical symptoms of hypercorticism caused by hypersecretion of adrenocorticotropic hormone (ACTH) by tumour cells. In most cases (85%), the tumours were localized in the pituitary gland (Cushing's disease); 15% of the patients had an extrapituitary tumour that manifest as an ectopic ACTH secretion (EAS). Comparative analysis of clinical, hormonal, histological, and immunohistochemical characteristics of pituitary and extrapituitary ACTH-secreting NET was performed. It included 46 patients with CD and 38 ones exhibiting ectopic ACTH secretion (EAS). Results of the study suggest differences between CD and EAS in terms of the severity of clinical manifestations and duration of the disease. Hormonal studies showed that EAS unlike CD was associated with high plasma ACTH and cortisol levels, late-evening salivary cortisol and daily urinary free cortisol, the absence of a 60% or greater reduction of cortisol in the HDDST test, and the presence of a low (less than 2) ACTH gradient in response to desmopressin administration with catheterization of cavernous sinuses. The study of morphofunctional characteristics of the removed NET demonstrated the ability of both pituitary and extrapituitary NETs to express ACTH as well as GH, PRL, LH, and FSH. The angiogenic markers (CD31 and VEGF) were detected with equal frequency regardless of the NET localization. The histological structure of all corticotropinomas suggested their benign origin, but extrapituitary NETs were represented by different morphological types with varying malignancy, invasiveness, and metastatic properties. A higher cell proliferation potential (Ki-67) was documented for NET in patients presenting with an ectopic ACTH secretion compared to those having corticotropinomas

Nephelometric Method for Determination of Growth Parameters of Chlorella Culture

Nephelometric method for the determination of growth parameters of chlorella culture using a photoelectric colorimeter was described. Use of photoelectric colorimeter for cell counting in suspension requires periodic calibration of meter readings using chlorella standard culture (with a certain cell concentration). Chlorella vulgaris IPPAS С-66, IPPAS С-111 and IPPAS С-2019 strains served as object of research. Density of initial in vitro suspensions (after inoculation) was 0.9 mln cells/ml. Cultivation was carried out during 12 days on a Hoagland medium with a pH of 7 (temperature of 35∘C, illumination of 10 klx). Sample selection for analysis and measurement was carried out daily, three times per day. Based on the obtained data, readings of photoelectric colorimeter KFK-3.01 were calibrated via direct count of chlorella cells quantity in Goryaev’s chamber. Use of calibration curve made it possible to reduce significantly time and error in determination of cell number in suspension cultures. The proposed technique allows counting chlorella cells in the growth and development dynamics with sufficient accuracy, high sensitivity, reproducibility and speed. It can be used for comparative determination of the growth parameters of strains in vitro, standardization of suspension cultures, semi-quantitative determination of chlorella biomass in order to predict the yield of desired product

MENOPAUSE AND SLEEP DISTURBANCES

The article contains the review of literature data of domestic and foreign researchers concerning the issues of climacteric period, in women