蛍光プローブ法によるベシクルと界面活性剤との相互作用

取得学位：博士(工学)，学位授与番号：博甲第883号，学位授与年月日：平成19年3月22

Unique incorporation behavior of amino acid-type surfactant into phospholipid vesicle membrane

金沢大学工学部The incorporation behavior of some anionic surfactants, including amino acid-type surfactants, on phospholipid vesicles was investigated. This was done by measuring the release of a vesicle-entrapped fluorescence probe and the scattered light intensities of vesicle particles in the surfactant solution as a function of surfactant concentration and time. Sodium dodecyl sulfate, sodium dodecanesulfonate, sodium dodecanoyl sarcosinate, and sodium dodecanoyl glutamate were employed in this study. All surfactants ruptured the phospholipid vesicle at around each critical micelle concentration by mixed micelle formation with phospholipid. While leakage of the fluorescence probe took place at a very low concentration in the sulfate- or sulfonate-type surfactant systems, it occurred at the concentration just below the CMC in the amino acid-type surfactant systems. Kinetic analysis of the release of the probe from the vesicles showed that the former surfactants adsorbed independently and homogeneously onto the phospholipid vesicles, while the latter surfactants were cooperatively incorporated

The escape of ionizing photons from supernova-dominated primordial galaxies

In order to assess the contribution of Lyman break galaxies (LBGs) and Lyman alpha emitters (LAEs) at redshifts 3<z<7 to the ionization of intergalactic medium (IGM), we investigate the escape fractions of ionizing photons from supernova-dominated primordial galaxies by solving the three-dimensional radiative transfer. The model galaxy is employed from an ultra-high-resolution chemodynamic simulation of a primordial galaxy by Mori & Umemura (2006),which well reproduces the observed properties of LAEs and LBGs. The total mass of model galaxy is 10^{11}M_sun. We solve not only photo-ionization but also collisional ionization by shocks. In addition, according to the chemical enrichment, we incorporate the effect of dust extinction, taking the size distributions of dust into account. As a result, we find that dust extinction reduces the escape fractions by a factor 1.5-8.5 in the LAE phase and by a factor 2.5-11 in the LBG phase, while the collisional ionization by shocks increases the escape fractions by a factor ~ 2. The resultant escape fractions are 0.07-0.47 in the LAE phase and 0.06-0.17 in the LBG phase. These results are well concordant with the recent estimations derived from the flux ratio at 1500 angstrom to 900 angstrom of LAEs and LBGs. Combining the resultant escape fractions with the luminosity functions of LAEs and LBGs, we find that high-z LAEs and LBGs can ionize the IGM at z=3-5. However, ionizing radiation from LAEs as well as LBGs falls short to ionize the IGM at z>6. That implies that additional ionization sources may required at z>6.Comment: 8 pages, 4 figures, accepted for publication in MNRA

Oscillon in Einstein-scalar system with double well potential and its properties.

The dynamical evolution of self-interacting scalar field has many nontrivial behaviors, which tell us many lessons in a nonlinear dynamics. On Minkowski spacetime, the scalar field with double well potential has localized, non-singular, time-dependent, long-lived solutions, which are called oscillons. The lifetime of the oscillon depends on the initial conditions. Furthermore, when the initial parameter is fine-tuned, oscillons can be infinitely, and type I critical behavior is observed. Here, we investigate the Einstein-scalar system with double well potential. We show that oscillons exist in this system, and discuss the behavior when the initial parameter is fine-tuned. Our results suggests that a new type of critical behavior appears in this theory

Physicochemical Characterization of the Catalytic Unit of Hammerhead Ribozyme and Its Relationship with the Catalytic Activity

The catalytic mechanism of hammerhead ribozymes (HHRzs) attracted great attention in relation to the chemical origin of life. However, the basicity (pKa) of the catalytic sites of HHRzs has not been studied so far. As a result, the investigation of the currently assumed mechanism from an experimentally derived pKa value has been impossible. In HHRzs, there exists a highly functionalized structural unit (A9-G10.1 site) with a catalytic residue (G12) for the nucleophile activation and metal ion-binding residue (G10.1). As inferred from this fact, there might be a possibility that HHRzs may utilize specific functions of the A9-G10.1 motif for the catalytic reaction. Therefore, here we studied the basicity of G12/G10.1-corresponding residues using RNA duplexes including the A9-G10.1 motif without other conserved residues of HHRzs. From the pH-titration experiments with NMR spectra, we have obtained the intrinsic basicity of the G12/G10.1-corresponding residues in the motif, with pKa > 11.5 (N1 of G12) and pKa 4.5 (N7 of G10.1) for the first time. Based on the derived irregular basicity, their correlation with a catalytic activity and a metal affinity were investigated. In total, the derived thermodynamic properties are an intrinsic nature of the exclusive catalytic unit of HHRzs, which will be an outstanding pivot point for the mechanistic analyses

Physicochemical Characterization of the Catalytic Unit of Hammerhead Ribozyme and Its Relationship with the Catalytic Activity

The catalytic mechanism of hammerhead ribozymes (HHRzs) attracted great attention in relation to the chemical origin of life. However, the basicity (pKa) of the catalytic sites of HHRzs has not been studied so far. As a result, the investigation of the currently assumed mechanism from an experimentally derived pKa value has been impossible. In HHRzs, there exists a highly functionalized structural unit (A9-G10.1 site) with a catalytic residue (G12) for the nucleophile activation and metal ion-binding residue (G10.1). As inferred from this fact, there might be a possibility that HHRzs may utilize specific functions of the A9-G10.1 motif for the catalytic reaction. Therefore, here we studied the basicity of G12/G10.1-corresponding residues using RNA duplexes including the A9-G10.1 motif without other conserved residues of HHRzs. From the pH-titration experiments with NMR spectra, we have obtained the intrinsic basicity of the G12/G10.1-corresponding residues in the motif, with pKa &gt; 11.5 (N1 of G12) and pKa 4.5 (N7 of G10.1) for the first time. Based on the derived irregular basicity, their correlation with a catalytic activity and a metal affinity were investigated. In total, the derived thermodynamic properties are an intrinsic nature of the exclusive catalytic unit of HHRzs, which will be an outstanding pivot point for the mechanistic analyses