Conserved transcriptional regulation of a cone phototransduction gene in vertebrates

AbstractcGMP-phosphodiesterase (PDE) is a key component in visual phototransduction. Rod and cone photoreceptors each produce their unique cGMP-PDE subunits. The α′ catalytic subunits are believed to be cone-specific. In this study, we report that transfection of the −132 to +139 sequence in the upstream region of the human α′-PDE gene fused to luciferase cDNA gives the highest level of reporter gene transcription in cultured retinoblastoma Y79 cells. Transgenic Xenopus laevis carrying this sequence fused to green fluorescent protein (GFP) expressed GFP in cones, suggesting a conserved regulatory mechanism for α′-PDE transcription in both human and frog

Quick-setting concrete and a method for making quick-setting concrete

This invention relates to a method for producing concrete, and more specifically, this invention relates to a method for producing quick-setting concrete while simultaneously minimizing the release of carbon dioxide to the atmosphere, said release of carbon dioxide inherent in cement production. A method for producing quick setting concrete comprises hydrating a concrete dry mixture with carbonate solution to create a slurry, and allowing the slurry to cure. The invention also provides for a quick setting concrete having a predetermined proportion of CaCO{sub 3} of between 5 and 23 weight percent of the entire concrete mixture, and whereby the concrete has a compression strength of approximately 4,000 pounds per square inch (psi) within 24 hours after pouring

Approach to the semiconductor cavity QED in high-Q regimes with q-deformed boson

The high density Frenkel exciton which interacts with a single mode microcavity field is dealed with in the framework of the q-deformed boson. It is shown that the q-defomation of bosonic commutation relations is satisfied naturally by the exciton operators when the low density limit is deviated. An analytical expression of the physical spectrum for the exciton is given by using of the dressed states of the cavity field and the exciton. We also give the numerical study and compare the theoretical results with the experimental resultsComment: 6 pages, 2 figure

Track A Basic Science

Peer Reviewedhttps://deepblue.lib.umich.edu/bitstream/2027.42/138319/1/jia218438.pd

Discovery of widespread transcription initiation at microsatellites predictable by sequence-based deep neural network

Using the Cap Analysis of Gene Expression (CAGE) technology, the FANTOM5 consortium provided one of the most comprehensive maps of transcription start sites (TSSs) in several species. Strikingly, ~72% of them could not be assigned to a specific gene and initiate at unconventional regions, outside promoters or enhancers. Here, we probe these unassigned TSSs and show that, in all species studied, a significant fraction of CAGE peaks initiate at microsatellites, also called short tandem repeats (STRs). To confirm this transcription, we develop Cap Trap RNA-seq, a technology which combines cap trapping and long read MinION sequencing. We train sequence-based deep learning models able to predict CAGE signal at STRs with high accuracy. These models unveil the importance of STR surrounding sequences not only to distinguish STR classes, but also to predict the level of transcription initiation. Importantly, genetic variants linked to human diseases are preferentially found at STRs with high transcription initiation level, supporting the biological and clinical relevance of transcription initiation at STRs. Together, our results extend the repertoire of non-coding transcription associated with DNA tandem repeats and complexify STR polymorphism