Estimating remineralized phosphate and its remineralization rate in the northern East China Sea during Summer 1997 : a snapshot study before Three-Gorges Dam construction

© The Author(s), 2016. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in Terrestrial, Atmospheric and Oceanic Sciences 27 (2016): 955-963, doi:10.3319/TAO.2016.01.24.01(Oc).The northern East China Sea (a.k.a., “The South Sea”) is a dynamic zone that exerts a variety of effects on the marine ecosystem due to Three-Gorges Dam construction. As the northern East China Sea region is vulnerable to climate forcing and anthropogenic impacts, it is important to investigate how the remineralization rate in the northern East China Sea has changed in response to such external forcing. We used an historical hydrographic dataset from August 1997 to obtain a baseline for future comparison. We estimate the amount of remineralized phosphate by decomposing the physical mixing and biogeochemical process effect using water column measurements (temperature, salinity, and phosphate). The estimated remineralized phosphate column inventory ranged from 0.8 to 42.4 mmol P m-2 (mean value of 15.2 ± 12.0 mmol P m-2). Our results suggest that the Tsushima Warm Current was a strong contributor to primary production during the summer of 1997 in the study area. The estimated summer (June - August) remineralization rate in the region before Three-Gorges Dam construction was 18 ± 14 mmol C m-2 d-1.T. Lee was supported by 2-Year Research Grant of Pusan National University. H.-C. Kim was partly supported by KOPRI project (PG15010). I.-N. Kim was partly supported by the National Research Foundation of Korea (NRF) grant funded by the Korea government (MSIP) (No. 2015R1C1A1A01052051). K.-T. Park was partly supported by KOPRI project (PE17010). J.-H. Kim was partly supported by the program of “Management of Marine Organisms Causing Ecological Disturbance and Harmful Effects” funded by KIMST/MOF. A.M. Macdonald’s contribution was supported by NOAA grant: #NA110AR4310063 and NSF grant: #OCE-1059881

Hachimoji DNA and RNA: A genetic system with eight building blocks

Reported here are DNA and RNA-like systems built from eight (hachi-) nucleotide letters (-moji) that form four orthogonal pairs. This synthetic genetic biopolymer meets the structural requirements needed to support Darwinism, including a polyelectrolyte backbone, predictable thermodynamic stability, and stereoregular building blocks that fit a Schrödinger aperiodic crystal. Measured thermodynamic parameters predict the stability of hachimoji duplexes, allowing hachimoji DNA to double the information density of natural terran DNA. Three crystal structures show that the synthetic building blocks do not perturb the aperiodic crystal seen in the DNA double helix. Hachimoji DNA was then transcribed to give hachimoji RNA in the form of a functioning fluorescent hachimoji aptamer. These results expand the scope of molecular structures that might support life, including life throughout the cosmos

An Assessment of Nutrition Practices and Attitudes in Family Child-Care Homes: Implications for Policy Implementation

Introduction: Family child-care homes (FCCHs) provide care and nutrition for millions of US children, including 28% in Rhode Island. New proposed regulations for FCCHs in Rhode Island require competencies and knowledge in nutrition. We explored nutrition-related practices and attitudes of FCCH providers in Rhode Island and assessed whether these differed by provider ethnicity or socioeconomic status of the enrolled children. Methods: Of 536 licensed FCCHs in Rhode Island, 105 randomly selected FCCH providers completed a survey about provider nutrition attitudes and practices, demographics of providers, and characteristics of the FCCH, including participation in the federal Child and Adult Care Food Program (CACFP). No differences between CACFP and non-CACFP participants were found; responses were compared by provider ethnicity using χ2 tests and multivariate models. Results: Nearly 70% of FCCHs reported receiving nutrition training only 0 to 3 times during the past 3 years; however, more than 60% found these trainings to be very helpful. More Hispanic than non-Hispanic providers strongly agreed to sitting with children during meals, encouraging children to finish their plate, and being involved with parents on the topics of healthy eating and weight. These differences persisted in multivariate models. Discussion: Although some positive practices are in place in Rhode Island FCCHs, there is room for improvement. State licensing requirements provide a foundation for achieving better nutrition environments in FCCHs, but successful implementation is key to translating policies into real changes. FCCH providers need culturally and linguistically appropriate nutrition-related training

Generation of Organ-conditioned Media and Applications for Studying Organ-specific Influences on Breast Cancer Metastatic Behavior

Breast cancer preferentially metastasizes to the lymph node, bone, lung, brain and liver in breast cancer patients. Previous research efforts have focused on identifying factors inherent to breast cancer cells that are responsible for this observed metastatic pattern (termed organ tropism), however much less is known about factors present within specific organs that contribute to this process. This is in part because of a lack of in vitro model systems that accurately recapitulate the organ microenvironment. To address this, an ex vivo model system has been established that allows for the study of soluble factors present within different organ microenvironments. This model consists of generating conditioned media from organs (lymph node, bone, lung, and brain) isolated from normal athymic nude mice. The model system has been validated by demonstrating that different breast cancer cell lines display cell-line specific and organ-specific malignant behavior in response to organ-conditioned media that corresponds to their in vivo metastatic potential. This model system can be used to identify and evaluate specific organ-derived soluble factors that may play a role in the metastatic behavior of breast and other types of cancer cells, including influences on growth, migration, stem-like behavior, and gene expression, as well as the identification of potential new therapeutic targets for cancer. This is the first ex vivo model system that can be used to study organ-specific metastatic behavior in detail and evaluate the role of specific organ-derived soluble factors in driving the process of cancer metastasis

SLEEP, PRAY, AND STATUS: RELIGIOSITY AS A MODERATOR IN THE ASSOCIATION BETWEEN SUBJECTIVE SOCIAL STATUS AND SLEEP IN AFRICAN AMERICAN YOUNG ADULTS

Abstract 2101 SLEEP, PRAY, AND STATUS: RELIGIOSITY AS A MODERATOR IN THE ASSOCIATION BETWEEN SUBJECTIVE SOCIAL STATUS AND SLEEP IN AFRICAN AMERICAN YOUNG ADULTS Elissa Kim, HS, Elizabeth A. Brown, HS, Alison Kwan, HS, Amara Craig, HS, Nataria T. Joseph, PhD, Psychology, Pepperdine University, Malibu, CA, Laurel M. Peterson, PhD, Psychology, Bryn Mawr, Bryn Mawr, PA Background: With growing importance being placed on biopsychosocial health, researchers have raised awareness about the role that subjective social status (SSS) plays in sleep. This is particularly important when examining African Americans given that they are at higher risk for experiencing poor sleep quality. However, religiosity has shown to impart lasting health benefits and behaviors that may be protective for sleep health. For example, the theory of religious coping proposes that religious behaviors can lead to adaptive stress responses that protect biopsychosocial health from the stressors of perceived low social status. Interactions between SSS and religiosity in predicting sleep quality are understudied. Objective: We aimed to examine whether SSS is associated with sleep quality and whether religiosity measured at global and momentary levels moderates this association in African American young adults. Methods: A sample of 129 healthy African American young adults completed 2 or 4 days of hourly ecological momentary assessment (EMA) surveys and a baseline survey. SSS was measured using the MacArthur Scale of Subjective Social Status, sleep quality was measured using the Pittsburgh Sleep Quality Index (PSQI), and religious behaviors were measured using recommended items from the Fetzer Institute (2003). A subsample of participants also wore an ActiGraph watch to assess objective sleep quality. Results: Lower SSS with respect to ones’ community (but not with respect to the United States population) was associated with worse sleep quality, b = -.28, p = .032, η2 = .04. Further, there was a significant interaction between SSS (with respect to the US population) and religiosity, p = .049, η2 = .06. Specifically, among those exhibiting higher SSS with respect to the US population, religious behaviors were protective, i.e., associated with better sleep quality. Results regarding momentary level religious behaviors and objective sleep will be presented. Conclusions: Results extend previous literature and suggest refinements to the theory of religious coping given that religiosity was shown to have a stronger positive effect on sleep quality among African Americans who reported having a higher perceived social status. Future research should continue to explore these factors at the momentary level to illuminate mechanisms by which these interactions unfold. LINK TO ABSTRACT: https://apps.psychosomatic.org/abstracts/previewAbstract.cfm?absid=82986&print=true&hideprint=true[10/3/2

Physical Activity Levels among Preschool-Aged Children in Family Child Care Homes: A Comparison between Hispanic and Non-Hispanic Children Using Accelerometry

Obesity prevalence among Hispanic children is twice that of non-Hispanic white children; Hispanic children may also engage in less physical activity (PA) compared to non-Hispanic white children. A large number of U.S. preschool-aged children are cared for in Family Child Care Homes (FCCH), yet few studies have examined PA levels and ethnicity differences in PA levels among these children. We examine baseline data from a cluster-randomized trial (Healthy Start/Comienzos Sanos) to improve food and PA environments in FCCHs. Children aged 2-to-5-years (n = 342) wore triaxial accelerometers for two days in FCCHs. Variables examined include percentage of time (%) spent in sedentary, and light, moderate, and vigorous PA. The full dataset (n = 342) indicated sedentary behavior 62% ± 11% of the time and only 10% ± 5% of the time spent in moderate-to-vigorous PA. Among children in the upper-median half of wear-time (n = 176), Hispanic children had significantly greater % sedentary time vs. Non-Hispanic children (66.2% ± 8.3% vs. 62.6% ± 6.9%, p = 0.007), and lower % light PA (25.4% ± 6.3% vs. 27.7% ± 4.9%, p = 0.008) and moderate PA (5.5% ± 2.1% vs. 6.4% ± 2.2%, p = 0.018). Our results highlight that PA levels were lower among our sample compared to previous studies, and that Hispanic children were more sedentary and less active compared to non-Hispanic white children

A dual function for Pex3p in peroxisome formation and inheritance

Saccharomyces cerevisiae Pex3p has been shown to act at the ER during de novo peroxisome formation. However, its steady state is at the peroxisomal membrane, where its role is debated. Here we show that Pex3p has a dual function: one in peroxisome formation and one in peroxisome segregation. We show that the peroxisome retention factor Inp1p interacts physically with Pex3p in vitro and in vivo, and split-GFP analysis shows that the site of interaction is the peroxisomal membrane. Furthermore, we have generated PEX3 alleles that support peroxisome formation but fail to support recruitment of Inp1p to peroxisomes, and as a consequence are affected in peroxisome segregation. We conclude that Pex3p functions as an anchor for Inp1p at the peroxisomal membrane, and that this function is independent of its role at the ER in peroxisome biogenesis

Tissue Tropism in Host Transcriptional Response to Members of the Bovine Respiratory Disease Complex.

Bovine respiratory disease (BRD) is the most common infectious disease of beef and dairy cattle and is characterized by a complex infectious etiology that includes a variety of viral and bacterial pathogens. We examined the global changes in mRNA abundance in healthy lung and lung lesions and in the lymphoid tissues bronchial lymph node, retropharyngeal lymph node, nasopharyngeal lymph node and pharyngeal tonsil collected at the peak of clinical disease from beef cattle experimentally challenged with either bovine respiratory syncytial virus, infectious bovine rhinotracheitis, bovine viral diarrhea virus, Mannheimia haemolytica or Mycoplasma bovis. We identified signatures of tissue-specific transcriptional responses indicative of tropism in the coordination of host's immune tissue responses to infection by viral or bacterial infections. Furthermore, our study shows that this tissue tropism in host transcriptional response to BRD pathogens results in the activation of different networks of response genes. The differential crosstalk among genes expressed in lymphoid tissues was predicted to be orchestrated by specific immune genes that act as 'key players' within expression networks. The results of this study serve as a basis for the development of innovative therapeutic strategies and for the selection of cattle with enhanced resistance to BRD

Streptavidin-Binding Peptide (SBP)-tagged SMC2 allows single-step affinity fluorescence, blotting or purification of the condensin complex

<p>Abstract</p> <p>Background</p> <p>Cell biologists face the need to rapidly analyse their proteins of interest in order to gain insight into their function. Often protein purification, cellular localisation and Western blot analyses can be multi-step processes, where protein is lost, activity is destroyed or effective antibodies have not yet been generated.</p> <p>Aim</p> <p>To develop a method that simplifies the critical protein analytical steps of the laboratory researcher, leading to easy, efficient and rapid protein purification, cellular localisation and quantification.</p> <p>Results</p> <p>We have tagged the SMC2 subunit of the condensin complex with the Streptavidin-Binding Peptide (SBP), optimising and demonstrating the efficacious use of this tag for performing these protein analytical steps. Based on silver staining, and Western analysis, SBP delivered an outstanding specificity and purity of the condensin complex. We also developed a rapid and highly specific procedure to localise SBP-tagged proteins in cells in a single step procedure thus bypassing the need for using antibodies. Furthermore we have shown that the SBP tag can be used for isolating tagged proteins from chemically cross-linked cell populations for capturing DNA-protein interactions.</p> <p>Conclusions</p> <p>The small 38-amino acid synthetic SBP offers the potential to successfully perform all four critical analytical procedures as a single step and should have a general utility for the study of many proteins and protein complexes.</p