Azole fungicide sensitivity and molecular mechanisms of reduced sensitivity in Irish Pyrenopeziza brassicae populations

© 2022 The Authors. Pest Management Science published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry. This is an open access article under the terms of the Creative Commons Attribution-Non Commercial-No Derivatives License. https://creativecommons.org/licenses/by-nc-nd/4.0/BACKGROUND: Light leaf spot, caused by Pyrenopeziza brassicae, is amongst the most damaging diseases of winter oilseed rape(Brassica napus), and currently the sterol 14⊍-demethylase (CYP51) inhibitors (azoles) represent the main class of fungicides used to control light leaf spot development. However, a shift in sensitivity to azole fungicides in P. brassicae populations has been observed in different European countries, including Ireland. RESULTS: To assess the sensitivity status of Irish P. brassicae populations to azole fungicides, three collections of P. brassicae from 2018–2020 were tested in vitro against tebuconazole and prothioconazole-desthio, and the PbCYP51 gene targeted by this class of fungicides was genotyped in different isolates. A change in sensitivity to azole fungicides was observed and differences in sensitivity to tebuconazole between Irish populations were present. There were two substitutions within PbCYP51 (G460Sand S508T) and inserts of different sizes in its promoter region. The presence of the G460S/S508T double mutant was reported for the first time, and the diversity in insert size was greater than previously known. Compared to wild type isolates, those carrying G460S or S508T were less sensitive to both fungicides and, where inserts were also identified, they further reduced sensitivity to azole fungicides. CONCLUSIONS: The results of this study suggest that azole fungicides are still very effective in controlling light leaf spot in Ireland. However, using azole fungicides in mixtures of fungicides with different modes of action is recommendedPeer reviewe

Investigation of molecular mechanisms associated with fungicide sensitivity in Irish Pyrenopeziza brassicae populations

© 2020 The Author(s). This an open access work distributed under the terms of the Creative Commons Attribution Licence (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.Light leaf spot is amongst the most damaging diseases of oilseed rape and a significant threat to Irish crops. Unfortunately, the epidemiology of Pyrenopeziza brassicae, the agent causing this disease, remains poorly understood under Irish growing conditions and fungicides are relied upon to provide control. To investigate if the cropping strategies currently used are the best for the control of disease, we screened three populations of P. brassicae isolates from different regions of Ireland for alterations in the genes targeted by azole and Methyl benzamidazole carbamate fungicides. As molecular mechanisms associated with a decrease in fungicide sensitivity were observed in the populations, the results will be correlated with data from fungicide sensitivity tests using these classes of fungicides.Non peer reviewe

Identifying the drivers and constraints to adoption of IPM amongst arable farmers in the UK and Ireland

BACKGROUND: Arable crops in temperate climatic regions such as the UK and Ireland are subject to multitude of pests (weeds, diseases and vertebrate/invertebrate pests) that can negatively impact productivity if not properly managed. Integrated pest management (IPM) is widely promoted as a sustainable approach to pest management, yet there are few recent studies assessing adoption levels and factors influencing this in arable cropping systems in the UK and Ireland. This study used an extensive farmer survey to address both these issues. RESULTS: Adoption levels of various IPM practices varied across the sample depending on a range of factors relating to both farm and farmer characteristics. Positive relationships were observed between IPM adoption and farmed area, and familiarity with IPM. Choice of pest control information sources was also found to be influential on farmer familiarity with IPM, with those who were proactive in seeking information from impartial sources being more engaged and reporting higher levels of adoption. CONCLUSION: Policies which encourage farmers to greater levels of engagement with their pest management issues and more proactive information seeking, such as through advisory professionals, more experienced peers through crop walks, open days and discussion groups should be strongly encouraged

Identifying the drivers and constraints to adoption of IPM amongst arable farmers in UK and Ireland.

Publication history: Accepted - 2 May 2021; Published online - 15 May 2021.BACKGROUND Arable crops in temperate climatic regions such as the UK and Ireland are subject to a multitude of pests (weeds, diseases and vertebrate/invertebrate pests) that can negatively impact productivity if not properly managed. Integrated pest management (IPM) is widely promoted as a sustainable approach to pest management, yet there are few recent studies assessing adoption levels and factors influencing this in arable cropping systems in the UK and Ireland. This study used an extensive farmer survey to address both these issues. RESULTS Adoption levels of various IPM practices varied across the sample depending on a range of factors relating to both farm and farmer characteristics. Positive relationships were observed between IPM adoption and farmed area, and familiarity with IPM. Choice of pest control information sources was also found to be influential on farmer familiarity with IPM, with those who were proactive in seeking information from impartial sources being more engaged and reporting higher levels of adoption. CONCLUSION Policies that encourage farmers to greater levels of engagement with their pest management issues and more proactive information seeking, such as through advisory professionals, more experienced peers through crop walks, open days and discussion groups should be strongly encouraged.We acknowledge the financial and logistical support of the: Scottish Government Strategic Research Programme; Rural Business Research (England); Department of Agriculture, Food and the Marine (Ireland) (RSF 14/S/879); and the Department of Agriculture, Environment and Rural Affairs (Northern Ireland) in enabling the study reported here

The structure of legal services markets in Scotland

In a recent Green Paper on restrictive trade practices policy [HMSO, 1988] the government suggested that those who currently occupied a privileged position in terms of restrictive trade practices would be required to justify the continuation of these privileges. The professions were identified as being in such a privileged position. Attention was particularly focused on the legal profession. This paper discusses that