Developing proteomics approaches for identifying new, redox-regulated proteins

Abstract only availableFor many years it was thought that hydrogen peroxide was only a toxic substance to cells. However, recent work has revealed that hydrogen peroxide can be utilized by organisms as a cellular signaling molecule. Hydrogen peroxide has the ability to react with proteins involved in signal transduction causing the activity of these proteins to be turned on or off. One such example is protein tyrosine phosphatase 1B (PTP1B) whose enzymatic activity is turned off by reacting with hydrogen peroxide. A cysteine residue in the active site of PTP1B is oxidized by hydrogen peroxide to form a sulfenic acid which then reacts with a neighboring amide nitrogen in the protein backbone to form a cyclic sulfenamide. The formation of this heterocycle causes PTP1B to lose its activity. In order to discover new proteins that are oxidatively regulated by hydrogen peroxide, chemical tools for selective detection of cyclic sulfenamide residues in cellular probing need to be developed. We describe results obtained using simple chemical models to identify reagents that have the ability to selectively tag protein derived sulfenamide residues.Life Sciences Undergraduate Research Opportunity Progra

670nm Photobiomodulation as a Novel Protection against Retinopathy of Prematurity:Evidence from Oxygen Induced Retinopathy Models

Introduction:To investigate the validity of using 670nm red light as a preventative treatment for Retinopathy of Prematurity in two animal models of oxygen-induced retinopathy (OIR).Materials and Methods:During and post exposure to hyperoxia, C57BL/6J mice or Sprague-Dawley rats were exposed to 670nm light for 3 minutes a day (9J/cm2). Whole mounted retinas were investigated for evidence of vascular abnormalities, while sections of neural retina were used to quantify levels of cell death using the TUNEL technique. Organs were removed, weighed and independent histopathology examination performed.Results:670nm light reduced neovascularisation, vaso-obliteration and abnormal peripheral branching patterns of retinal vessels in OIR. The neural retina was also protected against OIR by 670nm light exposure. OIR-exposed animals had severe lung pathology, including haemorrhage and oedema, that was significantly reduced in 670nm+OIR light-exposed animals. There were no significance differences in the organ weights of animals in the 670nm light-exposed animals, and no adverse effects of exposure to 670nm light were detected. Discussion: Low levels of exposure to 670nm light protects against OIR and lung damage associated with exposure to high levels of oxygen, and may prove to be a non-invasive and inexpensive preventative treatment for ROP and chronic lung disease associated with prematurity.</p

High-contrast imager for Complex Aperture Telescopes (HiCAT): 1. Testbed design

Searching for nearby habitable worlds with direct imaging and spectroscopy will require a telescope large enough to provide angular resolution and sensitivity to planets around a significant sample of stars. Segmented telescopes are a compelling option to obtain such large apertures. However, these telescope designs have a complex geometry (central obstruction, support structures, segmentation) that makes high-contrast imaging more challenging. We are developing a new high-contrast imaging testbed at STScI to provide an integrated solution for wavefront control and starlight suppression on complex aperture geometries. We present our approach for the testbed optical design, which defines the surface requirements for each mirror to minimize the amplitude-induced errors from the propagation of out-of-pupil surfaces. Our approach guarantees that the testbed will not be limited by these Fresnel propagation effects, but only by the aperture geometry. This approach involves iterations between classical ray-tracing optical design optimization, and end-to-end Fresnel propagation with wavefront control (e.g. Electric Field Conjugation / Stroke Minimization). The construction of the testbed is planned to start in late Fall 2013.Comment: Proc. of the SPIE 8864, 10 pages, 3 figures, Techniques and Instrumentation for Detection of Exoplanets V

A Process Model of the U.S. Federal Perspective on STEM

Although advocacy for better science, technology, engineering, and mathematics (STEM) education has a long and distinguished history in the United States, the recent emphasis has included strong rhetoric and concomitant funding. Policy makers legislate as though STEM is clearly defined. Yet, the concept remains nebulous, which limits the nation’s capacity to act in a strong and uniformed manner to address societal challenges. In this study, the authors used grounded theory methods to synthesize and interpret the federal perspective that defines STEM in the United States. The resulting theory is a model that includes five core processes: recruitment, recapture, retention, quality assurance, and quality control. These processes interact to support the system in achieving its goal of producing a qualified future workforce. Such a model has implications for advancing the overall goals of STEM as well as further research and development on the components of the model itself

Association of severity of primary open-angle glaucoma with serum vitamin D levels in patients of African descent.

PurposeTo study the relationship between primary open-angle glaucoma (POAG) in a cohort of patients of African descent (AD) and serum vitamin D levels.MethodsA subset of the AD and glaucoma evaluation study III (ADAGES III) cohort, consisting of 357 patients with a diagnosis of POAG and 178 normal controls of self-reported AD, were included in this analysis. Demographic information, family history, and blood samples were collected from all the participants. All the subjects underwent clinical evaluation, including visual field (VF) mean deviation (MD), central cornea thickness (CCT), intraocular pressure (IOP), and height and weight measurements. POAG patients were classified into early and advanced phenotypes based on the severity of their visual field damage, and they were matched for age, gender, and history of hypertension and diabetes. Serum 25-Hydroxy (25-OH) vitamin D levels were measured by enzyme-linked immunosorbent assay (ELISA). The association of serum vitamin D levels with the development and severity of POAG was tested by analysis of variance (ANOVA) and the paired t-test.ResultsThe 178 early POAG subjects had a visual field MD of better than -4.0 dB, and the 179 advanced glaucoma subjects had a visual field MD of worse than -10 dB. The mean (95% confidence interval [CI]) levels of vitamin D of the subjects in the control (8.02 ± 6.19 pg/ml) and early phenotype (7.56 ± 5.74 pg/ml) groups were significantly or marginally significantly different from the levels observed in subjects with the advanced phenotype (6.35 ± 4.76 pg/ml; p = 0.0117 and 0.0543, respectively). In contrast, the mean serum vitamin D level in controls was not significantly different from that of the subjects with the early glaucoma phenotype (p = 0.8508).ConclusionsIn this AD cohort, patients with advanced glaucoma had lower serum levels of vitamin D compared with early glaucoma and normal subjects

Tracking digital impact (TDI) tool.

The Tracking Digital Impact (TDI) tool is designed to help researchers, research groups, projects and institutions assess their current and future digital engagement strategies in an objective and informed way to support the development of new and improved strategies that more effectively enable good engagement with businesses, communities, the public, governing bodies and other researchers to facilitate better engagement and greater impact. The TDI tool was developed as part of a JISC funded project which focused on identifying, synthesising and embedding business, community and public (BCE) engagement best practices. The TDI tool examined the best practices at the dot.rural Digital Economies hub at the University of Aberdeen and translated those (accompanied by new guidance) into the TDI tool. Parts of this document were sourced from 'Brief Notes on Social Media for Research' by Jennifer Holden (University of Aberdeen, October, 2012). This document describes the TDI tool and its use

From Uranium Enrichment To Renewable Energy

The goal of this Science/Engineering visualization is to show how gigawatt quantities of renewable energy can be generated at former nuclear processing sites as they are repurposed into industrial scale electrical power generation stations. The breakthrough product of this research is the design of an integrated terrestrial solar/space energy receiving station that will produce “baseload” electricity 24 hours a day. This research focuses attention on a Cold War-era uranium enrichment facility located on 3,700 acres of land in a rural area of SE Ohio. This site is judged to be suitable for research leading to the first-ever combination ground-based and space-based solar energy production facility. Were this research to be successful in designing, constructing and testing a space solar power receiving antenna (rectenna) mated to the operational structures of a terrestrial photovoltaic farm, this facility (and others like it) could be transformed from an environmental hazard to a societal benefit. In the case of the former Portsmouth Gaseous Diffusion Plant (PORTS), it is projected that the site has the capability to produce as much renewable energy as it once consumed in the form of coal-produced electricity, when two plants were installed on the Ohio River to sustain its operation. Faculty Mentors Don Flournoy and Kyle Perkin

Improving conservation practice with principles and tools from systems thinking and evaluation

Achieving nature conservation goals require grappling with ‘wicked’ problems. These intractable problems arise from the complexity and dynamism of the social–ecological systems in which they are embedded. To enhance their ability to address these problems, conservation professionals are increasingly looking to the transdisciplines of systems thinking and evaluation, which provide philosophies, theories, methods, tools and approaches that show promise for addressing intractable problems in a variety of other sectors. These transdisciplines come together especially around praxis, i.e., the process by which a theory or idea is enacted, embodied or realized. We present a review and synthesis of the learnings about praxis that have emerged from The Silwood Group, a consortium of conservation professionals, professional evaluators, and complexity and systems thinkers. The Silwood Group believes that for conservation activities to achieve ambitious goals, we should benefit nature without compromising the well-being of people, and that framing a praxis for conservation in the context of social–ecological systems will provide the greatest potential for positive impact. The learnings are presented as four key principles of a ‘praxis for effective conservation’. The four principles are: (1) attend to the whole with humility; (2) engage constructively with the values, cultures, politics, and histories of stakeholders; (3) learn through evaluative, systemic enquiry, and (4) exercise wisdom in judgement and action. We also provide descriptions and references for tools and methods to support such praxis and discuss how the thinking and approaches used by conservation professionals can be transformed to achieve greater effectiveness

The Atacama Cosmology Telescope: Dusty Star-Forming Galaxies and Active Galactic Nuclei in the Southern Survey

We present a catalog of 191 extragalactic sources detected by the Atacama Cosmology Telescope (ACT) at 148 GHz and/or 218 GHz in the 2008 Southern survey. Flux densities span 14-1700 mJy, and we use source spectral indices derived using ACT-only data to divide our sources into two sub-populations: 167 radio galaxies powered by central active galactic nuclei (AGN), and 24 dusty star-forming galaxies (DSFGs). We cross-identify 97% of our sources (166 of the AGN and 19 of the DSFGs) with those in currently available catalogs. When combined with flux densities from the Australian Telescope 20 GHz survey and follow-up observations with the Australia Telescope Compact Array, the synchrotron-dominated population is seen to exhibit a steepening of the slope of the spectral energy distribution from 20 to 148 GHz, with the trend continuing to 218 GHz. The ACT dust-dominated source population has a median spectral index of 3.7+0.62-0.86, and includes both local galaxies and sources with redshifts as great as 5.6. Dusty sources with no counterpart in existing catalogs likely belong to a recently discovered subpopulation of DSFGs lensed by foreground galaxies or galaxy groups.Comment: 13 pages, 8 figures, 4 table