1,989 research outputs found
Estudio de una escultura con inscripción ibérica procedente del santuario del Cerro de los Santos
La línea de trabajo sobre imagen en la cultura ibérica ha aportado a la arqueología ibérica excelentes resultados, tanto desde el punto de vista de la dinamización teórica de la disciplina, como en su aplicación concreta al estudio y la interpretación de determinados programas iconográficos en sus contextos arqueológicos (OLMOS 1992, 1996; ARANEGUI 1997, entre otros). Uno de los sujetos de investigación de especial relevancia de esta línea ha sido la religiosidad y sus diversas manifestaciones. En este sentido, los exvotos ibéricos labrados en caliza y bronce (RUIZ BREMÓN 1989a; PRADOS1992), sin olvidar aquellos elaborados en terracota, representan un material de estudio privilegiado
X-Ray Microanalysis of Calcium Containing Organelles in Resin Embedded Tissue
The localization of calcium in cell organelles at the electron microscope level is often achieved through cytochemical techniques, and verified by X-ray microanalysis. Various methods have been used to cytochemically detect calcium or calcium-binding sites : calcium loading, calcium substitution by strontium, barium, or even lead, and calcium precipitation by oxalate, phosphate, fluoride, or pyroantimonate. Their results may have heuristic value, particularly in preliminary studies of poorly known cell types. A complementary and more physiological approach is offered by quantitative measurement of the total calcium content of organelles after cryofixation.
Resin embedding is less demanding than cryomicrotomy and gives better images : it can be used after cryosubstitution in the presence of oxalic acid. This technique was tested, and applied to several cell types
Recommended from our members
Nd:YAG laser welding experiments
Laser-beam/plume interaction experiments were conducted with a pulsed Nd:YAG laser. A high speed camera was used to study plume growth phenomena and to determine maximum plume velocities. Tests were done on four different metals: Aluminum 1100, Molybdenum, Nickel 200, and Stainless Steel 304. Previous laser welding experiments have indicated that the vapor plume ejected from the irradiated base material significantly attenuates the laser beam energy for Nickel 200 and Stainless Steel 304. To substantiate this observation, the plume was subjected to a cross flow of argon gas. Metallurgical studies showed a significant increase in weld penetration for all materials except for Aluminum. These experiments also indicated that the plume ejects normal to the base material. Thus, the specimen was tilted at different angles in an attempt to reduce laser beam attenuation. Results showed no significant increase in weld depth when the tilt angle was increased. Mass loss measurements were also performed and the experimental data were an order-of-magnitude less than those predicted by a numerical laser welding code
Recommended from our members
Towards a reliable laser spray powder deposition system through process characterization
A series of experiments have been performed to characterize the laser spray powder deposition tea-one (HAZ) in the process. Goal of these experiments was to minimize the heat affected base substrate while obtaining a maximum build-up rate of the deposited material. Response surface models have been developed to achieve this goal. These models indicate that laser irradiance and component travel speed are both important factors to be considered in optimization of this process. These models suggest that a minimum HAZ can be obtained with a maximum material build-up height by maintaining with a slow travel speed. Although these models are useful in identifying significant factor and process trends, further refinement is required for practical use in industrial applications. Weighting of the response variables used in generating the models is being considered to improve the model robustness. High speed imaging of the deposition process suggests that the powder particle size and/or size distribution affects the stability of this process
Nuclear localization and cytosolic overexpression of LASP-1 correlates with tumor size and nodal-positivity of human breast carcinoma
<p>Abstract</p> <p>Background</p> <p>LIM and SH3 protein 1 (LASP-1), initially identified from human breast cancer, is a specific focal adhesion protein involved in cell proliferation and migration, which was reported to be overexpressed in 8–12 % of human breast cancers and thought to be exclusively located in cytoplasm.</p> <p>Methods</p> <p>In the present work we analyzed the cellular and histological expression pattern of LASP-1 and its involvement in biological behavior of human breast cancer through correlation with standard clinicopathological parameters and expression of c-erbB2 (HER-2/neu), estrogen- (ER) and progesterone-receptors (PR). For this purpose immunohistochemical staining intensity and percentage of stained cells were semi-quantitatively rated to define a LASP-1 immunoreactive score (LASP-1-IRS). LASP-1-IRS was determined in 83 cases of invasive ductal breast carcinomas, 25 ductal carcinomas in situ (DCIS) and 18 fibroadenomas. Cellular LASP-1 distribution and expression pattern was visualized by immunofluorescence and confocal microscopy and assessed through separate Western blots of nuclear and cytosol preparations of BT-20, MCF-7, MDA-MB231, and ZR-75/1 breast cancer cells.</p> <p>Results</p> <p>Statistical analysis revealed that the resulting LASP-1-IRS was significantly higher in invasive carcinomas compared to fibroadenomas (p = 0.0176). Strong cytoplasmatic expression of LASP-1 was detected in 55.4 % of the invasive carcinomas, which correlated significantly with nuclear LASP-1-positivity (p = 0.0014), increased tumor size (p = 0.0159) and rate of nodal-positivity (p = 0.0066). However, levels of LASP-1 expression did not correlate with average age at time point of diagnosis, histological tumor grading, c-erbB2-, ER- or PR-expression.</p> <p>Increased nuclear localization and cytosolic expression of LASP-1 was found in breast cancer with higher tumor stage as well as in rapidly proliferating epidermal basal cells. Confocal microscopy and separate Western blots of cytosolic and nuclear preparations confirmed nuclear localization of LASP-1.</p> <p>Conclusion</p> <p>The current data provide evidence that LASP-1 is not exclusively a cytosolic protein, but is also detectable within the nucleus. Increased expression of LASP-1 in vivo is present in breast carcinomas with higher tumor stage and therefore may be related with worse prognosis concerning patients' overall survival.</p
Lasp-1 Regulates Podosome Function
Eukaryotic cells form a variety of adhesive structures to connect with their environment and to regulate cell motility. In contrast to classical focal adhesions, podosomes, highly dynamic structures of different cell types, are actively engaged in matrix remodelling and degradation. Podosomes are composed of an actin-rich core region surrounded by a ring-like structure containing signalling molecules, motor proteins as well as cytoskeleton-associated proteins
Strategies and performance of the CMS silicon tracker alignment during LHC Run 2
The strategies for and the performance of the CMS silicon tracking system alignment during the 2015–2018 data-taking period of the LHC are described. The alignment procedures during and after data taking are explained. Alignment scenarios are also derived for use in the simulation of the detector response. Systematic effects, related to intrinsic symmetries of the alignment task or to external constraints, are discussed and illustrated for different scenarios
Overexpression of LASP-1 mediates migration and proliferation of human ovarian cancer cells and influences zyxin localisation
LIM and SH3 protein 1 (LASP-1), initially identified from human breast cancer, is a specific focal adhesion protein involved in cell proliferation and migration. In the present work, we analysed the effect of LASP-1 on biology and function of human ovarian cancer cell line SKOV-3 using small interfering RNA technique (siRNA).Transfection with LASP-1-specific siRNA resulted in a reduced protein level of LASP-1 in SKOV-3 cells. The siRNA-treated cells were arrested in G2/M phase of the cell cycle and proliferation of the tumour cells was suppressed by 60–90% corresponding to around 70% of the cells being transfected successfully as seen by immunofluorescence. Moreover, transfected tumour cells showed a 40% reduced migration. LASP-1 silencing is accompanied by a reduced binding of the LASP-1-binding partner zyxin to focal contacts without changes in actin stress fibre and microtubule organisation or focal adhesion morphology as observed by immunofluorescence. In contrast, silencing of zyxin is not influencing cell migration and had neither influence on LASP-1 expression nor actin cytoskeleton and focal contact morphology suggesting that LASP-1 is necessary and sufficient for recruiting zyxin to focal contacts.The data provide evidence for an essential role of LASP-1 in tumour cell growth and migration, possibly through influencing zyxin localization
- …