124 research outputs found
WASH and biosecurity interventions for reducing burdens of infection, antibiotic use and antimicrobial resistance in animal agricultural settings: a One Health mixed methods systematic review
Background: Infection prevention and control (IPC) is recognised as essential to addressing the emergence and spread of antimicrobial resistance (AMR) in human health, food production and the environment. How best to address this through a One Health perspective remains a challenge. This systematic review addresses this gap by identifying and synthesising evidence from interventions designed to improve water, hygiene, sanitation (WASH), and biosecurity in animal agriculture and in people that live and/or work with animals. This review aimed to capture evidence of effects of all types of intervention and across different settings. Methods: We conducted a systematic search for studies that reported on WASH and biosecurity interventions with the potential to reduce the burden of infections and reliance on antibiotics in animal production for populations living with animals and/or involved in agriculture/aquaculture with a primary focus on LMICs. We searched the following databases: Web of Science, PubMed, OVID, ProQuest, Epistemonikos, Trip, AgEcon, and Cochrane Library. For articles in Spanish, we searched Scielo, BIREME, E-Revistas, Redalyc, Lilacs, AfricaPortal, IMSEAR and WPRIM. A hand search of literature was also conducted in relevant sources, and Google Scholar and Open Grey were used for grey literature. The main outcomes of interest were: (i) reduction of infections/cases, (ii) reduction of bacterial load, and (iii) reduction of antimicrobial use and AMR. We extracted data from selected studies, performed a narrative synthesis, and developed a framework. PROSPERO Registration: The protocol for the systematic review was registered at PROSPERO, registration number CRD42020162345. Findings: A total of 104 studies were included in this systematic review. The majority of studies (64/104) (61.5%) were conducted in HICs, especially in Europe and the USA. Only 13 (12.5%) studies took place in LMICs. The majority of studies (77) were animal based, followed by 12 targeting both animals and the environment, nine focused exclusively on the environment, and only one study was exclusively about humans. Most studies were conducted in poultry (36) and pigs (27), and assessed impacts on multiple types of bacteria (commonly Salmonella spp. and Campylobacter spp.). Eighty-seven (87) studies assessed impact on IPC, 3 on AMU, and 14 on AMR. The interventions were classified as follows: 57 applied biological or chemical products to eliminate pathogens; 26 modified infrastructure and apparatus; 15 were educational/behavioural and one was a structural intervention. Around 52.8% (55/104) studies included WASH interventions focused on water quality (20), water quantity (2), hygiene (30), and sanitation (3). Likewise, 47.1% (49/104) included biosecurity interventions focused on bio-management (34), bio-containment (10), and bio-exclusion (5). Positive impacts were reported for 64 (61.5%) interventions, on infection burden (54/87), antibiotic use (3/3) or AMR (7/14). The majority were non-randomised studies (55), although a quarter were randomised controlled trials (26). A total of 27 studies were classified as having low risk of bias, 21 moderate and 56 high risk of bias. Interpretation: This review identifies a number of effective interventions to reduce the burden of infections, antimicrobial resistance and antibiotic use in animal agricultural settings. Interventions which undertook bio-management and bio-containment measures appeared to have positive effects most often. These measures attempted to create and maintain a conducive environment for animal raising in terms of physical infrastructure and protocols. The few studies reporting sanitation measures - which were similar to bio-containment interventions - all reported positive effects. By contrast, efforts to impact water quantity, water quality, and hygiene had more mixed effects on the outcomes assessed. Bio-exclusion interventions had mostly negative effects. Risk of bias was high or moderate in many studies, however, and publication bias should also be considered. The paucity of studies evaluating structural interventions needs to be addressed. There are opportunities to learn from biosecurity Interventions for WASH and we propose the 'A' In WASH represents both 'Animals' and 'Air' in recognition of pathways of infection that can be addressed to also impact AMR
Role of rock fragment cover on runoff generation and sediment yield in tilled vineyards.
The soil in conventional Mediterranean vineyards is an active and non-sustainable source of sediment and water. Lack of vegetation cover, small soil organic matter content and intense ploughing result in large rates of erosion in a millennia-old tillage system. There is a need for soil conservation strategies that enable sustainability of wine and grape production; therefore, it is essential to measure the rates and to investigate the processes and factors of soil erosion. This study evaluated factors that can reduce soil losses in traditional Mediterranean vineyards. The investigation was carried out with 96 rainfall simulation experiments at the pedon scale (0.24m2) to measure soil detachment and runoff yield under low frequency-high magnitude rainfall events of 1 hour at 55mmhourâ1. On average, runoff was 40.6% of the rainfall, and the rate of soil erosion (i.e. amount of soil lost) was 71.5 g mâ2. The key factor controlling erosion was the rock fragment cover. There was a clear decrease in soil losses with increased rock fragment cover on the soil surface, but an increase in surface runoff. The results of our study showed that rock fragments at the pedon scale reduced soil erosion in Mediterranean vineyards, but when a layer of embedded rock fragments developed, large rates of runoff were triggered
An interdisciplinary framework for Islamic cognitive theories
The Islamic psychology (IP) community in Europe has recently witnessed a heated debate about the credentials required to participate in the theoretical substantiation of IP and Islamically integrated psychotherapy and counseling. This debate has provided convenient circumstances for Muslim psychologists and Islamic scholars alike to rethink their roles within the flourishing movement. Specifically, the discussions hint toward the importance of adopting a collaborative research methodology for IP, in particular for basic research. The methodology of choice will need to define the necessary qualifications and responsibilities of scholars and psychologists in a collaborative research process (personal collaboration) and evince its capability to appropriately marry knowledge and data, diverging research methods, and perspectives, concepts, and theories from Islamic studies and contemporary psychology (content-related collaboration). Here, we devise and offer a case illustration of an Islamic Psychology Basic Research Framework (coined the SALAAM Framework). This framework uses the Institute for Interdisciplinary Studies (IIS) Model of Interdisciplinary Research, developed by the IIS at the University of Amsterdam. Our first aim is to appropriate the IIS model for the IP literature by applying the model's research process phases and technique for the integration of disparate bodies of knowledgeâthat is, the identification of common groundâto methodological approaches in the contemporary IP literature. Our second aim is to exemplify the devised SALAAM Framework using the relatively unexplored area of Islamic cognitive theories (ICTs), which remain underdeveloped in contemporary psychological literature, primarily because of a lack of commensurability with the nomenclature of contemporary psychology. We thus provide a primer on the potential scope of ICTs. Toward the end of this article, we discuss the potential of the project of interdisciplinary construction of Islamic psychological theory, and the ability of the SALAAM Framework to establish a research program in IP that centers on cognition. We finally offer our reflections on the distinctiveness of Islamic psychologies in comparison to mainstream and Christian psychology.Q4WOS:0004589189000062-s2.0-8506156876
Rethinking âRational Imitationâ in 14-Month-Old Infants: A Perceptual Distraction Approach
In their widely noticed study, Gergely, Bekkering, and KirĂĄly (2002) showed that 14-month-old infants imitated an unusual action only if the model freely chose to perform this action and not if the choice of the action could be ascribed to external constraints. They attributed this kind of selective imitation to the infants' capacity of understanding the principle of rational action. In the current paper, we present evidence that a simpler approach of perceptual distraction may be more appropriate to explain their results. When we manipulated the saliency of context stimuli in the two original conditions, the results were exactly opposite to what rational imitation predicts. Based on these findings, we reject the claim that the notion of rational action plays a key role in selective imitation in 14-month-olds
Unique genome-wide transcriptome profiles of chicken macrophages exposed to Salmonella-derived endotoxin
<p>Abstract</p> <p>Background</p> <p>Macrophages play essential roles in both innate and adaptive immune responses. Bacteria require endotoxin, a complex lipopolysaccharide, for outer membrane permeability and the host interprets endotoxin as a signal to initiate an innate immune response. The focus of this study is kinetic and global transcriptional analysis of the chicken macrophage response to <it>in vitro </it>stimulation with endotoxin from <it>Salmonella </it><it>typhimurium</it>-798.</p> <p>Results</p> <p>The 38535-probeset Affymetrix GeneChip Chicken Genome array was used to profile transcriptional response to endotoxin 1, 2, 4, and 8 hours post stimulation (hps). Using a maximum FDR (False Discovery Rate) of 0.05 to declare genes as differentially expressed (DE), we found 13, 33, 1761 and 61 DE genes between endotoxin-stimulated versus non-stimulated cells at 1, 2, 4 and 8 hps, respectively. QPCR demonstrated that endotoxin exposure significantly affected the mRNA expression of <it>IL1B</it>, <it>IL6</it>, <it>IL8</it>, and <it>TLR15</it>, but not <it>IL10 </it>and <it>IFNG </it>in HD 11 cells. Ingenuity Pathway Analysis showed that 10% of the total DE genes were involved in inflammatory response. Three, 9.7, 96.8, and 11.8% of the total DE inflammatory response genes were significantly differentially expressed with endotoxin stimulation at 1, 2, 4 and 8 hps, respectively. The <it>NFKBIA, IL1B, IL8 and CCL4 </it>genes were consistently induced at all times after endotoxin treatment. <it>NLRC5 </it>(CARD domain containing, NOD-like receptor family, RCJMB04_18i2), an intracellular receptor, was induced in HD11 cells treated with endotoxin.</p> <p>Conclusions</p> <p>As above using an <it>in vitro </it>model of chicken response to endotoxin, our data revealed the kinetics of gene networks involved in host response to endotoxin and extend the known complexity of networks in chicken immune response to Gram-negative bacteria such as <it>Salmonella</it>. The induction of <it>NFKBIA, IL1B, IL8, CCL4 </it>genes is a consistent signature of host response to endotoxin over time. We make the first report of induction of a NOD-like receptor family member in response to <it>Salmonella </it>endotoxin in chicken macrophages.</p
Inhibition of NF-kB 1 (NF-kBp50) by RNA interference in chicken macrophage HD11 cell line challenged with Salmonellaenteritidis
The NF-kB pathway plays an important role in regulating the immunity response in animals. In this study, small interfering RNAs (siRNA) were used to specifically inhibit NF-kB 1 expression and to elucidate the role of NF-kB in the signal transduction pathway of the Salmonella challenge in the chicken HD11 cell line. The cells were transfected with either NF-kB 1 siRNA, glyceraldehyde 3-phosphate dehydrogenase siRNA (positive control) or the negative control siRNA for 24 h, followed by Salmonella enteritidis (SE) challenge or non-challenge for 1 h and 4 h. Eight candidate genes related to the signal pathway of SE challenge were selected to examine the effect of NF-kB 1 inhibition on their expressions by mRNA quantification. The results showed that, with a 36% inhibition of NF-kB 1 expression, gene expression of both Toll-like receptor (TLR) 4 and interleukin (IL)-6 was consistently and significantly increased at both 1 h and 4 h following SE challenge, whereas the gene expression of MyD88 and IL-1β was increased at 1 h and 4 h, respectively. These findings suggest a likely inhibitory regulation by NF-kB 1, and could lay the foundation for studying the gene network of the innate immune response of SE infection in chickens
Molecular evolution of the vertebrate TLR1 gene family - a complex history of gene duplication, gene conversion, positive selection and co-evolution
<p>Abstract</p> <p>Background</p> <p>The Toll-like receptors represent a large superfamily of type I transmembrane glycoproteins, some common to a wide range of species and others are more restricted in their distribution. Most members of the Toll-like receptor superfamily have few paralogues; the exception is the TLR1 gene family with four closely related genes in mammals TLR1, TLR2, TLR6 and TLR10, and four in birds TLR1A, TLR1B, TLR2A and TLR2B. These genes were previously thought to have arisen by a series of independent gene duplications. To understand the evolutionary pattern of the TLR1 gene family in vertebrates further, we cloned the sequences of TLR1A, TLR1B, TLR2A and TLR2B in duck and turkey, constructed phylogenetic trees, predicted codons under positive selection and identified co-evolutionary amino acid pairs within the TLR1 gene family using sequences from 4 birds, 28 mammals, an amphibian and a fish.</p> <p>Results</p> <p>This detailed phylogenetic analysis not only clarifies the gene gains and losses within the TLR1 gene family of birds and mammals, but also defines orthologues between these vertebrates. In mammals, we predict amino acid sites under positive selection in TLR1, TLR2 and TLR6 but not TLR10. We detect co-evolution between amino acid residues in TLR2 and the other members of this gene family predicted to maintain their ability to form functional heterodimers. In birds, we predict positive selection in the TLR2A and TLR2B genes at functionally significant amino acid residues. We demonstrate that the TLR1 gene family has mostly been subject to purifying selection but has also responded to directional selection at a few sites, possibly in response to pathogen challenge.</p> <p>Conclusions</p> <p>Our phylogenetic and structural analyses of the vertebrate TLR1 family have clarified their evolutionary origins and predict amino acid residues likely to be important in the host's defense against invading pathogens.</p
Human Genome-Wide RNAi Screen for Host Factors That Modulate Intracellular Salmonella Growth
Salmonella enterica is a bacterial pathogen of humans that can proliferate within epithelial cells as well as professional phagocytes of the immune system. While much has been learned about the microbial genes that influence the infectious process through decades of intensive research, relatively little is known about the host factors that affect infection. We performed a genome-wide siRNA screen to identify host genes that Salmonella enterica serovar Typhimurium (S. typhimurium) utilizes to facilitate growth within human epithelial cells. In this screen, with siRNAs targeting every predicted gene in the human genome, we identified 252 new human-host-susceptibility factors (HSFs) for S. typhimurium. We also identified 39 genes whose silencing results in increased intracellular growth of S. typhimurium. The HSFs identified are regulated most centrally by NFÎşB and associate with each other through an extremely dense network of interactions that center around a group of kinases. Most genes identified were not previously appreciated as playing roles in the intracellular lifecycle of S. enterica. Numerous HSFs identified with interesting characteristics that could play plausible roles in mediating intracellular microbial growth are discussed. Importantly, this study reveals significant overlap between the host network that supports S. typhimurium growth within human epithelial cells and the one that promotes the growth of Mycobacterium tuberculosis within human macrophages. In addition to providing much new information about the molecular mechanisms underlying S. enterica-host cell interplay, all 252 HSFs identified are candidates for new anti-microbial targets for controlling S. enterica infections, and some may provide broad-spectrum anti-microbial activity
The cytoskeleton in cell-autonomous immunity: structural determinants of host defence
Host cells use antimicrobial proteins, pathogen-restrictive compartmentalization and cell death in their defence against intracellular pathogens. Recent work has revealed that four components of the cytoskeleton â actin, microtubules, intermediate filaments and septins, which are well known for their roles in cell division, shape and movement â have important functions in innate immunity and cellular self-defence. Investigations using cellular and animal models have shown that these cytoskeletal proteins are crucial for sensing bacteria and for mobilizing effector mechanisms to eliminate them. In this Review, we highlight the emerging roles of the cytoskeleton as a structural determinant of cell-autonomous host defence
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