Neurotoxic Alkaloids: Saxitoxin and Its Analogs

Saxitoxin (STX) and its 57 analogs are a broad group of natural neurotoxic alkaloids, commonly known as the paralytic shellfish toxins (PSTs). PSTs are the causative agents of paralytic shellfish poisoning (PSP) and are mostly associated with marine dinoflagellates (eukaryotes) and freshwater cyanobacteria (prokaryotes), which form extensive blooms around the world. PST producing dinoflagellates belong to the genera Alexandrium, Gymnodinium and Pyrodinium whilst production has been identified in several cyanobacterial genera including Anabaena, Cylindrospermopsis, Aphanizomenon Planktothrix and Lyngbya. STX and its analogs can be structurally classified into several classes such as non-sulfated, mono-sulfated, di-sulfated, decarbamoylated and the recently discovered hydrophobic analogs—each with varying levels of toxicity. Biotransformation of the PSTs into other PST analogs has been identified within marine invertebrates, humans and bacteria. An improved understanding of PST transformation into less toxic analogs and degradation, both chemically or enzymatically, will be important for the development of methods for the detoxification of contaminated water supplies and of shellfish destined for consumption. Some PSTs also have demonstrated pharmaceutical potential as a long-term anesthetic in the treatment of anal fissures and for chronic tension-type headache. The recent elucidation of the saxitoxin biosynthetic gene cluster in cyanobacteria and the identification of new PST analogs will present opportunities to further explore the pharmaceutical potential of these intriguing alkaloids

Planetary Defense Ground Zero: MASCOT's View on the Rocks - an Update between First Images and Sample Return

At 01:57:20 UTC on October 3rd, 2018, after 3½ years of cruise aboard the JAXA spacecraft HAYABUSA2 and about 3 months in the vicinity of its target, the MASCOT lander was separated successfully by from an altitude of 41 m. After a free-fall of only ~5m51s MASCOT made first contact with C-type near-Earth and potentially hazardous asteroid (162173) Ryugu, by hitting a big boulder. MASCOT then bounced for ~11m3s, in the process already gathering valuable information on mechanical properties of the surface before it came to rest. It was able to perform science measurements at 3 different locations on the surface of Ryugu and took many images of its spectacular pitch-black landscape. MASCOT’s payload suite was designed to investigate the fine-scale structure, multispectral reflectance, thermal characteristics and magnetic properties of the surface. Somewhat unexpectedly, MASCOT encountered very rugged terrain littered with large surface boulders. Observing in-situ, it confirmed the absence of fine particles and dust as already implied by the remote sensing instruments aboard the HAYABUSA2 spacecraft. After some 17h of operations, MASCOT‘s mission ended with the last communication contact as it followed Ryugu’s rotation beyond the horizon as seen from HAYABUSA2. Soon after, its primary battery was depleted. We present a broad overview of the recent scientific results of the MASCOT mission from separation through descent, landing and in-situ investigations on Ryugu until the end of its operation and relate them to the needs of planetary defense interactions with asteroids. We also recall the agile, responsive and sometimes serendipitous creation of MASCOT, the two-year rush of building and delivering it to JAXA’s HAYABUSA2 spacecraft in time for launch, and the four years of in-flight operations and on-ground testing to make the most of the brief on-surface mission

Lysyl Oxidase-like-2 (LOXL2) Is a Major Isoform in Chondrocytes and Is Critically Required for Differentiation*

The lysyl oxidase family is made up of five members: lysyl oxidase (LOX) and lysyl oxidase-like 1–4 (LOXL1-LOXL4). All members share conserved C-terminal catalytic domains that provide for lysyl oxidase or lysyl oxidase-like enzyme activity; and more divergent propeptide regions. LOX family enzyme activities catalyze the final enzymatic conversion required for the formation of normal biosynthetic collagen and elastin cross-links. The importance of lysyl oxidase enzyme activity to normal bone development has long been appreciated, but regulation and roles for specific LOX isoforms in bone formation in vivo is largely unexplored. Fracture healing recapitulates aspects of endochondral bone development. The present study first investigated the expression of all LOX isoforms in fracture healing. A remarkable coincidence of LOXL2 expression with the chondrogenic phase of fracture healing was found, prompting more detailed analyses of LOXL2 expression in normal growth plates, and LOXL2 expression and function in developing ATDC5 chondrogenic cells. Data show that LOXL2 is expressed by pre-hypertrophic and hypertrophic chondrocytes in vivo, and that LOXL2 expression is regulated in vitro as a function of chondrocyte differentiation. Moreover, LOXL2 knockdown studies in vitro show that LOXL2 expression is required for ATDC5 chondrocyte cell line differentiation through regulation of SNAIL and SOX9, important transcription factors that control chondrocyte differentiation. Taken together, data provide evidence that LOXL2, like LOX, is a multifunctional protein. LOXL2 promotes chondrocyte differentiation by mechanisms that are likely to include roles as both a regulator and an effector of chondrocyte differentiation

MASCOT operations on Ryugu - focus on some specific tasks

Hayabusa2 is an asteroid sample return mission operated by the Japanese space agency, JAXA. It was launched in December 2014. In July 2018, the spacecraft has reached the mission target after a 4-year-long cruise. The objective is a C-type primordial asteroid called Ryugu, in search of organic and hydrated minerals that might give essential clues for the solar system formation. The small lander MASCOT (Mobile Asteroid surface SCOuT) carried aboard Hayabusa2 landed on the surface on the 3rd of October 2018 for reliminary in-situ investigations while the probe is aiming to study Ryugu on a global scale and to return samples to Earth. MASCOT was jointly developed by the German Aerospace Centre (DLR) and the Centre National d'Etudes Spatiales (CNES). It is equipped with a sensor suite consisting of four fully-fledged instruments. DLR was responsible for developing the MASCOT lander and ground segment, and was in charge of planning and conducting lander joint operations from MUSC. CNES supplied the hyperspectral IR spectrometer (MicrOmega, IAS Paris), antennae and power system, provided a support to operations and was in charge of the flight dynamics aspects of the mission. The 17 hours of on-asteroid operations exceeded expectations and the overall landing and operations were a huge success. Indeed, the characteristics of the Ryugu asteroid such as the shape and the gravity were known only after arrival of Hayabusa2 in July 2018 and the operating ccontext was very constrained but did not provide from fulfilling the objectives. This paper is a complement to the overall paper on MASCOT landing and first results. It will focus on several operational tasks such as communication and power subsystems assessments as well as flight dynamics computations needed in real time and for postprocessing

MASCOT - a Mobile Lander on-board Hayabusa2 Spacecraft - Operations and Status after Launch

MASCOT (‘Mobile Asteroid Surface Scout’) is a 10 kg mobile surface science package on board JAXA’s Hayabusa2 spacecraft, currently on its way to the near-Earth asteroid (162173) Ryugu. MASCOT has been developed by the German Aerospace Center (DLR) in cooperation with the Centre National d’Etudes Spatiales (CNES). The concept of MASCOT is to perform in-situ measurements on the asteroid’s surface and to support the Hayabusa2 mission in the sampling site selection. MASCOT is equipped with 4 scientific instruments, a wide angle camera, a hyperspectral IR microscope, a radiometer and a magnetometer. MASCOT is powered by a primary battery which shall enable MASCOT to investigate the asteroid surface for up to 2 asteroid days. An internal mobility mechanism shall relocate MASCOT on the asteroid surface to investigate different landing sites in detail

MASCOT - a Mobile Lander on-board Hayabusa2 Spacecraft - Status and Operational Concept for the Asteroid Ryugu

MASCOT (‘Mobile Asteroid Surface Scout’) is a 10 kg mobile surface science package on board JAXA’s Hayabusa2 sample return mission, currently on its way to the near-Earth asteroid (162173) Ryugu. The mission was launched in December 2014 from Tanegashima Space Center, Japan. The Hayabusa2 spacecraft will reach the target asteroid in summer 2018. Hayabusa2 will return its samples to Earth in December 2020. After arrival at the target asteroid ‘Ryugu’ a detailed mapping phase will be performed and the landing site of MASCOT will be selected. The deployment of MASCOT to the asteroids surface is planned for the beginning of October 2018. MASCOT has been developed by the German Aerospace Center (DLR) in cooperation with the Centre National d’Etudes Spatiales (CNES). The main objective of MASCOT is to perform in-situ investigations of the asteroid surface and to support the sampling site selection for the mother spacecraft. Mascot is equipped with four scientific instruments a wide angle camera, a hyperspectral infrared microscope, a radiometer and a magnetometer. The camera (MasCam) provides ground truth for the orbital measurements of the Hayabusa2 orbiter instruments and the in-situ MASCOT sensor suite as well as geological context of the samples. The radiometer (MARA) determines the surface brightness temperature, the thermal inertia of the surface material and the spectral slope in infrared. The radiometer field of view is correlated with the wide angle camera field of view. The magnetometer (MasMAG) observes the magnetic field profile during descent and bouncing and determines any global and local magnetization of the asteroid

MASCOT—A Mobile Lander On-board the Hayabusa2 Spacecraft—Operations on Ryugu

MASCOT (‘Mobile Asteroid Surface Scout’) is a 10 kg mobile surface science package part of JAXA’s Hayabusa2 sample return mission. The mission was launched in December 2014 from Tanegashima Space Center, Japan. The Hayabusa2 spacecraft reached the target asteroid in summer 2018. After a mapping phase of the asteroid and a landing site selection process the MASCOT lander was deployed to the surface on the 3rd of October 2018. MASCOT operated successfully for about 17 h on the surface of Ryugu. It performed three relocation manoeuvres and one “Mini-Move” and returned 128 MBytes of data. MASCOT has been developed by the German Aerospace Center (DLR) in cooperation with the Centre National d’Etudes Spatiales (CNES). The main objectives were to perform in-situ investigations of the asteroid surface and to support the sampling site selection for the mother spacecraft. These objectives could be reached successfully. On 6th December 2020 Hayabusa2 successfully returned asteroid samples to the Earth