Lonely Lockdown. Life for siblings of disabled children in the UK

In May 2020, Sibs conducted a survey of parents in order to better understand how the needs of siblings of disabled children were affected by lockdown. The first survey results made difficult reading: 75% of parents felt their sibling child’s mental health had worsened in lockdown; 50% of siblings were providing more care; a third were missing family and friends. Ten months on and in lockdown 3, we wanted to explore if and how things had changed for siblings of disabled children. Sibs Charity, in collaboration with University College London (UCL) researcher Dr Georgia Pavlopoulou launched a survey in February 2021 for 4 weeks to hear from parents about family experiences with a focus on siblings' day-to-day experiences and mental health needs. We also wanted to understand the nature of any school support, how the relationships between siblings and their brothers and sisters had been affected and what some of the greatest challenges were for children and young people. We hope the results of this survey will help to highlight the impact which lockdown has had on the majority of siblings and go on to inform a recovery response. Our findings suggest that the pandemic has had both an immediate and lasting effect on the mental health of many siblings who will need access to appropriate support from CAMHS services and schools in the months and years to come as part of a recovery plan

Analisis Nilai Tambah Bawang Merah Lokal Palu Menjadi Bawang Goreng di Kota Palu

The role of agroindustry to maintain the primary product into processing products to increase the added value is required. One of the solutions made is to increase the added value of local Palu onion become fried onions. This study aims to determine the added value of being processing the local Palu onion into fried onion which was conducted on Triple C Fried Onion Industry, Raja Bawang Fried Onion Industry and SAL-HAN Fried Onion Industry. The samples determined by intentionally (purposive), considering that these 3 industries categorized as home industry, small industry and middle industry. Respondents in this study was the leadership and employees of the company with a number of respondents as many as 11 people. The results showed that the added value obtained from fried Onion Industry at home level (Triple C fried onions Industry) of Rp.33.846.09 kg , small scale (Raja Bawang Fried Onion Industry) of Rp .39.117,40/kg and middle scale (SAL-HA Fried Onion Industry) of Rp.39.760/k

Occurrence of bovine hydatidosis and evaluation of its risk to humans in traditional communities of Southern Region of Ethiopia

Background: Cystic Echinococcosis/ Hydatid Disease, is an infection caused by the larval stage of the tapeworm Echinococcus granulosus, one of the most widespread parasitic zoonoses.Objective: To determine the occurrence, localization and fertility/sterility rates of hydatid cyst in cattle, to determine the prevalence of adult E. granulosus in dogs and asses the risk for human infection in traditional communities.Methods: Postmortem examination, hydatid cyst characterization,  questionnaire survey and dog stool sample examination were carried out.Results: Of the total 320 ruminants examined at backyard slaughtering, 151 (47.2%) were found harboring hydatid cysts. The liver and lungs were the two main infected organs, 34.4% and 52.3%, respectively. The majority of the cysts found were small, 54.3%, and medium, 37.7%. From the total number of cysts found, 70.2% were sterile, while 29.8% were fertile. A questionnaire survey revealed that local people were unaware of the life cycle of E. granulosus and the perpetuation of its life cycle by their cultural and traditional practices. Dogs have intimate contact with humansand other domestic animals, share the same house and also dogs do not have access for veterinary care. Stool samples of 62 dogs were collected and analyzed with a 30% prevalence of taenia infection confirmed to be E. granulosus.Conclusion: Because of the high prevalence of E. granulosus infection in dogs and hydatidosis in cattle as well as common practice of backyard slaughtering, the risk of human infection in traditional communities is suspected to be high and requires immediate attention to study the status of cystic ehinococcosis in the human population of the study area.[Ethiop. J. Health Dev. 2012;26(1):43-48

A comparison of the neuroprotective efficacy of newly developed oximes (K117, K127) and currently available oxime (obidoxime) in tabun-poisoned rats

The potency of newly developed bispyridinium compounds (K117, K127) to reduce tabun-induced acute neurotoxic signs and symptoms was compared with currently available oxime (obidoxime) using functional observational battery. The neuroprotective effects of atropine alone and atropine combined with one of three bispyridinium oximes (K117, K127, obidoxime) on rats poisoned with tabun at a sublethal dose (180 μg/kg i.m.; 80% of LD50 value) were studied. Tabun-induced neurotoxicity was monitored using a functional observational battery and automatic measurement of motor activity at 24 h following tabun challenge. The results indicated that all tested oximes combined with atropine enabled tabun-poisoned rats to survive 24 h following tabun challenge while one tabun-poisoned rats died within 24 h after tabun poisoning when the rats were treated with atropine alone. Newly developed oxime K127 combined with atropine was the most effective in decreasing tabun-induced neurotoxicity in the case of sublethal poisonings among all oximes tested. Nevertheless, the differences of neuroprotective efficacy between K127 and obidoxime are not sufficient to replace obidoxime by K127 for the treatment of acute tabun poisonings

A four-month gatifloxacin-containing regimen for treating tuberculosis.

BACKGROUND: Shortening the course of treatment for tuberculosis would be a major improvement for case management and disease control. This phase 3 trial assessed the efficacy and safety of a 4-month gatifloxacin-containing regimen for treating rifampin-sensitive pulmonary tuberculosis. METHODS: We conducted a noninferiority, randomized, open-label, controlled trial involving patients 18 to 65 years of age with smear-positive, rifampin-sensitive, newly diagnosed pulmonary tuberculosis in five sub-Saharan African countries. A standard 6-month regimen that included ethambutol during the 2-month intensive phase was compared with a 4-month regimen in which gatifloxacin (400 mg per day) was substituted for ethambutol during the intensive phase and was continued, along with rifampin and isoniazid, during the continuation phase. The primary efficacy end point was an unfavorable outcome (treatment failure, recurrence, or death or study dropout during treatment) measured 24 months after the end of treatment, with a noninferiority margin of 6 percentage points, adjusted for country. RESULTS: A total of 1836 patients were assigned to the 4-month regimen (experimental group) or the standard regimen (control group). Baseline characteristics were well balanced between the groups. At 24 months after the end of treatment, the adjusted difference in the risk of an unfavorable outcome (experimental group [21.0%] minus control group [17.2%]) in the modified intention-to-treat population (1356 patients) was 3.5 percentage points (95% confidence interval, -0.7 to 7.7). There was heterogeneity across countries (P=0.02 for interaction, with differences in the rate of an unfavorable outcome ranging from -5.4 percentage points in Guinea to 12.3 percentage points in Senegal) and in baseline cavitary status (P=0.04 for interaction) and body-mass index (P=0.10 for interaction). The standard regimen, as compared with the 4-month regimen, was associated with a higher dropout rate during treatment (5.0% vs. 2.7%) and more treatment failures (2.4% vs. 1.7%) but fewer recurrences (7.1% vs. 14.6%). There was no evidence of increased risks of prolongation of the QT interval or dysglycemia with the 4-month regimen. CONCLUSIONS: Noninferiority of the 4-month regimen to the standard regimen with respect to the primary efficacy end point was not shown. (Funded by the Special Program for Research and Training in Tropical Diseases and others; ClinicalTrials.gov number, NCT00216385.)

Evaluation of Two Estrus Synchronization Protocols in Dairy Cattle at North Shoa Zone Ethiopia

This study was initiated to change the hesitation of the farmer on the effectiveness of estrus synchronization under their (Ethiopian small holder) livestock management system using two synchronization protocols. Non-pregnant animals with normal reproductive tract and that fulfilled the preconditions for estrus synchronization were considered for treatment & assigned into two synchronization protocols (single PGF2α injection; and double PGF2α injection). Among 94 (27 heifer and 67 cows) synchronized cows using one and two injections of PGF2α protocols 26 heifers and 63 cows (89/94.7%) were exhibited estrus by visual observation and rectal palpation the remaining 5 (5.3%) did not illustrate heat. The overall pregnancy was 59.6 % with overall birth 94.3 %. High pregnancy was obtained in the double injection of PGF2α treatment group (63.1 %) than animals treated with one shot protocol 55.8 % there were statistically significant difference between treatments (p<0.05). Higher pregnancy was obtained from cross breed animals than local breeds. More over most of the animals come to estrus greater than 96 hrs. There was also significant difference between technicians on detecting the CL and conception. The estrus response, conception rate, pregnancy rate and calving rate was higher in both protocols so producers or farmers can use either the two protocols to achieve remarkable result but tight follow-ups and more resources are need to be exploited at farmer level

Phenotypic cha- racterization of pig genetic resources in the departments of Oueme and Plateau in Benin

L’élevage porcin est très pratiqué au Sud-Bénin et implique une diversité de races ou de populations. L’objectif de l’étude était de caractériser les différents porcs sur le plan morphométrique et phénotypique. Ainsi, les données phéno- typiques ont été collectées sur 149 porcs, dont 14 améliorés, 91 croisés et 44 locaux. Les porcs de type génétique local ont présenté des mesures morpho- logiques significativement inférieures (p < 0,05) à celles des porcs améliorés et des animaux issus des croisements entre les porcs améliorés et les porcs locaux. Les poils des porcs locaux étaient significativement plus courts (p < 0,05) que ceux des porcs améliorés et des croisés. La couleur de la robe a varié d’un type génétique à l’autre. La couleur la plus rencontrée a été le blanc uniforme, suivi du noir uniforme chez tous les types génétiques. Le profil de la tête était plus rectiligne chez les porcs locaux, et plus concave chez les porcs améliorés et chez les croisés. Les oreilles dressées étaient moins observées chez les croisés. Elles étaient orientées vers l’avant chez les porcs améliorés et chez les croisés alors qu’elles étaient dressées et orientées vers l’arrière chez les porcs locaux. La queue en tire-bouchon a été significativement (p < 0,05) plus présente chez les porcs améliorés que chez les porcs croisés, et elle a été plus présente chez ces derniers que chez les porcs locaux. La ligne dorsale droite a été davantage observée chez les porcs locaux et chez les croisés que chez les porcs amélio- rés chez lesquels la ligne était plus creuse. Les porcs croisés ont présenté une grande similarité avec les porcs améliorés

Gene expression profiles classifying clinical stages of tuberculosis and monitoring treatment responses in Ethiopian HIV-negative and HIV-positive cohorts.

BACKGROUND: Validation of previously identified candidate biomarkers and identification of additional candidate gene expression profiles to facilitate diagnosis of tuberculosis (TB) disease and monitoring treatment responses in the Ethiopian context is vital for improving TB control in the future. METHODS: Expression levels of 105 immune-related genes were determined in the blood of 80 HIV-negative study participants composed of 40 active TB cases, 20 latent TB infected individuals with positive tuberculin skin test (TST+), and 20 healthy controls with no Mycobacterium tuberculosis (Mtb) infection (TST-), using focused gene expression profiling by dual-color Reverse-Transcription Multiplex Ligation-dependent Probe Amplification assay. Gene expression levels were also measured six months after anti-TB treatment (ATT) and follow-up in 38 TB patients. RESULTS: The expression of 15 host genes in TB patients could accurately discriminate between TB cases versus both TST+ and TST- controls at baseline and thus holds promise as biomarker signature to classify active TB disease versus latent TB infection in an Ethiopian setting. Interestingly, the expression levels of most genes that markedly discriminated between TB cases versus TST+ or TST- controls did not normalize following completion of ATT therapy at 6 months (except for PTPRCv1, FCGR1A, GZMB, CASP8 and GNLY) but had only fully normalized at the 18 months follow-up time point. Of note, network analysis comparing TB-associated host genes identified in the current HIV-negative TB cohort to TB-associated genes identified in our previously published Ethiopian HIV-positive TB cohort, revealed an over-representation of pattern recognition receptors including TLR2 and TLR4 in the HIV-positive cohort which was not seen in the HIV-negative cohort. Moreover, using ROC cutoff ≥ 0.80, FCGR1A was the only marker with classifying potential between TB infection and TB disease regardless of HIV status. CONCLUSIONS: Our data indicate that complex gene expression signatures are required to measure blood transcriptomic responses during and after successful ATT to fully diagnose TB disease and characterise drug-induced relapse-free cure, combining genes which resolve completely during the 6-months treatment phase of therapy with genes that only fully return to normal levels during the post-treatment resolution phase

Dryland restoration successes in the Sahel and Greater Horn of Africa show how to increase scale and impact

Drylands occupy more than 40% of the world’s land area and are home to some two billion people. This includes a disproportionate number of the world’s poorest people, who live in degraded and severely degraded landscapes. The United Nations Convention to Combat Desertification states on its website that 12 million hectares are lost annually to desertification and drought, and that more than 1.5 billion people are directly dependent on land that is being degraded, leading to US$42 billion in lost earnings each year. In Africa, three million hectares of forest are lost annually, along with an estimated 3% of GDP, through depleted soils. The result is that two-thirds of Africa’s forests, farmlands and pastures are now degraded. This means that millions of Africans have to live with malnutrition and poverty, and in the absence of options this further forces the poor to overexploit their natural resources to survive. This in turn intensifies the effects of climate change and hinders economic development, threatening ecological functions that are vital to national economies

Analysis of host responses to Mycobacterium tuberculosis antigens in a multi-site study of subjects with different TB and HIV infection states in sub-Saharan Africa.

BACKGROUND: Tuberculosis (TB) remains a global health threat with 9 million new cases and 1.4 million deaths per year. In order to develop a protective vaccine, we need to define the antigens expressed by Mycobacterium tuberculosis (Mtb), which are relevant to protective immunity in high-endemic areas. METHODS: We analysed responses to 23 Mtb antigens in a total of 1247 subjects with different HIV and TB status across 5 geographically diverse sites in Africa (South Africa, The Gambia, Ethiopia, Malawi and Uganda). We used a 7-day whole blood assay followed by IFN-γ ELISA on the supernatants. Antigens included PPD, ESAT-6 and Ag85B (dominant antigens) together with novel resuscitation-promoting factors (rpf), reactivation proteins, latency (Mtb DosR regulon-encoded) antigens, starvation-induced antigens and secreted antigens. RESULTS: There was variation between sites in responses to the antigens, presumably due to underlying genetic and environmental differences. When results from all sites were combined, HIV- subjects with active TB showed significantly lower responses compared to both TST(-) and TST(+) contacts to latency antigens (Rv0569, Rv1733, Rv1735, Rv1737) and the rpf Rv0867; whilst responses to ESAT-6/CFP-10 fusion protein (EC), PPD, Rv2029, TB10.3, and TB10.4 were significantly higher in TST(+) contacts (LTBI) compared to TB and TST(-) contacts fewer differences were seen in subjects with HIV co-infection, with responses to the mitogen PHA significantly lower in subjects with active TB compared to those with LTBI and no difference with any antigen. CONCLUSIONS: Our multi-site study design for testing novel Mtb antigens revealed promising antigens for future vaccine development. The IFN-γ ELISA is a cheap and useful tool for screening potential antigenicity in subjects with different ethnic backgrounds and across a spectrum of TB and HIV infection states. Analysis of cytokines other than IFN-γ is currently on-going to determine correlates of protection, which may be useful for vaccine efficacy trials