Spectral analysis of molecular resonances in erbium isotopes: Are they close to semi-Poisson?

We perform a thorough analysis of the spectral statistics of experimental molecular resonances, of bosonic erbium $^{166}$Er and $^{168}$Er isotopes, produced as a function of magnetic field($B$) by Frisch et al. [Nature 507, (2014) 475], utilizing some recently derived surmises which interpolate between Poisson and GOE and without unfolding. Supplementing this with an analysis using unfolded spectrum, it is shown that the resonances are close to semi-Poisson distribution. There is an earlier claim of missing resonances by Molina et al. [Phys. Rev. E 92, (2015) 042906]. These two interpretations can be tested by more precise measurements in future experiments.Comment: 7 pages, 6 figure

Energy level statistics of interacting trapped bosons

It is an well established fact that statistical properties of energy level spectra are the most efficient tool to characterize nonintegrable quantum systems. The study of statistical properties and spectral fluctuation in the interacting many boson systems have developed a new interest in this direction. Specially we are interested in the weakly interacting trapped bosons in the context of Bose-Einstein condensation (BEC) as the energy spectrum shows a transition from the collective to single particle nature with the increase in the number of levels. However this has received less attention as it is believed that the system may exhibit Poisson like fluctuations due to the existence of external harmonic trap. Here we compute numerically the energy levels of the zero-temperature many-boson systems which are weakly interacting through the van der Waals potential and are in the 3D confined harmonic potential. We study the nearest neighbour spacing distribution and the spectral rigidity by unfolding the spectrum. It is found that increase in number of energy levels for repulsive BEC induces a transition from a Wigner like form displaying level repulsion to Poisson distribution for P(s). It does not follow the GOE prediction. For repulsive interaction, the lower levels are correlated and manifest level repulsion. For intermediate levels P (s) shows mixed statistic which clearly signifies the existence of two energy scales: external trap and interatomic interaction. Whereas for very high levels the trapping potential dominates, genarating Poisson distribution. Comparison with mean-field results for lower levels are also presented. For attractive BEC near the critical point we observe the Shrielman like peak near s=0 which signifies the presence of large number of quasi-degenerate states.Comment: 12 page

Sustainable Generation of Ni(OH)2 Nanoparticles for the Green Synthesis of 5-Substituted 1 H-Tetrazoles:A Competent Turn on Fluorescence Sensing of H2O2

A mutually correlated green protocol has been devised that originates from a sustainable production of β-Ni(OH)2 nanoparticles which is used for an efficient catalytic synthesis of versatile substituted tetrazoles, under mild reaction conditions in water via a simple, one-pot, eco-friendly method. The synthesis is followed by derivatization into a highly fluorescence active compound 9-(4-(5-(quinolin-2-yl)-1H-tetrazol-1-yl)phenyl)-9H-carbazole that can be used at tracer concentrations (0.1 μM) to detect as well as quantify hydrogen peroxide down to 2 μM concentration. The nanocatalyst was synthesized by a simple, proficient, and cost-effective methodology and characterized thoroughly by UV-vis absorption and Fourier transform infrared spectra, N2 adsorption/desorption, high resolution transmission electron microscopy, powder X-ray diffraction pattern, field emission scanning electron microscopy, and thermogravimetric analysis. Broad substrate scope, easy handling, higher efficiency, low cost, and reusability of the catalyst are some of the important features of this heterogeneous catalytic system. The strong analytical performance of the resultant derivative in low-level quantification of potentially hazardous hydrogen peroxide is the key success of the overall green synthesis procedure reported here

Spectral fluctuation and 1fα\frac{1}{f^{\alpha}} noise in the energy level statistics of interacting trapped bosons

It has been recently shown numerically that the transition from integrability to chaos in quantum systems and the corresponding spectral fluctuations are characterized by $\frac{1}{f^{\alpha}}$ noise with $1\leq\alpha\leq 2$. The system of interacting trapped bosons is inhomogeneous and a complex system. The presence of external harmonic trap makes it more interesting as in the atomic trap the bosons occupy partly degenerate single-particle states. Earlier theoretical and experimental results show that at zero temperature the low-lying levels are of collective nature and high-lying excitations are of single particle nature. We observe that for few bosons, $P(s)$ distribution shows the Shnirelman peak which exhibits a large number of quasi-degenerate states. For large number of bosons the low-lying levels are strongly affected by the interatomic interaction and the corresponding level fluctuation shows a transition to Wigner with increase in particle number. It does not follow GOE (Gaussian Orthogonal Ensemble) Random Matrix predictions. For high-lying levels we observe the uncorrelated Poisson distribution. Thus it may be a very realistic system to prove that $\frac{1}{f^{\alpha}}$ noise is ubiquitous in nature

High monocytic MDSC signature predicts multi-drug resistance and cancer relapse in non-Hodgkin lymphoma patients treated with R-CHOP

IntroductionNon-Hodgkin Lymphoma (NHL) is a heterogeneous lymphoproliferative malignancy with B cell origin. Combinatorial treatment of rituximab, cyclophsphamide, hydroxydaunorubicin, oncovin, prednisone (R-CHOP) is the standard treatment regimen for NHL, yielding a complete remission (CR) rate of 40-50%. Unfortunately, considerable patients undergo relapse after CR or initial treatment, resulting in poor clinical implications. Patient’s response to chemotherapy varies widely from static disease to cancer recurrence and later is primarily associated with the development of multi-drug resistance (MDR). The immunosuppressive cells within the tumor microenvironment (TME) have become a crucial target for improving the therapy efficacy. However, a better understanding of their involvement is needed for distinctive response of NHL patients after receiving chemotherapy to design more effective front-line treatment algorithms based on reliable predictive biomarkers.MethodsPeripheral blood from 61 CD20+ NHL patients before and after chemotherapy was utilized for immunophenotyping by flow-cytometry at different phases of treatment. In-vivo and in-vitro doxorubicin (Dox) resistance models were developed with murine Dalton’s lymphoma and Jurkat/Raji cell-lines respectively and impact of responsible immune cells on generation of drug resistance was studied by RT-PCR, flow-cytometry and colorimetric assays. Gene silencing, ChIP and western blot were performed to explore the involved signaling pathways.ResultsWe observed a strong positive correlation between elevated level of CD33+CD11b+CD14+CD15- monocytic MDSCs (M-MDSC) and MDR in NHL relapse cohorts. We executed the role of M-MDSCs in fostering drug resistance phenomenon in doxorubicin-resistant cancer cells in both in-vitro, in-vivo models. Moreover, in-vitro supplementation of MDSCs in murine and human lymphoma culture augments early expression of MDR phenotypes than culture without MDSCs, correlated well with in-vitro drug efflux and tumor progression. We found that MDSC secreted cytokines IL-6, IL-10, IL-1β are the dominant factors elevating MDR expression in cancer cells, neutralization of MDSC secreted IL-6, IL-10, IL-1β reversed the MDR trait. Moreover, we identified MDSC secreted IL-6/IL-10/IL-1β induced STAT1/STAT3/NF-κβ signaling axis as a targeted cascade to promote early drug resistance in cancer cells.ConclusionOur data suggests that screening patients for high titre of M-MDSCs might be considered as a new potential biomarker and treatment modality in overcoming chemo-resistance in NHL patients

Molecular characterization and clinical relevance of metabolic expression subtypes in human cancers.

Metabolic reprogramming provides critical information for clinical oncology. Using molecular data of 9,125 patient samples from The Cancer Genome Atlas, we identified tumor subtypes in 33 cancer types based on mRNA expression patterns of seven major metabolic processes and assessed their clinical relevance. Our metabolic expression subtypes correlated extensively with clinical outcome: subtypes with upregulated carbohydrate, nucleotide, and vitamin/cofactor metabolism most consistently correlated with worse prognosis, whereas subtypes with upregulated lipid metabolism showed the opposite. Metabolic subtypes correlated with diverse somatic drivers but exhibited effects convergent on cancer hallmark pathways and were modulated by highly recurrent master regulators across cancer types. As a proof-of-concept example, we demonstrated that knockdown of SNAI1 or RUNX1—master regulators of carbohydrate metabolic subtypes-modulates metabolic activity and drug sensitivity. Our study provides a system-level view of metabolic heterogeneity within and across cancer types and identifies pathway cross-talk, suggesting related prognostic, therapeutic, and predictive utility

Separation of gold and silver using a chelating resin - Thiosemicarbazide incorporated Amberlite IRC-50

531-534A thiosemicarbazide group incorporated into a weakly acidic resin, Amberlite IRC-50 can selectively entrap two noble metals, gold and silver. The exchange capacity of the newly formed resin for Au(III) and Ag(I) is satisfactory. The resin can be utilized for the separation of these noble metals from different complex matrices

Investigation on pH dependent uptake of Cr(III) and Cr(VI) by Baker’s yeast

417-419Yeast cells of Saccharomyces cerevisiae were found to accumulate ⁵¹Cr(III) radioisotope at basic pH in trace level. There was no uptake of Cr(VI) at the same pH (~10.5). An assay of the products of cell lysis reveals that Cr(III) first gets adsorbed at the cell wall and then slowly enters the cytoplasm. On the other hand Cr(VI) has a faster penetration into the cytoplasm which increases with time, attains a maximum value and then release the metal ion from cytoplasm. The behavior and uptake kinetics of ⁵¹Cr(III) or ⁵¹Cr(VI) were studied using -spectrometry

Chaperone protein HYPK interacts with the first 17 amino acid region of Huntingtin and modulates mutant HTT-mediated aggregation and cytotoxicity

Huntington's disease is a polyglutamine expansion disorder, characterized by mutant HTT-mediated aggregate formation and cytotoxicity. Many reports suggests roles of N-terminal 17 amino acid domain of HTT (HTT-N17) towards subcellular localization, aggregate formation and subsequent pathogenicity induced by N-terminal HTT harboring polyQ stretch in pathogenic range. HYPK is a HTT-interacting chaperone which can reduce N-terminal mutant HTT-mediated aggregate formation and cytotoxicity in neuronal cell lines. However, how HYPK interacts with N-terminal fragment of HTT remained unknown. Here we report that specific interaction of HYPK with HTT-N17 is crucial for the chaperone activity of HYPK. Deletion of HTT-N17 leads to formation of tinier, SDS-soluble nuclear aggregates formed by N-terminal mutant HTT. The increased cytotoxicity imparted by these tiny aggregates might be contributed due to loss of interaction with HYPK. (C) 2014 Elsevier Inc. All rights reserved