24 research outputs found
Promocijas darbs
Get PDFElektroniskÄ versija nesatur pielikumusTrimetilamÄ«ns ir ticis identificÄts kÄ viens no baktÄriju metabolÄ«tiem ar potenciÄli negatÄ«vu ietekmi uz cilvÄka veselÄ«bu, kas padara to par aktuÄlu pÄtniecÄ«bas objektu. Å ajÄ darbÄ tika veikti bakteriÄlo trimetilamÄ«nu producÄjoÅ”o enzÄ«mu pÄtÄ«jumi. Tika veikta Klebsiella pneumoniae CutC holÄ«na liÄzes 3D kristÄlstruktÅ«ras noteikÅ”ana divÄs dažÄdÄs konformÄcijÄs. Tika pÄtÄ«ti arÄ« ar CutC holÄ«na liÄzi saistÄ«to GRM2 tipa bakteriÄlo mikrokompartmentu paÅ”salikÅ”anÄs un iepakoÅ”anas mehÄnismi. MÄs atklÄjÄm, ka serdes enzÄ«mi tiek iepakota hierarhiskÄ manierÄ, prezentÄjam krio-EM 3D struktÅ«ru 3.3 Ć
izŔķirtspÄjÄ un pÄtÄ«jÄm priekÅ”noteikumus rekobinantu Äaulu izveidoÅ”anai. MÄs arÄ« analizÄjÄm CntA oksigenÄzes substrÄtu paneļus no Äetriem dažÄdiem saimniekorganismiem, tÄdÄjÄdi atrisinot dažas iepriekÅ”Äjas neskaidrÄ«bas par Å”o enzÄ«mu.Trimethylamine has been identified as a bacterial metabolite with a potential negative impact on health, making it a target of interest. In this study the investigation of bacterial trimethylamine producing enzymes was performed. We solved the 3D crystal structures of two different conformations of CutC choline lyase from Klebsiella pneumoniae. The assembly and encapsulation mechanisms of the CutC associated GRM2 type bacterial microcompartments were also investigated. We discovered that the core enzymes are encapsulated in a hierachial manner, present a cryo-EM 3D structure of a pT=4 quasi symmetric shell particle at 3.3 Ć
resolution and studied the requirements for recombinant shell formation. We also analyzed the substrate panels of CntA oxygenase from four different hosts, thus clearing some previous controversies about this enzyme
System Dynamic Modelling of the Strategic Development of Agriculture
Get PDFIn the paper two main development models are shown: agriculture industry development model based on the estimations of experts (model of experts) and agriculture science development model. The experts of the Republic of Latvia Ministry of Agriculture take part in the preparation of the model data, and the results of the models are used in Latvia agriculture industry and science development strategy. The model results show that it is possible to reach profit per employee as the EU average. For this the number of employees have to diminish not less than two times. The level of profit for living in the countries lifts to average EU level. It is possible to develop the alternative agricultural production also. The above mentioned can be achieved only along with comprehensive developments of the countries: education, infrastructure, technologies, etc. Agriculture industry development is constrained to the potential of scientific research in the industry. Furthermore, the number of scientists is declining. Only increasing the investments in technologies by five times in comparison with the current level, the decline in the numbers of scientists in agriculture industry can be stopped.lauksaimniecÄ«bas ekonomika, stratÄÄ£ija, sistÄmdinamika, modelÄÅ”ana, ražība, sÄjumu platÄ«ba
LauksaimniecÄ«bas nozares stratÄÄ£iskÄs attÄ«stÄ«bas sistÄmdinamikas modelÄÅ”ana
Get PDFIn the paper two main development models are shown: agriculture industry development model based on the estimations of experts (model of experts) and agriculture science development model. The experts of the Republic of Latvia Ministry of Agriculture take part in the preparation of the model data, and the results of the models are used in Latvia agriculture industry and science development strategy. The model results show that it is possible to reach profit per employee as the EU average. For this the number of employees have to diminish not less than two times. The level of profit for living in the countries lifts to average EU level. It is possible to develop the alternative agricultural production also. The above mentioned can be achieved only along with comprehensive developments of the countries: education, infrastructure, technologies, etc. Agriculture industry development is constrained to the potential of scientific research in the industry. Furthermore, the number of scientists is declining. Only increasing the investments in technologies by five times in comparison with the current level, the decline in the numbers of scientists in agriculture industry can be stopped
LauksaimniecÄ«bas nozares stratÄÄ£iskÄs attÄ«stÄ«bas sistÄmdinamikas modelÄÅ”ana
Get PDFIn the paper two main development models are shown: agriculture industry development model based on the estimations of experts (model of experts) and agriculture science development model. The experts of the Republic of Latvia Ministry of Agriculture take part in the preparation of the model data, and the results of the models are used in Latvia agriculture industry and science development strategy. The model results show that it is possible to reach profit per employee as the EU average. For this the number of employees have to diminish not less than two times. The level of profit for living in the countries lifts to average EU level. It is possible to develop the alternative agricultural production also. The above mentioned can be achieved only along with comprehensive developments of the countries: education, infrastructure, technologies, etc. Agriculture industry development is constrained to the potential of scientific research in the industry. Furthermore, the number of scientists is declining. Only increasing the investments in technologies by five times in comparison with the current level, the decline in the numbers of scientists in agriculture industry can be stopped
Large-Scale Recombinant Production of the SARS-CoV-2 Proteome for High-Throughput and Structural Biology Applications
Get PDFThe highly infectious disease COVID-19 caused by the Betacoronavirus SARS-CoV-2 poses a severe threat to humanity and demands the redirection of scientific efforts and criteria to organized research projects. The international COVID19-NMR consortium seeks to provide such new approaches by gathering scientific expertise worldwide. In particular, making available viral proteins and RNAs will pave the way to understanding the SARS-CoV-2 molecular components in detail. The research in COVID19-NMR and the resources provided through the consortium are fully disclosed to accelerate access and exploitation. NMR investigations of the viral molecular components are designated to provide the essential basis for further work, including macromolecular interaction studies and high-throughput drug screening. Here, we present the extensive catalog of a holistic SARS-CoV-2 protein preparation approach based on the consortiumās collective efforts. We provide protocols for the large-scale production of more than 80% of all SARS-CoV-2 proteins or essential parts of them. Several of the proteins were produced in more than one laboratory, demonstrating the high interoperability between NMR groups worldwide. For the majority of proteins, we can produce isotope-labeled samples of HSQC-grade. Together with several NMR chemical shift assignments made publicly available on covid19-nmr.com, we here provide highly valuable resources for the production of SARS-CoV-2 proteins in isotope-labeled form
Delivery of virus-like particles to eucariotic cells
No full textÅ Ä« darba mÄrÄ·is bija pÄtÄ«t vÄ«rusiem lÄ«dzÄ«go daļiÅu (VLD) piegÄdÄÅ”anas iespÄjas uz ŔūnÄm, izmantojot preS1 sekvenci un tÄs fragmentus. Otrs uzdevums bija Latvijas OrganiskÄ SintÄzes InstitÅ«tÄ (LOSI) sintezÄtu transfekcijas lÄ«dzekļu izpÄte proteÄ«nu un to kompleksu virzÄ«Å”anai uz ŔūnÄm. VarÄja secinÄt, ka preS1 sekvences adresÄjoÅ”ais efekts uz HepG2 ŔūnÄm nav liels. Pilna garuma preS1 sekvencei piemita spÄja nodroÅ”inÄt VLD uzÅemÅ”anu atseviŔķÄs HepG2 un Hek 293 ŔūnÄs, kamÄr preS1 sekvences imunodominantais 10-36 aminoskÄbju fragments to nenodroÅ”inÄja pie tiem paÅ”iem apstÄkļiem. ProteÄ«nu ransfekcijas lÄ«dzekļu efektivitÄte tika raksturota kvantitatÄ«vi un kvalitatÄ«vi salÄ«dzinÄjumÄ ar komerciÄliem lÄ«dzekļiem. TÄlÄkai pÄtÄ«Å”anai varÄja tikt izvirzÄ«ti vairÄki transfekcijas lÄ«dzekļi, kas bija efektÄ«vi salÄ«dzinÄjumÄ ar komerciÄlajiem lÄ«dzekļiem. AtslÄgas vÄrdi: bakteriofÄgs AP 205, hepatÄ«ta B vÄ«russ, bakteriofÄgs GA, katjoniskie lipÄ«di, vÄ«rusiem lÄ«dzÄ«gÄs daļiÅas, preS1 sekvence, piegÄde uz ŔūnÄm.One of the aims of this work was to study delivery of virus-like particles (VLP) to cells using HBV preS1 sequence. Second aim of this work was study in LOSI produced transfection reagent effectiveness in delivering proteins and protein complexes to eukaryotic cells. It was concluded that preS1 sequence is not effective in addresing virus-like particles to HepG2 cells. Full length preS1 sequence mediates virus-like particle uptake in few HepG2 and Hek293 cells, while immunodomnant 10-36 amino acid fragment could not in the same experimental conditions. Protein transfection was studied using both quantitative and qualitative methods, and compared to commercial transfection reagents. For further study could be distinguished several transfection reagents with activity equal or above in comparision to commercial transfection reagents. Key words: bacteriophage AP205, hepatitus B virus, bacteriophage GA, virus-like particles, preS1 sequence, cationic lipids, delivery to cells
Interactions of targeted virus like particles with eucariotic calls
No full textÅ Ä« darba mÄrÄ·is bija pÄtÄ«t ar preS1 sekvences fragmentiem dekorÄto AP 205 bakteriofÄga apvalka proteÄ«na un hepatÄ«ta B vÄ«rusa core proteÄ«na veidoto vÄ«rusiem lÄ«dzÄ«go daļiÅu (VLD) mijiedarbÄ«bu ar dažÄdu eikariotu Ŕūnu lÄ«nijÄm āHepG2, Hek293, Jurkat, Namalva un BHK.
Tika izstrÄdÄtas metodes VLD iezÄ«mÄÅ”anai ar fluorescento iezÄ«mi un to saistÄ«bas pÄtÄ«Å”anai ar eikariotu ŔūnÄm, izmantojot fluorimetrijas, Ŕūnu citometrijas, fluorescences mikroskopijas un lÄzerskenÄjoÅ”Äs konfokÄlÄs mikroskopijas metodes. VarÄja secinÄt, ka no HBV core proteÄ«na atvasinÄto VLD mijiedarbÄ«bas rezultÄtu ietekmÄ DNS saistoÅ”ais domÄns. SavukÄrt uz VLD virsmas eksponÄti preS1 sekvences fragmenti pÄtÄ«tajÄs konstrukcijÄs nenodroÅ”ina pÄrliecinoÅ”i augstÄku mijiedarbÄ«bu ar ŔūnÄm, taÄu visgarÄkÄ preS1 fragmenta klÄtbÅ«tne VLD sastÄvÄ nodroÅ”inÄja daļiÅu internalizÄciju ŔūnÄs.
Å is darbs tika izstrÄdÄts Latvijas BiomedicÄ«nas petÄ«jumu un studiju centrÄ laikÄ no 2010. gada septembra lÄ«dz 2011. gada maijam.
AtslÄgas vÄrdi: bakteriofÄgs AP 205, hepatÄ«ta B vÄ«russ, vÄ«rusiem lÄ«dzÄ«gÄs daļiÅas, preS1 sekvence, piegÄdÄÅ”ana uz ŔūnÄm.The aim of this work was to study AP205 bacteriophage coat protein and hepatitis B virus core protein virus-like particles, decorated with preS1 sequence fragments, interaction with different eukaryotic cell lines: HepG2, Hek293, Jurkat, Namalva and BHK.
There were developed methods for labeling virus-like particles with FITC label and studying their interaction with eukaryotic cell lines, using fluorimetry, cell cytometry, fluorescent microscopy, confocal laser-scanning microscopy and immunofluorescence methods. It was concluded that HBV core protein DNA-binding domain is essential for VLP interaction with cells. It was also concluded that decorating AP 205 phage coat protein VLP with preS1 sequence fragments does not statistically significantly improve the ability of VLP to interact with cells, but the presence of the longest preS1 sequence fragment mediate VLP internalisation into cells.
The work was performed in Latvian Biomedical Research and Study Centre from September 2010 to May 2011.
Key words: bacteriophage AP205, virus-like particles, preS1 sequence, delivery to cells
Konstrukciju uzvedÄ«bas metamodeļa izvÄle, izmantojot heiristisku stÄvokļu telpas pÄrmeklÄÅ”anu
No full textMetamodes in Design of GFRP Composite Stiffened Deck Structure
No full textThis paper deals with the comparison of parametric and non-parametric metamodels
used in design procedure of pultruded glass fibre reinforced plastic (GFRP) deck panels.
Stiffened deck structure loaded under the three-point bending and uniform distributed load
has been examined. A set of five hundred sample points has been evaluated by means of
finite element analysis and stiffness and strength responses extracted for building of
metamodels. Moreover, set of complimentary physical experiments has been carried out in
order to verify the numerical model. Both advantages and disadvantages of parametric and
non-parametric metamodels have been drawn for a specific engineering application
