22 research outputs found
Is the porcine RN locus a pleiotropic QTL? A Bayesian marker assisted segregation analysis
International audienc
Genomic scan for quantitative trait loci of chemical and physical body composition and deposition on pig chromosome X including the pseudoautosomal region of males
A QTL analysis of pig chromosome X (SSCX) was carried out using an approach that accurately takes into account the specific features of sex chromosomes i.e. their heterogeneity, the presence of a pseudoautosomal region and the dosage compensation phenomenon. A three-generation full-sib population of 386 animals was created by crossing Pietrain sires with a crossbred dam line. Phenotypic data on 72 traits were recorded for at least 292 and up to 315 F2 animals including chemical body composition measured on live animals at five target weights ranging from 30 to 140 kg, daily gain and feed intake measured throughout growth, and carcass characteristics obtained at slaughter weight (140 kg). Several significant and suggestive QTL were detected on pig chromosome X: (1) in the pseudoautosomal region of SSCX, a QTL for entire loin weight, which showed paternal imprinting, (2) closely linked to marker SW2456, a suggestive QTL for feed intake at which Pietrain alleles were found to be associated with higher feed intake, which is unexpected for a breed known for its low feed intake capacity, (3) at the telomeric end of the q arm of SSCX, QTL for jowl weight and lipid accretion and (4) suggestive QTL for chemical body composition at 30 kg. These results indicate that SSCX is important for physical and chemical body composition and accretion as well as feed intake regulation
Estimation of breed contributions to present and future genetic diversity of 44 North Eurasian cattle breeds using core set diversity measures
v2006o
Estimation of breed contributions to present and future genetic diversity of 44 North Eurasian cattle breeds using core set diversity measures
Extinction of breeds threatens genetic diversity of livestock species. The
need to conserve genetic diversity is widely accepted but involves in
general two questions: (i) is the expected loss of diversity in a set of
breeds within a defined future time horizon large enough to establish a
conservation plan, and if so (ii) which breeds should be prioritised for
such a conservation plan? The present study uses a marker assisted
methodology to address these questions. The methodology combines core set
diversity measures with a stochastic method for the estimation of expected
future diversity and breed marginal diversities. The latter is defined as
the change in the total diversity of all breeds caused by a one unit
decrease in extinction probability of a particular breed. The stochastic
method was validated by means of simulations. A large field data set
consisting of 44 North Eurasian cattle breeds was analysed using simplified
determined extinction probabilities. The results show that the expected loss
of diversity in this set within the next 20 to 50 years is between 1 and
3% of the actual diversity, provided that
the extinction probabilities
which were used are approximately valid. If this loss is to be reduced, it
is sufficient to include those three to five breeds with the highest
marginal diversity in a conservation scheme
A high-density linkage map of the RN region in pigs
The porcine RN locus affects muscle glycogen content and meat quality. We previously mapped the RN locus to chromosome 15. This study describes the identification of polymorphisms for four class I and four class II markers located in the RN region. Resource families were genotyped with F-SSCP markers (fluorescent single strand conformation polymorphism) and microsatellite markers. Subsequent multipoint linkage analysis revealed the order FN1-IGFBP5-S1000-S1001-IL8RB-VIL1-RN-Sw936-Sw906. The gene order is identical to the previously reported porcine RH map of the same region. The described map will facilitate positional cloning of the RN gene
Combined analysis of data from two granddaughter designs: A simple strategy for QTL confirmation and increasing experimental power in dairy cattle
A joint analysis of five paternal half-sib Holstein families that were part of two different granddaughter designs (ADR- or Inra-design) was carried out for five milk production traits and somatic cell score in order to conduct a QTL confirmation study and to increase the experimental power. Data were exchanged in a coded and standardised form. The combined data set (JOINT-design) consisted of on average 231 sires per grandsire. Genetic maps were calculated for 133 markers distributed over nine chromosomes. QTL analyses were performed separately for each design and each trait. The results revealed QTL for milk production on chromosome 14, for milk yield on chromosome 5, and for fat content on chromosome 19 in both the ADR- and the Inra-design (confirmed within this study). Some QTL could only be mapped in either the ADR- or in the Inra-design (not confirmed within this study). Additional QTL previously undetected in the single designs were mapped in the JOINT-design for fat yield (chromosome 19 and 26), protein yield (chromosome 26), protein content (chromosome 5), and somatic cell score (chromosome 2 and 19) with genomewide significance. This study demonstrated the potential benefits of a combined analysis of data from different granddaughter designs