PINSPOT: An oPen platform for INtelligent context-baSed Indoor POsiTioning

This work proposes PINSPOT; an open-access platform for collecting and sharing of context, algorithms and results in the cutting-edge area of indoor positioning. It is envisioned that this framework will become reference point for knowledge exchange which will bring the research community even closer and potentially enhance collaboration towards more effective and efficient creation of indoor positioning-related knowledge and innovation. Specifically, this platform facilitates the collection of sensor data useful for indoor positioning experimentation, the development of novel, self-learning, indoor positioning algorithms, as well as the enhancement and testing of existing ones and the dissemination and sharing of the proposed algorithms along with their configuration, the data used, and with their results

Gata2 in Embryonic Hematopoiesis

Hematopoiesis is a word originating from the two greek words αἷμα (haima) which means blood and the verb ποιεῖν (poien) which means to make/create. Hematopoiesis describes the process by which the organism creates and replenishes all the blood cell types that are required for the physiologic functions of the organism. The importance of the blood tissue can be highlighted by the many and discrete functions that it performs. These are accomplished through several different cell types forming the blood tissue (erythrocytes, platelets, macrophages, neutrophils, eosinophils, basophils, B-cells, T-cells, NK-cells). For example, the red blood cells or erythrocytes found in the circulating blood are mainly involved in the transport of O2 and CO2. Lymphocytes which are white blood cells are part of the immune system and actively participate in the defense of the organism against pathogens. In the adult organism hematopoietic cells are found not only in the blood but also in hematopoietic tissues such as the bone marrow, spleen, lymph nodes and thymus. Importantly, all mature hematopoietic cell types found in the blood tissues originate from rare hematopoietic stem cells (HSCs). These founder cells are quiescent, long-lived and are at the base of a well-studied cell differentiation hierarchy. HSCs robustly produce all the billions of mature blood cells that are required daily and throughout the entire life of the organism. HSCs are clinically relevant cells that have been used for over 50 years in transplantation and cell replacement therapies for leukemia and monogenic blood-related diseases

An Experience Report on the Effectiveness of Five Themed Workshops at Inspiring High School Students to Learn Coding

Today there is a high demand for computing programmers, and at the same time a shortage of skilled professionals. This has triggered the creation of many initiatives in the past few years, with the aim of reversing the phenomenon. To achieve this, such events are designed to promote a more appealing image for programming, both as a profession and as a skill. This paper describes one such initiative, which uses a unique blend of differently themed, parallel workshops to motivate high school students to learn programming. With the use of questionnaires, we survey the participants and present our findings concerning the effectiveness of these workshops to engage the participants, to promote the value of coding, and to encourage the participants to consider a career in the field. We evaluate our results both at a general level, as well as by comparison among five individually themed workshops

FINDbase: a relational database recording frequencies of genetic defects leading to inherited disorders worldwide

Frequency of INherited Disorders database (FINDbase) () is a relational database, derived from the ETHNOS software, recording frequencies of causative mutations leading to inherited disorders worldwide. Database records include the population and ethnic group, the disorder name and the related gene, accompanied by links to any corresponding locus-specific mutation database, to the respective Online Mendelian Inheritance in Man entries and the mutation together with its frequency in that population. The initial information is derived from the published literature, locus-specific databases and genetic disease consortia. FINDbase offers a user-friendly query interface, providing instant access to the list and frequencies of the different mutations. Query outputs can be either in a table or graphical format, accompanied by reference(s) on the data source. Registered users from three different groups, namely administrator, national coordinator and curator, are responsible for database curation and/or data entry/correction online via a password-protected interface. Databaseaccess is free of charge and there are no registration requirements for data querying. FINDbase provides a simple, web-based system for population-based mutation data collection and retrieval and can serve not only as a valuable online tool for molecular genetic testing of inherited disorders but also as a non-profit model for sustainable database funding, in the form of a ‘database-journal’

Business model analysis of low-cost carriers on the exaple of Ryanair

nicht vorhande

Human Gain-of-Function MC4R Variants Show Signaling Bias and Protect against Obesity.

The melanocortin 4 receptor (MC4R) is a G protein-coupled receptor whose disruption causes obesity. We functionally characterized 61 MC4R variants identified in 0.5 million people from UK Biobank and examined their associations with body mass index (BMI) and obesity-related cardiometabolic diseases. We found that the maximal efficacy of β-arrestin recruitment to MC4R, rather than canonical Gαs-mediated cyclic adenosine-monophosphate production, explained 88% of the variance in the association of MC4R variants with BMI. While most MC4R variants caused loss of function, a subset caused gain of function; these variants were associated with significantly lower BMI and lower odds of obesity, type 2 diabetes, and coronary artery disease. Protective associations were driven by MC4R variants exhibiting signaling bias toward β-arrestin recruitment and increased mitogen-activated protein kinase pathway activation. Harnessing β-arrestin-biased MC4R signaling may represent an effective strategy for weight loss and the treatment of obesity-related cardiometabolic diseases

In vivo single cell analysis reveals Gata2 dynamics in cells transitioning to hematopoietic fate

Cell fate is established through coordinated gene expression programs in individual cells. Regulatory networks that include the Gata2 transcription factor play central roles in hematopoietic fate establishment. Although Gata2 is essential to the embryonic development and function of hematopoietic stem cells that form the adult hierarchy, little is known about the in vivo expression dynamics of Gata2 in single cells. Here, we examine Gata2 expression in single aortic cells as they establish hematopoietic fate in Gata2Venus mouse embryos. Time-lapse imaging reveals rapid pulsatile level changes in Gata2 reporter expression in cells undergoing endothelial-to-hematopoietic transition. Moreover, Gata2 reporter pulsatile expression is dramatically altered in Gata2+/- aortic cells, which undergo fewer transitions and are reduced in hematopoietic potential. Our novel finding of dynamic pulsatile expression of Gata2 suggests a highly unstable genetic state in single cells concomitant with their transition to hematopoietic fate. This reinforces the notion that threshold levels of Gata2 influence fate establishment and has implications for transcription factor-related hematologic dysfunctions