Gestaltung von Rückzugsorten: Gestaltungsmöglichkeiten dezentraler Rückzugsorte für Pflegepersonal in Notaufnahmen

Das Pflegepersonal in Notaufnahmen ist vielen Stresssituationen, sowie physischen und psychischen Gefahren ausgesetzt. Arbeitspausen entlasten das Personal. Um die Pausen so angenehm wie möglich zu gestalten, sollten die Bedürfnisse des Personals berücksichtigt werden. Ruhe während der Pause ist wichtig für Aspekte wie Aufmerksamkeit, Gesundheit und Leistung. In einem Aufenthaltsraum einer Notaufnahme ist Ruhe nicht immer gewährleistet. Dezentrale Rückzugsorte, für den individuellen Rückzug für das Pflegepersonal, sind nicht vorhanden. Die vorliegende Arbeit untersucht den Bedarf, Anordnung und Gestaltung von dezentralen Rückzugsorten für das Pflegepersonal einer Notaufnahme. Um die Wünsche und Bedürfnisse des Pflegepersonals in den Gestaltungsprozess mit einzubeziehen, wurde eine Umfrage zur aktuellen Pausengestaltung und den Bedürfnissen durchgeführt. Laut der Umfrage ist dem Pflegepersonal das Gefühl von Ruhe am wichtigsten. Dieses kann durch abseits vom Stress gelegene Rückzugsorte gewährleistet werden. Auf dieser Grundlage ist es empfehlenswert, dezentrale Rückzugsorte innerhalb der Notaufnahme mit Abstand zu Behandlungsräumen und zum Stützpunkt zu planen, um das Bedürfnis nach Ruhe zu stillen. Zudem wurden Gestaltungselemente für einen Rückzugsort entwickelt, die auf Grundlage der erforschten Erkenntnisse durch Literaturrecherche und die Umfrage entstanden sind

Wide-range monitor for pulsed x-ray sources

A monitoring instrument based on a high-pressure ionization chamber has been developed that measures average dose rates as low as 0.1 mR/h and responds linearly to short pulses at dose rates up to 1.2 x 10/sup 10/ R/h. Its sensitivity can be remotely changed by a factor of 10/sup 4/, to enable accurate measurement of both background radiation and very high intensities such as can be expected from accelerator beam-spills. The instrument's detector-electrometer pulse response was measured using a dose-calibrated field-emission accelerator having a 30-ns pulse width

Comparison of independent screens on differentially vulnerable motor neurons reveals alpha-synuclein as a common modifier in motor neuron diseases

The term "motor neuron disease" encompasses a spectrum of disorders in which motor neurons are the primary pathological target. However, in both patients and animal models of these diseases, not all motor neurons are equally vulnerable, in that while some motor neurons are lost very early in disease, others remain comparatively intact, even at late stages. This creates a valuable system to investigate the factors that regulate motor neuron vulnerability. In this study, we aim to use this experimental paradigm to identify potential transcriptional modifiers. We have compared the transcriptome of motor neurons from healthy wild-type mice, which are differentially vulnerable in the childhood motor neuron disease Spinal Muscular Atrophy (SMA), and have identified 910 transcriptional changes. We have compared this data set with published microarray data sets on other differentially vulnerable motor neurons. These neurons were differentially vulnerable in the adult onset motor neuron disease Amyotrophic Lateral Sclerosis (ALS), but the screen was performed on the equivalent population of neurons from neurologically normal human, rat and mouse. This cross species comparison has generated a refined list of differentially expressed genes, including CELF5, Col5a2, PGEMN1, SNCA, Stmn1 and HOXa5, alongside a further enrichment for synaptic and axonal transcripts. As an in vivo validation, we demonstrate that the manipulation of a significant number of these transcripts can modify the neurodegenerative phenotype observed in a Drosophila line carrying an ALS causing mutation. Finally, we demonstrate that vector-mediated expression of alpha-synuclein (SNCA), a transcript decreased in selectively vulnerable motor neurons in all four screens, can extend life span, increase weight and decrease neuromuscular junction pathology in a mouse model of SMA. In summary, we have combined multiple data sets to identify transcripts, which are strong candidates for being phenotypic modifiers, and demonstrated SNCA is a modifier of pathology in motor neuron disease

Palladium-templated subcomponent self-assembly of macrocycles, catenanes, and rotaxanes.

The reaction of 2,6-diformylpyridine with diverse amines and Pd(II) ions gave rise to a variety of metallosupramolecular species, in which the Pd(II) ion is observed to template a tridentate bis(imino)pyridine ligand. These species included a mononuclear complex as well as [2+2] and [3+3] macrocycles. The addition of pyridine-containing macrocyclic capping ligands allows for topological complexity to arise, thereby enabling the straightforward preparation of structures that include a [2]catenane, a [2]rotaxane, and a doubly threaded [3]rotaxane.This work was underwritten by the Marie Curie Academic-Industrial Initial Training Network on Dynamic Molecular Nanostructures (DYNAMOL) of the European Union’s Seventh Framework Programme (FP7) and the UK Engineering and Physical Sciences Research Council (EPSRC). We thank the Cambridge Chemistry NMR service for experimental assistance, Diamond Light Source (UK) for synchrotron beamtime on I19 (MT7984), and the EPSRC National Crystallography Service for X-ray data collection.This is the final version of the article. It first appeared from Wiley via http://dx.doi.org/10.1002/anie.20140616

Ionicity-dependent proton-coupled electron transfer of supramolecular self-assembled electroactive heterocycles

Herein, we investigate the electrochemical properties of a class of Supramolecular Self-associated Amphiphilic salts (SSAs). We show that varying ionic strength of an SSA solution can cause a switching of the thermodynamics and kinetics of electron transfer. The effect of self-assembly on proton-coupled electron transfer has implications for the understanding of electron transfer kinetics in aqueous organic redox flow batteries, especially at high concentration where organic–organic intermolecular interactions become dominant even for highly soluble organic species

Self-oligomerization Regulates Stability of Survival Motor Neuron Protein Isoforms by Sequestering an SCF\u3csup\u3eSlmb\u3c/sup\u3e Degron

Spinal muscular atrophy (SMA) is caused by homozygous mutations in human SMN1. Expression of a duplicate gene (SMN2) primarily results in skipping of exon 7 and production of an unstable protein isoform, SMNΔ7. Although SMN2 exon skipping is the principal contributor to SMA severity, mechanisms governing stability of survival motor neuron (SMN) isoforms are poorly understood. We used a Drosophila model system and label-free proteomics to identify the SCFSlmb ubiquitin E3 ligase complex as a novel SMN binding partner. SCFSlmb interacts with a phosphor degron embedded within the human and fruitfly SMN YG-box oligomerization domains. Substitution of a conserved serine (S270A) interferes with SCFSlmb binding and stabilizes SMNΔ7. SMA-causing missense mutations that block multimerization of full-length SMN are also stabilized in the degron mutant background. Overexpression of SMNΔ7S270A, but not wild-type (WT) SMNΔ7, provides a protective effect in SMA model mice and human motor neuron cell culture systems. Our findings support a model wherein the degron is exposed when SMN is monomeric and sequestered when SMN forms higher-order multimers

Voltammetric Characterization of Redox-Inactive Guest Binding to Ln III [15-Metallacrown-5] Hosts Based on Competition with a Redox Probe

A novel competitive binding assay was implemented to monitor the binding of a redox inactive substrate to a redox inactive metallacrown host based on its competition with ferrocene carboxylate (FcC − ) using cyclic voltammetry (CV). First, the binding of FcC − to Ln III [15-MC Cu II ,N,L-pheHA -5] (LnMC) hosts was characterized by cyclic voltammetry. It was shown that the voltammetric half wave potentials, E 1/2 , shifted to more positive potentials upon the addition of LnMC. The explicit dependence of E 1/2 with the concentration of LnMC was used to determine the association constants for the complex. The FcC − binding strength decreased with larger central lanthanide metals in the LnMC hosts, and substantially weaker binding was observed with La III . X-ray crystallography revealed that the hydrophobic host cavity incompletely encapsulated FcC − when the guest was bound to the nine-coordinate La III , suggesting the LnMC’s ligand side chains play a substantial role in guest recognition. With knowledge of the MC-FcC − solution thermodynamics, the binding affinity of a redox inactive guest was then assessed. Addition of sodium benzoate to a LnMC and FcC − mixture resulted in E 1/2 shifting back to the value observed for FcC − in the absence of LnMC. The association constants between benzoate and LnMC’s were calculated via the competitive binding approach. Comparison with literature values suggests this novel assay is a viable method for determining association constants for host–guest systems that exhibit the proper electrochemical behavior. Notably, this CV competitive binding approach does not require the preparation of a modified electrode or a tethered guest, and thus can be generalized to a number of host–guest systems.Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/77442/1/chem_200903015_sm_miscellaneous_information.pd