Analysis of solutions dedicated to non-conformity prevention

Research financed from the NCN research project no. UMO-2012/05 / B / HS4 / 04139.Purpose: The purpose of the work is to analyze the use of solutions aimed at preventing incompatibilities and assess the effectiveness of these solutions to minimize the risk associated with the quality of manufactured products. The analysis presented in the work is based on the Poka-Yoke error prevention concept used in the selected enterprise. The paper presents the state of the company before and after the introduction of Poka-Yoke method. Approach/Methodology/Design: The research methods used in the paper were the analysis of company data, analysis of literature states, as well as methods using the Poka-Yoke concept. The company was surveyed in 2017/2018 during the system implementation. Findings: The results on the control card for nonconforming units of type "p" were also presented, where the analysis showed that the introduction of the Poka-Yoke system to the company reduced the number of nonconformities by 6.69%. Practical Implications: Implementation of the Poka-Yoke system will allow enterprises to create conditions in which an error cannot happen, or will be immediately visible. The result of such an action may be distracting operators from repetitive operations, reducing the number of deficiencies and induce immediate action when a problem occurs. Originality/Value: Showing how the use of the Poka-Yoke system affects the prevention of inadvertent error in enterprises, by determining the differences between the state of the enterprise before and after the introduction of the Poka-Yoke system, there is an opportunity to improve the economy and contribute to the social and economic sphere.peer-reviewe

Supervisory control and data acquisition system as a means of safety at work formation in a manufacturing company

Purpose: The aim of the paper is to present an application of the Supervisory Control and Data Acquisition system (SCADA) in the shaping of occupational safety by raising workers’ awareness concerning importance of maintaining high personal safety standards in a manufacturing company. Approach/Methodology/Design: Shaping work safety covers all activities that increase employee awareness concerning compliance with safety regulations and work practices with particular respect to the rules of conduct in specific situations. This article presents the use of the SCADA system in shaping of the workplace safety. It also underlines its influence on raising employee awareness of the importance of complying with health and safety regulations. Findings: It can be stated that the safety days screen meets the company’s expectations. Moreover, it can be a valuable support in shaping workplace safety and raising employee awareness of safe work. The crucial thing is to carry out a detailed analysis and then choose such a solution that is adequate for the needs of a particular enterprise. Practical Implications: It is noted that the use of any solution that optimizes processes in the enterprise (including manufacturing processes) and the opportunities that IT tools provide, greatly contributes to the growth of safety. Providing safe working conditions by employers is usually closely linked to the organization's management strategy. Originality/Value: Currently we can observe the growing use of distributed control and visualization systems (SCADA systems) in the automation of industrial processes.peer-reviewe

mRNA adenosine methylase (MTA) deposits m6A on pri-miRNAs to modulate miRNA biogenesis in Arabidopsis thaliana

Copyright © 2020 the Author(s). Published by PNAS. In Arabidopsis thaliana, the METTL3 homolog, mRNA adenosine methylase (MTA) introduces N6-methyladenosine (m6A) into various coding and noncoding RNAs of the plant transcriptome. Here, we show that an MTA-deficient mutant (mta) has decreased levels of microRNAs (miRNAs) but accumulates primary miRNA transcripts (pri-miRNAs). Moreover, pri-miRNAs are methylated by MTA, and RNA structure probing analysis reveals a decrease in secondary structure within stem-loop regions of these transcripts in mta mutant plants. We demonstrate interaction between MTA and both RNA Polymerase II and TOUGH (TGH), a plant protein needed for early steps of miRNA biogenesis. Both MTA and TGH are necessary for efficient colocalization of the Microprocessor components Dicer-like 1 (DCL1) and Hyponastic Leaves 1 (HYL1) with RNA Polymerase II. We propose that secondary structure of miRNA precursors induced by their MTA-dependent m6A methylation status, together with direct interactions between MTA and TGH, influence the recruitment of Microprocessor to plant pri-miRNAs. Therefore, the lack of MTA in mta mutant plants disturbs pri-miRNA processing and leads to the decrease in miRNA accumulation. Furthermore, our findings reveal that reduced miR393b levels likely contributes to the impaired auxin response phenotypes of mta mutant plants

The SERRATE protein is involved in alternative splicing in <em>Arabidopsis thaliana</em>

Howalternative splicing (AS) is regulated in plants has not yet been elucidated. Previously, we have shown that the nuclear cap-binding protein complex (AtCBC) is involved in AS in Arabidopsis thaliana. Here we show that both subunits of AtCBC (AtCBP20 and AtCBP80) interact with SERRATE (AtSE), a protein involved in the microRNA biogenesis pathway. Moreover, using a high-resolution reverse transcript-ase-polymerase chain reaction AS system we have found that AtSE influences AS in a similar way to the cap-binding complex (CBC), preferentially affecting selection of 50 splice site of first introns. The AtSE protein acts in cooperation with AtCBC: many changes observed in the mutant lacking the correct SERRATE activity were common to those observed in the cbp mutants. Interestingly, significant changes in AS of some genes were also observed in other mutants of plant microRNA biogenesis pathway, hyl1-2 and dcl1-7, but a majority of them did not cor-respond to the changes observed in the se-1mutant. Thus, the role of SERRATE in AS regulation is distinct from that of HYL1andDCL1, and is similar to the regu-lation of AS in which CBC is involved

siRNAs: Potential therapeutic agents against Hepatitis C Virus

Hepatitis C virus is a major cause of chronic liver diseases which can lead to permanent liver damage, hepatocellular carcinoma and death. The presently available treatment with interferon plus ribavirin, has limited benefits due to adverse side effects such as anemia, depression and "flu-like" symptoms. Needless to mention, the effectiveness of interferon therapy is predominantly, if not exclusively, limited to virus type 3a and 3b whereas in Europe and North America the majority of viral type is 1a and 2a. Due to the limited efficiency of current therapy, RNA interference (RNAi) a novel regulatory and powerful silencing approach for molecular therapeutics through a sequence-specific RNA degradation process represents an alternative option. Several reports have indicated the efficiency and specificity of synthetic and vector based siRNAs inhibiting HCV replication. In the present review, we focused that combination of siRNAs against virus and host genes will be a better option to treat HC

Plasmid pP62BP1 isolated from an Arctic Psychrobacter sp. strain carries two highly homologous type II restriction-modification systems and a putative organic sulfate metabolism operon

The complete nucleotide sequence of plasmid pP62BP1 (34,467 bp), isolated from Arctic Psychrobacter sp. DAB_AL62B, was determined and annotated. The conserved plasmid backbone is composed of several genetic modules, including a replication system (REP) with similarities to the REP region of the iteron-containing plasmid pPS10 of Pseudomonas syringae. The additional genetic load of pP62BP1 includes two highly related type II restriction-modification systems and a set of genes (slfRCHSL) encoding enzymes engaged in the metabolism of organic sulfates, plus a putative transcriptional regulator (SlfR) of the AraC family. The pP62BP1 slflocus has a compact and unique structure. It is predicted that the enzymes SlfC, SlfH, SlfS and SlfL carry out a chain of reactions leading to the transformation of alkyl sulfates into acyl-CoA, with dodecyl sulfate (SDS) as a possible starting substrate. Comparative analysis of the nucleotide sequences of pP62BP1 and other Psychrobacter spp. plasmids revealed their structural diversity. However, the presence of a few highly conserved DNA segments in pP62BP1, plasmid 1 of P. cryohalolentis K5 and pRWF-101 of Psychrobacter sp. PRwf-1 is indicative of recombinational shuffling of genetic information, and is evidence of lateral gene transfer in the Arctic environment