Guaranteed discovery of the NmSuGra model

We analyze the discovery potential of the next-to-minimal supergravity motivated model: NmSuGra. This model is an extension of mSuGra by a gauge singlet, and contains only one additional parameter: lambda, the Higgs-singlet-Higgs coupling. NmSuGra solves the mu-problem and reduces the fine tuning of mSuGra. After identifying parameter space regions preferred by present experimental data, we show that these regions of NmSuGra are amenable to detection by the combination of the Large Hadron Collider and an upgraded Cryogenic Dark Matter Search. This conclusion holds strictly provided that the more than three sigma discrepancy in the difference of the experimental and the standard theoretical values of the anomalous magnetic moment of the muon prevails in the future.Comment: 3 pages, 3 figures, proceedings for SUSY08 and DSU0

Application of fracture mechanics and half-cycle method to the prediction of fatigue life of B-52 aircraft pylon components

Stress intensity levels at various parts of the NASA B-52 carrier aircraft pylon were examined for the case when the pylon store was the space shuttle solid rocket booster drop test vehicle. Eight critical stress points were selected for the pylon fatigue analysis. Using fracture mechanics and the half-cycle theory (directly or indirectly) for the calculations of fatigue-crack growth ,the remaining fatigue life (number of flights left) was estimated for each critical part. It was found that the two rear hooks had relatively short fatigue life and that the front hook had the shortest fatigue life of all the parts analyzed. The rest of the pylon parts were found to be noncritical because of their extremely long fatigue life associated with the low operational stress levels

Berberine Reduces cAMP-Induced Chloride Secretion in T84 Human Colonic Carcinoma Cells through Inhibition of Basolateral KCNQ1 Channels

Berberine is a plant alkaloid with multiple pharmacological actions, including antidiarrhoeal activity and has been shown to inhibit Cl− secretion in distal colon. The aims of this study were to determine the molecular signaling mechanisms of action of berberine on Cl− secretion and the ion transporter targets. Monolayers of T84 human colonic carcinoma cells grown in permeable supports were placed in Ussing chambers and short-circuit current measured in response to secretagogues and berberine. Whole-cell current recordings were performed in T84 cells using the patch-clamp technique. Berberine decreased forskolin-induced short-circuit current in a concentration-dependent manner (IC50 80 ± 8 μM). In apically permeabilized monolayers and whole-cell current recordings, berberine inhibited a cAMP-dependent and chromanol 293B-sensitive basolateral membrane K+ current by 88%, suggesting inhibition of KCNQ1 K+ channels. Berberine did not affect either apical Cl− conductance or basolateral Na+–K+-ATPase activity. Berberine stimulated p38 MAPK, PKCα and PKA, but had no effect on p42/p44 MAPK and PKCδ. However, berberine pre-treatment prevented stimulation of p42/p44 MAPK by epidermal growth factor. The inhibitory effect of berberine on Cl− secretion was partially blocked by HBDDE (∼65%), an inhibitor of PKCα and to a smaller extent by inhibition of p38 MAPK with SB202190 (∼15%). Berberine treatment induced an increase in association between PKCα and PKA with KCNQ1 and produced phosphorylation of the channel. We conclude that berberine exerts its inhibitory effect on colonic Cl− secretion through inhibition of basolateral KCNQ1 channels responsible for K+ recycling via a PKCα-dependent pathway

Transcript copy number estimation using a mouse whole-genome oligonucleotide microarray

The ability to quantitatively measure the expression of all genes in a given tissue or cell with a single assay is an exciting promise of gene-expression profiling technology. An in situ-synthesized 60-mer oligonucleotide microarray designed to detect transcripts from all mouse genes was validated, as well as a set of exogenous RNA controls derived from the yeast genome (made freely available without restriction), which allow quantitative estimation of absolute endogenous transcript abundance

Lunar regolith and structure mechanics

The topics are presented in viewgraph form and include the following: modeling of regolith-structure interaction in extraterrestrial constructed facilities; densification of lunar soil simulant; and vibration assisted penetration of lunar soil simulant

Off-shell effects in dilepton production from hot interacting mesons

The production of dielectrons in reactions involving a_1 mesons and pions is studied. We compare results obtained with different phenomenological Lagrangians that have been used in connection with hadronic matter and finite nuclei. We insist on the necessity for those interactions to satisfy known empirical properties of the strong interaction. Large off-shell effects in dielectron production are found and some consequences for the interpretation of heavy ion data are outlined. We also compare with results obtained using experimentally-extracted spectral functions.Comment: 14 pages, LaTeX2e, 2 figure

Chiral Lagrangian for strange hadronic matter

A generalized Lagrangian for the description of hadronic matter based on the linear $SU(3)_L \times SU(3)_R$ $\sigma$-model is proposed. Besides the baryon octet, the spin-0 and spin-1 nonets, a gluon condensate associated with broken scale invariance is incorporated. The observed values for the vacuum masses of the baryons and mesons are reproduced. In mean-field approximation, vector and scalar interactions yield a saturating nuclear equation of state. We discuss the difficulties and possibilities to construct a chiral invariant baryon-meson interaction that leads to a realistic equation of state. It is found that a coupling of the strange condensate to nucleons is needed to describe the hyperon potentials correctly. The effective baryon masses and the appearance of an abnormal phase of nearly massless nucleons at high densities are examined. A nonlinear realization of chiral symmetry is considered, to retain a Yukawa-type baryon-meson interaction and to establish a connection to the Walecka-model.Comment: Revtex, submitted to Phys. Rev.

Imaging-guided chest biopsies: techniques and clinical results

Background This article aims to comprehensively describe indications, contraindications, technical aspects, diagnostic accuracy and complications of percutaneous lung biopsy. Methods Imaging-guided biopsy currently represents one of the predominant methods for obtaining tissue specimens in patients with lung nodules; in many cases treatment protocols are based on histological information; thus, biopsy is frequently performed, when technically feasible, or in case other techniques (such as bronchoscopy with lavage) are inconclusive. Results Although a coaxial system is suitable in any case, two categories of needles can be used: fine-needle aspiration biopsy (FNAB) and core-needle biopsy (CNB), with the latter demonstrated to have a slightly higher overall sensitivity, specificity and accuracy. Conclusion Percutaneous lung biopsy is a safe procedure even though a few complications are possible: pneumothorax, pulmonary haemorrhage and haemoptysis are common complications, while air embolism and seeding are rare, but potentially fatal complications

Defining Developmental Potency and Cell Lineage Trajectories by Expression Profiling of Differentiating Mouse Embryonic Stem Cells

Biologists rely on morphology, function and specific markers to define the differentiation status of cells. Transcript profiling has expanded the repertoire of these markers by providing the snapshot of cellular status that reflects the activity of all genes. However, such data have been used only to assess relative similarities and differences of these cells. Here we show that principal component analysis of global gene expression profiles map cells in multidimensional transcript profile space and the positions of differentiating cells progress in a stepwise manner along trajectories starting from undifferentiated embryonic stem (ES) cells located in the apex. We present three ‘cell lineage trajectories’, which represent the differentiation of ES cells into the first three lineages in mammalian development: primitive endoderm, trophoblast and primitive ectoderm/neural ectoderm. The positions of the cells along these trajectories seem to reflect the developmental potency of cells and can be used as a scale for the potential of cells. Indeed, we show that embryonic germ cells and induced pluripotent cells are mapped near the origin of the trajectories, whereas mouse embryo fibroblast and fibroblast cell lines are mapped near the far end of the trajectories. We suggest that this method can be used as the non-operational semi-quantitative definition of cell differentiation status and developmental potency. Furthermore, the global expression profiles of cell lineages provide a framework for the future study of in vitro and in vivo cell differentiation