Spindle rotation in human cells is reliant on a MARK2-mediated equatorial spindle-centering mechanism

This work was supported by a Cancer Research UK Career Development Award (C28598/A9787), Biotechnology and Biological Sciences Research Council Project grant (BB/R01003X/1), and a Queen Mary University of London Laboratory startup grant to V.M. Draviam, a Universiti Brunei Darussalam PhD studentship to I. Zulkipli, a Queen Mary University of London PhD studentship to M. Hart, a London Interdisciplinary Biosciences Consortium Biotechnology and Biological Sciences Research Council–Doctoral Training Partnerships PhD studentship to D. Dang (cosupervised by V.M. Draviam and N. Sastry; BB/M009513/1), and an Islamic Development Bank PhD studentship to P. Gul

Osteoclasts are dispensable for hematopoietic stem cell maintenance and mobilization

The mobilization of hematopoietic stem cells does not require osteoclasts, which may even have an inhibitory effect

Prevention of hypoglycemia by intermittent-scanning continuous glucose monitoring device combined with structured education in patients with type 1 diabetes mellitus : A randomized, crossover trial

Aims: We conducted a randomized, crossover trial to compare intermittent-scanning continuous glucose monitoring (isCGM) device with structured education (Intervention) to self-monitoring of blood glucose (SMBG) (Control) in the reduction of time below range. Methods: This crossover trial involved 104 adults with type 1 diabetes mellitus (T1DM) using multiple daily injections. Participants were randomly allocated to either sequence Intervention/Control or sequence Control/Intervention. During the Intervention period which lasted 84 days, participants used the first-generation FreeStyle Libre (Abbott Diabetes Care, Alameda, CA, USA) and received structured education on how to prevent hypoglycemia based on the trend arrow and by frequent sensor scanning (≥10 times a day). Confirmatory SMBG was conducted before dosing insulin. The Control period lasted 84 days. The primary endpoint was the decrease in the time below range (TBR; <70 mg/dL). Results: The time below range was significantly reduced in the Intervention arm compared to the Control arm (2.42 ± 1.68 h/day [10.1 %±7.0 %] vs 3.10 ± 2.28 h/day [12.9 %±9.5 %], P = 0.012). The ratio of high-risk participants with low blood glucose index >5 was significantly reduced (8.6 % vs 23.7 %, P < 0.001). Conclusions: The use of isCGM combined with structured education significantly reduced the time below range in patients with T1DM

Hao1 Is Not a Pathogenic Factor for Ectopic Ossifications but Functions to Regulate the TCA Cycle In Vivo

Ossification of the posterior longitudinal ligament (OPLL), a disease characterized by the ectopic ossification of a spinal ligament, promotes neurological disorders associated with spinal canal stenosis. While blocking ectopic ossification is mandatory to prevent OPLL development and progression, the mechanisms underlying the condition remain unknown. Here we show that expression of hydroxyacid oxidase 1 (Hao1), a gene identified in a previous genome-wide association study (GWAS) as an OPLL-associated candidate gene, specifically and significantly decreased in fibroblasts during osteoblast differentiation. We then newly established Hao1-deficient mice by generating Hao1-flox mice and crossing them with CAG-Cre mice to yield global Hao1-knockout (CAG-Cre/Hao1flox/flox; Hao1 KO) animals. Hao1 KO mice were born normally and exhibited no obvious phenotypes, including growth retardation. Moreover, Hao1 KO mice did not exhibit ectopic ossification or calcification. However, urinary levels of some metabolites of the tricarboxylic acid (TCA) cycle were significantly lower in Hao1 KO compared to control mice based on comprehensive metabolomic analysis. Our data indicate that Hao1 loss does not promote ectopic ossification, but rather that Hao1 functions to regulate the TCA cycle in vivo

Automatización y control regulatorio de una columna de destilación extractiva a nivel planta piloto para la producción de etanol anhidro

La destilación extractiva es una de las principales tecnologías empleadas para la deshidratación de etanol y así usarlo como carburante. Entre los solventes que se pueden utilizar la glicerina presenta un alto potencial en cuanto al consumo energético de la operación. Por otra parte no existe una única metodología o algoritmo para seleccionar la estrategia de control de una columna de destilación puesto que cada sistema opera a diferentes condiciones y se presentan diferentes objetivos de control. Este trabajo aborda el estudio de dicho sistema a un nivel de simulación en estado estacionario para encontrar los parámetros de operación del proceso así como en estado dinámico para determinar la operabilidad y controlabilidad del sistema para finalmente seleccionar la estrategia de control a implementar en una columna real. Dicha estrategia de control se implemento en una columna de destilación a escala planta piloto para lo cual fue necesario realizar la instrumentación, automatización y configuración de 11 lazos de control en la columna. Finalmente, se ejecutan corridas experimentales para observar la dinámica del sistema, parámetros de operación reales y seleccionar una metodología de arranque y operación del sistema. / Abstract. Extractive distillation is one of the main technologies used for dehydration of ethanol and so use it as fuel. Among the solvents that can be used glycerol has a high potential in terms of energy consumption in the operation. Moreover there is no single methodology or algorithm to select the control strategy of a distillation column because each system operates at different conditions and have different control objectives. This paper deals with the study of this system to a level of steady-state simulation to find the process operating parameters as well as dynamic conditions to determine the operability and controllability of the system to finally select the control strategy to implement in a real column. This control strategy was implemented in a distillation column pilot scale for which it was necessary instrumentation, automation and configuration of 11 control loops in the column. Finally, experimental runs were performed to observe the dynamics of the system, actual operating parameters and select a startup and operation methodology.Maestrí