22 research outputs found
Impaired reproductive function and fertility preservation in a woman with a dyskeratosis congenita
A single-cell assay for telomere DNA content shows increasing telomere length heterogeneity, as well as increasing mean telomere length in human spermatozoa with advancing age
Presence of circulating folic acid in plasma and its relation with dietary intake, vitamin B complex concentrations and genetic variants
Ectopic callose deposition into woody biomass modulates the nano2 architecture of macrofibrils
Plant biomass plays an increasingly important role in the circular bioeconomy, replacing non-renewable fossil resources. Genetic engineering of this lignocellulosic biomass could benefit biorefinery transformation chains by lowering economic and technological barriers to industrial processing. However, previous efforts have mostly targeted the major constituents of woody biomass: cellulose, hemicellulose and lignin. Here we report the engineering of wood structure through the introduction of callose, a polysaccharide novel to most secondary cell walls. Our multiscale analysis of genetically engineered poplar trees shows that callose deposition modulates cell wall porosity, water and lignin contents and increases the lignin–cellulose distance, ultimately resulting in substantially decreased biomass recalcitrance. We provide a model of the wood cell wall nano-architecture engineered to accommodate the hydrated callose inclusions. Ectopic polymer introduction into biomass manifests in new physico-chemical properties and offers new avenues when considering lignocellulose engineering
Decreased expression of TERT and telomeric proteins as human ovaries age may cause telomere shortening
Cell-free DNA and telomere length among women undergoing in vitro fertilization treatment
Melatonin improves age-induced fertility decline and attenuates ovarian mitochondrial oxidative stress in mice
Fast modulation of visual perception by basal forebrain cholinergic neurons
The basal forebrain provides the primary source of cholinergic input to the cortex, and it has a crucial function in promoting wakefulness and arousal. However, whether rapid changes in basal forebrain neuron spiking in awake animals can dynamically influence sensory perception is unclear. Here we show that basal forebrain cholinergic neurons rapidly regulate cortical activity and visual perception in awake, behaving mice. Optogenetic activation of the cholinergic neurons or their V1 axon terminals improved performance of a visual discrimination task on a trial-by-trial basis. In V1, basal forebrain activation enhanced visual responses and desynchronized neuronal spiking; these changes could partly account for the behavioral improvement. Conversely, optogenetic basal forebrain inactivation decreased behavioral performance, synchronized cortical activity and impaired visual responses, indicating the importance of cholinergic activity in normal visual processing. These results underscore the causal role of basal forebrain cholinergic neurons in fast, bidirectional modulation of cortical processing and sensory perception.National Institute of Mental Health (U.S.) (Grant RC1-MH088434)National Institute of Neurological Disorders and Stroke (U.S.) (Ruth L. Kirschstein National Research Service Award F31NS059258