VIBRIOSIS PADA IKAN DAN ALTERN ATIFCARA PENANGGULANGANNYA

kata kunci : vibriosis dan cara penanggulangannya pada ika

Vaksinasi monovalen dan polivalen vaksin untuk mengatasi serangan Aeromonas hydrophila pada ikan lele (Clarias sp.). (Monovalent And Polyvalent Vaccination On Walking Catfish (Clarias sp.) Against Aeromonas hydrophila).

Aeromonas hidrophila is an opportunistic pathogen that has always caused high mortality in catfish farming. The use of medication is not significantly effective to control the disease. On the other hand medication can cause pollution and increase the number of resistant strains of bacteria to the drugs. The use of vaccination seems promising in controlling the disease. However the problem is that A. hydrophila has antigenic heterogenicity. The objectives of the study were to the eficacy of monovalent and polyvalent vaccines various methods of vaccination (oral, waterborne and i.m inâ¢jection), in various ages of cat fish fingerlings, in passive vaccination, and the pro⢠duction of antibody of vaccinated fish. tsi The result showed that the eficacy of polyvalent vaccine was higher than monovalent vaccine especially in i.m injection. In general i.m inject ion was more effective than oral and waterborne vaccinations. Passive vaccination could elevate the resistency of catfish fry until about 50 days after hatching. The mortality of fry from vaccinated breeder was significantly higher than the mortality of from unvaccinated breeder. By waterborne infection the mortality of fry from vaccinated breeder on 21, 35 and 50 days after hatching were 3.3%, 0% and 24.17%. While the fry froM unvaccinated breeder were 95.0%, 60.0% and 35,0%. The antibody of vaccinated fish was still detected until 12 weeks after vaccination

VACCINATION WITH VIBRIO ANGUILLARUM AND VIBRIO ORDALII BACTERINSAGAINST VIBRIOSIS

key words:Vaccination with vibrio Anguillarum - Vibrio ordalii bacterins against vibriosi

Karakteristik Aeromonas Hydrophila

In tisari Aeromonas hydrophila adalah bakteri penyebab penyakit ikan air tawar yang terpenting di Indonesia. Bakteri ini sangat ganas dan dapat menyebabkan kematian lebih dari 60% dalam waktu sekitar 7 hari. Penggunaan vaksin memberi harapan cukup baik, tetapi untuk pengembangannya masih mengalami hambatan, karena karakteristik bakteri ini belum banyak diketahui dan adanya heterogenisitas antigenik. Tujuan penelitian adalah untuk mengetahui sifat morfologi, biokimia, serologi dan patogenisitas beberapa isolat A. hydrophila dari Daerah lstimewa Yogyakarta clan Sawa Tengah bagian selatan. Hasil penelitian menunjukkan bahwa A. hydrophila ternyata dapat merupakan penyebab infeksi primer pada ikan lele. Bakteri ini bersifat Gram negatif, berbentuk batang, koloni bulat, cembung, berwarna kekuning-kuningan, dan mempunyai variasi biokimia. Dari 23 isolat yang memfermentasi glukosa dan membentuk gas 82,61%, laktosa 73,9%, sukrosa 100%, monitol 4,76%, dulcitol 8,69%, sorbitol 8,69%, arabinosa 60,69%, adonitol 13,04% dan raffinosa 26,09%. Sedang uji serologi menunjukkan bahwa diantara isolat ada yang bersifat reaksi silang dan mempunyai common antigen. Dosis letal (LD50) bakteri berkisar antara (5,78. x 104) â (3,35 x WI) seliml, dengan rata-rata waktu kematian 7,5 â 9,7 hart. Suntikan eksotoksin ternyata dapat menimbulkan kematian dan gejala yang sama dengan infeksi oleh sel utuh secara alami atau laboratoris. Abstract Aeromonas hydrophila is one of the most important bacterial pathogen in freshwater fishes in Indonesia. The bacteria can cause mortality more than 60% in cultured fish in approximately 7 days. Vaccination has a good prospect to salve the disease problem cause by MAS. However, there are some problems to develop mass production of A. hydrophila vaccine. Because the bacteria has antigenic heterogenicity and the characteristics are nor well understood

Isolation And Characterization Of Biomolecules Of Monodon baculovirus Isolates

Abstract: Monodon baculovirus (MBV) is one of the most important viral disease in shrimp culture because it caused high mortality rate of all shrimp life stages in wide culture regions. The objectives of this study were to purify and characterize biomolecules of OB and MBV virion taken from shrimp hepatopancreas of Tiger shrimp (Penaeus monodon). Shrimp samples were taken from brackish water ponds in West Java, Central Java, and East Java provinces. Number of molecules and molecular weight of viral protein and OB, DNA from and the shape of MBV virion were determined using purified OB and extracted DNA from hepatopancreas of the shrimp. The result indicated that shrimp samples from various sample areas was only infected by one type of virus, that is Monodon baculovirus. The MBV genom is DNA with size more than 10 kbp, the shape of MBV virion is rod and composed by at least nine dominant protein units. Key words:biomolecules, Monodon baculovirus, Penaeus monodo

Perubahan Kadar Hormon Testosteron dan Progesteron, Korelasinya dengan Indeks Gonado Somatik dan Tingkat Kematangan Gonad pada Ikan Brek (Puntius orphoides Cuvier & Valenciennes, 1842)

Penelitian ini bertujuan mengetahui perubahan kadar hormon testosteron pada ikan jantan dan hormon progesteron pada ikan betina, serta korelasinya dengan Indeks Gonado Somatik (IGS) dan Tingkat Kematangan Gonad (TKG). Sampel ikan diperoleh setiap bulan, selama Juni 2009–Mei 2010, menggunakan teknik simple random sampling. Pengukuran hormon dilakukan dengan metode ELISA. Analisis data dilakukan terhadap 120 ekor ikan jantan dan 120 ekor ikan betina, meliputi uji ’F’ terhadap perubahan kadar testosteron dan progesteron, IGS dan TKG setiap bulan selama satu siklus reproduksi. Selain itu, dilakukan analisis korelasi antara kadar testosteron dan progesteron dengan IGS dan TKG. Hasil penelitian menunjukkan bahwa kadar testosteron dalam darah ikan brek jantan dan kadar progesteron dalam darah ikan brek betina selama satu tahun penelitian mengalami perubahan. Kadar testosteron memiliki kisaran antara 0,10−0,35 ng/mL, sedangkan kisaran progesteron antara 0,250,60 ng/mL, Puncak tertinggi kadar testosteron sebesar 0,203 n g/ mL dan 0,224 n g/ mL terjadi pada bulan SeptemberOktober, demikian pula puncak tertinggi kadar progesteron sebesar 0,645 g/mL dan 0,091 n g/mL. Korelasi kadar testosteron dengan IGS adalah positif nyata, demikian pula kadar progesteron dengan IGS. Korelasi kadar testosteron dengan TKG positif, demikian pula kadar progesteron dengan TKG

EFIKASI VAKSIN DAN KEMANGKUSAN TETRAS1KLIN UNTUK PENANGGULANGAN PENYAKIT MAS : PADA PENDEDERAN LELE DUMBO (Clarias gariepinus)THE EFFICACY OF VACCINE AND THERAPEUTIC EFFECT INFECTION OF TETRACYCLINE AGAINST MAS : IN NURS

The study was conducted to know the efficacy of vaccine and therapeutic effect of tetracycline against Aeromonas hydrophila in African catfish (Clarias gariepinus) during nursing period. The locations of study were Cangkringan, Miati, experimental pond of the Department of Fisheries University of Gadjah Mada and Bantul regency. The fish were vaccinated or medicated by immersion method and released into the ponds soon after vaccination or medication. The results indicated that survival rates of vaccinated fish (47.03%) and medicated fish (38.86%) were significantly different (

ia PADA LELE DUMBO (Clarias gariepinus) TEST OF ANTIGENICITY AND EFFICACY OF Aeromonas hydrophilia VACCINES IN AFRICAN CATFISH (Clarias gariepinus)

ABSTRACT: Study was conducted to select the best antigens (Ags) of Aeromonas hydrophila for, making monovalent and polyvalent vaccines. Few mice were injected intraperitonealy by 0.1 ml each of G, H and supernatant Ags_ The best Ag of every isolate was selected by rapid slide agglutination method. Monovalent and polyvalent vaccines were made, from selected Ags. The efficacy of vaccines was measured by immersion and oral vaccination methods in African catfish fry (Clarias gariepinus). Challenge was conducted by immersion method for 30 minutes. The results indicated that Ab titers of mice were selected since the first week after Ag injection and reached the peak\u27 between the second and the third week. Three isolates (PA 01, PA 05 and BA 02) had maximum Ab titer (8-10) from H Ag. Two other isolates (PA 06 and PA 07) from 0 Ag (8). The supernatant of all isolates reached maximum Ab titer between 6-8. The efficacy test showed that the mortality rates of vaccinated fry were significantly lower than that in unvaccinated fry (

Prevalensi dan derajat infeksi myxobolus sp. Pada insang benih karper (cyprinus carpio) di kabupaten sleman

Abstract This study was conducted to know the prevalence and degree of infection of Myxobolus sp. in common carp (Cyprinus carpio) fry in Sleman regency (Cangkringan, Kalasan, Ngemplak, and Turi sub districts). The selection of sub districts used purposive sampling based on the difference of water quality. Fish sampling was conducted in June and December 2001. Data were analysed by using analysis of variance (ANOVA) and regression analysis.The results showed that the prevalence of Myxobolus sp. in Cangkringan, Kalasan, Ngemplak, and Turi sub districts were 10%, 36.67%, 1.67%, and 26.67%, respectively. Regression analysis indicated that pH influenced the prevalence of Myxobolus sp. in Sleman regency.The degree of infection of Myxobolus sp. in Cangkringan, Kalasan, Ngemplak, and Turi sub districts were 326.250.51.5 parasites/fish, respectively. Regression analysis indicated that free CO2, pH, and organik matters have positive correlation to the degree of infection of Myxobolus sp. in gill of common carp fry in Sleman regency. Key words: common carp fry, degree of infection, Myxobolus sp., prevalenc