Abstract:
Monodon baculovirus (MBV) is one of the most important viral disease in shrimp culture because it caused high mortality rate of all shrimp life stages in wide culture regions.
The objectives of this study were to purify and characterize biomolecules of OB and MBV virion taken from shrimp hepatopancreas of Tiger shrimp (Penaeus monodon). Shrimp samples were taken from brackish water ponds in West Java, Central Java, and East Java provinces. Number of molecules and molecular weight of viral protein and OB, DNA from and the shape of MBV virion were determined using purified OB and extracted DNA from hepatopancreas of the shrimp.
The result indicated that shrimp samples from various sample areas was only infected by one type of virus, that is Monodon baculovirus. The MBV genom is DNA with size more than 10 kbp, the shape of MBV virion is rod and composed by at least nine dominant protein units.
Key words:biomolecules, Monodon baculovirus, Penaeus monodo
