A membrane network of receptors and enzymes for adenine nucleotides and nucleosides

AbstractMost cells express more than one receptor plus degrading enzymes for adenine nucleotides or nucleosides, and cellular responses to purines are rarely compatible with the actions of single receptors. Therefore, these receptors are viewed as components of a combinatorial receptor web rather than self-dependent entities, but it remained unclear to what extent they can associate with each other to form signalling units. P2Y1, P2Y2, P2Y12, P2Y13, P2X2, A1, A2A receptors and NTPDase1 and -2 were expressed as fluorescent fusion proteins which were targeted to membranes and signalled like the unlabelled counterparts. When tested by FRET microscopy, all the G protein-coupled receptors proved able to form heterooligomers with each other, and P2Y1, P2Y12, P2Y13, A1, A2A, and P2X2 receptors also formed homooligomers. P2Y receptors did not associate with P2X, but G protein-coupled receptors formed heterooligomers with NTPDase1, but not NTPDase2. The specificity of prototypic interactions (P2Y1/P2Y1, A2A/P2Y1, A2A/P2Y12) was corroborated by FRET competition or co-immunoprecipitation. These results demonstrate that G protein-coupled purine receptors associate with each other and with NTPDase1 in a highly promiscuous manner. Thus, purinergic signalling is not only determined by the expression of receptors and enzymes but also by their direct interaction within a previously unrecognized multifarious membrane network

Evidence for Different Freeze-Out Radii of High- and Low-Energy Pions Emitted in Au+Au Collisions at 1 GeV/nucleon

Double differential production cross sections of negative and positive pions and the number of participating protons have been measured in central Au+Au collisions at 1 GeV per nucleon incident energy. At low pion energies the pi^- yield is strongly enhanced over the pi^+ yield. The energy dependence of the pi^-/pi^+ ratio is assigned to the Coulomb interaction of the charged pions with the protons in the reaction zone. The deduced Coulomb potential increases with increasing pion c.m. energy. This behavior indicates different freeze-out radii for different pion energies in the c.m.~frame.Comment: IKDA is the Institute for Nuclear Physics in Darmstadt/German

Enhanced Out-of-plane Emission of K+ Mesons observed in Au+Au Collisions at 1 AGeV

The azimuthal angular distribution of K+ mesons has been measured in Au + Au collisions at 1 AGeV. In peripheral and semi-central collisions, K+ mesons preferentially are emitted perpendicular to the reaction plane. The strength of the azimuthal anisotropy of K+ emission is comparable to the one of pions. No in-plane flow was found for K+ mesons near projectile and target rapidity.Comment: Accepted for publication in Phys. Rev.Let

Photoproduction at collider energies: from RHIC and HERA to the LHC

We present the mini-proceedings of the workshop on ``Photoproduction at collider energies: from RHIC and HERA to the LHC'' held at the European Centre for Theoretical Studies in Nuclear Physics and Related Areas (ECT*, Trento) from January 15 to 19, 2007. The workshop gathered both theorists and experimentalists to discuss the current status of investigations of high-energy photon-induced processes at different colliders (HERA, RHIC, and Tevatron) as well as preparations for extension of these studies at the LHC. The main physics topics covered were: (i) small-$x$ QCD in photoproduction studies with protons and in electromagnetic (aka. ultraperipheral) nucleus-nucleus collisions, (ii) hard diffraction physics at hadron colliders, and (iii) photon-photon collisions at very high energies: electroweak and beyond the Standard Model processes. These mini-proceedings consist of an introduction and short summaries of the talks presented at the meeting

ISSUES IN HIGH-RESOLUTION ATMOSPHERIC MODELING IN COMPLEX TOPOGRAPHY --THE HiRCoT WORKSHOP

During the past years the atmospheric modeling community, both from the application and pure research perspectives, has been facing the challenge of high resolution numerical modeling in places with complex topography. In February 2012, as a result of the collaborative efforts of the Institute of Meteorology of the University of Natural Resources and Life Sciences (BOKUMet), the Arctic Region Supercomputing Center (ARSC), the Institute of Meteorology and Geophysics of the University of Innsbruck (IMG) and the enthusiasm of the scientific community, the HiRCoT workshop was held in Vienna, Austria. HiRCoT objectives were to: 1) Identify the problems encountered with numerical modeling at grid spacing lower than 1 km over complex terrain, that is, understand the key areas that are troublesome and formulate the key questions about them; 2) Map out possibilities on how to address these issues; 3) Allow the researchers to discuss the issues on a shared platform (online through a wikipage and face-to-face). This manuscript presents an overview of the topics and research priorities discussed in the workshop

Transition Radiation Spectroscopy with Prototypes of the ALICE TRD

We present measurements of the transition radiation (TR) spectrum produced in an irregular radiator at different electron momenta. The data are compared to simulations of TR from a regular radiator.Comment: 4 pages, 5 Figures, Proceedings for "TRDs for the 3rd millennium" (Sept. 4-7, 2003, Bari, Italy

Heavy-flavour and quarkonium production in the LHC era: from proton-proton to heavy-ion collisions

This report reviews the study of open heavy-flavour and quarkonium production in high-energy hadronic collisions, as tools to investigate fundamental aspects of Quantum Chromodynamics, from the proton and nucleus structure at high energy to deconfinement and the properties of the Quark-Gluon Plasma. Emphasis is given to the lessons learnt from LHC Run 1 results, which are reviewed in a global picture with the results from SPS and RHIC at lower energies, as well as to the questions to be addressed in the future. The report covers heavy flavour and quarkonium production in proton-proton, proton-nucleus and nucleus-nucleus collisions. This includes discussion of the effects of hot and cold strongly interacting matter, quarkonium photo-production in nucleus-nucleus collisions and perspectives on the study of heavy flavour and quarkonium with upgrades of existing experiments and new experiments. The report results from the activity of the SaporeGravis network of the I3 Hadron Physics programme of the European Union 7th Framework Programme

Position Reconstruction in Drift Chambers operated with Xe, CO2 (15%)

We present measurements of position and angular resolution of drift chambers operated with a Xe,CO$_2$(15%) mixture. The results are compared to Monte Carlo simulations and important systematic effects, in particular the dispersive nature of the absorption of transition radiation and non-linearities, are discussed. The measurements were carried out with prototype drift chambers of the ALICE Transition Radiation Detector, but our findings can be generalized to other drift chambers with similar geometry, where the electron drift is perpendicular to the wire planes.Comment: 30 pages, 18 figure