The influence of brood chickens by-products processing with probiotic culture starter on change of their functional and technological parameters.

By-products are the potential source of animal protein obtained from brood chickens and egg-laying hens. Certain by-products like gizzards and combs are quite tough and possess low nutritional and biological value due to their high content of connective tissue. Biotechnological processing improves the quality parameters of collagen-containing by-products. In this article a probiotic starter culture of propionic acid bacteria, which have high proteolytic activity, was used to treat the gizzards and combs of brood chickens. Before processing of by-products with starter culture, physical and chemical parameters and the yield of by-products in relation to poultry live weight were analyzed and recorded. 5%, 10% and 15% starter culture were added to the tested samples of chopped by-products, the samples were kept at a temperature of 30 °C, and every 4 hours the following functional and technological parameters were monitored: moisture binding capacity, water holding capacity (MBC and WHC) and yield of the product after heat treatment. The results proved that increase of starter culture amount and longer exposure of by-products to hydrolysis led to decrease of functional and technological parameters values, but for the combs those parameters remained at a sufficiently high level compared to the gizzards, as the gizzards were exposed to more intense hydrolysis than combs. The decrease in the pH value correlated with the dynamics of MBC and WHC changes; and dynamics of the product yield after the heat treatment. Also the stained histological preparations were studied in order to assess the influence of biotechnological processing on by-products microstructure, where significant differences were found in the morphological structure of muscle and collagen fibers of hydrolysates of combs and gizzards exposed to action of bacterial concentrate. The results of rheological studies showed that hydrolyzed chicken combs differed from gizzards; the combs were denser and featured more elastic structure due to a lower degree of hydrolysis by bacterial enzymes. In general, the properties of collagen-containing by-products (muscular gizzards and combs) change significantly after being exposed to enzymes of propionic acid bacteria

Structural and magnetic dimers in the spin-gapped system CuTe2O5

We investigated the magnetic properties of the system CuTe2O5 by susceptibility and electron spin resonance measurements. The anisotropy of the effective g-factors and the ESR linewidth indicates that the anticipated structural dimer does not correspond to the singlet-forming magnetic dimer. Moreover, the spin susceptibility of CuTe2O5 can only be described by taking into account interdimer interactions of the same order of magnitude than the intradimer coupling. Analyzing the exchange couplings in the system we identify the strongest magnetic coupling between two Cu ions to be mediated by super-super exchange interaction via a bridging Te ligand, while the superexchange coupling between the Cu ions of the structural dimer only results in the second strongest coupling

The Role of ATP-Sensitive Potassium Channels and Nitric Oxide in the Protective Effect of Preconditioning of the Brain

© 2017 Springer Science+Business Media, LLC, part of Springer Nature Objective. The role of ATP-dependent potassium (K + ATP ) channels in the neuroprotective effect of ischemic (IPre) and pharmacological (PPre) preconditioning and changes in blood levels of nitric oxide (NO) metabolites were studied in conditions of cerebral ischemia. Materials and methods. Ischemic stroke (IS) was modeled in male rats (n = 86) by electrocoagulation of a branch of the middle cerebral artery (MCA). The nonselective K + ATP channel blocker glibenclamide and the K + ATP channel activator diazoxide were used. IPre and PPre were performed one day before MCA occlusion. Blood concentrations of NO, nitrates (NO 3 – ) and nitrites (NO 2 – ) were determined in experimental animals at 5, 24, and 72 h after MCA occlusion. Results. IPre decreased the lesion zone by 37% (p < 0.05), while prior administration of glibenclamide countered the action of IPre. The protective effect of PPre was analogous to that of IPre. Decreases in blood levels of oxygenated R-conformers of hemoglobin-bound NO (Hb-NO) were seen 5 h after MCA occlusion, with an inversely proportional increase in the concentration of nonoxygenated T-conformers; there were also increases in NO 3 – and NO 2 – concentrations. NO 3 – and NO 2 – levels showed normalization by one day after MCA occlusion, along with changes in the concentrations of Hb-NO complexes – R-conformers dominated, while the blood level of T-conformers reached a minimum. Furthermore, by 24 h there was a correlation between blockade of K + ATP channels and decreases in serum NO 3 – and NO 2 – levels (p < 0.03). Conclusions. The neuroprotective effect of preconditioning was due to activation of K + ATP channels. Analysis of blood levels of NO metabolites in rats with IS showed that Hb-NO complexes in the R-conformation stored and carried NO to the tissues, releasing NO on occurrence of the R → T transition in ischemic conditions

The Role of the Danish Church in the Fight against the COVID-19 Pandemic and the Significance of This Experience for the Russian Federation

The social responsibility of religious organizations in modern conditions is reflected in a variety of activities carried out by religious institutions and differ depending on both the confessional affiliation of these institutions and the specific structural territorial unit where they take place. The presented work is devoted to a detailed analysis of the peculiarities of the manifestation of one of the directions of social responsibility of the Evangelical Lutheran Church of Denmark (ELCD), associated with minimizing the consequences of COVID-19 on the example of its individual dioceses. The dioceses characterized by the highest proportion of adherents in the population structure (Diocese of Viborg, Ribe, Aalborg and Lolland-Faster) were selected to clearly reflect the trends taking place. The subject of the study is the social activities of the ELCD to mitigate the consequences of COVID-19 for parishioners, the features of the implementation of which are considered on the example of structural units at the regional level. The article substantiates the increased importance of the ELCD in the development of the region, since the designated institution largely assumes the social obligations of the state (duplicates several functions) in connection with the risks of the spread of coronavirus infection. To achieve the goal, methods of analysis of factual and economic-statistical information, comparative comparison were used. The experience of a religious organization in dealing with the consequences of the COVID-19 pandemic is of interest to the public, government bodies and authorities, as well as religious organizations in other countries. The results of the study can be useful in developing a strategy for the development of state-religious relations in Russia, where there are trends of strengthening the social and economic role of religious organizations

Social Problems of Modern Society: Alcoholism In Russia

The article analyzes alcoholism as a social problem of modern Russian society. The situation of alcohol consumption in the Russian Federation and the factors that have a negative impact on the increase in the level of alcohol consumption in the country.В статье проводится анализ алкоголизма как социальной проблемы современного российского общества. Рассматривается ситуация потребления алкогольной продукции в Российской Федерации и факторы, оказывающие отрицательное влияние на повышение уровня потребления алкоголя в стране

Cold-induced changes in gene expression in brown adipose tissue, white adipose tissue and liver

Cold exposure imposes a metabolic challenge to mammals that is met by a coordinated response in different tissues to prevent hypothermia. This study reports a transcriptomic analysis in brown adipose tissue (BAT), white adipose (WAT) and liver of mice in response to 24 h cold exposure at 8°C. Expression of 1895 genes were significantly (P<0.05) up- or down-regulated more than two fold by cold exposure in all tissues but only 5 of these genes were shared by all three tissues, and only 19, 14 and 134 genes were common between WAT and BAT, WAT and liver, and BAT and liver, respectively. We confirmed using qRT-PCR, the increased expression of a number of characteristic BAT genes during cold exposure. In both BAT and the liver, the most common direction of change in gene expression was suppression (496 genes in BAT and 590 genes in liver). Gene ontology analysis revealed for the first time significant (P<0.05) down regulation in response to cold, of genes involved in oxidoreductase activity, lipid metabolic processes and protease inhibitor activity, in both BAT and liver, but not WAT. The results reveal an unexpected importance of down regulation of cytochrome P450 gene expression and apolipoprotein, in both BAT and liver, but not WAT, in response to cold exposure. Pathway analysis suggests a model in which down regulation of the nuclear transcription factors HNF4α and PPARα in both BAT and liver may orchestrate the down regulation of genes involved in lipoprotein and steroid metabolism as well as Phase I enzymes belonging to the cytochrome P450 group in response to cold stress in mice. We propose that the response to cold stress involves decreased gene expression in a range of cellular processes in order to maximise pathways involved in heat production

Rates of lobar atrophy in asymptomatic MAPT mutation carriers.

IntroductionThe aim of this study was to investigate the rates of lobar atrophy in the asymptomatic microtubule-associated protein tau (MAPT) mutation carriers.MethodsMAPT mutation carriers (n&nbsp;=&nbsp;14; 10 asymptomatic, 4 converters from asymptomatic to symptomatic) and noncarriers (n&nbsp;=&nbsp;13) underwent structural magnetic resonance imaging and were followed annually with a median of 9.2&nbsp;years. Longitudinal changes in lobar atrophy were analyzed using the tensor-based morphometry with symmetric normalization algorithm.ResultsThe rate of temporal lobe atrophy in asymptomatic MAPT mutation carriers was faster than that in noncarriers. Although the greatest rate of atrophy was observed in the temporal lobe in converters, they also had increased atrophy rates in the frontal and parietal lobes compared to noncarriers.DiscussionAccelerated decline in temporal lobe volume occurs in asymptomatic MAPT mutation carriers followed by the frontal and parietal lobe in those who have become symptomatic. The findings have implications for monitoring the progression of neurodegeneration during clinical trials in asymptomatic MAPT mutation carriers

Molecular bases of brain preconditioning

© 2017 Deryagin, Gavrilova, Gainutdinov, Golubeva, Andrianov, Yafarova, Buravkov and Koshelev. Preconditioning of the brain induces tolerance to the damaging effects of ischemia and prevents cell death in ischemic penumbra. The development of this phenomenon is mediated by mitochondrial adenosine triphosphate-sensitive potassium (KATP+) channels and nitric oxide signaling (NO). The aim of this study was to investigate the dynamics of molecular changes in mitochondria after ischemic preconditioning (IP) and the effect of pharmacological preconditioning (PhP) with the KATP+-channels opener diazoxide on NO levels after ischemic stroke in rats. Immunofluorescence-histochemistry and laser-confocal microscopy were applied to evaluate the cortical expression of electron transport chain enzymes, mitochondrial KATP+-channels, neuronal and inducible NO-synthases, as well as the dynamics of nitrosylation and nitration of proteins in rats during the early and delayed phases of IP. NO cerebral content was studied with electron paramagnetic resonance (EPR) spectroscopy using spin trapping. We found that 24 h after IP in rats, there is a two-fold decrease in expression of mitochondrial KATP+-channels (p = 0.012) in nervous tissue, a comparable increase in expression of cytochrome c oxidase (p = 0.008), and a decrease in intensity of protein S-nitrosylation and nitration (p = 0.0004 and p = 0.001, respectively). PhP led to a 56% reduction of free NO concentration 72 h after ischemic stroke simulation (p = 0.002). We attribute this result to the restructuring of tissue energy metabolism, namely the provision of increased catalytic sites to mitochondria and the increased elimination of NO, which prevents a decrease in cell sensitivity to oxygen during subsequent periods of severe ischemia

Exploration of structural, thermal, vibrational and spectroscopic properties of new noncentrosymmetric double borate Rb3NdB6O12

New noncentrosymmetric rare earth borate Rb3NdB6O12 is found in the ternary system Rb2O–Nd2O3–B2O3. The Rb3NdB6O12 powder was fabricated by solid state synthesis at 1050 K for 72 h and the crystal structure was obtained by the Rietveld method. Rb3NdB6O12 crystallized in space group R32 with unit cell parameters a = 13.5236(4), c = 31.162(1) Å, Z = 3. From DSC measurements, the reversible phase transition (I type) in Rb3NdB6O12 is observed at 852–936 K. The 200 μm thick tablet is transparent over the spectral range of 0.3–6.5 μm and the band gap is found as Eg ∼ 6.29 eV. Nonlinear optical response of Rb3NdB6O12 tested via SHG is estimated to be higher than that of K3YB6O12. Blue shift of Nd luminescent lines is found in comparison with other borates. The vibrational parameters of Rb3NdB6O12 are evaluated by experimental methods