On the Calibration of Full-polarization 86GHz Global VLBI Observations

We report the development of a semi-automatic pipeline for the calibration of 86 GHz full-polarization observations performed with the Global Millimeter-VLBI array (GMVA) and describe the calibration strategy followed in the data reduction. Our calibration pipeline involves non-standard procedures, since VLBI polarimetry at frequencies above 43 GHz is not yet well established. We also present, for the first time, a full-polarization global-VLBI image at 86 GHz (source 3C 345), as an example of the final product of our calibration pipeline, and discuss the effect of instrumental limitations on the fidelity of the polarization images. Our calibration strategy is not exclusive for the GMVA, and could be applied on other VLBI arrays at millimeter wavelengths. The use of this pipeline will allow GMVA observers to get fully-calibrated datasets shortly after the data correlation.Comment: 10 pages, 10 figures. Accepted for publication in A&

Search for the active ingredients from a 2-aminothiazole DMSO stock solution with antimalarial activity

Chemical decomposition of DMSO stock solutions is a common incident that can mislead biological screening campaigns. Here, we share our case study of 2-aminothiazole 1, originating from an antimalarial class that undergoes chemical decomposition in DMSO at room temperature. As previously measured biological activities observed against Plasmodium falciparum NF54 and for the target enzyme Pf IspE were not reproducible for a fresh batch, we tackled the challenge to understand where the activity originated from. Solvent- and temperature-dependent studies using HRMS and NMR spectroscopy to monitor the decomposition led to the isolation and in vitro evaluation of several fractions against Pf IspE. After four days of decomposition, we successfully isolated the oxygenated and dimerised compounds using SFC purification and correlated the observed activities to them. Due to the unstable nature of the two isolates, it is likely that they undergo further decomposition contributing to the overall instability of the compound

Scattering in the vicinity of relativistic jets: a method for constraining jet parameters

Relativistic jets of radio loud active galactic nuclei (AGN) produce highly directed, intense beams of radiation. A fraction of this beamed radiation scatters on the thermal plasma generally surrounding an AGN. The morphology of the scattered emission can thus provide constraints on the physical properties of the jet. We present a model to study the feasibility of constraining the parameters of a jet, especially its inclination angle and bulk Lorentz factor in this way. We apply our model to the well studied jet of M87 and the surrounding diffuse gas and find that the observational limits of the surface brightness measured in the region of the putative counterjet provide the tightest constraints on the jet parameters consistent with constraints derived by other methods. We briefly discuss the applicability of our model to other sources exhibiting relativistic motionsComment: 17 pages, 15 figures, to appear in A&A, 420, 33 (2004

Properties of Interfaces in the two and three dimensional Ising Model

To investigate order-order interfaces, we perform multimagnetical Monte Carlo simulations of the $2D$ and $3D$ Ising model. Following Binder we extract the interfacial free energy from the infinite volume limit of the magnetic probability density. Stringent tests of the numerical methods are performed by reproducing with high precision exact $2D$ results. In the physically more interesting $3D$ case we estimate the amplitude $F^s_0$ of the critical interfacial tension $F^s = F^s_0 t^\mu$ to be $F^s_0 = 1.52 \pm 0.05$. This result is in good agreement with a previous MC calculation by Mon, as well as with experimental results for related amplitude ratios. In addition, we study in some details the shape of the magnetic probability density for temperatures below the Curie point.Comment: 25 pages; sorry no figures include

The effect of systematics on polarized spectral indices

We study four particularly bright polarized compact objects (Tau A, Virgo A, 3C273 and Fornax A) in the 7-year WMAP sky maps, with the goal of understanding potential systematics involved in estimation of foreground spectral indices. We estimate the spectral index, the polarization angle, the polarization fraction and apparent size and shape of these objects when smoothed to a nominal resolution of 1 degree FWHM. Second, we compute the spectral index as a function of polarization orientation, alpha. Because these objects are approximately point sources with constant polarization angle, this function should be constant in the absence of systematics. However, computing it for the K- and Ka-band WMAP data we find strong index variations for all four sources. For Tau A, we find a spectral index beta=-2.59+-0.03 for alpha=30 degrees, and beta=-2.03+-0.01 for alpha=50 degrees. On the other hand, the spectral index between Ka and Q band is found to be stable. A simple elliptical Gaussian toy model with parameters matching those observed in Tau A reproduces the observed signal, and shows that the spectral index is in particular sensitive to the detector polarization angle. Based on these findings, we first conclude that estimation of spectral indices with the WMAP K-band polarization data at 1 degree scales is not robust. Second, we note that these issues may be of concern for ground-based and sub-orbital experiments that use the WMAP polarization measurements of Tau A for calibration of gain and polarization angles.Comment: 5 pages, 6 figures, submitted to ApJ; new figure and expanded conclusio

Akzeptanz, Rollendifferenzierung und geschlechtsspezifisches Verhalten in Dyaden bei Botanischen Bestimmungsübungen: Ein Vergleich zweier Medien

Die vorliegende Studie beschreibt geschlechts- und medienspezifische Effekte bei der Bestimmung von Pflanzen. Das Ziel bestand darin, explorativ zu untersuchen, welche Auswirkungen die Arbeit mit einer Online-Lernumgebung im Gegensatz zu bisherigen Bestimmungsbüchern auf die Prozesse in gemischt-geschlechtlichen Dyaden hat. Hierzu wurden vorrangig die Anzahlen medien- und pflanzenbezogener Aktionen der Probanden untersucht. Generell zeigt die Studie, dass keine schwerwiegenden geschlechtsspezifischen Effekte zu erwarten sind. Allerdings muss bei der Implementierung der Lernumgebung darauf geachtet werden, dass keine Rollendifferenzierung derart entsteht, dass innerhalb der Lerndyaden ein Proband den Computer bedient und der andere die Pflanze untersucht. Die Einstellung der Probanden gegenüber der neuen Lernumgebung war positiv, so dass von Seiten der Studenten keine Akzeptanzprobleme bezüglich der Implementierung in die Kurse zu erwarten sind

Targeting the IspD enzyme in the MEP pathway: identification of a novel fragment class

Enzymes of the 2-C-methylerythritol-d-erythritol 4-phosphate 2C-methyl-D-erythritol 4-phosphate (MEP) pathway (MEP pathway or non-mevalonate pathway) are responsible for the synthesis of universal precursors of the huge large and structurally diverse family of isoprenoids. This pathway is absent in humans, but present in many pathogenic organisms and plants, making it an attractive source of drug targets. Here, we present a high-throughput screening approach that led to the discovery of a novel fragment hit active against the third enzyme of the MEP pathway, PfIspD. A systematic SAR investigation afforded a novel chemical structure with a balanced activity-stability profile (16). Using a homology model of PfIspD, we proposed a putative binding mode for our newly identified inhibitors that sets the stage for structure-guided optimization

Targeting the IspD Enzyme in the MEP Pathway: Identification of a Novel Fragment Class

The enzymes of the 2-C-methylerythritol-d-erythritol 4-phosphate (MEP) pathway (MEP pathway or non-mevalonate pathway) are responsible for the synthesis of universal precursors of the large and structurally diverse family of isoprenoids. This pathway is absent in humans, but present in many pathogenic organisms and plants, making it an attractive source of drug targets. Here, we present a high-throughput screening approach that led to the discovery of a novel fragment hit active against the third enzyme of the MEP pathway, PfIspD. A systematic SAR investigation afforded a novel chemical structure with a balanced activity–stability profile (16). Using a homology model of PfIspD, we proposed a putative binding mode for our newly identified inhibitors that sets the stage for structure-guided optimization

Search for the Active Ingredients from a 2-Aminothiazole DMSO Stock Solution with Antimalarial Activity

Chemical decomposition of DMSO stock solutions is a common incident that can mislead biological screening campaigns. Here, we share our case study of 2-aminothiazole 1, originating from an antimalarial class that undergoes chemical decomposition in DMSO at room temperature. As previously measured biological activities observed against Plasmodium falciparum NF54 and for the target enzyme PfIspE were not reproducible for a fresh batch, we tackled the challenge to understand where the activity originated from. Solvent- and temperature-dependent studies using HRMS and NMR spectroscopy to monitor the decomposition led to the isolation and in vitro evaluation of several fractions against PfIspE. After four days of decomposition, we successfully isolated the oxygenated and dimerised compounds using SFC purification and correlated the observed activities to them. Due to the unstable nature of the two isolates, it is likely that they undergo further decomposition contributing to the overall instability of the compound

Zooming towards the Event Horizon - mm-VLBI today and tomorrow

Global VLBI imaging at millimeter and sub-millimeter wavelength overcomes the opacity barrier of synchrotron self-absorption in AGN and opens the direct view into sub-pc scale regions not accessible before. Since AGN variability is more pronounced at short millimeter wavelength, mm-VLBI can reveal structural changes in very early stages after outbursts. When combined with observations at longer wavelength, global 3mm and 1mm VLBI adds very detailed information. This helps to determine fundamental physical properties at the jet base, and in the vicinity of super-massive black holes at the center of AGN. Here we present new results from multi-frequency mm-VLBI imaging of OJ287 during a major outburst. We also report on a successful 1.3mm VLBI experiment with the APEX telescope in Chile. This observation sets a new record in angular resolution. It also opens the path towards future mm-VLBI with ALMA, which aims at the mapping of the black hole event horizon in nearby galaxies, and the study of the roots of jets in AGN.Comment: 6 pages, to appear in 11th European VLBI Network Symposium, ed. P. Charlot et al., Bordeaux (France), October 9-12, 201