324 research outputs found
SOCIOLINGUISTICS AND LINGUISTIC GEOGRAPHY
Sociolinguistics and linguistic geography should be considered as complementary rather than mutually contradictory approaches to the phenomena of language variation. Linguistic geography is a branch of historical linguistics based on samples of the stable and traditional, and necessarily somewhat biased in the selection of small communities, older informants, and traditional cultures; however, it provides a framework for interpreting studies of varied populations-in both rural and urban communities. The authors review criticisms of both linguistic geography and of sociolinguistics applications of linguistic geography, and suggest directions in which the findings of linguistic geography may be useful to sociolinguists and others in matters of interdisciplinary cooperation.http://web.ku.edu/~starjrn
Data Exploration, Quality Control and Testing in Single-Cell qPCR-Based Gene Expression Experiments
Cell populations are never truly homogeneous; individual cells exist in
biochemical states that define functional differences between them. New
technology based on microfluidic arrays combined with multiplexed quantitative
polymerase chain reactions (qPCR) now enables high-throughput single-cell gene
expression measurement, allowing assessment of cellular heterogeneity. However
very little analytic tools have been developed specifically for the statistical
and analytical challenges of single-cell qPCR data. We present a statistical
framework for the exploration, quality control, and analysis of single-cell
gene expression data from microfluidic arrays. We assess accuracy and
within-sample heterogeneity of single-cell expression and develop quality
control criteria to filter unreliable cell measurements. We propose a
statistical model accounting for the fact that genes at the single-cell level
can be on (and for which a continuous expression measure is recorded) or
dichotomously off (and the recorded expression is zero). Based on this model,
we derive a combined likelihood-ratio test for differential expression that
incorporates both the discrete and continuous components. Using an experiment
that examines treatment-specific changes in expression, we show that this
combined test is more powerful than either the continuous or dichotomous
component in isolation, or a t-test on the zero-inflated data. While developed
for measurements from a specific platform (Fluidigm), these tools are
generalizable to other multi-parametric measures over large numbers of events.Comment: 9 pages, 5 figure
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Transient cavitation and friction-induced heating effects of diesel fuel during the needle valve early opening stages for discharge pressures up to 450 mpa
An investigation of the fuel heating, vapor formation, and cavitation erosion location patterns inside a five-hole common rail diesel fuel injector, occurring during the early opening period of the needle valve (from 2 µm to 80 µm), discharging at pressures of up to 450 MPa, is presented. Numerical simulations were performed using the explicit density-based solver of the compressible Navier–Stokes (NS) and energy conservation equations. The flow solver was combined with tabulated property data for a four-component diesel fuel surrogate, derived from the perturbed chain statistical associating fluid theory (PC-SAFT) equation of state (EoS), which allowed for a significant amount of the fuel’s physical and transport properties to be quantified. The Wall Adapting Local Eddy viscosity (WALE) Large Eddy Simulation (LES) model was used to resolve sub-grid scale turbulence, while a cell-based mesh deformation arbitrary Lagrangian–Eulerian (ALE) formulation was used for modelling the injector’s needle valve movement. Friction-induced heating was found to increase significantly when decreasing the pressure. At the same time, the Joule–Thomson cooling effect was calculated for up to 25 degrees K for the local fuel temperature drop relative to the fuel’s feed temperature. The extreme injection pressures induced fuel jet velocities in the order of 1100 m/s, affecting the formation of coherent vortical flow structures into the nozzle’s sac volume
A Search for Intrinsic Polarization in O Stars with Variable Winds
New observations of 9 of the brightest northern O stars have been made with
the Breger polarimeter on the 0.9~m telescope at McDonald Observatory and the
AnyPol polarimeter on the 0.4~m telescope at Limber Observatory, using the
Johnson-Cousins UBVRI broadband filter system. Comparison with earlier
measurements shows no clearly defined long-term polarization variability. For
all 9 stars the wavelength dependence of the degree of polarization in the
optical range can be fit by a normal interstellar polarization law. The
polarization position angles are practically constant with wavelength and are
consistent with those of neighboring stars. Thus the simplest conclusion is
that the polarization of all the program stars is primarily interstellar.
The O stars chosen for this study are generally known from ultraviolet and
optical spectroscopy to have substantial mass loss rates and variable winds, as
well as occasional circumstellar emission. Their lack of intrinsic polarization
in comparison with the similar Be stars may be explained by the dominance of
radiation as a wind driving force due to higher luminosity, which results in
lower density and less rotational flattening in the electron scattering inner
envelopes where the polarization is produced. However, time series of
polarization measurements taken simultaneously with H-alpha and UV spectroscopy
during several coordinated multiwavelength campaigns suggest two cases of
possible small-amplitude, periodic short-term polarization variability, and
therefore intrinsic polarization, which may be correlated with the more widely
recognized spectroscopic variations.Comment: LaTeX2e, 22 pages including 11 tables; 12 separate gif figures; uses
aastex.cls preprint package; accepted by The Astronomical Journa
MAST: a flexible statistical framework for assessing transcriptional changes and characterizing heterogeneity in single-cell RNA sequencing data
Single-cell transcriptomics reveals gene expression heterogeneity but suffers from stochastic dropout and characteristic bimodal expression distributions in which expression is either strongly non-zero or non-detectable. We propose a two-part, generalized linear model for such bimodal data that parameterizes both of these features. We argue that the cellular detection rate, the fraction of genes expressed in a cell, should be adjusted for as a source of nuisance variation. Our model provides gene set enrichment analysis tailored to single-cell data. It provides insights into how networks of co-expressed genes evolve across an experimental treatment. MAST is available at https://github.com/RGLab/MAST
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String cavitation formation inside fuel injectors
The formation of vortex or 'string' cavitation has been visualised at pressures up to 2000 bar in an automotive-sized optical diesel fuel injector nozzle. The multi-hole nozzle geometry studied allowed observation of the hole-to-hole vortex interaction and, in particular, that of a bridging vortex in the sac region between the holes. Above a threshold Reynolds number, their formation and appearance during a 2 ms injection event was repeatable and independent of upstream pressure and cavitation number. In addition, two different hole layouts and threedimensional flow simulations have been employed to describe how, the relative positions of adjacent holes influenced the formation and hole-to-hole interaction of the observed string cavitation vortices, with good agreement between the experimental and simulation results being achieved
Interstellar polarization and grain alignment: the role of iron and silicon
We compiled the polarimetric data for a sample of lines of sight with known
abundances of Mg, Si, and Fe. We correlated the degree of interstellar
polarization and polarization efficiency (the ratio of to the colour
excess or extinction ) with dust phase abundances. We detect an
anticorrelation between and the dust phase abundance of iron in non
silicate - containing grains ]_\rm d, a correlation
between and the abundance of Si, and no correlation between or
and dust phase abundances. These findings can be explained if mainly
the silicate grains aligned by the radiative mechanism are responsible for the
observed interstellar linear polarization.Comment: Accepted for publication in Astronomy and Astrophysic
Towards an understanding of the Of?p star HD 191612: optical spectroscopy
We present extensive optical spectroscopy of the early-type magnetic star HD
191612 (O6.5f?pe-O8fp). The Balmer and HeI lines show strongly variable
emission which is highly reproducible on a well-determined 538-d period. Metal
lines and HeII absorptions (including many selective emission lines but
excluding He II 4686A emission) are essentially constant in line strength, but
are variable in velocity, establishing a double-lined binary orbit with P(orb)
= 1542d, e=0.45. We conduct a model-atmosphere analysis of the primary, and
find that the system is consistent with a O8: giant with a B1: main-sequence
secondary. Since the periodic 538-d changes are unrelated to orbital motion,
rotational modulation of a magnetically constrained plasma is strongly favoured
as the most likely underlying `clock'. An upper limit on the equatorial
rotation is consistent with this hypothesis, but is too weak to provide a
strong constraint.Comment: Accepted for MNRA
Epidemiology of HIV Infection in Large Urban Areas in the United States
Background: While the U.S. HIV epidemic continues to be primarily concentrated in urban area, local epidemiologic profiles may differ and require different approaches in prevention and treatment efforts. We describe the epidemiology of HIV in large urban areas with the highest HIV burden. Methods/Principal Findings: We used data from national HIV surveillance for 12 metropolitan statistical areas (MSAs) to determine disparities in HIV diagnoses and prevalence and changes over time. Overall, 0.3 % to 1 % of the MSA populations were living with HIV at the end of 2007. In each MSA, prevalence was.1 % among blacks; prevalence was.2 % in Miami, New York, and Baltimore. Among Hispanics, prevalence was.1 % in New York and Philadelphia. The relative percentage differences in 2007 HIV diagnosis rates, compared to whites, ranged from 239 (San Francisco) to 1239 (Baltimore) for blacks and from 15 (Miami) to 413 (Philadelphia) for Hispanics. The epidemic remains concentrated, with more than 50 % of HIV diagnoses in 2007 attributed to male-to-male sexual contact in 7 of the 12 MSAs; heterosexual transmission surpassed or equaled male-to-male sexual transmission in Baltimore, Philadelphia, and Washington, DC. Yet in several MSAs, including Baltimore and Washington, DC, AIDS diagnoses increased among men-who-have sex with men in recent years. Conclusions/Significance: These data are useful to identify local drivers of the epidemic and to tailor public health effort
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On the formation of string cavitation inside fuel injectors
The formation of vortex or ‘string’ cavitation has been visualised in the flow upstream of the injection hole inlet of an automotive-sized optical diesel fuel injector nozzle operating at pressures up to 2,000 bar. Three different nozzle geometries and three-dimensional flow simulations have been employed to describe how, for two adjacent nozzle holes, their relative positions influenced the formation and hole-to-hole interaction of the observed string cavitation vortices. Each hole was shown to contain two counter-rotating vortices: the first extending upstream on axis with the nozzle hole into the nozzle sac volume and the second forming a single ‘bridging’ string linked to the adjacent hole. Steady-state and transient fuel injection conditions were shown to produce significantly different nozzle-flow characteristics with regard to the formation and interaction of these vortices in the geometries tested, with good agreement between the experimental and simulation results being achieved. The study further confirms that the visualised vortices do not cavitate themselves but act as carriers of gas-phase components within the injector flow
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