554 research outputs found
Origin of an apparent B chromosome by mutation, chromosome fragmentation and specific DNA sequence amplification
Absolute frequency measurement of the magnesium intercombination transition
We report on a frequency measurement of the clock
transition of Mg on a thermal atomic beam. The intercombination
transition has been referenced to a portable primary Cs frequency standard with
the help of a femtosecond fiber laser frequency comb. The achieved uncertainty
is which corresponds to an increase in accuracy of six
orders of magnitude compared to previous results. The measured frequency value
permits the calculation of several other optical transitions from to
the -level system for Mg, Mg and Mg. We describe in
detail the components of our optical frequency standard like the stabilized
spectroscopy laser, the atomic beam apparatus used for Ramsey-Bord\'e
interferometry and the frequency comb generator and discuss the uncertainty
contributions to our measurement including the first and second order Doppler
effect. An upper limit of in one second for the short term
instability of our optical frequency standard was determined by comparison with
a GPS disciplined quartz oscillator.Comment: 8 pages, 8 figure
Long-distance remote comparison of ultrastable optical frequencies with 1e-15 instability in fractions of a second
We demonstrate a fully optical, long-distance remote comparison of
independent ultrastable optical frequencies reaching a short term stability
that is superior to any reported remote comparison of optical frequencies. We
use two ultrastable lasers, which are separated by a geographical distance of
more than 50 km, and compare them via a 73 km long phase-stabilized fiber in a
commercial telecommunication network. The remote characterization spans more
than one optical octave and reaches a fractional frequency instability between
the independent ultrastable laser systems of 3e-15 in 0.1 s. The achieved
performance at 100 ms represents an improvement by one order of magnitude to
any previously reported remote comparison of optical frequencies and enables
future remote dissemination of the stability of 100 mHz linewidth lasers within
seconds.Comment: 7 pages, 4 figure
-BaBO deep UV monolithic walk-off compensating tandem
The generation of watt-level cw narrow-linewidth sources at specific deep UV
wavelengths corresponding to atomic cooling transitions usually employs
external cavity-enhanced second-harmonic generation (SHG) of moderate-power
visible lasers in birefringent materials. In this work, we investigate a novel
approach to cw deep-UV generation by employing the low-loss BBO in a monolithic
walkoff-compensating structure [Zondy {\it{et al}}, J. Opt. Soc. Am. B
{\bf{20}} (2003) 1675] to simultaneously enhance the effective nonlinear
coefficient while minimizing the UV beam ellipticity under tight focusing. As a
preliminary step to cavity-enhanced operation, and in order to apprehend the
design difficulties stemming from the extremely low acceptance angle of BBO, we
investigate and analyze the single-pass performance of a mm monolithic
walk-off compensating structure made of 2 optically-contacted BBO plates cut
for type-I critically phase-matched SHG of a cw nm dye laser. As
compared with a bulk crystal of identical length, a sharp UV efficiency
enhancement factor of 1.65 has been evidenced with the tandem structure, but at
nm from the targeted fundamental wavelength, highlighting the
sensitivity of this technique when applied to a highly birefringent material
such as BBO. Solutions to angle cut residual errors are identified so as to
match accurately more complex periodic-tandem structure performance to any
target UV wavelength, opening the prospect for high-power, good beam quality
deep UV cw laser sources for atom cooling and trapping.Comment: 21 pages, 8 figures, to appear in Opt. Commu
Integrated fiber-mirror ion trap for strong ion-cavity coupling
We present and characterize fiber mirrors and a miniaturized ion-trap design developed to integrate a fiber-based Fabry-Perot cavity (FFPC) with a linear Paul trap for use in cavity-QED experiments with trapped ions. Our fiber-mirror fabrication process not only enables the construction of FFPCs with small mode volumes, but also allows us to minimize the influence of the dielectric fiber mirrors on the trapped-ion pseudopotential. We discuss the effect of clipping losses for long FFPCs and the effect of angular and lateral displacements on the coupling efficiencies between cavity and fiber. Optical profilometry allows us to determine the radii of curvature and ellipticities of the fiber mirrors. From finesse measurements, we infer a single-atom cooperativity of up to 12 for FFPCs longer than 200 μm in length; comparison to cavities constructed with reference substrate mirrors produced in the same coating run indicates that our FFPCs have similar scattering losses. We characterize the birefringence of our fiber mirrors, finding that careful fiber-mirror selection enables us to construct FFPCs with degenerate polarization modes. As FFPCs are novel devices, we describe procedures developed for handling, aligning, and cleaning them. We discuss experiments to anneal fiber mirrors and explore the influence of the atmosphere under which annealing occurs on coating losses, finding that annealing under vacuum increases the losses for our reference substrate mirrors. X-ray photoelectron spectroscopy measurements indicate that these losses may be attributable to oxygen depletion in the mirror coating. Special design considerations enable us to introduce a FFPC into a trapped ion setup. Our unique linear Paul trap design provides clearance for such a cavity and is miniaturized to shield trapped ions from the dielectric fiber mirrors. We numerically calculate the trap potential in the absence of fibers. In the experiment additional electrodes can be used to compensate distortions of the potential due to the fibers. Home-built fiber feedthroughs connect the FFPC to external optics, and an integrated nanopositioning system affords the possibility of retracting or realigning the cavity without breaking vacuum
Alien chromosome segment from Aegilops speltoides and Dasypyrum villosum increases drought tolerance in wheat via profuse and deep root system
BackgroundRecurrent drought associated with climate change is a major constraint to wheat (Triticum aestivum L.) productivity. This study aimed to (i) quantify the effects of addition/substitution/translocation of chromosome segments from wild relatives of wheat on the root, physiological and yield traits of hexaploid wheat under drought, and (ii) understand the mechanism(s) associated with drought tolerance or susceptibility in wheat-alien chromosome lines.MethodsA set of 48 wheat-alien chromosome lines (addition/substitution/translocation lines) with Chinese Spring background were used. Seedling root traits were studied on solid agar medium. To understand the influence of drought on the root system of adult plants, these 48 lines were grown in 150-cm columns for 65 d under full irrigation or withholding water for 58 d. To quantify the effect of drought on physiological and yield traits, the 48 lines were grown in pots under full irrigation until anthesis; after that, half of the plants were drought stressed by withholding water for 16 d before recording physiological and yield-associated traits.ResultsThe alien chromosome lines exhibited altered root architecture and decreased photochemical efficiency and seed yield and its components under drought. The wheat-alien chromosome lines T5DS5S#3L (TA5088) with a chromosome segment from Aegilops speltoides (5S) and T5DL(.)5V#3S (TA5638) with a chromosome segment from Dasypyrum villosum (5V) were identified as drought tolerant, and the drought tolerance mechanism was associated with a deep, thin and profuse root system.ConclusionsThe two germplasm lines (TA5088 and TA5638) could be used in wheat breeding programs to improve drought tolerance in wheat and understand the underlying molecular genetic mechanisms of root architecture and drought tolerance
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