Non-reversibility and self-joinings of higher orders for ergodic flows

By studying the weak closure of multidimensional off-diagonal self-joinings we provide a criterion for non-isomorphism of a flow with its inverse, hence the non-reversibility of a flow. This is applied to special flows over rigid automorphisms. In particular, we apply the criterion to special flows over irrational rotations, providing a large class of non-reversible flows, including some analytic reparametrizations of linear flows on the two torus, so called von Neumann's flows and some special flows with piecewise polynomial roof functions.. A topological counterpart is also developed with the full solution of the problem of the topological self-similarity of continuous special flows over irrational rotations. This yields examples of continuous special flows over irrational rotations without topological self-similarities and having all non-zero real numbers as scales of measure-theoretic self-similarities.Comment: 49 pages, 2 figur

Mild mixing property for special flows under piecewise constant functions

We give a condition on a piecewise constant roof function and an irrational rotation by $\alpha$ on the circle to give rise to a special flow having the mild mixing property. Such flows will also satisfy Ratner's property. As a consequence we obtain a class of mildly mixing singular flows on the two-torus that arise from quasi-periodic Hamiltonians flows by velocity changes.Comment: Accepted for publication in Discrete Contin. Dyn. Sys

Analysis of the microstructure evolution during thermo-mechanical treatment of the steel plates in grade X80-X100

In the work the results of the physical modeling of plate rolling process of HSLA steel were presented. The simulations were carried out using the Gleeble 3800 device and the anvils set for plane strain compression study. The aim of the simulation were a determination of a influence of changes in chemical composition of steel on obtained final structure and tensile strength of specimens after controlled deformation and accelerated cooling to room temperature. During investigation three grades of steels with a little different chemical composition were examined

The prospects for Yb- and Nd-doped tungstate microchip lasers

Potassium gadolinium and yttrium tungstates doped with trivalent Nd, Yb or Tm ions are widely used crystals for efficient diode-pumped lasers. They are usually oriented along the b crystallographic axis, which is also the N p axis of optical indicatrix. The Np-cut is characterized by a strong thermal lens with opposing signs along Nm and N g [1]. This is unsuited to microchip lasers which require a positive thermal lens for a stable cavity. Therefore, a detailed investigation of thermo-optic effects and microchip laser potential of other crystallographic orientations in tungstate crystals is of interest

Author Correction: New paradigms for understanding and step changes in treating active and chronic, persistent apicomplexan infections

Correction to: Scientific Reports https://doi.org/10.1038/srep29179, published online 14 July 201

New paradigms for understanding and step changes in treating active and chronic, persistent apicomplexan infections

Toxoplasma gondii, the most common parasitic infection of human brain and eye, persists across lifetimes, can progressively damage sight, and is currently incurable. New, curative medicines are needed urgently. Herein, we develop novel models to facilitate drug development: EGS strain T. gondii forms cysts in vitro that induce oocysts in cats, the gold standard criterion for cysts. These cysts highly express cytochrome b. Using these models, we envisioned, and then created, novel 4-(1H)-quinolone scaffolds that target the cytochrome bc1 complex Qi site, of which, a substituted 5,6,7,8-tetrahydroquinolin-4-one inhibits active infection (IC50, 30 nM) and cysts (IC50, 4 μM) in vitro, and in vivo (25 mg/kg), and drug resistant Plasmodium falciparum (IC50, <30 nM), with clinically relevant synergy. Mutant yeast and co-crystallographic studies demonstrate binding to the bc1 complex Qi site. Our results have direct impact on improving outcomes for those with toxoplasmosis, malaria, and ~2 billion persons chronically infected with encysted bradyzoites

Comparative proteomic analysis of spermatozoa isolated by swim-up or density gradient centrifugation

Abstract BACKGROUND: Reports about the morphologic and functional characteristics of spermatozoa prepared by density gradient centrifugation (DC) or swim-up (SU) have produced discordant results. We have performed a proteomic comparison of cells prepared by DC and SU providing a molecular insight into the differences between these two methods of sperm cell isolation. METHODS: Protein maps were obtained by 2-dimensional (2-D) separations consisting of isoelectrofocusing (IEF) from pI 3 to 11 followed by SDS-polyacrylamide gel electrophoresis. 2-D gels were stained with Sypro Ruby. Map images of DC and SU spermatozoa were compared using dedicated software. Intensities of a given spot were considered different between DC and SU when their group mean differed by >1.5-fold (p<0.05, Anova). RESULTS: No differences were observed for 853 spots, indicating a 98.7% similarity between DC and SU. Five spots were DC>SU and 1 was SU>DC. Proteins present in 3 of the differential spots could be identified. One DC>SU spot contained lactate dehydrogenase C and gamma-glutamylhydrolase, a second DC>SU spot contained fumarate hydratase and glyceraldehyde-3-phosphate dehydrogenase-2, and a SU>DC spot contained pyruvate kinase M1/M2. CONCLUSIONS: The differences in protein levels found on comparison of DC with SU spermatozoa indicate possible dissimilarities in their glycolytic metabolism and DNA methylation and suggest that DC cells may have a better capacitation potential

A proteomic approach to investigating gene cluster expression and secondary metabolite functionality in Aspergillus fumigatus.

A combined proteomics and metabolomics approach was utilised to advance the identification and characterisation of secondary metabolites in Aspergillus fumigatus. Here, implementation of a shotgun proteomic strategy led to the identification of non-redundant mycelial proteins (n = 414) from A. fumigatus including proteins typically under-represented in 2-D proteome maps: proteins with multiple transmembrane regions, hydrophobic proteins and proteins with extremes of molecular mass and pI. Indirect identification of secondary metabolite cluster expression was also achieved, with proteins (n = 18) from LaeA-regulated clusters detected, including GliT encoded within the gliotoxin biosynthetic cluster. Biochemical analysis then revealed that gliotoxin significantly attenuates H2O2-induced oxidative stress in A. fumigatus (p>0.0001), confirming observations from proteomics data. A complementary 2-D/LC-MS/MS approach further elucidated significantly increased abundance (p<0.05) of proliferating cell nuclear antigen (PCNA), NADH-quinone oxidoreductase and the gliotoxin oxidoreductase GliT, along with significantly attenuated abundance (p<0.05) of a heat shock protein, an oxidative stress protein and an autolysis-associated chitinase, when gliotoxin and H2O2 were present, compared to H2O2 alone. Moreover, gliotoxin exposure significantly reduced the abundance of selected proteins (p<0.05) involved in de novo purine biosynthesis. Significantly elevated abundance (p<0.05) of a key enzyme, xanthine-guanine phosphoribosyl transferase Xpt1, utilised in purine salvage, was observed in the presence of H2O2 and gliotoxin. This work provides new insights into the A. fumigatus proteome and experimental strategies, plus mechanistic data pertaining to gliotoxin functionality in the organism