New concept for a regenerative and resorbable prosthesis for tendon and ligament. Physicochemical and biological characterization of PLA-braided biomaterial

We present a concept for a new regenerative and resorbable prosthesis for tendon and ligament and characterize the physicomechanical and biological behavior of one of its components, a hollow braid made of poly-lactide acid (PLA) which is the load-bearing part of the prosthesis concept. The prosthesis consists of a braid, microparticles in its interior serving as cell carriers, and a surface non-adherent coating, all these parts being made of biodegradable materials. The PLA braid has a nonlinear convex stress-strain behavior with a Young modulus of 1370 +/- 90 MPa in the linear, stretched state, and after 12 months of hydrolytic degradation the modulus shows a reduction by a factor of four. Different disinfection methods were tested as to their efficiency in cleansing the braid and preparing it for cell culture. Fibroblasts of L929 line were grown on the PLA braid for 14 days, showing good adherence and proliferation. These studies validate the PLA braid for the intended purpose in the regenerative prosthesis concept. (c) 2013 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 101A: 3228-3237, 2013This work has been developed thanks to the financial support of AITEX (Valencia, Spain). JME thanks Drs. Isabel Pascual, Andres Pena, and their team from Hospital Clinico of Valencia for their fine work.Araque Monrós, MC.; Gamboa Martinez, TC.; Gil Santos, L.; Gironés Bernabé, S.; Monleón Pradas, M.; Más Estellés, J. (2013). New concept for a regenerative and resorbable prosthesis for tendon and ligament. Physicochemical and biological characterization of PLA-braided biomaterial. Journal of Biomedical Materials Research Part A. 101A(11):3228-3237. doi:10.1002/jbm.a.34633S32283237101A11Vieira, A. C., Guedes, R. M., & Marques, A. T. (2009). Development of ligament tissue biodegradable devices: A review. Journal of Biomechanics, 42(15), 2421-2430. doi:10.1016/j.jbiomech.2009.07.019Kuo, C. K., Marturano, J. 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Mechanics rules cell biology

Cells in the musculoskeletal system are subjected to various mechanical forces in vivo. Years of research have shown that these mechanical forces, including tension and compression, greatly influence various cellular functions such as gene expression, cell proliferation and differentiation, and secretion of matrix proteins. Cells also use mechanotransduction mechanisms to convert mechanical signals into a cascade of cellular and molecular events. This mini-review provides an overview of cell mechanobiology to highlight the notion that mechanics, mainly in the form of mechanical forces, dictates cell behaviors in terms of both cellular mechanobiological responses and mechanotransduction

SBC2007-175814 EFFECT OF MECHANICAL STIMULATION ON THE BIOMECHANICS OF STEM CELL - COLLAGEN SPONGE CONSTRUCTS FOR PATELLAR TENDON REPAIR

INTRODUCTION Tendons (rotator cuff, Achilles and patellar tendons) are among the most commonly injured soft tissues This study was undertaken to determine how mechanical stimulation in culture of MSC-type I collagen sponge constructs affects the biomechanics and histology of rabbit patellar tendon (PT) defect repairs 12 weeks after surgery. The hypotheses to be tested were that mechanical stimulation would improve: 1) the linear stiffness and linear modulus of the in vitro construct after 2 weeks in culture, and 2) repair biomechanics and histological appearance 12 weeks after implantation. 3) We also hypothesized that the construct and repair stiffness and modulus would be positively correlated, suggesting that the in vitro measure might be a predictor of in vivo repair outcome