Genome-wide computational identification of WG/GW Argonaute-binding proteins in Arabidopsis

Domains in Arabidopsis proteins NRPE1 and SPT5-like, composed almost exclusively of repeated motifs in which only WG or GW sequences and an overall amino-acid preference are conserved, have been experimentally shown to bind multiple molecules of Argonaute (AGO) protein(s). Domain swapping between the WG/GW domains of NRPE1 and the human protein GW182 showed a conserved function. As classical sequence alignment methods are poorly-adapted to detect such weakly-conserved motifs, we have developed a tool to carry out a systematic analysis to identify genes potentially encoding AGO-binding GW/WG proteins. Here, we describe exhaustive analysis of the Arabidopsis genome for all regions potentially encoding proteins bearing WG/GW motifs and consider the possible role of some of them in AGO-dependent mechanisms. We identified 20 different candidate WG/GW genes, encoding proteins in which the predicted domains range from 92aa to 654aa. These mostly correspond to a limited number of families: RNA-binding proteins, transcription factors, glycine-rich proteins, translation initiation factors and known silencing-associated proteins such as SDE3. Recent studies have argued that the interaction between WG/GW-rich domains and AGO proteins is evolutionarily conserved. Here, we demonstrate by an in silico domain-swapping simulation between plant and mammalian WG/GW proteins that the biased amino-acid composition of the AGO-binding sites is conserved

Do catálogo musicológico ao corpus digital em devir: Dezède, história e cronologia dos espetáculos

Communication parue dans des actes sous forme d'une édition sur cédérom, 38568 caractères.International audienc

Lorsque le cirque dialogue avec le territoire : des médiations hybrides aux médiations circulaires

International audienceSecteur artistique en quête perpétuelle de formes nouvelles, désireux de se démarquer du cirque traditionnel, art hybride, polymorphe et novateur, qui résiste à toute tentative de définition, tant dans sa dimension esthétique que dans la démarche artistique qu'il met en oeuvre, le cirque contemporain a trouvé à Auch une structure à sa mesure. Ce chef-lieu de département rural, ville moyenne de 23000 habitants, abrite et soutient l'association CIRCa-centre d'innovation et de recherche circassiens-structure originale qui a mis à profit des collaborations de tous ordres (public-privé, amateurs-professionnels), portées par une ambition à la fois économique, sociale et culturelle, pour devenir un modèle de gestion et de développement innovant de la culture dans les territoires. Notre propos est d'étudier les médiations culturelles portées par une structure telle que CIRCa, pour analyser comment un acteur culturel peut tenter de sensibiliser des publics et des populations à la création artistique, en portant un projet de territoire. Comment un acteur culturel se mue-t-il en acteur du territoire, par le biais de projets culturels structurants en bousculant les cadres du triptyque classique production/diffusion/médiation 1 ? Notre propos sera porté par une double approche mêlant arts du spectacle et sciences de l'information et la communication

Comparison of pre- and inter-stage aerobic treatment of wastewater sludge: effects on biogas production and COD removal

The aim of this study was to investigate thermophilic (55 ° C) aerobic digestion (TAD) as pre- and inter-stage treatment of sludge anaerobic digestion and to analyse the change in organic matter accessibility and complexity. Pre-treatment decreased methane yield (up to -70%), due to oxidation losses whereas inter-stage treatment slightly improved overall methane yield (+2.6%) and total COD removal (+5%) compared to control. Anaerobic degradability and COD removal in the second anaerobic stage significantly increased, by 13 to 40%. Organic matter fractionation showed that TAD led to an increase in sludge organic matter accessibility in all cases. Organic matter complexity, measured by fluorimetry, increased after TAD pre-treatment whereas it remained constant after inter-stage treatment. TAD was shown to be more efficient if applied to a more recalcitrant substrate and should thus be used as inter-stage treatment to avoid decreasing methane production

Preliminary data on a new early Pre-Pottery Neolithic site on Cyprus (Ayios Tychonas - Klimonas, ca. 9000 cal. BC)

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Methods to Assess Biological Transformation of Biomass

The aim of this chapter is to provide a description of the main methods used to assess biological transformation of biomass. It will address saccharification tests which account for the ability of biomass to release sugars during enzymatic hydrolysis. Biochemical methane potential (BMP) tests, which are widely used to assess anaerobic digestibility of biomass for the production of methane and BMP prediction by near infra-red spectroscopy will be presented. Biohydrogen potential (BHP), used to assess the ability to produce biohydrogen from biomass by dark fermentation will be described. Respirometry tests accounting for assessing aerobic degradability of biomass will be described. Incubation tests used to assess carbon and nitrogen degradation in soils will also be presented

Conditional Probability of Survival and Prognostic Factors in Long-Term Survivors of High-Grade Serous Ovarian Cancer

International audienceObjective: High-grade serous ovarian cancers (HGSOC) are heterogeneous, often diagnosed at an advanced stage, and associated with poor overall survival (OS, 39% at five years). There are few data about the prognostic factors of late relapses in HGSOC patients who survived ≥five years, long-term survivors (LTS). The aim of our study is to assess the probability of survival according to the already survived time from diagnosis. Methods: Data from HGSOC patients treated between 1995 and 2016 were retrospectively collected to estimate the conditional probability of survival (CPS), probability of surviving Y years after diagnosis when the patient had already survived X years, and to determine the LTS prognostic factors. The primary endpoint was OS. Results: 404 patients were included; 120 of them were LTS. Patients were aged 61 years (range: 20–89), WHO performance status 0–1 in 86.9% and 2 in 13.1%, and Fédération Internationale de Gynécologie et d’Obstétrique (FIGO) staging III and IV in 82.7% and 17.3% patients. Breast cancer (BRCA) status was available in 116 patients (33% mutated), including 58 LTS (36% mutated). No macroscopic residual disease was observed in 58.4% patients. First-line platinum-based chemotherapy plus paclitaxel was administered in 80.4% of patients (median: six cycles (range: 1–14)). After a 9 point 3-year follow-up, median OS was four years (95% CI: 3.6–4.5). The CPS at five years after surviving one year was 42.8% (95% CI: 35.3–48.3); it increased to 81.7% (95% CI: 75.5–87.8) after four survived years. Progression-free interval>18 months was the only LTS prognostic factor in the multivariable analysis (hazard ratio (HR) = 0.23; 95% CI: 0.13–0.40; p < 0.001). Conclusion: The CPS provided relevant and encouraging clinical information on the life expectancy of HGSOC patients who already survived a period of time after diagnosis. LTS prognostic factors are useful for clinicians and patients