modelling the combined use of bicycles and train in Lisbon, Portugal

UIDB/04647/2020 UIDP/04647/2020 UIDB/03127/2020Many capital cities are expanding bicycle lanes or investing in public bike-sharing systems to make cycling mobility a viable transport solution within urban areas. While bicycles are fit for short-distance trips (publishersversionepub_ahead_of_prin

Speech features for discriminating stress using branch and bound wrapper search

Stress detection from speech is a less explored field than Automatic Emotion Recognition and it is still not clear which features are better stress discriminants. VOCE aims at doing speech classification as stressed or not-stressed in real-time, using acoustic-prosodic features only. We therefore look for the best discriminating feature subsets from a set of 6285 features – 6125 features extracted with openSMILE toolkit and 160 Teager Energy Operator (TEO) features. We use a mutual information filter and a branch and bound wrapper heuristic with an SVM classifier to perform feature selection. Since many feature sets are selected, we analyse them in terms of chosen features and classifier performance concerning also true positive and false positive rates. The results show that the best feature types for our application case are Audio Spectral, MFCC, PCM and TEO. We reached results as high as 70.36% for generalisation accuracyinfo:eu-repo/semantics/acceptedVersio

Efficacy of dignity therapy for depression and anxiety in terminally-ill patients: early results of a randomized controlled trial

Objective: Dignity therapy (DT) is a short-term psychotherapy developed for patients living with a life-limiting illness. Our aim was to determine the influence of DT on symptoms of depression and anxiety in people with a life-threatening disease with high level of distress, referred to an inpatient palliative care unit. Method: This was an open-label randomized controlled trial. Sixty terminally ill patients were randomly assigned to one of two groups: intervention group (DT+ standard palliative care [SPC]) or control group (SPC alone). The main outcomes were symptoms of depression and anxiety, measured with the Hospital Anxiety and Depression Scale, assessed at baseline, day 4, day 15, and day 30 of follow-up. Results: Of the 60 participants, 29 were randomized to DT and 31 to SPC. Baseline characteristics were similar between the two groups. DT was associated with a significant decrease in depressive symptoms at day 4 and day 15 (mean = −4.46, 95% CI, −6.91–2.02, p = 0.001; mean= −3.96, 95% CI, −7.33 to −0.61; p = 0.022, respectively), but not at day 30 (mean = −3.33, 95% CI, −7.32–0.65, p = 0.097). DT was also associated with a significant decrease in anxiety symptoms at each follow-up (mean= −3.96, 95% CI, −6.66 to −1.25, p = 0.005; mean= −6.19, 95% CI, −10.49 to −1.88, p = 0.006; mean = −5.07, 95% CI, −10.22 to −0.09, p = 0.054, respectively). Significance of results: DT appears to have a short-term beneficial effect on the depression and anxiety symptoms that often accompany patients at the end of their lives. Future research with larger samples compared with other treatments is needed to better understand the potential benefits of this psychotherapy

Rippikoulukirjojen seksuaalikasvatuksen ja -opetuksen tavoitteet

Tutkitaan Rippikouluoppaan IV sekä Elämä – usko – rukous – Rippikoulusuunnitelman 2001 valossa, kuinka Rippikoulun opettajien oppaissa on hyödynnetty edellä mainittujen linjauksia ja toimintaideoita. Tutkimuksessa tarkastellaan, mitä Rippikouluopas IV sekä Elämä – usko – rukous – Rippikoulusuunnitelma 2001 pitää tärkeänä tavoitteena seksuaalikasvatuksen opetuksessa. Tutkitaan, onko rippikoulun ohjaajan kirjoista löydettävissä sama tavoitesuuntaus, jotka Rippikouluoppaan IV ja Elämä – usko – rukous – Rippikoulusuunnitelma 2001 pitävät sisällään. Rippikouluopas IV nostaa esiin kolme tärkeää asiaa, jotka seksuaalikasvatuksessa erityisesti tulee nostaa esiin. Se opastaa avaamaan käsitteitä, jotta nuori tietää, mitä seksuaalisuutta koskevalla sanalla oikeasti tarkoitetaan. Seksuaalikasvatuksen tulee olla myös foorumi tunteiden käsittelemiselle. Ryhmän tulisi olla turvallinen jokaiselle rippikoululaiselle, jotta jokainen uskaltaisi olla oma itsensä. Kolmantena nostetaan esiin seksuaalisuuden pyhyys. Elämä – usko – rukous – rippikoulusuunnitelma 2001 nostaa esiin kolme koria ja jokaisella oppitunnilla tulisi hyödyntää elämää – uskoa ja rukousta.In this thesis I will study the guidelines of the Confirmation Class Textbook IV (Rippikouluopas IV) and the Confirmation Class Plan 2001, Life - Faith - Prayer (Rippikoulusuunnitelma 2001, Elämä - usko - rukous). I will study if the Confirmation Class Teachers' Handbook emphasizes these guidelines. I will research what the Confirmation Class Textbook IV and the Confirmation Class Plan 2001 consider their main aims in sex education. I will then study if the Teachers' Handbook makes use of the same guiding principles.The Confirmation Class Textbook IV emphasizes three aspects of sex education. Firstly, It points out the importance of analysing concepts as this will help young people to understand the real meaning of vocabulary used in sex education. Secondly, according to the Textbook IV, sex education should be a forum for dealing with emotions. The group should be safe for every con-firmand and encourage them to be themselves. Thirdly, the sacredness of sexuality is empha-sized.The Confirmation Class Plan 2001 emphasizes three different themes. Every Confirmation Class should make use of life, faith and prayer

Partial purification and thermal stability of two peroxidases from Pithecellobium dulce (Roxb.) Benth. Aril.

Abstract: Two peroxidase (POX) forms, named PdPI and PdPII, were partially purified from the Pithecellobium dulce aril, using ammonium sulfate precipitation and anion exchange chromatography. Zymography of the proteins obtained after precipitation in the range of 60-90% ammonium sulfate (F6090 fraction) showed two bands with peroxidase activity. In DE-52 cellulose column, the peroxidase activity was detected in unadsorbed peak (PdPI) and adsorbed peak eluted with 0.2 mol L-1 NaCl (PdPII) suggesting that PdPI (~114 KDa) and PdPII (~77 KDa) are basic and acidic proteins, respectively, being PdPI stable at 80 ºC. This is the first report of partial purification of POX from P. dulce aril, indicating that this species may be a new source of enzymes for use in biosensors and other biotechnological applications. [Purificação Parcial e Estabilidade Térmica de Duas Peroxidases Extraídas de Arilos de Pithecellobium dulce (Roxb.) Benth]. Resumo: Duas formas de peroxidase (POX), denominadas PdPI e PdPII, foram parcialmente purificadas de arilos de Pithecellobium dulce, utilizando precipitação com sulfato de amônio e cromatografia de troca iônica. O zimograma das proteínas obtidas após precipitação com sulfato de amônio na faixa de 60 a 90% de saturação (fração F6090), mostrou duas bandas com atividade peroxidásica. Na coluna de celulose DE-52, a atividade peroxidásica foi detectada nos picos não adsorvido (PdPI) e adsorvido (PdPII) eluído com NaCl 0,2 mol L-1, sugerindo que a PdPI (~114 KDa) e a PdPII (~77 KDa) são proteínas básicas e ácidas, respectivamente, sendo a PdPI estável a 80 ºC. Este é o primeiro relato de purificação parcial de POX obtida de arilos de P. dulce, indicando que esta espécie pode ser uma nova fonte de enzimas para utilização em biossensores e outras aplicações biotecnológicas.[HEVILA OLIVEIRA SALLES FIGUEIREDO]

What we learned in kindergarten: five tips for collaboration in oncology

As you read the five tenets presented here, think about these simple truths of leading and influencing others, managing failure, thinking strategically, and resolving conflicts. Ap - ply them to the world in which we all now live and play. Far too often work (the place) is viewed simply as where work (the action) occurs. What we are saying is that, although institutional targets (such as reducing wait times to see new patients) are all laudable goals, there has to be more, and play has to become an essential component of work. What can we uncover, rediscover, and create to make the time spent with one another the best possible experience for everyone involved? Even more importantly, what must we do to ensure that what we create and share has the possibil - ity and potential to make our lives and the world a better place? Play isn’t something we do as a part of life—it is life.info:eu-repo/semantics/publishedVersio

USPIO-enhanced magnetic resonance imaging for nodal staging in patients with head and neck cancer

PURPOSE: To determine the accuracy of ultrasmall superparamagnetic iron oxide (USPIO)-enhanced magnetic resonance imaging (MRI) for nodal staging in patients with head and neck cancer. MATERIALS AND METHODS: Twenty patients with carcinomas of the upper aerodigestive tract were prospectively enrolled. MRI was performed before and 24-36 hours after intravenous infusion of an USPIO agent, ferumoxtran-10 (Sinerem; Guerbet, France; and Combidex; Advanced Magnetics) at a dose of 2.6 mg Fe/kg using T2-weighted spin-echo and gradient-echo sequences. Surgery was performed the same day or the day after the ferumoxtran-10-enhanced MR examination. Based on MRI, selected nodes were surgically removed and directly correlated with pathology using hematoxylin-eosin (H&E) and Perls stainings. RESULTS: A total of 63 nodes were studied; 36 were nonmetastatic, 25 metastatic, and two inflammatory. Ferumoxtran-10-enhanced MRI allowed diagnosis of 24 metastatic and 30 nonmetastatic nodes, yielding a sensitivity of 96%, a specificity of 78.9%, a positive predictive value of 75%, and a negative predictive value of 96.8%, compared to 64%, 78.9%, 66.6%, and 76.9%, respectively, for nonenhanced MRI. Accuracy of ferumoxtran-10-enhanced MRI was 85.7%. The gradient-echo T2-weighted sequence was the most accurate to detect signal loss in nonmetastatic nodes. CONCLUSION: USPIO-enhanced MRI is useful for nodal staging of patients with head and neck cancer

Determinação da atividade de proteases cisteínicas, peroxidases e inibidores de papaína em sementes de Crotalaria spectabilis.

Vegetais são uma rica fonte de proteínas bioativas de amplo interesse e variadas aplicações. Proteases, peroxidases e inibidores de proteases são foco de diversas pesquisas no intuito de investigar a funcionalidade e uso dessas biomoléculas em diversas áreas. Proteases cisteínicas são importantes para o desenvolvimento das plantas, participando em processos como a mobilização de proteínas de reserva, senescência e morte celular programada (HAQ, 2004). Peroxidases ocorrem amplamente em vegetais e estão envolvidas em vários processos do metabolismo, podendo ser empregadas na construção de biosensores e em muitos processos industriais (FATIBELO e VIEIRA, 2002). Proteínas inibidoras de proteases são moléculas que inibem a atividade de enzimas proteolíticas participando nos processos de regulação endógena e na defesa das plantas (HAQ, 2004). Crotalaria spectabilis é uma leguminosa de ampla ocorrência no Ceará, sendo conhecida por sua resistência a nematóides fitopatogênicos (WANG, 2002). Este trabalho teve como objetivos detectar e quantificar enzimas proteolíticas, peroxidases e inibidores de proteases cisteínicas em sementes de Crotalaria spectabilis