Ecología térmica de Microlophus occipitalis (Sauria: Tropiduridae) en el Bosque Seco de Llanura de Tumbes, Perú

The thermal ecology of Microlophus occipitalis Peters 1871 in the plain dry forests of Tumbes (northewestern Peru) was studied. Mean body temperature was 36.1 ± 1.8 ºC, similar to body temperatures exposed by Microlophus peruvianus in northern Peru. There were no differences between body temperature and degree of thermoregulation of males and females, due to a possible association to their social structure and microhabitat use. Air and substrate temperature affects the body temperature of Microlophus occipitalis, where air temperature accounts for a significant proportion of body temperature variation. We suggest more detailed studies on this lizard species, especially under climate change scenarios in northwestern Peru.Se estudió la ecología termal de Microlophus occipitalis Peters 1871 en el Bosque Seco de Llanura de Tumbes (noroeste del Perú). La temperatura corporal promedio fue de 36,1 ± 1,8 ºC, similar a las temperaturas exhibidas por Microlophus peruvianus en el norte del Perú. No se identificaron diferencias entre la temperatura corporal y el grado de termorregulación de hembras y machos, posiblemente asociado a su estructura social y uso de microhábitat. La temperatura del aire y del sustrato afectaron la temperatura corporal de Microlophus occipitalis, aunque la temperatura del aire afecta en mayor grado la variación de la temperatura corporal. Se sugiere realizar estudios más detallados en esta especie, especialmente bajo escenarios de cambio climático en el noroeste del Perú

Diseño y validación analítica de una PCR duplex para la detección de Ehrlichia y Rickettsia en garrapatas

ABSTRACT: Ehrlichia and Rickettsia are two major rickettsial genera transmitted by ticks that affect a number of wild and domestic animal species and human populations around the world. Objective: To design and validate a duplex PCR for Ehrlichia and Rickettsia in ticks. Methods:Assay validation included testing for sensitivity,specificity, reproducibility, and robustness of the PCR. The groELand 23sr RNAgenes were used for Ehrlichia and Rickettsia, respectively. Results: The limit of detection was one hundred gene copies per 50 μLof reaction for Ehrlichia spp, and one gene copy of Rickettsia per 50 μL of reaction. In general, the primers of the test only amplified in silico those bacterial agents for which they were originally designed, with the exception of the primers for Rickettsia that also amplified Methylocystis sp. The test was reproducible (intermediate precision) 96.7% of the times for both agents. The test was robust enough to tolerate concentration changes of all reagents with the exception of Taq DNA polymerase. Conclusions: The validation results indicated that this PCR is useful for detection in both bacterial genera and it is a good candidate for diagnostic validation.RESUMEN: Ehrlichia spp. y Rickettsia spp.son dos de los principales géneros rickettsiales transmitidos por garrapatas que afectan a animales silvestres, domésticos y humanos alrededor del mundo. Objetivo: diseñar y validar una prueba PCR duplex para Ehrlichia y Rickettsia en garrapatas. Métodos: la validación de la prueba incluyó ensayos de sensibilidad, especificidad, reproducibilidad y robustez. En la PCR se usó groEL y ARNr 23S como genes blanco para Ehrlichia y Rickettsia, respectivamente. Resultados: el límite de detección fue de 100 copias del gen por 50 μL de reacción para Ehrlichia spp y una copia del gen de Rickettsia por 50 μLde reacción. En general, los cebadores de la prueba solo amplificaron in silico los agentes bacterianos para los cuales fueron originalmente diseñados, con la excepción de los cebadores de Rickettsia que también amplificaron Methylocystis sp. La prueba fue reproducible (precisión intermedia) en un 96.7% de las veces para ambos agentes. La prueba fue suficientemente robusta como para tolerar cambios de concentración de los diferentes reactivos, con excepción de la Taq DNA polimerasa. Conclusión: los resultados de validación indican que la PCR es útil para detectar ambos géneros bacterianos y podría usarse para validación diagnóstica

Amino acids and biogenic amines evolution during the estufagem of fortified wines

The current study was focused on the impact of accelerated ageing (heating step) on the amino acid and biogenic amine profiles of fortified wines. In this sense, three Madeira wines from two commonly used grape varieties (one red and the other white) were analysed during the heating, at standard (45∘ C, 3 months) and overheating (70∘ C, 1 month) conditions, following a precolumn derivatization procedure using iodoacetic acid, o-phthaldialdehyde, and 2-mercaptoethanol, carried out in the injection loop prior to RP-HPLC-FLD detection. Eighteen amino acids were identified, with arginine being the most abundant. An important decrease of the amino acid levels was detected during the standard heating (up to 30%), enhanced up to 61% by the temperature increase. Cysteine, histidine, and asparagine revealed the greatest decreases at 45∘ C. Conversely, some amino acids, such as asparagine, slightly increased. Four biogenic amines were identified but always in trace amounts. Finally, it was observed that the accelerated ageing did not favour the biogenic amine development. The results also indicate that the heating process promotes the amino acid transformation into new ageing products.info:eu-repo/semantics/publishedVersio

Left-right symmetric model with μ↔τ\mu\leftrightarrow\tau symmetry

We analyze the leptonic sector in the left-right symmetric model dressed with a $(Z_{2})^{3}$ discrete symmetry which realizes, after weak spontaneous breaking, a small broken \mu\lra\tau symmetry that is suggested to explain observable neutrino oscillation data. \mu\lra\tau symmetry is broken at tree level in the effective neutrino mass matrix due to the mass difference $\widetilde{m}_{\tau}\neq \widetilde{m}_{\mu}$ in the diagonal Dirac mass terms, whereas all lepton mixings arise from a Majorana mass matrix. In the limit of a small breaking we determined $\theta_{13}$, and the deviation from the maximal value of $\theta_{ATM}$, in terms of the light neutrino hierarchy scale, $m_{3}$, and a single free parameter $h_{s}$ of the model.Comment: 9 pages, 4 figures. Title changed and we have added one new section: CP phase contribution. Minor corrections and references updated. Published versio

Maritime Climate in the Canary Islands and its Implications for the Construction of Coastal Infrastructures

Islands are isolated systems that depend on maritime trade for their subsistence. Efficient, durable and structurally reliable port infrastructures are essential for the economic and social development of islands. However, not all port infrastructures are designed in the same way. They can vary, depending on whether they are built on continental land, built on non-volcanic islands or built on volcanic oceanic islands (such as the Canary Islands, Spain). The latter islands are the subject of this study due to their specific features, construction difficulties and the importance of sound maritime infrastructures. The maritime climate of an area consists of the wave and storm regimes that affect it and, from these, the coastal dynamics and coastal formations of that area can be studied. For this reason, historical data were collated on significant directional wave heights from 1958 to 2015 from several WANA-SIMAR points in the virtual buoy network of State Ports of Spain located near the Canary Islands. These data have been studied to obtain the maximum directional wave heights (Hs) at each point. With this analysis, we have obtained useful summary tables to calculate wave height by a graphic method that transforms the distribution function into a line drawn on probabilistic paper, using reduced variables. This enables adjustments to be made by linear regression and minimum square methods to facilitate planning and design of maritime infrastructures in a reliable way. Doi: 10.28991/CEJ-2022-08-01-02 Full Text: PD

Fruit abundance and trait matching determine diet type and body condition across frugivorous bird populations

Research on seed‐dispersal mutualisms has been highly unbalanced towards the plants, largely overlooking the fitness effects of fruit resources on frugivorous animals. Moreover, despite morphological mismatches like gape limitation may reduce the abundance of fruits that are actually accessible to a frugivore species, there is very little evidence on the trait‐matching implications from a frugivore's perspective. Here, we refine recent resource‐provisioning models to comprehensively test the joint effects of fruit abundance and trait matching on diet type and body condition (a surrogate of fitness) across frugivorous bird populations: Sardinian warblers Curruca melanocephala inhabiting ten Mediterranean forests differing in the abundance and composition of fleshy fruits. We hypothesised the abundance of fruit resources to have positive effects on the degree of frugivory and body condition of warblers, and such effects to be more pronounced when accounting for both trait matching (accessible fruits) and resource provisioning (energy in accessible fruits). We found a sharp threshold over which warblers shifted from a diet with very little or even no fruits to a predominantly frugivorous diet with increasing the local abundance of accessible fruits. We also found a strong positive relationship between the abundance of accessible fruits and the body condition of warblers (body mass and residual body mass), an effect that was more pronounced in females than in males. Although diet type and body condition were much better predicted when accounting for trait matching, accounting for resource provisioning did not improve the explanatory power of fruit resources. The fact that we detected strong and sex‐dependent effects of fruit resources on body condition just a few weeks before the breeding season suggests that fruit resources likely affect the timing and success of reproduction, a question that deserves further research. Our findings provide new insight into the fitness consequences of seed‐dispersal mutualisms for frugivorous animals.info:eu-repo/semantics/acceptedVersio

Paleomagnetic and paleoenvironmental implications of magnetofossil occurrences in late Miocene marine sediments from the Guadalquivir Basin, SW Spain

Although recent studies have revealed more widespread occurrences of magnetofossils in pre-Quaternary sediments than have been previously reported, their significance for paleomagnetic and paleoenvironmental studies is not fully understood. We present a paleo- and rock-magnetic study of late Miocene marine sediments recovered from the Guadalquivir Basin (SW Spain). Well-defined paleomagnetic directions provide a robust magnetostratigraphic chronology for the two studied sediment cores. Rock magnetic results indicate the dominance of intact magnetosome chains throughout the studied sediments. These results provide a link between the highest-quality paleomagnetic directions and higher magnetofossil abundances. We interpret that bacterial magnetite formed in the surface sediment mixed layer and that these magnetic particles gave rise to a paleomagnetic signal in the same way as detrital grains. They, therefore, carry a magnetization that is essentially identical to a post-depositional remanent magnetization, which we term a bio-depositional remanent magnetization. Some studied polarity reversals record paleomagnetic directions with an apparent 60-70 kyr recording delay. Magnetofossils in these cases are interpreted to carry a biogeochemical remanent magnetization that is locked in at greater depth in the sediment column. A sharp decrease in magnetofossil abundance toward the middle of the studied boreholes coincides broadly with a major rise in sediment accumulation rates near the onset of the Messinian salinity crisis (MSC), an event caused by interruption of the connection between the Mediterranean Sea and the Atlantic Ocean. This correlation appears to have resulted from dilution of magnetofossils by enhanced terrigenous inputs that were driven, in turn, by sedimentary changes triggered in the basin at the onset of the MSC. Our results highlight the importance of magnetofossils as carriers of high-quality paleomagnetic and paleoenvironmental signals even in dominantly terrigenous sediments.This study was funded by the Guadaltyc project (MINECO, CGL2012–30875), ARC grant DP120103952, and NSFC grant 41374073

WEASEL: Vodafone Corporate Semantic Web

The 2006 Gartner emerging technology curve highlights the relevance of the Corporate Semantic Web as one of the most promising IT areas in the next five years. The work presented herein describes WEASEL, an initiative funded by Vodafone to apply and evaluate such technology in the context of a large multinational company. This scenario comprises a number of heterogeneous web sites containing unstructured and related, but physically decoupled, information which needs common models that provide unified ways of representing information across the different sources, i.e. ontologies. Three main milestones were defined for WEASEL: the creation of a domain ontology, the extraction of information from the different sources and its semantic annotation and aggregation, and the creation of a new web site containing a semantic search engine which provides natural interfaces for retrieving the aggregated information. WEASEL concluded with its evaluation by Vodafone

Session-based concurrency in Maude:Executable semantics and type checking

Session types are a well-established approach to communication correctness in message-passing processes. Widely studied from a process calculi perspective, here we pursue an unexplored strand and investigate the use of the Maude system for implementing session-typed process languages and reasoning about session-typed process specifications. We present four technical contributions. First, we develop and implement in Maude an executable specification of the operational semantics of a session-typed π-calculus by Vasconcelos. Second, we also develop an executable specification of its associated algorithmic type checking, and describe how both specifications can be integrated. Third, we show that our executable specification can be coupled with reachability and model checking tools in Maude to detect well-typed but deadlocked processes. Finally, we demonstrate the robustness of our approach by adapting it to a higher-order session π-calculus, in which exchanged values include names but also abstractions (functions from names to processes). All in all, our contributions define a promising new approach to the (semi)automated analysis of communication correctness in message-passing concurrency