Aggressiveness of Fusarium oxysporum and F. verticillioides isolates on stone and scots pine under greenhouse conditions

Producción CientíficaScots pine (Pinus sylvestris) and Stone pine (Pinus pinea) are two important species used in re-forestation that are subject to damage by damping-off fungi in forest nurs-eries. Twenty-two isolates of Fusarium oxysporum and F. verticillioides from diseased seedlings of eight different hosts were tested for aggressiveness on seeds and seed-lings of both pine species, including the effects on seedling emergence and mortal-ity. Scots pine was more susceptible to damping-off than Stone pine, as indicated both by reduced seedling emergence and elevated seedling mortality. The impact of F. oxysporum and F. verticillioides on seeds and seedlings did not differ significantly for either pine species. Our findings support previous studies that found that these are damping-off pathogens on the studied pines. Whereas most isolates proved to be pathogenic, some isolates of both Fusarium species showed to be non- pathogenic.Ministerio de Ciencia e Innovación projects (PID2019-110459RB-I00) and (PLEC2021-008076

Changes in the Papez Circuit in early stages of Alzheimer's Disease

Alzheimer's disease (AD) is the most common cause of demenMa. Neuronal and synapMc losses occur iniMally and predominantly in the medial temporal lobe structures including hippocampus, amygdala and thalamus, structures that belong to the Papez circuit. The integrity of the connecMons amongst them is essenMal for episodic memory, which is specifically impaired in AD. For this reason we have invesMgated the degeneraMon paRern of subcorMcal structures and its relaMon to early stages of AD, i.e. Mild CogniMve Impairment (MCI), both in the amnesic and mulMdomain types using structural magneMc resonance imaging (using a 3T GE scanner) and VBM‐DARTEL

Improvement and validation of retrieved FORMOSAT-3/COSMIC electron densities using Jicamarca DPS

Postprint (published version

Experimental supporting data on the influence of platelet-derived factors of malignant pleural effusions on T cell effector functions and their relevance in predicting prognosis of lung adenocarcinoma patients with pleural metastasis

The data described in this article are supplementary to our primary article "Platelet factor 4 regulates T cell effector functions in malignant pleural effusions". Malignant pleural effusion (MPE) is a common complication of advanced lung adenocarcinoma (LAC) associated with a poor life expectancy [1]. Several challenges need to be addressed to identify non-invasive molecular biomarkers that help to predict the prognosis of LAC patients with MPE [2]. In the primary publication, we proposed that platelet-derived factors, especially platelet factor 4 (PF4), can negatively regulate T lymphocyte activation and granzyme B expression in pleural metastasis and its levels were associated with a worse prognosis. Here, we provide data on the influence of other platelet-derived factors, including transforming growth factor β (TGF-β), vascular endothelial factor (VEGF), and P-selectin on T lymphocyte response in MPE and their relevance as prognostic factors in lung cancer patients with pleural metastasis. Pleural fluids from 35 lung adenocarcinoma (LAC) and 20 heart failure (HF) patients were collected by thoracentesis and its platelet-derived factors' content was measured by specific enzyme-linked immunosorbent assay (ELISAs). Correlations between pleural levels of platelet-derived factors and T cell functions were analyzed by Pearson coefficients. Kaplan-Meier curves were used to estimate the effect of pleural concentrations of platelet-derived factors on overall survival of LAC patients with pleural metastasis. These analyses showed that the concentration of platelet-derived factors was not associated with T cell proliferation and cytotoxicity. Furthermore, their levels do not predict the survival of LAC with MPE

Female sex pheromone of the cone moth, dioryctria mendacella: Investigation of synergism between Type I and Type II pheromone components

Polyunsaturated hydrocarbons (Type II pheromone components) have been reported to be synergists for unsaturated acetates, alcohols or aldehydes (Type I components) in the sex pheromones of several species of Lepidoptera. However, there is some debate over whether the active components are the hydrocarbons themselves or more volatile degradation products. Extracts of pheromone glands of adult females of the cone moth, Dioryctria mendacella (Lepidoptera: Pyralidae), contain (Z,E)-9,11-tetradecadienyl acetate (ZE9,11-14:Ac) and at least ten times as much (Z,Z,Z,Z,Z)-3,6,9,12,15-pentacosapentaene (ZZZZZ3,6,9,12,15-25:H). The former elicits a strong electroantennogram response from males while no response could be recorded to the latter. In field trapping tests, both compounds were individually unattractive to male D. mendacella moths, but blends of the two compounds containing at least a 10:1 ratio of ZZZZZ3,6,9,12,15-25:H : ZE9,11-14:Ac were highly attractive. The relatively involatile hydrocarbon was shown to be released from the dispensers used and no significant degradation could be detected. Furthermore, blends of ZE9,11-14:Ac and analogs of ZZZZZ3,6,9,12,15-25:H with fewer carbons and/or double bonds that might be expected to produce similar degradation products to ZZZZZ3,6,9,12,15-25:H were unattractive. This indicated a specific response to the hydrocarbon itself, further substantiated by the observation that related hydrocarbons did not interfere with the activity of ZZZZZ3,6,9,12,15-25:H. Thus a three-step conversion of fish oil was used to produce a blend of unsaturated hydrocarbons containing ZZZZZ3,6,9,12,15-25:H as the major component, albeit only 30% of the total, and a blend of this material with ZE9,11-14:Ac was as attractive to male D. mendacella moths as blends with an equivalent amount of the purified material. This mixture of unsaturated hydrocarbons is much cheaper to produce than the pure pentaene, and may be useful in lures for other species using these compounds. Dioryctria mendacella is a major constraint to production of edible pine kernels throughout the Mediterranean region. Pheromone traps will provide a means to improve monitoring of seasonal flight patterns and changes in population abundance of this pest

Relaciones filogenéticas en Erysimun (Brassicaceae) de las Cordilleras Béticas (SE Península Ibérica)

The Baetic mountains, located in the southern Iberian Peninsula, is a major hotspot of biodiversity in the Mediterranean Basin, constituting one of the most important glacial refugia for vascular plants in Europe. Despite their relatively limited extension, the Baetic Mountains contain almost 50% of the total endemic Erysimum species in the Iberian Peninsula. The broadly distributed Erysimum genus has diversified profusely in the Mediterranean region, with more than a hundred species described in the area, out of a total of c. 200 species included in the genus. We used two plastid DNA regions (ndhF and trnT-L) and one nuclear DNA region (ITS1-5.8S rDNA-ITS2), with 3,556 bp total length, to carry out phylogenetic analysis by Bayesian inference, maximum likelihood and maximum parsimony, in order to explore the evolutionary relationships between the Erysimum species inhabiting these ranges. Analyses of concatenated sequences from the two genomes identified two main clades with no overlap in species composition so that samples from the same species fell within the same major clade. The phylogenetic relationships depicted by those two clades do not give support to the E. nevadense group, previously proposed on taxonomic grounds. In addition, our results indicated recurrent changes in flower colour in the Baetic Erysimum species although, alternatively, reticulate evolution, which is suggested by incongruent position of taxa in the different trees, may have also affected this trait.Las cordilleras Béticas, localizadas en el sudeste de la Península Ibérica, representan una importante zona para la biodiversidad de la cuenca mediterránea, constituyendo uno de los refugios glaciares más destacados de plantas vasculares en Europa. A pesar de su extensión relativamente limitada, las cordilleras Béticas albergan casi el 50% del total de las especies endémicas de Erysimum de la Península Ibérica. Erysimum es un género ampliamente distribuido, que se ha diversificado profusamente en la región mediterránea, con más de un centenar de especies descritas en dicha área, del total de las alrededor de 200 especies incluidas en el género. Usamos dos regiones de ADN plastidial (ndhF y trnT-L) y una región de ADN nuclear (ITS1-5.8S rDNA-ITS2), con una longitud total de 3.556 pb, para llevar a cabo análisis filogenéticos mediante inferencia bayesiana, máxima verosimilitud y máxima parsimonia, con el fin de explorar las relaciones evolutivas entre las especies de Erysimum que habitan en éstas cordilleras. El análisis de secuencias concatenadas de los dos genomas identifica dos clados principales y las muestras de la misma especie aparecen siempre en un clado o en el otro pero no en ambos. Las relaciones filogenéticas que indican esos dos clados no apoyan la circunscripción del grupo taxonómico E. nevadense, previamente definido en dicho género. Además, los análisis indican cambios recurrentes en el color floral de las especies de Erysimum que habitan los Sistemas Béticos aunque la evolución reticulada, que sugiere la posición de algunos taxones en uno y otro árbol, puede haber también afectado a este rasgo, lo que sería una hipótesis alternativa.This study has been supported by MARM (078/2007), Junta de Andalucía (P07-RNM-02869 and P11-RNM-7676), MONTES Consolider-Ingenio (CSD2008-00040), and Spanish MCyT (CGL2009-07015, and CGL2012-34736) grants. AJMP and MA were supported by Spanish Education and Science grants AP-2006- 00685 and BES-2007-14206, respectively

Reporte de falla terapéutica de Fenbendazol, Levamisol e Ivermectina en el control de nematodos gastrointestinales en ganado lechero (Bos taurus) en Cajamarca, Perú

The present study evaluated the efficacy of fenbendazole, levamisole and ivermectin in the control of nematodes in dairy cattle farms in three provinces of the Cajamarca region, Peru. Thirty female cattle older than six months were selected in each farm (E-I, E-II, E-III), distributed in three homogeneous groups (n=10) according to the natural infection loads of gastrointestinal nematodes. Using an active principle for each treatment, fenbendazole (oral) and levamisole (subcutaneous) were administered at a dose of 7.5 mg/kg and ivermectin at 0.2 mg/kg (subcutaneous). It was determined that in E-I there was therapeutic failure to fenbendazole (70.05 ± 6.57% efficacy) and levamisole (52.08 ± 7.07%) and in E-II to levamisole (65.06 ± 7.25%) and ivermectin (73.96 ± 6.62%). In E-III, levamisole and fenbendazole were effective (99.13 ± 0.98% and 100%, respectively). The larvae obtained after dosing were Trichostrongylus spp and Ostertagia spp in E-I, Haemonchus spp and Ostertagia spp in E-II, and Trichostrongylus spp and Ostertagia spp in E-III.El presente estudio evalúa la eficacia de fenbendazol, levamisol e ivermectina en el control de nematodos en bovinos lecheros de predios de tres provincias de la región Cajamarca, Perú. Se utilizaron 30 hembras bovinas mayores de seis meses por cada predio (E-I, E-II y E-III), distribuidas en tres grupos homogéneos (n=10) de acuerdo con las cargas de infección natural de nematodos gastrointestinales. Utilizando un principio activo para cada tratamiento, se administró fenbendazol (oral) y levamisol (subcutáneo) a dosis de 7.5 mg/kg e ivermectina a 0.2 mg/kg (subcutáneo). Se determinó que en el E-I hubo falla terapéutica a fenbendazol (70.05 ± 6.57% de eficacia) y a levamisol (52.08 ± 7.07%) y en E-II a levamisol (65.06 ± 7.25%) e ivermectina (73.96 ± 6.62%). En E-III fueron eficaces levamisol y fenbendazol (99.13 ± 0.98% y 100%, respectivamente).. Las larvas obtenidas después de la dosificación fueron Trichostrongylus spp y Ostertagia spp en E-I, Haemonchus spp y Ostertagia spp en E-II, y Trichostrongylus spp y Ostertagia spp en E-III

Ground- and space-based GPS data ingestion into the NeQuick model

This paper presents a technique for ingesting ground- and space-based dual-frequency GPS observations into a semi-empirical global electron density model. The NeQuick-2 model is used as the basis for describing the global electron density distribution. This model is mainly driven by the F2 ionosphere layer parameters (i.e. the electron density, NmF2, and the height, hmF2 of the F2 peak), which, in the absence of directly measured values, are computed from the ITU-R database (ITU-R 1997). This database was established using observations collected from 1954 to 1958 by a network of around 150 ionospheric sounders with uneven global coverage. It allows computing monthly median values of NmF2 and hmF2 (intra-month variations are averaged), for low and high solar activity. For intermediate solar activity a linear interpolation must be performed. Ground-based GNSS observations from a global network of ~350 receivers are pre-processed in order to retrieve slant total electron content (sTEC) information, and space-based GPS observations (radio occultation data from the FORMOSAT-3/COSMIC constellation) are pre-processed to retrieve electron density (ED) information. Both, sTEC and ED are ingested into the NeQuick-2 model in order to adapt NmF2 and hmF2, and reduce simultaneously both, the observed minus computed sTEC and ED differences. The first experimental results presented in this paper suggest that the data ingestion technique is self consistent and able to reduce the observed minus computed sTEC and ED differences to ~25–30% of the values computed from the ITU-R database. Although sTEC and ED are both derived from GPS observations, independent algorithm and models are used to compute their values from ground-based GPS observations and space-based FORMOSAT-3/COSMIC radio occultations. This fact encourages us to pursue this research with the aim to improve the results presented here and assess their accuracy in a reliable way.Facultad de Ciencias Astronómicas y Geofísica

Identification of novel synthetic lethal vulnerability in non small cell lung cancer by co targeting TMPRSS4 and DDR1

Finding novel targets in non-small cell lung cancer (NSCLC) is highly needed and identification of synthetic lethality between two genes is a new approach to target NSCLC. We previously found that TMPRSS4 promotes NSCLC growth and constitutes a prognostic biomarker. Here, through large-scale analyses across 5 public databases we identified consistent co-expression between TMPRSS4 and DDR1. Similar to TMPRSS4, DDR1 promoter was hypomethylated in NSCLC in 3 independent cohorts and hypomethylation was an independent prognostic factor of disease-free survival. Treatment with 5-azacitidine increased DDR1 levels in cell lines, suggesting an epigenetic regulation. Cells lacking TMPRSS4 were highly sensitive to the cytotoxic effect of the DDR1 inhibitor dasatinib. TMPRSS4/DDR1 double knock-down (KD) cells, but not single KD cells suffered a G0/G1 cell cycle arrest with loss of E2F1 and cyclins A and B, increased p21 levels and a larger number of cells in apoptosis. Moreover, double KD cells were highly sensitized to cisplatin, which caused massive apoptosis (~40%). In vivo studies demonstrated tumor regression in double KD-injected mice. In conclusion, we have identified a novel vulnerability in NSCLC resulting from a synthetic lethal interaction between DDR1 and TMPRSS4