Cleaning up nitrogen pollution may reduce future carbon sinks

Biosphere carbon sinks are crucial for reducing atmospheric carbon dioxide (CO2) concentration to mitigate global warming, but are substantially affected by the input of reactive nitrogen (Nr). Although the effects of anthropogenic CO2 emission and nitrogen deposition (indicated by Nr emission to atmosphere) on carbon sink have been studied, it is unclear how their ratio (C/N) changes with economic development and how such change alters biosphere carbon sinks. Here, by compiling datasets for 132 countries we find that the C/N ratio continued to increase despite anthropogenic CO2 and Nr emissions to atmosphere both showing an asymmetric para-curve with economic growth. The inflection points of CO2 and Nr emissions are found at around $15,000 gross domestic product per capita worldwide. Economic growth promotes the use of Nr and energy, while at the same time increases their use efficiencies, together resulting in occurrences of inflection points of CO2 and Nr emissions. Nr emissions increase slower but decrease faster than that of CO2 emissions before and after the inflection point, respectively. It implies that there will be relatively more anthropogenic CO2 emission but less N deposition with economic growth. This may limit biosphere carbon sink because of relative shortage of Nr. This finding should be integrated/included in global climate change modelling. Efforts, such as matching N deposition with carbon sequestration on regional scale, to manage CO2 and Nr emissions comprehensively to maintain a balance are critical

Robust estimation of bacterial cell count from optical density

Optical density (OD) is widely used to estimate the density of cells in liquid culture, but cannot be compared between instruments without a standardized calibration protocol and is challenging to relate to actual cell count. We address this with an interlaboratory study comparing three simple, low-cost, and highly accessible OD calibration protocols across 244 laboratories, applied to eight strains of constitutive GFP-expressing E. coli. Based on our results, we recommend calibrating OD to estimated cell count using serial dilution of silica microspheres, which produces highly precise calibration (95.5% of residuals <1.2-fold), is easily assessed for quality control, also assesses instrument effective linear range, and can be combined with fluorescence calibration to obtain units of Molecules of Equivalent Fluorescein (MEFL) per cell, allowing direct comparison and data fusion with flow cytometry measurements: in our study, fluorescence per cell measurements showed only a 1.07-fold mean difference between plate reader and flow cytometry data

High-coverage whole-genome analysis of 1220 cancers reveals hundreds of genes deregulated by rearrangement-mediated cis-regulatory alterations.

The impact of somatic structural variants (SVs) on gene expression in cancer is largely unknown. Here, as part of the ICGC/TCGA Pan-Cancer Analysis of Whole Genomes (PCAWG) Consortium, which aggregated whole-genome sequencing data and RNA sequencing from a common set of 1220 cancer cases, we report hundreds of genes for which the presence within 100 kb of an SV breakpoint associates with altered expression. For the majority of these genes, expression increases rather than decreases with corresponding breakpoint events. Up-regulated cancer-associated genes impacted by this phenomenon include TERT, MDM2, CDK4, ERBB2, CD274, PDCD1LG2, and IGF2. TERT-associated breakpoints involve ~3% of cases, most frequently in liver biliary, melanoma, sarcoma, stomach, and kidney cancers. SVs associated with up-regulation of PD1 and PDL1 genes involve ~1% of non-amplified cases. For many genes, SVs are significantly associated with increased numbers or greater proximity of enhancer regulatory elements near the gene. DNA methylation near the promoter is often increased with nearby SV breakpoint, which may involve inactivation of repressor elements

Retrospective evaluation of whole exome and genome mutation calls in 746 cancer samples

Funder: NCI U24CA211006Abstract: The Cancer Genome Atlas (TCGA) and International Cancer Genome Consortium (ICGC) curated consensus somatic mutation calls using whole exome sequencing (WES) and whole genome sequencing (WGS), respectively. Here, as part of the ICGC/TCGA Pan-Cancer Analysis of Whole Genomes (PCAWG) Consortium, which aggregated whole genome sequencing data from 2,658 cancers across 38 tumour types, we compare WES and WGS side-by-side from 746 TCGA samples, finding that ~80% of mutations overlap in covered exonic regions. We estimate that low variant allele fraction (VAF < 15%) and clonal heterogeneity contribute up to 68% of private WGS mutations and 71% of private WES mutations. We observe that ~30% of private WGS mutations trace to mutations identified by a single variant caller in WES consensus efforts. WGS captures both ~50% more variation in exonic regions and un-observed mutations in loci with variable GC-content. Together, our analysis highlights technological divergences between two reproducible somatic variant detection efforts

Quantitative Relationships between the Tidal Current Limit, Tidal Level Limit and River Discharge in the Changjiang River Estuary

Estuaries are areas where runoff and tide interact. Tidal waves propagate upstream from river mouths and produce tidal currents and tidal level variations along rivers. Based on the hydrological frequency analysis of river discharge in the dry season and flood season at the Datong hydrological station over the past 70 years, a three-dimensional estuary numerical model was used to produce the quantitative relationships between the tidal current limit, tidal level limit and river discharge in the Changjiang River estuary. The positions of tidal current limit and tidal level limit depend not only on river discharge but also on river topography. When river discharge varies from a hydrological frequency of 95% to 5%, the relationship between the tidal current limit and river discharge is y=2×10−13x3+3&nbsp;×&nbsp;10−8x2−&nbsp;0.0074x+359.35 in the flood season, with a variation range of 90 km, and y=−4×10−10x3−1&nbsp;×&nbsp;10−5x2−0.1937x&nbsp;−&nbsp;1232.9 in the dry season, with a variation range of 200 km. The relationship between the tidal level limit and river discharge is y=6×10−8x2−0.0096x+775.94 in the flood season, with a variation range of 127 km, and y=0.3428x2−17.9x+777.55 in the dry season, with a variation range of 83 km, which is located far upstream of the Datong hydrological station

Research on Medical Security System Based on Zero Trust

With the rapid development of Internet of Things technology, cloud computing, and big data, the combination of medical systems and information technology has become increasingly close. However, the emergence of intelligent medical systems has brought a series of network security threats and hidden dangers, including data leakage and remote attacks, which can directly threaten patients’ lives. To ensure the security of medical information systems and expand the application of zero trust in the medical field, we combined the medical system with the zero-trust security system to propose a zero-trust medical security system. In addition, in its dynamic access control module, based on the RBAC model and the calculation of user behavior risk value and trust, an access control model based on subject behavior evaluation under zero-trust conditions (ABEAC) was designed to improve the security of medical equipment and data. Finally, the feasibility of the system is verified through a simulation experiment

Review on Parameterization Schemes of Visibility in Fog and Brief Discussion of Applications Performance

Low visibility, associated with fog, severely affects land, marine, and air transportation. Visibility is an important indicator to identify different intensities of fog; therefore, improving the ability to forecast visibility in fog is an urgent need for social and economic development. Establishing a proper visibility parameterization scheme is crucial to improving the accuracy of fog forecast operation. Considering various visibility impact factors, including RH, Nd, D, LWC, the parameterization formula of visibility in fog, as well as their performance in meteorology operation, are reviewed. Moreover, the estimated ability of the visibility parameterization formulas combined with the numerical model is briefly described, and their advantages and shortcomings are pointed out

Insight into the Inhibitory Mechanism of Aryl Formyl Piperidine Derivatives on Monoacylglycerol Lipase through Molecular Dynamics Simulations

Monoacylglycerol lipase (MAGL) can regulate the endocannabinoid system and thus becomes a target of antidepressant drugs. In this paper, molecular docking and molecular dynamics simulations, combined with binding free energy calculation, were employed to investigate the inhibitory mechanism and binding modes of four aryl formyl piperidine derivative inhibitors with different 1-substituents to MAGL. The results showed that in the four systems, the main four regions where the enzyme bound to the inhibitor included around the head aromatic ring, the head carbonyl oxygen, the tail amide bond, and the tail benzene ring. The significant conformational changes in the more flexible lid domain of the enzyme were caused by 1-substituted group differences of inhibitors and resulted in different degrees of flipping in the tail of the inhibitor. The flipping led to a different direction of the tail amide bond and made a greater variation in its interaction with some of the charged residues in the enzyme, which further contributed to a different swing of the tail benzene ring. If the swing is large enough, it can weaken the binding strength of the head carbonyl oxygen to its nearby residues, and even the whole inhibitor with the enzyme so that the inhibition decreases

Bifunctional MXene‐Augmented Retinal Progenitor Cell Transplantation for Retinal Degeneration

Abstract Retinal degeneration, characterized by the progressive loss of retinal neurons, is the leading cause of incurable visual impairment. Retinal progenitor cells (RPCs)‐based transplantation can facilitate sight restoration, but the clinical efficacy of this process is compromised by the imprecise neurogenic differentiation of RPCs and undermining function of transplanted cells surrounded by severely oxidative retinal lesions. Here, it is shown that ultrathin niobium carbide (Nb2C) MXene enables performance enhancement of RPCs for retinal regeneration. Nb2C MXene with moderate photothermal effect markedly improves retinal neuronal differentiation of RPCs by activating intracellular signaling, in addition to the highly effective RPC protection by scavenging free radicals concurrently, which has been solidly evidenced by the comprehensive biomedical assessments and theoretical calculations. A dramatically increased neuronal differentiation is observed upon subretinal transplantation of MXene‐assisted RPCs into the typical retinal degeneration 10 (rd10) mice, thereby contributing to the efficient restoration of retinal architecture and visual function. The dual‐intrinsic function of MXene synergistically aids RPC transplantation, which represents an intriguing paradigm in vision‐restoration research filed, and will broaden the multifunctionality horizon of nanomedicine