150 research outputs found
Deformations of calibrated D-branes in flux generalized complex manifolds
We study massless deformations of generalized calibrated cycles, which
describe, in the language of generalized complex geometry, supersymmetric
D-branes in N=1 supersymmetric compactifications with fluxes. We find that the
deformations are classified by the first cohomology group of a Lie algebroid
canonically associated to the generalized calibrated cycle, seen as a
generalized complex submanifold with respect to the integrable generalized
complex structure of the bulk. We provide examples in the SU(3) structure case
and in a `genuine' generalized complex structure case. We discuss cases of
lifting of massless modes due to world-volume fluxes, background fluxes and a
generalized complex structure that changes type.Comment: 52 pages, added references, added comment on ellipticity in appendix
B, made minor changes according to instructions referee JHE
Direct and indirect control of the initiation of meiotic recombination by DNA damage checkpoint mechanisms in budding yeast
Meiotic recombination plays an essential role in the proper segregation of chromosomes at meiosis I in many sexually reproducing organisms. Meiotic recombination is initiated by the scheduled formation of genome-wide DNA double-strand breaks (DSBs). The timing of DSB formation is strictly controlled because unscheduled DSB formation is detrimental to genome integrity. Here, we investigated the role of DNA damage checkpoint mechanisms in the control of meiotic DSB formation using budding yeast. By using recombination defective mutants in which meiotic DSBs are not repaired, the effect of DNA damage checkpoint mutations on DSB formation was evaluated. The Tel1 (ATM) pathway mainly responds to unresected DSB ends, thus the sae2 mutant background in which DSB ends remain intact was employed. On the other hand, the Mec1 (ATR) pathway is primarily used when DSB ends are resected, thus the rad51 dmc1 double mutant background was employed in which highly resected DSBs accumulate. In order to separate the effect caused by unscheduled cell cycle progression, which is often associated with DNA damage checkpoint defects, we also employed the ndt80 mutation which permanently arrests the meiotic cell cycle at prophase I. In the absence of Tel1, DSB formation was reduced in larger chromosomes (IV, VII, II and XI) whereas no significant reduction was found in smaller chromosomes (III and VI). On the other hand, the absence of Rad17 (a critical component of the ATR pathway) lead to an increase in DSB formation (chromosomes VII and II were tested). We propose that, within prophase I, the Tel1 pathway facilitates DSB formation, especially in bigger chromosomes, while the Mec1 pathway negatively regulates DSB formation. We also identified prophase I exit, which is under the control of the DNA damage checkpoint machinery, to be a critical event associated with down-regulating meiotic DSB formation
Measurement of the solar 8B neutrino rate with a liquid scintillator target and 3 MeV energy threshold in the Borexino detector
We report the measurement of electron neutrino elastic scattering from 8B
solar neutrinos with 3 MeV energy threshold by the Borexino detector in Gran
Sasso (Italy). The rate of solar neutrino-induced electron scattering events
above this energy in Borexino is 0.217 +- 0.038 (stat) +- 0.008 (syst) cpd/100
t, which corresponds to the equivalent unoscillated flux of (2.4 +- 0.4 (stat)
+- 0.1 (syst))x10^6 cm^-2 s^-1, in good agreement with measurements from SNO
and SuperKamiokaNDE. Assuming the 8B neutrino flux predicted by the high
metallicity Standard Solar Model, the average 8B neutrino survival probability
above 3 MeV is measured to be 0.29+-0.10. The survival probabilities for 7Be
and 8B neutrinos as measured by Borexino differ by 1.9 sigma. These results are
consistent with the prediction of the MSW-LMA solution of a transition in the
solar electron neutrino survival probability between the low energy
vacuum-driven and the high-energy matter-enhanced solar neutrino oscillation
regimes.Comment: 10 pages, 8 figures, 6 table
Inhibition of activation induced CD154 on CD4+ CD25- cells: a valid surrogate for human Treg suppressor function
Natural Regulatory T cells (Tregs) are defined by stable expression of the cell surface proteins CD4 and CD25, low surface expression of CD127 and expression of the transcription factor FOXP3. The contribution of Treg to the prevention of autoimmunity and the maintenance of immune homoestasis is the subject of ongoing interest, as alterations in Treg numbers and function are implicated in a wide range of diseases. The in vitro benchmark for determining Treg function is suppression of proliferation of unmatched effector T cells in a mixed lymphocyte reaction (MLR) over a 3–6-day time period. As an alternative to this assay, we show that a 7-h CD154 expression assay is rapid, simple and provides a reliable readout of suppressor function. Using multiple Treg-like cell types including natural (n)Treg, inducible (i)Treg and Treg cell lines, we show that suppression of CD154 expression is a surrogate for suppression of proliferation. We propose this as a suitable alternative to the MLR assay, as it is rapid and may be more amenable to high-throughput screening, analysing large cohorts of clinical samples or assaying transiently suppressive populations. Keywords: regulatory T cells; functional assays; iTreg; nTregDanika Hill, Nicola Eastaff-Leung, Suzanne Bresatz-Atkins, Noel Warner, Joyce Ruitenberg, Dorren Krumbiegel, Steve Pederson, Natasha McInnes, Cheryl Y. Brown, Timothy Sadlon and Simon C. Barr
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New experimental limits on the Pauli forbidden transitions in C nuclei obtained with 485 days Borexino data
The Pauli exclusion principle (PEP) has been tested for nucleons () in
with the Borexino detector.The approach consists of a search for
, , and emitted in a non-Paulian transition of
1- shell nucleons to the filled 1 shell in nuclei. Due to the
extremely low background and the large mass (278 t) of the Borexino detector,
the following most stringent up-to-date experimental bounds on PEP violating
transitions of nucleons have been established:
y, y,
y,
y and y, all at 90% C.L. The corresponding upper
limits on the relative strengths for the searched non-Paulian electromagnetic,
strong and weak transitions have been estimated: , and .Comment: 9 pages, 6 figure
Recommended from our members
Observation of Geo-Neutrinos
Geo-neutrinos, electron anti-neutrinos produced in beta decays of naturally
occurring radioactive isotopes in the Earth, are a unique direct probe of our
planet's interior. We report the first observation at more than 3 C.L.
of geo-neutrinos, performed with the Borexino detector at Laboratori Nazionali
del Gran Sasso. Anti-neutrinos are detected through the neutron inverse beta
decay reaction. With a 252.6 ton-yr fiducial exposure after all selection cuts,
we detected 9.9^{+4.1}_{-3.4}(^{+14.6}_{-8.2}) geo-neutrino events, with errors
corresponding to a 68.3%(99.73%) C.L. From the profile, the
statistical significance of the Borexino geo-neutrino observation corresponds
to a 99.997% C.L. Our measurement of the geo-neutrinos rate is
3.9^{+1.6}_{-1.3}(^{+5.8}_{-3.2}) events/(100ton-yr). This measurement rejects
the hypothesis of an active geo-reactor in the Earth's core with a power above
3 TW at 95% C.L. The observed prompt positron spectrum above 2.6 MeV is
compatible with that expected from european nuclear reactors (mean base line of
approximately 1000 km). Our measurement of reactor anti-neutrinos excludes the
non-oscillation hypothesis at 99.60% C.L.Comment: 8 pages, 4 figures, 3 table
Neutrino Propagation in a Strongly Magnetized Medium
We derive general expressions at the one-loop level for the coefficients of
the covariant structure of the neutrino self-energy in the presence of a
constant magnetic field. The neutrino energy spectrum and index of refraction
are obtained for neutral and charged media in the strong-field limit () using the lowest Landau level
approximation. The results found within the lowest Landau level approximation
are numerically validated, summing in all Landau levels, for strong and weakly-strong fields. The neutrino energy in
leading order of the Fermi coupling constant is expressed as the sum of three
terms: a kinetic-energy term, a term of interaction between the magnetic field
and an induced neutrino magnetic moment, and a rest-energy term. The leading
radiative correction to the kinetic-energy term depends linearly on the
magnetic field strength and is independent of the chemical potential. The other
two terms are only present in a charged medium. For strong and weakly-strong
fields, it is found that the field-dependent correction to the neutrino energy
in a neutral medium is much larger than the thermal one. Possible applications
to cosmology and astrophysics are considered.Comment: 23 pages, 4 figures. Corrected misprints in reference
New results on solar neutrino fluxes from 192 days of Borexino data
We report the direct measurement of the ^7Be solar neutrino signal rate
performed with the Borexino detector at the Laboratori Nazionali del Gran
Sasso. The interaction rate of the 0.862 MeV ^7Be neutrinos is
49+-3(stat)+-4(syst) counts/(day * 100ton). The hypothesis of no oscillation
for ^7Be solar neutrinos is inconsistent with our measurement at the 4sigma
level. Our result is the first direct measurement of the survival probability
for solar nu_e in the transition region between matter-enhanced and
vacuum-driven oscillations. The measurement improves the experimental
determination of the flux of ^7Be, pp, and CNO solar nu_e, and the limit on the
magnetic moment of neutrinos
The Borexino detector at the Laboratori Nazionali del Gran Sasso
Borexino, a large volume detector for low energy neutrino spectroscopy, is
currently running underground at the Laboratori Nazionali del Gran Sasso,
Italy. The main goal of the experiment is the real-time measurement of sub MeV
solar neutrinos, and particularly of the mono energetic (862 keV) Be7 electron
capture neutrinos, via neutrino-electron scattering in an ultra-pure liquid
scintillator. This paper is mostly devoted to the description of the detector
structure, the photomultipliers, the electronics, and the trigger and
calibration systems. The real performance of the detector, which always meets,
and sometimes exceeds, design expectations, is also shown. Some important
aspects of the Borexino project, i.e. the fluid handling plants, the
purification techniques and the filling procedures, are not covered in this
paper and are, or will be, published elsewhere (see Introduction and
Bibliography).Comment: 37 pages, 43 figures, to be submitted to NI
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