Analyses spectroscopiques du liquide céphalo-rachidien de rat en ex vivo et du noyau du raphé dorsal in vivo

Les propriétés d'absorption et de fluorescence du liquide céphalo-rachidien (LCR) ponctionné au niveau de la cisterna magna du rat, sont analysées puis comparées à l'émission mesurée in situ dans le noyau du raphe dorsal du rat libre de tous mouvements. Les mesures de fluorescence en ex vivo du LCR et in vivo du noyau raphé dorsal, ont été réalisées par la mise en œuvre d'un microcapteur à fibre optique (FOCS). La fluorescence mesurée in vivo sous excitation à 337 nm, présente 2 pics d'émission situés vers 410 et 460 nm. Les spectres d'absorption, d'émission en fluorescence statique et en fluorescence induite par laser sont rapportés. Avec des domaines de longueur d'onde d'excitation de 300-315 nm, 320-355 nm et 360-470 nm, les spectres d'émission du LCR en ex vivo montrent respectivement des pics centrés vers 340 nm, 390 nm et 530 nm. Malgré les limites liées aux différences de localisation anatomique, ces approches ainsi que celles de la littérature permettent de suggérer que le signal de fluorescence mesuré in vivo à 460nm pourrait dépendre pour une grande partie du NADH intracellulaire

Observational constraints on the sensitivity of two calving glaciers to external forcings

Future mass loss projections of the Greenland ice sheet require understanding of the processes at a glacier terminus, especially of iceberg calving. We present detailed and high-rate terrestrial radar interferometer observations of Eqip Sermia and Bowdoin Glacier, two outlet glaciers in Greenland with comparable dimensions and investigate iceberg calving, surface elevation, velocity, strain rates and their links to air temperature, tides and topography. The results reveal that the two glaciers exhibit very different flow and calving behaviour on different timescales. Ice flow driven by a steep surface slope with several topographic steps leads to high velocities, areas of extension and intense crevassing, which triggers frequent but small calving events independent of local velocity gradients. In contrast, ice flow under smooth surface slopes leaves the ice relatively intact, such that sporadic large-scale calving events dominate, which initiate in areas with high shearing. Flow acceleration caused by enhanced meltwater input and tidal velocity variations were observed for terminus sections close to floatation. Firmly grounded terminus sections showed no tidal signal and a weak short-term reaction to air temperature. These results demonstrate reaction timescales to external forcings from hours to months, which are, however, strongly dependent on local terminus geometry

One-Parameter GHG Emission Policy with R&D-based Growth

This document examines the GHG emission policy of regions which use land, labor and emitting inputs in production and enhance their productivity by devoting labor to R&D, but with different endowments and technology. The regions also have different impacts on global pollution. The problem is to organize common emission policy, if the regions cannot form a federation with a common budget and the policy parameters must be uniform for all regions. The results are the following. If a self-interested central planner allocate emission caps in fixed proportion to past emissions (i.e. grandfathering), then it establishes the Pareto optimum, decreasing emissions and promoting R&D and economic growth

The implausibility of ‘usual care’ in an open system: sedation and weaning practices in Paediatric Intensive Care Units (PICUs) in the United Kingdom (UK)

Background: The power of the randomised controlled trial depends upon its capacity to operate in a closed system whereby the intervention is the only causal force acting upon the experimental group and absent in the control group, permitting a valid assessment of intervention efficacy. Conversely, clinical arenas are open systems where factors relating to context, resources, interpretation and actions of individuals will affect implementation and effectiveness of interventions. Consequently, the comparator (usual care) can be difficult to define and variable in multi-centre trials. Hence outcomes cannot be understood without considering usual care and factors that may affect implementation and impact on the intervention. Methods: Using a fieldwork approach, we describe PICU context, ‘usual’ practice in sedation and weaning from mechanical ventilation, and factors affecting implementation prior to designing a trial involving a sedation and ventilation weaning intervention. We collected data from 23 UK PICUs between June and November 2014 using observation, individual and multi-disciplinary group interviews with staff. Results: Pain and sedation practices were broadly similar in terms of drug usage and assessment tools. Sedation protocols linking assessment to appropriate titration of sedatives and sedation holds were rarely used (9 % and 4 % of PICUs respectively). Ventilator weaning was primarily a medical-led process with 39 % of PICUs engaging senior nurses in the process: weaning protocols were rarely used (9 % of PICUs). Weaning methods were variably based on clinician preference. No formal criteria or use of spontaneous breathing trials were used to test weaning readiness. Seventeen PICUs (74 %) had prior engagement in multi-centre trials, but limited research nurse availability. Barriers to previous trial implementation were intervention complexity, lack of belief in the evidence and inadequate training. Facilitating factors were senior staff buy-in and dedicated research nurse provision. Conclusions: We examined and identified contextual and organisational factors that may impact on the implementation of our intervention. We found usual practice relating to sedation, analgesia and ventilator weaning broadly similar, yet distinctively different from our proposed intervention, providing assurance in our ability to evaluate intervention effects. The data will enable us to develop an implementation plan; considering these factors we can more fully understand their impact on study outcomes

Modeling the re-appearance of a crashed airplane on Gauligletscher, Switzerland

In this study we used a modeling approach to reconstruct the space-time trajectory of the Dakota airplane which crashed on the Gauligletscher in 1946 and was subsequently buried by snow accumulation. Our aim was to localize its present position and predict when and where it would re-appear at the surface. As a first step we modeled the ice flow field and the evolution of Gauligletscher from 1946 using a combined Stokes ice flow and surface mass balance model, which was calibrated with surface elevation and velocity observations. In a second step the modeled ice velocity fields were integrated forward-in-time, starting from the crash location. Our results suggest that the main body of the damaged aircraft will be released approximately between 2027 and 2035, 1 km upstream of the parts that emerged between 2012 and 2018. Our modeling results indicate that the recently found pieces of the Dakota might have been removed from the original aircraft location and moved down-glacier before being abandoned in the late 40s

Forming Terrains by Glacial Erosion

We introduce the first solution for simulating the formation and evolution of glaciers, together with their attendant erosive effects, for periods covering the combination of glacial and inter-glacial cycles. Our efficient solution includes both a fast yet accurate deep learning-based estimation of high- order ice flows and a new, multi-scale advection scheme enabling us to account for the distinct time scales at which glaciers reach equilibrium compared to eroding the terrain. We combine the resulting glacial erosion model with finer-scale erosive phenomena to account for the transport of debris flowing from cliffs. This enables us to model the formation of terrain shapes not previously adequately modeled in Computer Graphics, ranging from U-shaped and hanging valleys to fjords and glacial lakes

Excited States of Proton-bound DNA/RNA Base Homo-dimers: Pyrimidines

We are presenting the electronic photo fragment spectra of the protonated pyrimidine DNA bases homo-dimers. Only the thymine dimer exhibits a well structured vibrational progression, while protonated monomer shows broad vibrational bands. This shows that proton bonding can block some non radiative processes present in the monomer.Comment: We acknowledge the use of the computing facility cluster GMPCS of the LUMAT federation (FR LUMAT 2764