Impact of laser-assisted hatching on pregnancy rate in fresh and frozen-thawed cycles

peer reviewe

Recent trends in multi-block data analysis in chemometrics for multi-source data integration

In recent years, multi-modal measurements of process and product properties have become widely popular. Sometimes classical chemometric methods such as principal component analysis (PCA) and partial least squares regression (PLS) are not adequate to analyze this kind of data. In recent years, several multi-block methods have emerged for this purpose; however, their use is largely limited to chemometricians, and non-experts have little experience with such methods. In order to deal with this, the present review provides a brief overview of the multi-block data analysis concept, the various tasks that can be performed with it and the advantages and disadvantages of different techniques. Moreover, basic tasks ranging from multi-block data visualization to advanced innovative applications such as calibration transfer will be briefly highlighted. Finally, a summary of software resources available for multi-block data analysis is provided

Anaphylactic Shock A Form of Distributive Shock without Inhibition of Oxygen Consumption

Background: The pathophysiology of anaphylactic shock during anesthesia is incompletely characterized. It is described as distributive by analogy with septic shock (anaerobic metabolism, high tissue oxygen pressure [PtiO 2 ] values). The PtiO 2 profile and its metabolic consequences during anaphylaxis are not known. Methods: Ovalbumin-sensitized anaphylactic shock rats (n ‫؍‬ 11) were compared to nicardipine-induced hypotension rats (n ‫؍‬ 12) for systemic hemodynamics, PtiO 2 , sympathetic nervous system activation, skeletal muscle blood flow, and interstitial lactate and pyruvate concentrations using combined microdialysis and polarographic Clark-type oxygen probes. Results: In both groups, the time course and the magnitude of arterial hypotension were similar. The ovalbumin group but not the nicardipine group displayed decreased skeletal muscle blood flow (from 45 ؎ 6.2 ml ⅐ 100 g ؊1 ⅐ min ؊1 to 24.3

Analysis of Virion Structural Components Reveals Vestiges of the Ancestral Ichnovirus Genome

Many thousands of endoparasitic wasp species are known to inject polydnavirus (PDV) particles into their caterpillar host during oviposition, causing immune and developmental dysfunctions that benefit the wasp larva. PDVs associated with braconid and ichneumonid wasps, bracoviruses and ichnoviruses respectively, both deliver multiple circular dsDNA molecules to the caterpillar. These molecules contain virulence genes but lack core genes typically involved in particle production. This is not completely unexpected given that no PDV replication takes place in the caterpillar. Particle production is confined to the wasp ovary where viral DNAs are generated from proviral copies maintained within the wasp genome. We recently showed that the genes involved in bracovirus particle production reside within the wasp genome and are related to nudiviruses. In the present work we characterized genes involved in ichnovirus particle production by analyzing the components of purified Hyposoter didymator Ichnovirus particles by LC-MS/MS and studying their organization in the wasp genome. Their products are conserved among ichnovirus-associated wasps and constitute a specific set of proteins in the virosphere. Strikingly, these genes are clustered in specialized regions of the wasp genome which are amplified along with proviral DNA during virus particle replication, but are not packaged in the particles. Clearly our results show that ichnoviruses and bracoviruses particles originated from different viral entities, thus providing an example of convergent evolution where two groups of wasps have independently domesticated viruses to deliver genes into their hosts

Extracorporeal Membrane Oxygenation for Severe Acute Respiratory Distress Syndrome associated with COVID-19: An Emulated Target Trial Analysis.

RATIONALE: Whether COVID patients may benefit from extracorporeal membrane oxygenation (ECMO) compared with conventional invasive mechanical ventilation (IMV) remains unknown. OBJECTIVES: To estimate the effect of ECMO on 90-Day mortality vs IMV only Methods: Among 4,244 critically ill adult patients with COVID-19 included in a multicenter cohort study, we emulated a target trial comparing the treatment strategies of initiating ECMO vs. no ECMO within 7 days of IMV in patients with severe acute respiratory distress syndrome (PaO2/FiO2 <80 or PaCO2 ≥60 mmHg). We controlled for confounding using a multivariable Cox model based on predefined variables. MAIN RESULTS: 1,235 patients met the full eligibility criteria for the emulated trial, among whom 164 patients initiated ECMO. The ECMO strategy had a higher survival probability at Day-7 from the onset of eligibility criteria (87% vs 83%, risk difference: 4%, 95% CI 0;9%) which decreased during follow-up (survival at Day-90: 63% vs 65%, risk difference: -2%, 95% CI -10;5%). However, ECMO was associated with higher survival when performed in high-volume ECMO centers or in regions where a specific ECMO network organization was set up to handle high demand, and when initiated within the first 4 days of MV and in profoundly hypoxemic patients. CONCLUSIONS: In an emulated trial based on a nationwide COVID-19 cohort, we found differential survival over time of an ECMO compared with a no-ECMO strategy. However, ECMO was consistently associated with better outcomes when performed in high-volume centers and in regions with ECMO capacities specifically organized to handle high demand. This article is open access and distributed under the terms of the Creative Commons Attribution Non-Commercial No Derivatives License 4.0 (http://creativecommons.org/licenses/by-nc-nd/4.0/)

Prévention - Précaution [Mot à mot]

National audienc

Mise au point d'un nouveau type de spectromètre Raman basé sur l'utilisation d'un DMD, à vocation industrielle

Dans ce travail, nous avons montré la faisabilité du spectromètre Raman de nouvelle génération utilisant un DMD comme modultateur de lumière couplé avec un PM comme détecteur. Le spectromètre donne des résultts très prometteurs pour des analyses qualitatives et quantitaves. Pour les analyses qualitatives il suffit d'une seconde pour identifier un constituant dun mélange. Pour les analyses quantitatives, malgré la présence éventuelle de fluorescence dans les échantillons, les tests effectués sur des mélanges binaires et terniaires de xylènes ont montré qu'on arrive à des erreurs de prédiction d'nviron 3% pour un temps d'analyse de 5 à 6 ssecondespar échantillon. Avec un coût relativement faible, un tel spectromètre, rovuste et précis pourrait être la solution pour de nombreuses nouvelles applications industrielles pour lesquelles, il n'existe pas vraiment de matériel concurent actuellement.Les résultats d'nalyse quantitative utilisan les méthodes chimiompétriques confirment l'efficacité et la précision des méthodes "Backward stepwise selection of Peaks Intensities" (BssPI) et "Sum of Characteristic Peaks of a component" (SCPC).CE SONT DES MÉTHODES SIMPLES, FACILEMENT ACCESSIBLES POUR DES UTILISATEURS NON EXPERTS ET QUI NE DEMANDENT PAS DE CONNAISSANCE PRÉALABLE DES PRODUTIS ANALYSÉS. CES DEUX MÉTHODES SE SONT MONTRÉES ROBUSTES DANS DES CONDITIONS PHYSICO-CHIMIQUES SÉVÈRES QUE L'ON PEUT RENCONTRER DANS UN ENVIRONNEMENT INDUSTRIEL ET S'AVÈRENT AINSI LES MIEUX ADAPTÉES À L'ANANLYSE quantitative à l'aide d'un spectromètre Raman DMD/PM.CHATENAY MALABRY-Ecole centrale (920192301) / SudocSudocFranceF

Etude pharmacocinétique-pharmacodynamique de la quinine chez l'enfant atteint de paludisme à plasmodium falciparum

CHATENAY M.-PARIS 11-BU Pharma. (920192101) / SudocSudocFranceF

Twin pregnancy obtained with frozen-thawed embryos after in vitro maturation in a patient with polycystic ovarian syndrome

Purpose: A twin pregnancy was obtained in a patient with polycystic ovary syndrome after the transfer of three in vitro maturation-derived day 3 embryos that has been frozen and thawed. Methods: The patient had received mild hMG stimulation followed by hCG injection. After culture for 24 - 48 h, mature oocytes were fertilized by ICSI. Embryos were cultured until day 3; supernumerary embryos were cryopreserved using a slow protocol. Results: Among 15 nonatretic oocytes, 9 matured, 8 were fertilized. Four embryos were transferred but they did not implant. The subsequent transfer of three frozen - thawed embryos resulted in the delivery of two healthy girls. Conclusions: These results indicate that a pregnancy could be obtained with in vitro maturation-derived day-3 frozen - thawed embryos

Influence du nombre de pièges à eau colorés en jaune et de la distance entre ces pièges sur les captures d'aphides

International audienc