Altering Social Cue Perception Impacts Honey Bee Aggression With Minimal Impacts on Aggression-Related Brain Gene Expression

Gene expression changes resulting from social interactions may give rise to long term behavioral change, or simply reflect the activity of neural circuitry associated with behavioral expression. In honey bees, social cues broadly modulate aggressive behavior and brain gene expression. Previous studies suggest that expression changes are limited to contexts in which social cues give rise to stable, relatively long-term changes in behavior. Here we use a traditional beekeeping approach that inhibits aggression, smoke exposure, to deprive individuals of aggression-inducing olfactory cues and evaluate whether behavioral changes occur in absence of expression variation in a set of four biomarker genes (drat, cyp6g1/2, GB53860, inos) associated with aggression in previous studies. We also evaluate two markers of a brain hypoxic response (hif1α, hsf) to determine whether smoke induces molecular changes at all. We find that bees with blocked sensory perception as a result of smoke exposure show a strong, temporary inhibition of aggression relative to bees allowed to perceive normal social cues. However, blocking sensory perception had minimal impacts on aggression-relevant gene expression, althought it did induce a hypoxic molecular response in the brain. Results suggest that certain genes differentiate social cue-induced changes in aggression from long-term modulation of this phenotype

Helping Undergraduates Discover The Value Of A Dollar Through Self-Monitoring

Many college undergraduates lack basic financial management knowledge and skills while bearing ever increasing debt burdens upon graduation. In order to encourage students to become aware of their spending patterns and weigh those patterns against personal values, a self-monitoring project was implemented as a class activity. The resulting effect on financial behavior was examined. Analysis of participants’ self-reflection papers revealed that awareness of spending behaviors increased universally among participants, and a significant proportion of students spontaneously modified spending behaviors to more closely conform to personal values. Participants consistently reported the importance of a spending management tool in modifying spending behavior

Impact of Student Debt on Decisions of Terminal Clinical Education Experiences in Physical Therapist Education

Purpose: Student debt is of growing concern in physical therapy and other health professions in the United States. This study explored the extent to which finances and other factors influenced student decision making when applying to and selecting terminal clinical education experiences. Methods: An online survey was developed and distributed to 250 recent graduates of a Doctor of Physical Therapy Program (2016-19). A logistic regression model was used to determine the relationship between respondents’ reported student debt and other factors when choosing a nontraditional model (the yearlong internship model (YLI)/residency model) or the traditional 4-month model. A 5-point Likert scale was used to rate the level of importance for each factor. Results: Seventy-six participants, across four cohorts completed the survey, representing a 30.4% response rate. The logistic regression model found that student debt did not significantly predict whether a student was likely to choose the nontraditional model over the traditional shorter terminal clinical experience; however, age and relationship status were found to be significant predictors of preference for length of experience. Based on aggregate mean data, the most important decision-making criterion in deciding the terminal clinical experience was patient population, followed by the culture of the clinic, then geographic location, and type of clinical setting. The criterion with the lowest aggregate mean was peer (within cohort) competition followed by student debt. A comparison of means found two decision-making criteria significantly reduced from the point of application to the commitment decision: saving money and interprofessional opportunities. Both decision-making criteria became less important for students when deciding on their terminal clinical experience as compared to when they were applying for the terminal clinical experience approximately 3 months earlier. Conclusions / Recommendations: Student debt was not an important factor in selecting the terminal clinical education experience and did not predict whether a student would apply to or select the YLI model rather than the shorter, traditional option. Instead, students considered multiple factors when selecting their terminal experience. Healthcare education programs may want to consider these factors in recruitment of clinical sites, student advising, and/or student placements related to the terminal clinical education experience

Cryptosporidium Parvum-Induced Inflammatory Bowel Disease of TCR-β- x TCR-δ-Deficient Mice

Experimental inoculation of neonatal immunocompetent strains of mice with Cryptosporidium parvum results in a transient, noninflammatory enteric infection. In the present study, we show that inoculation of mice deficient in a 3 and y8 T cells (TCR-3- X TCR-8-deficient mice) with C. parvum results in persistent infection and severe inflammatory bowel disease- like lesions. The most severe lesions in these mice were in the cecum with similar yet less severe lesions in the ileum and proximal colon. The most notable aspect of the histopathology was glandular hyperplasia with abscess formation, extensive fibrosis of the lamina propria with infiltrates of predominately polymorphonuclear cells and macrophages, and a few small aggregates of B cells. Persistently infected mice also developed extensive hepatic periportal fibrosis in association with C. parvum colonization of bile ducts. Lesions observed in TCR- 3- X TCR-8-deficient mice were markedly different than previously described lesions detected in C. parvum-infected TCR-o-deficient mice. Cryptosporidium parvum-infected TCR-o-deficient mice have extensive infiltrations of B cells, whereas TCR-P3- X TCR-8-deficient mice had only a few small aggregates of B cells. These findings indicate that although y8 T cells are not necessary for induction of intestinal inflammation in C. parvum-infected o(4 T- cell-deficient mice, their presence does alter the morphology of the ensuing lesion

Indolinyl-Thiazole Based Inhibitors of Scavenger Receptor-BI (SR-BI)-Mediated Lipid Transport

A potent class of indolinyl-thiazole based inhibitors of cellular lipid uptake mediated by scavenger receptor, class B, type I (SR-BI) was identified via a high-throughput screen of the National Institutes of Health Molecular Libraries Small Molecule Repository (NIH MLSMR) in an assay measuring the uptake of the fluorescent lipid DiI from HDL particles. This class of compounds is represented by ML278 (17–11), a potent (average IC50 = 6 nM) and reversible inhibitor of lipid uptake via SR-BI. ML278 is a plasma-stable, noncytotoxic probe that exhibits moderate metabolic stability, thus displaying improved properties for in vitro and in vivo studies. Strikingly, ML278 and previously described inhibitors of lipid transport share the property of increasing the binding of HDL to SR-BI, rather than blocking it, suggesting there may be similarities in their mechanisms of action

Modelling Eurasian Beaver Foraging Habitat and Dam Suitability, for Predicting the Location and Number of Dams Throughout Catchments in Great Britain

Eurasian beaver (Castor fiber) populations are expanding across Europe. Depending on location, beaver dams bring multiple benefits and/or require management. Using nationally available data, we developed: a Beaver Forage Index (BFI), identifying beaver foraging habitat, and a Beaver Dam Capacity (BDC) model, classifying suitability of river reaches for dam construction, to estimate location and number of dams at catchment scales. Models were executed across three catchments, in Great Britain (GB), containing beaver. An area of 6747 km2 was analysed for BFI and 16,739 km of stream for BDC. Field surveys identified 258 km of channel containing beaver activity and 89 dams, providing data to test predictions. Models were evaluated using a categorical binomial Bayesian framework to calculate probability of foraging and dam construction. BFI and BDC models successfully categorised the use of reaches for foraging and damming, with higher scoring reaches being preferred. Highest scoring categories were ca. 31 and 79 times more likely to be used than the lowest for foraging and damming respectively. Zero-inflated negative binomial regression showed that modelled dam capacity was significantly related (p = 0.01) to observed damming and was used to predict numbers of dams that may occur. Estimated densities of dams, averaged across each catchment, ranged from 0.4 to 1.6 dams/km, though local densities may be up to 30 dams/km. These models provide fundamental information describing the distribution of beaver foraging habitat, where dams may be constructed and how many may occur. This supports the development of policy and management concerning the reintroduction and recolonisation of beaver

The Microarcsecond Sky and Cosmic Turbulence

Radio waves are imprinted with propagation effects from ionized media through which they pass. Owing to electron density fluctuations, compact sources (pulsars, masers, and compact extragalactic sources) can display a wide variety of scattering effects. These scattering effects, particularly interstellar scintillation, can be exploited to provide *superresolution*, with achievable angular resolutions (<~ 1 microarcsecond) far in excess of what can be obtained by very long baseline interferometry on terrestrial baselines. Scattering effects also provide a powerful sub-AU probe of the microphysics of the interstellar medium, potentially to spatial scales smaller than 100 km, as well as a tracer of the Galactic distribution of energy input into the interstellar medium through a variety of integrated measures. Coupled with future gamma-ray observations, SKA observations also may provide a means of detecting fainter compact gamma-ray sources. Though it is not yet clear that propagation effects due to the intergalactic medium are significant, the SKA will either detect or place stringent constraints on intergalactic scattering.Comment: 20 pages, 8 figures in 8 PostScript files, to appear in "Science with the Square Kilometer Array," eds. C. Carilli and S. Rawlings, New Astronomy Reviews (Elsevier: Amsterdam

Salience-based selection: attentional capture by distractors less salient than the target

Current accounts of attentional capture predict the most salient stimulus to be invariably selected first. However, existing salience and visual search models assume noise in the map computation or selection process. Consequently, they predict the first selection to be stochastically dependent on salience, implying that attention could even be captured first by the second most salient (instead of the most salient) stimulus in the field. Yet, capture by less salient distractors has not been reported and salience-based selection accounts claim that the distractor has to be more salient in order to capture attention. We tested this prediction using an empirical and modeling approach of the visual search distractor paradigm. For the empirical part, we manipulated salience of target and distractor parametrically and measured reaction time interference when a distractor was present compared to absent. Reaction time interference was strongly correlated with distractor salience relative to the target. Moreover, even distractors less salient than the target captured attention, as measured by reaction time interference and oculomotor capture. In the modeling part, we simulated first selection in the distractor paradigm using behavioral measures of salience and considering the time course of selection including noise. We were able to replicate the result pattern we obtained in the empirical part. We conclude that each salience value follows a specific selection time distribution and attentional capture occurs when the selection time distributions of target and distractor overlap. Hence, selection is stochastic in nature and attentional capture occurs with a certain probability depending on relative salience