Carcinogenic Ability of Schistosoma Haematobium Possibly through Oncogenic Mutation of KRAS Gene

Schistosoma haematobium is a parasitic flatworm that infects millions of people, mostly in the developing world, and is associated with high incidence of bladder cancer, although why is not clear. Previously, we have used CD-1 mice to show that Schistosoma haematobium total antigen (Sh) has a carcinogenic ability. Sh intravesically instillation induced the development of several urothelial lesions, namely nodular hyperplasia and dysplasia (LGIUN—Low Grade Intra-Urothelial Neoplasia) after 40 weeks of treatment. These results suggested that Sh induce urothelium malignization. Bladder carcinoma frequently harbours gene mutations that constitutively activate the receptor tyrosine kinase-Ras pathway for this reason we studied activating mutations in KRAS gene. Twenty percent of the bladders with dysplasia presented a KRAS mutation in codon 12 of exon 2. We concluded from these results that the parasite extract of S. haematobium has carcinogenic ability possibly through oncogenic mutation of KRAS gene

Single step fabrication of antimicrobial fibre mats from a bioengineered protein-based polymer

Producción CientíficaGenetically engineered protein polymers functionalized with bioactive domains have potential as multifunctional versatile materials for biomedical use. The present work describes the fabrication and characterisation of antimicrobial fibre mats comprising the antimicrobial elastin-like recombinamer (ELR) CM4-A200. The CM4-A200 protein polymer derives from the genetic fusion of the ABP-CM4 antimicrobial peptide from Bombyx mori with 200 repetitions of the pentamer VPAVG. This is the first report on non-crosslinked fibre mats fabricated with an antimicrobial ELR stable in solution. Thermal gravimetric analysis of CM4-A200 fibre mats shows one single degradation step at temperatures above 300 °C, with fibres displaying a higher thermal degradation activation. The electrospun CM4-A200 fibres display high antimicrobial activity against Gram-positive and Gram-negative bacteria with no detectable cytotoxic effects against normal human skin fibroblasts and keratinocytes, revealing the great potential of these polymers for the fabrication of biomedical materials.2018-09-10Ministerio de Economía, Industria y Competitividad (Projects MAT2013-41723-R, MAT2013- 42473-R and MAT2012-38043)Junta de Castilla y León (programa de apoyo a proyectos de investigación – Ref. VA244U13, VA313U14

Granulomatous-like immune reaction and hepatic fibrosis induced by Schistosoma haematobium immature worms

Golden hamsters were inoculated with Schistosoma haematobium cercariae to examine histological lesions at different time points over an 18 month period of infection. Hamsters were sacrificed 26 weeks and 82 weeks after inoculation. The parasite was found in the blood and in the liver of infected animals as was expected, but we found exclusively male worms, no female worms nor eggs. Interestingly we observed unexpected hepatic lesions induced by S. haematobium immature male worms alone in the golden hamster, characteristic of schistosome eggs. Samples from liver, kidneys, lungs, bladder and gastrointestinal tract were collected during necropsy to evaluate injuries induced by S. haematobium. Notably we observed hepatitis in the liver of infected hamsters, no lesions were found in other organs. We also found liver fibrosis in infected hamsters. This study provides further experimental evidence for the role that schistosome worms, and their derived antigens, may play in the pathology of the infection and modulation of liver chronic inflammation in the murine model of schistosomiasis

Protein-Based Films Functionalized with a Truncated Antimicrobial Peptide Sequence Display Broad Antimicrobial Activity

Producción CientíficaThe increasing bacterial resistance to antibiotics is driving strong demand for new antimicrobial biomaterials. This work describes the fabrication of free-standing films exhibiting antimicrobial properties by combining, in the same polypeptide chain, an elastin-like recombinamer comprising 200 repetitions of the pentamer VPAVG (A200) and an 18-amino-acid truncated variant of the antimicrobial peptide BMAP-28, termed BMAP-18. The fusion protein BMAP-18A200 was overexpressed and conveniently purified by a simplified and scalable nonchromatographic process. Free-standing films of BMAP-18A200 demonstrated to be stable without requiring cross-linking agents and displayed high antimicrobial activity against skin pathogens including Gram-negative and Gram-positive bacteria as well as unicellular and filamentous fungi. The antimicrobial activity of the films was mediated by direct contact of cells with the film surface, resulting in compromised structural integrity of microbial cells. Furthermore, the BMAP-18A200 films showed no cytotoxicity on normal human cell lines (skin fibroblasts and keratinocytes). All of these results highlight the potential of these biotechnological multifunctional polymers as new drug-free materials to prevent and treat microbial infections.FCT I.P. (Fundaçao para a Ciencia e Tecnologia, Portugal) and by the European Regional Development Fund (ERDF) through COMPETE2020Programa Operacional Competitividade e Internacionalizacao (POCI, Portugal) in the framework of the Strategic Program UID/BIA/04050/2013 (POCI-01-0145FEDER-007569).program UID/BIA/04050/2019 funded by national funds through FCT I.P.Project EcoAgriFood (NORTE-01-0145-FEDER-000009), supported by Norte Portugal Regional Operational Programme (NORTE 2020

Estimates of major cuts and edible portion of Holstein friesian steer carcasses

Foram utilizadas carcaças de 17 novilhos da raça Holandês PB, criados em confinamento dos 6 aos 24 meses de idade, quando foram abatidos. O presente trabalho teve como objetivo estabelecer equações de regressão, para estimar o peso e a percentagem dos cortes e da porção comestível da carcaça. As variáveis independentes foram: peso vivo, peso da carcaça fria, comprimento da carcaça, comprimento da perna, área do músculo longissimus dorsi, espessura da gordura de cobertura, e conformação; e como variáveis dependentes: peso e percentagem de serrote (lombo, garupa e perna), peso e percentagem de dianteiro (as cinco primeiras costelas), peso e percentagem de costilhar (as últimas oito costelas) e peso e percentagem da porção comestível. O peso da carcaça fria foi a variável que apresentou maior influência no peso dos cortes e da porção comestível, e que as medidas de comprimento, a área do músculo longissimus dorsi e conformação são de baixo valor para estimar o peso e a percentagem dos cortes e da porção comestível.This work involved 17 Holstein steer carcasses, raised in a feed lot from 6 to 24 months of age, when they were slaughtered. The objective of this study was to establish regression equations to estimate the weight and percentage of cuts and of edible portion of the carcass. The independent variables were: live weight, cool carcass weight, carcass length, leg length, longissimus dorsi area, covering fat thickness and conformation; and the dependent variables were: weight and percentage of the socalled saw cut (round, rump and loin), weight and percentage of forequarter (first five ribs), weight and percentage of ribs (last eight), weight and percentage of edible portion. The cool carcass weight was the most important characteristic to esimate weight of cuts and edible portion. Carcass length, conformation, and longissimus dorsi area were found to be measurements of less value for carcass evaluation

Monitoring ten insect pests in selected orchards in three Azorean Islands : The project CUARENTAGRI

BACKGROUND: The data we present are part of the CUARENTAGRI project, which involves all archipelagos of the Macaronesia (Azores, Madeira, Canary Islands and Cabo Verde). The project aims to: i) identify and evaluate the risks associated with the introduction of new arthropod pests; ii) study the population dynamics of selected arthropod pest species currently responsible for the damage of key target crops and iii) develop monitoring systems, based on prediction and/or population dynamics of the crop pests, creating warnings and a phytosanitary prevention system. In this contribution, we compile data for three Azorean Islands (Terceira, São Jorge and São Miguel Islands), where pheromone-baited traps were placed in pastures, potato fields and several orchards’ types (apples, banana, chestnuts, olives, orange and strawberry), during three consecutive years (2020, 2021 and 2022). NEW INFORMATION: A total of 114,827 specimens of insects (Arthropoda, Insecta) were collected, belonging to four orders, six families and ten recorded pest species. A total of eight species are considered introduced (Cosmopolites sordidus (Germar, 1824), Drosophila suzukii (Matsumura, 1931), Bactrocera oleae (Rossi, 1790), Ceratitis capitata (Wiedemann, 1824), Phthorimaea operculella (Zeller, 1873), Cydia pomonella (Linnaeus, 1758), Cydia splendana (Hübner, 1799) and Grapholita molesta (Busck, 1916); n = 84,986 specimens) and two native non-endemic (Mythimna unipuncta (Haworth, 1809) and Spodoptera littoralis (Boisduval, 1833); n = 17,465 specimens). This study intended to contribute to a better knowledge of the arthropods pests that can affect the Azorean crops and will serve as a baseline for future monitoring actions, pest risk assessments and prevention systems.This work was financed under the project CUARENTAGRI by Cooperation Programs INTERREG V A (Spain-Portugal) and MAC 2014-2020. Darwin Core Database management was funded by the Project project FCT-UIDB/00329/2020-2024 (Thematic Line 1 – integrated ecological assessment of environmental change on biodiversity) and Portal da Biodiversidade dos Açores (2022-2023) - PO Azores Project - M1.1.A/INFRAEST CIENT/001/2022. Lucas Lamelas-Lopez was supported by the Project FCT-UIDP/00329/2020-2023.info:eu-repo/semantics/publishedVersio

ELEMENTS OF WINE PRODUCTION IN THE VALLEY OF SAN FRANCISCO BRAZIL - AND COLCHAGUA – CHILE

A videira ou vinha, pertence à família das Vitaceae, subdivididas nas espécies Parthenocissus quinquefólia e Vitis vinífera, sendo esta última utilizada no cultivo de uvas no mundo. Nos próximos anos o consumo de vinho será maior que sua produção, necessitando que a produção da matéria prima seja otimizada. A tecnologia é a ferramenta preponderante nesse processo, trazendo consigo um manejo eficiente a partir da agricultura de precisão. Com intuito de obter indicadores análogos entre as regiões do Vale do São Francisco no Brasil e Vale do Colchagua no Chile, aprimorando o cultivo. Objetiva-se nesse trabalho, caracterizá-las através da análise de seus elementos agronômicos, topográficos, pedológicos e climáticos. O estudo contou com revisão bibliográfica sobre as duas regiões e coleta de dados agrupados em grupos: 1, 2, 3. Onde se discutiu as carências e vantagens agrícolas existentes nas duas regiões, buscando melhoramento do cultivo. Os fatores estudados culminaram numa análise eficiente e provaram sua importância. A agricultura de precisão coloca à disposição da sociedade seus diversos benefícios de forma sustentável