51 research outputs found
Surrealismo y existencialismo : convergencias en la poesía
Víctor José Díaz Goris (biografia): Licenciado en Psicología, en la Universidad Autónoma de Santo Domingo (UASD), 1990. Inglés como segunda lengua (ESL), Universidad APEC, 1991. Maestría en Gerencia de Marketing, Unapec, 2001. Maestría en Educación Superior, Proyecto Unapec-Camagüey, 2005. Posgrado en Educación Virtual, Virtualeduca, 2013. Desde 1991 es docente de la Escuela de Idiomas y desde 2002 imparte docencia de diversas asignaturas del área de Psicología, ambos en Unapec. Como poeta y ensayista ha publicado artículos en las revistas Ágora y Vetas, en la versión digital del periódico Listín Diario y el periódico El Nacional. Ha obtenido numerosos reconocimientos, entre ellos “Poeta destacado del mes” y “Poema destacado del mes”. Su poema “El Quetzal y Tú” obtuvo Mención de Honor en Argentina, sus poemas han sido incluidos en varias antologías internacionales. Fue miembro del grupo de poetas Juglares de la Academia, de la Academia Dominicana de la Lengua.En esta propuesta el autor del texto expone el desarrollo e implicación de dos movimientos conocidos como Surrealismo y Existencialismo y de cómo estas corrientes impactaron otras áreas del saber humano. Además, trata de establecer puntos comunes entre esos dos movimientos, a pesar de su aparente diferencia.Universidad APE
FMD vaccine matching: Inter laboratory study for improved understanding of r1 values
Foot-and-mouth disease virus (FMDV) is a highly variable RNA virus existing as seven different serotypes. The antigenic variability between and within serotypes can limit the cross-reactivity and therefore the in vivo cross-protection of vaccines. Selection of appropriate vaccine strains is crucial in the control of FMD. Determination of indirect relationships (r1-value) between potential vaccine strains and field strains based on antibody responses against both are routinely used for vaccine matching purposes. Aiming at the investigation of the repeatability, reproducibility and comparability of r1-value determination within and between laboratories and serological tests, a small scale vaccine matching ring test for FMDV serotype A was organized. Well-characterized serum pools from cattle vaccinated with a monovalent A24/Cruzeiro/Brazil/55 (A24) FMD vaccine with known in vivo protection status (homologous and heterologous) were distributed to four laboratories to determine r1-values for the heterologous FMD strains A81/Argentina/87, A/Argentina/2000 and A/Argentina/2001 using the virus neutralization tests (VNT) and liquid phase blocking ELISA (LPBE). Within laboratories, the repeatability of r1-value determination was high for both antibody assays. VNT resulted in reproducible and comparable r1-values between laboratories, indicative of a lack of antigenic relatedness between the A24 strain and the heterologous strains tested in this work, thus corresponding to some of the in vivo findings with these strains. Using LPBE, similar trends in r1-values were observed in all laboratories, but the overall reproducibility was lower than with VNT. Inconsistencies between laboratories may at least in part be attributed to differences in LPBE protocols as well as the in preexisting information generated in each laboratory (such as antibody titer-protection correlation curves). To gain more insight in the LPBE-derived r1-values standard bovine control sera were included in the antibody assays performed in each laboratory and a standardization exercise was performed.Fil: Willems, Tom. No especifíca;Fil: De Vleeschauwer, Annebel. No especifíca;Fil: Pérez Filgueira, Daniel Mariano. Instituto Nacional de Tecnologia Agropecuaria. Centro de Investigacion En Ciencias Veterinarias y Agronomicas. Instituto de Virologia E Innovaciones Tecnologicas. - Consejo Nacional de Investigaciones Cientificas y Tecnicas. Oficina de Coordinacion Administrativa Pque. Centenario. Instituto de Virologia E Innovaciones Tecnologicas.; ArgentinaFil: Li, Yanmin. No especifíca;Fil: Ludi, Anna. No especifíca;Fil: Lefebvre, David. No especifíca;Fil: Wilsden, Ginette. No especifíca;Fil: Statham, Bob. No especifíca;Fil: Haas, Bernd. Federal Research Institute for Animal Health; AlemaniaFil: Mattion, Nora Marta. Ministerio de Produccion y Trabajo. Secretaria de Gobierno de Agroindustria. Servicio Nacional de Sanidad y Calidad Agroalimentaria. Centro de Virologia Animal. - Consejo Nacional de Investigaciones Cientificas y Tecnicas. Oficina de Coordinacion Administrativa Ciudad Universitaria. Centro de Virologia Animal.; ArgentinaFil: Robiolo, Blanca. Ministerio de Produccion y Trabajo. Secretaria de Gobierno de Agroindustria. Servicio Nacional de Sanidad y Calidad Agroalimentaria. Centro de Virologia Animal. - Consejo Nacional de Investigaciones Cientificas y Tecnicas. Oficina de Coordinacion Administrativa Ciudad Universitaria. Centro de Virologia Animal.; ArgentinaFil: Beascoechea Perez, Claudia. Ministerio de Agricultura, Ganadería, Pesca y Alimento. Servicio Nacional de Sanidad y Calidad Agroalimentaria; ArgentinaFil: Maradei, Eduardo. Ministerio de Agricultura, Ganadería, Pesca y Alimento. Servicio Nacional de Sanidad y Calidad Agroalimentaria; ArgentinaFil: Smitsaart, Eliana. Biogénesis Bagó; ArgentinaFil: la Torre, Jose Leonardo. Ministerio de Produccion y Trabajo. Secretaria de Gobierno de Agroindustria. Servicio Nacional de Sanidad y Calidad Agroalimentaria. Centro de Virologia Animal. - Consejo Nacional de Investigaciones Cientificas y Tecnicas. Oficina de Coordinacion Administrativa Ciudad Universitaria. Centro de Virologia Animal.; ArgentinaFil: De Clercq, Kris. No especifíca
Phase transformation of superparamagnetic iron oxide nanoparticles via thermal annealing: implications for hyperthermia applications
Magnetic hyperthermia has the potential to play an important role in cancer therapy and its efficacy relies on the nanomaterials selected. Superparamagnetic iron oxide nanoparticles (SPIONs) are excellent candidates due to the ability of producing enough heat to kill tumor cells by thermal ablation. However, their heating properties depend strongly on crystalline structure and size, which may not be controlled and tuned during the synthetic process; therefore, a postprocessing is needed. We show how thermal annealing can be simultaneously coupled with ligand exchange to stabilize the SPIONs in polar solvents and to modify their crystal structure, which improves hyperthermia behavior. Using high-resolution transmission electron microscopy, X-ray diffraction, Mössbauer spectroscopy, vibrating sample magnetometry, and lock-in thermography, we systematically investigate the impact of size and ligand exchange procedure on crystallinity, their magnetism, and heating ability. We describe a valid and simple approach to optimize SPIONs for hyperthermia by carefully controlling the size, colloidal stability, and crystallinity
Genetic variability of histamine receptors in patients with Parkinson's disease
<p>Abstract</p> <p>Background</p> <p>Changes in the density and expression of histamine receptors (HRH) have been detected in Parkinson's disease (PD) patients, and HRH antagonists bring about improvements in motor and other symptoms, thus suggesting that HRH play a role in the clinical response of PD patients. This study is aimed to analyse polymorphic variations of HRH in patients with PD.</p> <p>Methods</p> <p>Leukocytary DNA from 195 PD patients and a control group of 231 unrelated healthy individuals was studied for the nonsynonymous HRH1Leu449Ser and the promoter HRH2G-1018A polymorphisms by using amplification-restriction analyses.</p> <p>Results</p> <p>The HRH1Leu449Ser amino acid substitution was identified in two women with late-onset PD whereas it was not observed among healthy subjects. The HRH2G-1018A polymorphism was observed with allele frequencies = 3.59 (95% CI = 1.74–5.44) and 5.0 (95% CI = 3.00–6.96) for patients with PD and healthy controls, respectively. These frequencies were independent of gender and age of onset of the disease. Multiple comparison analyses revealed that differences were not statistically significant.</p> <p>Conclusion</p> <p>These results indicate that the polymorphisms analyzed are not a major risk factor for PD, although the HRH1Leu449Ser amino acid substitution might be related to PD.</p
Latent tuberculosis infection, tuberculin skin test and vitamin D status in contacts of tuberculosis patients: a cross-sectional and case-control study
<p>Abstract</p> <p>Background</p> <p>Deficient serum vitamin D levels have been associated with incidence of tuberculosis (TB), and latent tuberculosis infection (LTBI). However, to our knowledge, no studies on vitamin D status and tuberculin skin test (TST) conversion have been published to date. The aim of this study was to estimate the associations of serum 25-hydroxyvitamin D<sub>3 </sub>(25[OH]D) status with LTBI prevalence and TST conversion in contacts of active TB in Castellon (Spain).</p> <p>Methods</p> <p>The study was designed in two phases: cross-sectional and case-control. From November 2009 to October 2010, contacts of 42 TB patients (36 pulmonary, and 6 extra-pulmonary) were studied in order to screen for TB. LTBI and TST conversion cases were defined following TST, clinical, analytic and radiographic examinations. Serum 25(OH)D levels were measured by electrochemiluminescence immunoassay (ECLIA) on a COBAS<sup>® </sup>410 ROCHE<sup>® </sup>analyzer. Logistic regression models were used in the statistical analysis.</p> <p>Results</p> <p>The study comprised 202 people with a participation rate of 60.1%. Only 20.3% of the participants had a sufficient serum 25(OH)D (≥ 30 ng/ml) level. In the cross-sectional phase, 50 participants had LTBI and no association between LTBI status and serum 25(OH)D was found. After 2 months, 11 out of 93 negative LTBI participants, without primary prophylaxis, presented TST conversion with initial serum 25(OH)D levels: a:19.4% (7/36): < 20 ng/ml, b:12.5% (4/32):20-29 ng/ml, and c:0%(0/25) ≥ 30 ng/ml. A sufficient serum 25(OH)D level was a protector against TST conversion a: Odds Ratio (OR) = 1.00; b: OR = 0.49 (95% confidence interval (CI) 0.07-2.66); and c: OR = 0.10 (95% CI 0.00-0.76), trends p = 0.019, adjusted for high exposure and sputum acid-fast bacilli positive index cases. The mean of serum level 25(OH)D in TST conversion cases was lower than controls,17.5 ± 5.6 ng/ml versus 25.9 ± 13.7 ng/ml (p = 0.041).</p> <p>Conclusions</p> <p>The results suggest that sufficient serum 25(OH)D levels protect against TST conversion.</p
Assembling the Marine Metagenome, One Cell at a Time
The difficulty associated with the cultivation of most microorganisms and the complexity of natural microbial assemblages, such as marine plankton or human microbiome, hinder genome reconstruction of representative taxa using cultivation or metagenomic approaches. Here we used an alternative, single cell sequencing approach to obtain high-quality genome assemblies of two uncultured, numerically significant marine microorganisms. We employed fluorescence-activated cell sorting and multiple displacement amplification to obtain hundreds of micrograms of genomic DNA from individual, uncultured cells of two marine flavobacteria from the Gulf of Maine that were phylogenetically distant from existing cultured strains. Shotgun sequencing and genome finishing yielded 1.9 Mbp in 17 contigs and 1.5 Mbp in 21 contigs for the two flavobacteria, with estimated genome recoveries of about 91% and 78%, respectively. Only 0.24% of the assembling sequences were contaminants and were removed from further analysis using rigorous quality control. In contrast to all cultured strains of marine flavobacteria, the two single cell genomes were excellent Global Ocean Sampling (GOS) metagenome fragment recruiters, demonstrating their numerical significance in the ocean. The geographic distribution of GOS recruits along the Northwest Atlantic coast coincided with ocean surface currents. Metabolic reconstruction indicated diverse potential energy sources, including biopolymer degradation, proteorhodopsin photometabolism, and hydrogen oxidation. Compared to cultured relatives, the two uncultured flavobacteria have small genome sizes, few non-coding nucleotides, and few paralogous genes, suggesting adaptations to narrow ecological niches. These features may have contributed to the abundance of the two taxa in specific regions of the ocean, and may have hindered their cultivation. We demonstrate the power of single cell DNA sequencing to generate reference genomes of uncultured taxa from a complex microbial community of marine bacterioplankton. A combination of single cell genomics and metagenomics enabled us to analyze the genome content, metabolic adaptations, and biogeography of these taxa
Objectively measured physical activity in european adults: cross-sectional findings from the Food4Me study
Introduction
Physical inactivity has been estimated to be responsible for more than 5.3 million deaths worldwide
[1]. Moreover, among European men and women, approximately 7.3% of all deaths in
2008 might be attributable to inactivity compared with 3.7% to obesity [2] and there is strong
evidence to suggest that even small increases in physical activity (PA) would lower the risk for
many non-communicable diseases [1–3]. Yet, levels of PA across populations remain low [4].
To tackle this public health issue, the US Centers for Disease Control and Prevention and the
American College of Sports Medicine produced standardized PA guidelines 20 years ago [5].
Since then, the World Health Organization (WHO), the European Union, and most countries
around the world, have included PA guidelines in their health policies. Guidelines for Americans
and Europeans have been updated to include recommendations for adolescents and for
older adults [6–9]. For adults aged 18–64 years old, the WHO recommends a minimum of 150
min of moderate intensity PA per week, 75 min of vigorous intensity PA or an equivalent
amount of moderate and vigorous PA (MVPA) [9].
In 2008, 34.8% of adults 15 years or older were insufficiently active in Europe [4]. Regular
surveillance is needed to update these prevalence estimates and to evaluate the effectiveness of
PA policies and promotion programs in European countries. In this context, the objective
assessment of PA is a key issue. Prevalence of physical inactivity has been mainly derived from
self-reported measures such as the Baecke questionnaire [10] or the International Physical
Activity Questionnaire (IPAQ) [11]. These questionnaires have been, and still are, widely used
due to their simple administration and low cost [12]. However, PA is frequently misreported,
which leads to considerable measurement error [13–15]. Accelerometers offer a potential solution
because they measure PA objectively. Given that they are small and easy to wear, store
data up to several weeks and are acceptable in terms of reliability, these devices are now used
increasingly in large studies to assess PA in children, adolescents and adults [16]. Although
some European countries have reported adherence to PA guidelines using accelerometers in
large cohorts [17–19], comparisons between European countries measured according to the
same standardized protocols and concurrently are lacking.
Between 2012 and 2014, PA was assessed objectively by accelerometry in the participants of
the Food4Me Proof-of-Principle (PoP) study. The Food4Me Study was a web-based randomized
controlled trial on personalized nutrition, across seven European countries: Germany,
Greece, Ireland, The Netherlands, Poland, Spain and the United Kingdom. The aim of the current
paper is to describe and compare PA in adults from these countries, and evaluate adherence
to PA guidelines, using baseline data from the Food4Me PoP study
Effects of Satisfaction with Communication on the Relationship Between Individual-Job Congruence and Job Performance/Satisfaction
Purpose - The purpose of this paper is to examine the moderating influence of communication satisfaction on the association between individual-job congruence and both job performance and job satisfaction. Design/methodology/approach - Moderated regression analysis was used to assess data collected from 302 employees addressing the research variables of job scope, growth need strength, satisfaction with communication, job performance, and job satisfaction. Findings - Satisfaction with communication received weak support as a moderator of the individual-job congruence model; nevertheless, it received strong support as a main predictor of both performance and satisfaction. Research limitations/implications - Low statistical power frequently reflected by moderated regression analysis may explain the weak support communication satisfaction received as a moderator. Different approaches for solving the presence of low power are discussed. On the other hand, the elusive venture of promoting and experiencing satisfaction with communication has been detected and the need for exploring the possible curvilinear effects of specific communication dimensions and organizational constructs on communication satisfaction is introduced. Practical implications - Variables associated with human interaction may be dysfunctional at both extremes. For example, upward communication might have favorable and unfavorable consequences on satisfaction with communication. Thus, dealing with communication satisfaction may necessitate the adoption of a contingency approach. Originality/value - The uniqueness of this research lies in its effort of exploring the moderating impact of communication satisfaction on the job characteristics model. The results encourage future research endeavors and particular management practices
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