19 research outputs found
A multi-omics analysis of the grapevine pathogen Lasiodiplodia theobromae reveals that temperature affects the expression of virulence- and pathogenicity-related genes
Lasiodiplodia theobromae (Botryosphaeriaceae, Ascomycota) is a plant pathogen and human opportunist whose pathogenicity is modulated by temperature. The molecular effects of temperature on L. theobromae are mostly unknown, so we used a multi-omics approach to understand how temperature affects the molecular mechanisms of pathogenicity. The genome of L. theobromae LA-SOL3 was sequenced (Illumina MiSeq) and annotated. Furthermore, the transcriptome (Illumina TruSeq) and proteome (Orbitrap LC-MS/MS) of LA-SOL3 grown at 25 degrees C and 37 degrees C were analysed. Proteins related to pathogenicity (plant cell wall degradation, toxin synthesis, mitogen-activated kinases pathway and proteins involved in the velvet complex) were more abundant when the fungus grew at 25 degrees C. At 37 degrees C, proteins related to pathogenicity were less abundant than at 25 degrees C, while proteins related to cell wall organisation were more abundant. On the other hand, virulence factors involved in human pathogenesis, such as the SSD1 virulence protein, were expressed only at 37 degrees C. Taken together, our results showed that this species presents a typical phytopathogenic molecular profile that is compatible with a hemibiotrophic lifestyle. We showed that L. theobromae is equipped with the pathogenesis toolbox that enables it to infect not only plants but also animals
Plant Proteomic Research 3.0: Challenges and Perspectives
Advancements in high-throughput “Omics” techniques have revolutionized plant molecular biology research [...
Plant Proteomic Research 3.0: Challenges and Perspectives
Advancements in high-throughput “Omics” techniques have revolutionized plant molecular biology research [...
From 2003 to 2011: Proteomics investigation at the agroforestry and plant biochemistry and proteomics research group (University of Cordoba, Spain)
International audienc
From 2003 to 2011: Proteomics investigation at the agroforestry and plant biochemistry and proteomics research group (University of Cordoba, Spain)
International audienc
Molecular responses to ionising radiation exposure in Arabidopsis thaliana
International audienc
Molecular responses to ionising radiation exposure in Arabidopsis thaliana
International audienc
Application of Label-Free Shotgun nUPLC–MS<sup>E</sup> and 2‑DE Approaches in the Study of <i>Botrytis cinerea</i> Mycelium
The phytopathogenic fungus <i>Botrytis cinerea</i> infects
more than different 200 plant species and causes substantial losses
in numerous crops. The B05.10 and T4 wild-type strain genomes have
been recently sequenced, becoming a model system for necrotrophic
pathogens, as well as opening up new alternatives in functional genomics,
such as proteomics. We analyzed <i>B. cinerea</i> mycelium
from these two wild-type strains, introducing label-free shotgun nUPLC–MS<sup>E</sup> methodology to complement the 2-DE-MS-based approach. We
assessed the label-free nUPLC–MS<sup>E</sup> methodology for
protein identification and quantification using five mycelium protein
dilutions. A total of 225 and 170 protein species were identified
by nUPLC–MS<sup>E</sup> in the B05.10 and T4 strains, respectively.
Moreover, 129 protein species were quantified in both strains. Significant
differences in protein abundance were found in 15 more abundant and
16 less abundant protein species in the B05.10 strain compared to
the T4 strain. Twenty-nine qualitative and 15 significant quantitative
differences were found using 2-DE. The label-free nUPLC–MS<sup>E</sup> was a reliable, reproducible and sensitive method for protein
identification and quantification to study the <i>B. cinerea</i> mycelial proteome. Results obtained by gel-based and gel-free complementary
approaches allow a deeper characterization of this fungus, as well
as the identification of potential virulence factors
International Plant Proteomics Organization (INPPO) World Congress 2014
The field of proteomics has advanced considerably over the past two decades. The ability to delve deeper into an organism’s proteome, identify an array of post-translational modifications and profile differentially abundant proteins has greatly expanded the utilization of proteomics. Improvements to instrumentation in conjunction with the development of these reproducible workflows have driven the adoption and application of this technology by a wider research community. However, the full potential of proteomics is far from being fully exploited in plant biology and its translational application needs to be further developed. In 2011, a group of plant proteomic researchers established the International Plant Proteomics Organization (INPPO) to advance the utilization of this technology in plants as well as to create a way for plant proteomics researchers to interact, collaborate and exchange ideas. The INPPO conducted its inaugural world congress in mid 2014 at the University of Hamburg (Germany). Plant proteomic researchers from around the world were in attendance and the event marked the maturation of this research community. The Research Topic captures the opinions, ideas and research discussed at the congress and encapsulates the approaches that were being applied in plant proteomics.The field of proteomics has advanced considerably over the past two decades. The ability to delve deeper into an organism’s proteome, identify an array of post-translational modifications and profile differentially abundant proteins has greatly expanded the utilization of proteomics. Improvements to instrumentation in conjunction with the development of these reproducible workflows have driven the adoption and application of this technology by a wider research community. However, the full potential of proteomics is far from being fully exploited in plant biology and its translational application needs to be further developed. In 2011, a group of plant proteomic researchers established the International Plant Proteomics Organization (INPPO) to advance the utilization of this technology in plants as well as to create a way for plant proteomics researchers to interact, collaborate and exchange ideas. The INPPO conducted its inaugural world congress in mid 2014 at the University of Hamburg (Germany). Plant proteomic researchers from around the world were in attendance and the event marked the maturation of this research community. The Research Topic captures the opinions, ideas and research discussed at the congress and encapsulates the approaches that were being applied in plant proteomics