A multi-omics analysis of the grapevine pathogen Lasiodiplodia theobromae reveals that temperature affects the expression of virulence- and pathogenicity-related genes

Lasiodiplodia theobromae (Botryosphaeriaceae, Ascomycota) is a plant pathogen and human opportunist whose pathogenicity is modulated by temperature. The molecular effects of temperature on L. theobromae are mostly unknown, so we used a multi-omics approach to understand how temperature affects the molecular mechanisms of pathogenicity. The genome of L. theobromae LA-SOL3 was sequenced (Illumina MiSeq) and annotated. Furthermore, the transcriptome (Illumina TruSeq) and proteome (Orbitrap LC-MS/MS) of LA-SOL3 grown at 25 degrees C and 37 degrees C were analysed. Proteins related to pathogenicity (plant cell wall degradation, toxin synthesis, mitogen-activated kinases pathway and proteins involved in the velvet complex) were more abundant when the fungus grew at 25 degrees C. At 37 degrees C, proteins related to pathogenicity were less abundant than at 25 degrees C, while proteins related to cell wall organisation were more abundant. On the other hand, virulence factors involved in human pathogenesis, such as the SSD1 virulence protein, were expressed only at 37 degrees C. Taken together, our results showed that this species presents a typical phytopathogenic molecular profile that is compatible with a hemibiotrophic lifestyle. We showed that L. theobromae is equipped with the pathogenesis toolbox that enables it to infect not only plants but also animals

Plant Proteomic Research 3.0: Challenges and Perspectives

Advancements in high-throughput “Omics” techniques have revolutionized plant molecular biology research [...

Plant Proteomic Research 3.0: Challenges and Perspectives

Advancements in high-throughput “Omics” techniques have revolutionized plant molecular biology research [...

From 2003 to 2011: Proteomics investigation at the agroforestry and plant biochemistry and proteomics research group (University of Cordoba, Spain)

International audienc

From 2003 to 2011: Proteomics investigation at the agroforestry and plant biochemistry and proteomics research group (University of Cordoba, Spain)

International audienc

Molecular responses to ionising radiation exposure in Arabidopsis thaliana

International audienc

Molecular responses to ionising radiation exposure in Arabidopsis thaliana

International audienc

Proteomics to unravel mechanisms of nanoparticle toxicity

International audienc

Application of Label-Free Shotgun nUPLC–MS^E and 2‑DE Approaches in the Study of <i>Botrytis cinerea</i> Mycelium

The phytopathogenic fungus <i>Botrytis cinerea</i> infects more than different 200 plant species and causes substantial losses in numerous crops. The B05.10 and T4 wild-type strain genomes have been recently sequenced, becoming a model system for necrotrophic pathogens, as well as opening up new alternatives in functional genomics, such as proteomics. We analyzed <i>B. cinerea</i> mycelium from these two wild-type strains, introducing label-free shotgun nUPLC–MS^E methodology to complement the 2-DE-MS-based approach. We assessed the label-free nUPLC–MS^E methodology for protein identification and quantification using five mycelium protein dilutions. A total of 225 and 170 protein species were identified by nUPLC–MS^E in the B05.10 and T4 strains, respectively. Moreover, 129 protein species were quantified in both strains. Significant differences in protein abundance were found in 15 more abundant and 16 less abundant protein species in the B05.10 strain compared to the T4 strain. Twenty-nine qualitative and 15 significant quantitative differences were found using 2-DE. The label-free nUPLC–MS^E was a reliable, reproducible and sensitive method for protein identification and quantification to study the <i>B. cinerea</i> mycelial proteome. Results obtained by gel-based and gel-free complementary approaches allow a deeper characterization of this fungus, as well as the identification of potential virulence factors

International Plant Proteomics Organization (INPPO) World Congress 2014

The field of proteomics has advanced considerably over the past two decades. The ability to delve deeper into an organism’s proteome, identify an array of post-translational modifications and profile differentially abundant proteins has greatly expanded the utilization of proteomics. Improvements to instrumentation in conjunction with the development of these reproducible workflows have driven the adoption and application of this technology by a wider research community. However, the full potential of proteomics is far from being fully exploited in plant biology and its translational application needs to be further developed. In 2011, a group of plant proteomic researchers established the International Plant Proteomics Organization (INPPO) to advance the utilization of this technology in plants as well as to create a way for plant proteomics researchers to interact, collaborate and exchange ideas. The INPPO conducted its inaugural world congress in mid 2014 at the University of Hamburg (Germany). Plant proteomic researchers from around the world were in attendance and the event marked the maturation of this research community. The Research Topic captures the opinions, ideas and research discussed at the congress and encapsulates the approaches that were being applied in plant proteomics.The field of proteomics has advanced considerably over the past two decades. The ability to delve deeper into an organism’s proteome, identify an array of post-translational modifications and profile differentially abundant proteins has greatly expanded the utilization of proteomics. Improvements to instrumentation in conjunction with the development of these reproducible workflows have driven the adoption and application of this technology by a wider research community. However, the full potential of proteomics is far from being fully exploited in plant biology and its translational application needs to be further developed. In 2011, a group of plant proteomic researchers established the International Plant Proteomics Organization (INPPO) to advance the utilization of this technology in plants as well as to create a way for plant proteomics researchers to interact, collaborate and exchange ideas. The INPPO conducted its inaugural world congress in mid 2014 at the University of Hamburg (Germany). Plant proteomic researchers from around the world were in attendance and the event marked the maturation of this research community. The Research Topic captures the opinions, ideas and research discussed at the congress and encapsulates the approaches that were being applied in plant proteomics