3,537 research outputs found
Fixing DNA breaks during class switch recombination
Immunoglobulin (Ig) class switch recombination (CSR) involves the breakage and subsequent repair of two DNA sequences, known as switch (S) regions, which flank IgH constant region exons. The resolution of CSR-associated breaks is thought to require the nonhomologous end-joining (NHEJ) DNA repair pathway, but the role of the NHEJ factor DNA-dependent protein kinase catalytic subunit (DNA-PKcs) in this process has been unclear. A new study, in which broken IgH-containing chromosomes in switching B cells were visualized directly, clearly demonstrated that DNA-PKcs and, unexpectedly, the nuclease Artemis are involved in the resolution of switch breaks
Hyperbolicity and the effective dimension of spatially-extended dissipative systems
We show, using covariant Lyapunov vectors, that the chaotic solutions of
spatially extended dissipative systems evolve within a manifold spanned by a
finite number of physical modes hyperbolically isolated from a set of residual
degrees of freedom, themselves individually isolated from each other. In the
context of dissipative partial differential equations, our results imply that a
faithful numerical integration needs to incorporate at least all physical modes
and that increasing the resolution merely increases the number of isolated
modes.Comment: 4 pages, 4 figure
Thermodiffusion in model nanofluids by molecular dynamics simulations
In this work, a new algorithm is proposed to compute single particle
(infinite dilution) thermodiffusion using Non-Equilibrium Molecular Dynamics
simulations through the estimation of the thermophoretic force that applies on
a solute particle. This scheme is shown to provide consistent results for
simple Lennard-Jones fluids and for model nanofluids (spherical non-metallic
nanoparticles + Lennard-Jones fluid) where it appears that thermodiffusion
amplitude, as well as thermal conductivity, decrease with nanoparticles
concentration. Then, in nanofluids in the liquid state, by changing the nature
of the nanoparticle (size, mass and internal stiffness) and of the solvent
(quality and viscosity) various trends are exhibited. In all cases the single
particle thermodiffusion is positive, i.e. the nanoparticle tends to migrate
toward the cold area. The single particle thermal diffusion 2 coefficient is
shown to be independent of the size of the nanoparticle (diameter of 0.8 to 4
nm), whereas it increases with the quality of the solvent and is inversely
proportional to the viscosity of the fluid. In addition, this coefficient is
shown to be independent of the mass of the nanoparticle and to increase with
the stiffness of the nanoparticle internal bonds. Besides, for these
configurations, the mass diffusion coefficient behavior appears to be
consistent with a Stokes-Einstein like law
High Resolution HST-STIS Spectra of CI and CO in the Beta Pictoris Circumstellar Disk
High resolution FUV echelle spectra showing absorption features arising from
CI and CO gas in the Beta Pictoris circumstellar (CS) disk were obtained on
1997 December 6 and 19 using the Space Telescope Imaging Spectrograph (STIS).
An unsaturated spin-forbidden line of CI at 1613.376 A not previously seen in
spectra of Beta Pictoris was detected, allowing for an improved determination
of the column density of CI at zero velocity relative to the star (the stable
component), N = (2-4) x 10^{16} cm^{-2}. Variable components with multiple
velocities, which are the signatures of infalling bodies in the Beta Pictoris
CS disk, are observed in the CI 1561 A and 1657 A multiplets. Also seen for the
first time were two lines arising from the metastable singlet D level of
carbon, at 1931 A and 1463 A The results of analysis of the CO A-X (0-0),
(1-0), and (2-0) bands are presented, including the bands arising from {13}^CO,
with much better precision than has previously been possible, due to the very
high resolution provided by the STIS echelle gratings. Only stable CO gas is
observed, with a column density N(CO) = (6.3 +/- 0.3) x 10^{14} cm{-2}. An
unusual ratio of the column densities of {12}^CO to {13}^CO is found (R = 15
+/- 2). The large difference between the column densities of CI and CO
indicates that photodissociation of CO is not the primary source of CI gas in
the disk, contrary to previous suggestion.Comment: 13 pages, including 6 figures. LaTex2e (emulateapj5.sty). Accepted
for publication in Ap
From meadows to milk to mucosa – adaptation of Streptococcus and Lactococcus species to their nutritional environments
Lactic acid bacteria (LAB) are indigenous to food-related habitats as well as associated with the mucosal surfaces of animals. The LAB family Streptococcaceae consists of the genera Lactococcus and Streptococcus. Members of the family include the industrially important species Lactococcus lactis, which has a long history safe use in the fermentative food industry, and the disease-causing streptococci Streptococcus pneumoniae and Streptococcus pyogenes. The central metabolic pathways of the Streptococcaceae family have been extensively studied because of their relevance in the industrial use of some species, as well as their influence on virulence of others. Recent developments in high-throughput proteomic and DNA-microarray techniques, in in vivo NMR studies, and importantly in whole-genome sequencing have resulted in new insights into the metabolism of the Streptococcaceae family. The development of cost-effective high-throughput sequencing has resulted in the publication of numerous whole-genome sequences of lactococcal and streptococcal species. Comparative genomic analysis of these closely related but environmentally diverse species provides insight into the evolution of this family of LAB and shows that the relatively small genomes of members of the Streptococcaceae family have been largely shaped by the nutritionally rich environments they inhabit.
The Relationship Between the Producer-Consumer Problem and Redundancy
Many system administrators would agree that, had it not been for the development of congestion control, the simulation of linked lists might never have occurred. In fact, few mathematicians would disagree with the construction of IPv4. In this paper we examine how sensor networks can be applied to the evaluation of symmetric encryption
Early markers of prolonged hospital stay in demented inpatients: A multicentre and prospective study
Background: Dementia is a serious, chronic, and costly public health problem. Prior studies have described dementia as increasing length of hospital stay, but so far no explanations have been proposed. Methods: To identify early markers for prolonged hospital stay in demented elderly inpatients, 178 community-dwelling or institutionalized subjects aged 75+, hospitalized through an emergency department in 9 teaching hospitals in France, were analyzed. Prolonged hospital stays were defined according a limit adjusted for Diagnosis Related Group. All patients underwent a comprehensive geriatric assessment at admission. Logistic regression multifactorial mixed model was performed. Center effect was considered as a random effect. Results: Of the 178 stays, 52 were prolonged. Most concerned community-dwelling patients (86%). Multifactor analysis demonstrated that demographic variables had no influence on the length of stay, while diagnosis of delirium (OR 2.31; 95% CI 1.77-2.91), walking difficulties (OR 1.94; 95% CI 1.62-2.43) and report by the informal caregiver of moderate or severe burden (OR 1.52; 95% CI 1.19-1.86) or low social quality-of-life score (OR 1.25; 95% CI 1.03-1.40), according to the Zarit's Burden Inventory short scale (12 items) and the Duke's Health Profile respectively, were identified as early markers for prolonged hospital stays. Conclusion: At the time of the rising incidence of cognitive disorders, these results suggest that preventive approaches might be possible. In a hospital setting as well as in a community-dwelling population, more specific, specialized and coordinated care, using the expertise of multiple disciplines appears as a probable effective measure to limit prolonged hospital stay. Such approaches require (i) clear patient-oriented goal definition, (ii) understanding and appreciation of roles among various health care and social disciplines and, (iii) cooperation between partners in patient's management. However, the cost- and health-effectiveness of such approaches should be evaluate
The use of a multifactorial approach to reduce Salmonella shedding
An analytic epidemiological survey was carried out in france to assess the circumstances for Salmonella shedding in finishing pigs. This study gave out a combination of risk factors. The aim of our study was to evaluate that the implementation of a programme based on these risk factors reduces Salmonella carriage
- …