Weak relationships between injuries in freezing tests and performance in short-term and field trials of Norway spruce families from Stange Seed Orchard

Artificial freezing tests were performed on seedlings from Norway spruce families at the end of the first growing season. Similar tests were made on twigs collected from trees in a progeny test at the end of growing season nine. The 26 families in the early test were included in the short-term progeny test with 100 full-sib families from a 10 x 10 factorial cross. All families were also planted in seven field trials in Norway, Sweden and Finland, from which data on mortality, tree heights and stem damage at age 10 years are available. Significant difference was found among families for freezing test injuries on whole intact seedlings at the end of the first growing season and for lethal temperature of needles on detached twigs collected at the end of growing season nine. However, no relationships were found between the freezing test scores of families in the two types of tests or few between these scores and the traits measured in the short-term and field trials. The results show that frost hardiness testing of families at a young age, grown under artificial temperature and light conditions in nursery, is a weak predictor of their performance under natural conditions in field at older ages.Weak relationships between injuries in freezing tests and performance in short-term and field trials of Norway spruce families from Stange Seed OrchardWeak relationships between injuries in freezing tests and performance in short-term and field trials of Norway spruce families from Stange Seed OrchardpublishedVersio

Pharmacodynamic mechanisms behind a refractory state in inflammatory bowel disease

Background and aims - Biological therapy for inflammatory bowel disease is efficient in many cases but not all. The underlying molecular mechanisms behind non-response to biological therapy in inflammatory bowel disease are poorly described. Therefore, we aimed to characterize the mucosal cytokine transcript profile in non-immunogenic, non-responder patients with adequate trough level. Material and methods - Patients with ulcerative colitis (UC) (n = 21) and Crohn’s disease (CD) (n = 12) with non-response to biological therapy (anti-tumor necrosis factor (TNF) or vedolizumab) were included. Reference groups were A: untreated patients with UC or CD at debut of disease who had severe 1-year outcome, B: patients with UC or CD treated to endoscopic remission with biological agents, and C: healthy normal controls. Mucosal transcripts of TNF, interleukin (IL)17 and IL23 were measured by reverse transcription real-time quantitative polymerase chain reaction. Results Of the non-responders, 2 out of 12 CD and 1 out of 21 UC patients needed surgery during follow-up. Of the remaining non-responding patients, 8 out of 10 CD and 12 out of 20 UC patients switched biologic treatment. The remaining 2 CD and 8 UC patients continued treatment with the same biological agent with the addition of steroids, immunomodulators (AZA/MTX) and /or local steroids/5ASA. Twelve (8 UC/4 CD) out of 20 IBD patients were still non-responders after changing biological therapy to either anti-TNF (2), vedolizumab (9) or ustekinumab (1). The transcripts of IL17, IL23 and TNF were significantly upregulated in the non-response group compared to normal controls and patients in remission. In UC, 24% of the non-responders had normal mucosal TNF transcript indicating a non-TNF mediated inflammation. No obvious differences in gene expression were observed between primary and secondary non-responders, nor between anti-TNF and vedolizumab non-responders. Conclusions - Mucosal transcripts of IL17 and IL23 are highly associated with non-response to biological therapy, whereas some UC patients may also have a non-TNF mediated inflammatory pathway

Migration of Atlantic salmon post-smolts in a fjord with high infestation pressure of salmon lice

Understanding Atlantic salmon Salmo salar post-smolt coastal migration behaviour is crucial for predicting their exposure to ecological challenges such as the parasite salmon louse Lepeophtheirus salmonis. We compared the migration of acoustically tagged, hatchery-reared Atlantic salmon post-smolts of wild and domesticated origins from the inner, middle and outer part of a 172 km long aquaculture-intensive fjord in western Norway. Additionally, we examined if the timing of the release or treatment with an anti-parasitic drug (prophylaxis) altered migratory behaviour. We found no significant differences in mean progression rates among the 3 release locations, among genetic groups or between treatments (range: 11.5−16.9 km d−1). However, individual variation in progression rates and migratory routes resulted in large differences in fjord residence times (range: 2−39 d). Ocean-current directions during and after release affected swimming speed, progression rate and route choice, and for most post-smolts, swimming speeds were much higher than their progression rates out of the fjord. The predicted lice loads based on lice intensity growth rates on smolts held in sentinel cages throughout the fjord indicated that individuals taking >10 d to exit the fjord in periods with high infestation pressure are likely to get lethally high sea-lice infestations. We conclude that, as migratory routes of S. salar post-smolts are hard to predict and migration times can stretch up to over a month, it is important to develop aquaculture management that keeps salmon lice levels down along all potential migration routes and during the full potential migratory period. Migratory behaviour · Salmo salar · Lepeophtheirus salmonis · Acoustic telemetry · Management · Fish farming · ParasitepublishedVersio

Prediction of long-term remission in patients following discontinuation of anti-TNF therapy in ulcerative colitis: a 10 year follow up study

Background - The long-term outcomes of Ulcerative colitis (UC) after discontinuation of biological therapy are largely unknown. There is also a lack of accurate and validated markers that can predict outcome after withdrawal accurately. The aims of this study were to describe the long-term outcomes in UC patients following cessation of anti-TNF therapy and explore potential biomarkers as an approach towards precision medicine. Methods - Seventy-five patients with moderate to severe UC treated to remission with anti-tumor necrosis factor (TNF) were included in the study. This is a follow-up of previously reported UC outcomes. The patients were categorized as either “Remission” or “Relapse”. The “Relapse” group was divided into subgroups determined by the highest treatment level needed to obtain remission the last 3 years of observation: non-biological therapy, biological therapy or colectomy. Remission were divided in long term remission (LTR), those using immunomodulating drugs (LTR + imids) and those using only 5-amino-salicylate (5-ASA) treatment (LTR) for the past 3 years. Analyses of mucosal gene expression by real-time PCR were performed. Results - The median (IQR) observation time of all patients included was 121 (111–137) months. Of the 75 patients, 46 (61%) did not receive biological therapy, including 23 (31%) in LTR ± imids. Of these 23 patients, 16 (21%) were defined as LTR with a median observation time of (IQR) 95 (77–113) months. In total 14 patients (19%) underwent colectomy during the 10 years after first remission. Mucosal TNF copies/µg mRNA  Conclusion - In this 10-year follow-up of UC of patients with moderate to severe disease, 61% of patients experience an altered phenotype to a milder disease course without need of biological therapy. Twenty-one percent of the patients were LTR without any medication except of 5-ASA. Mucosal TNF gene expression and IL1RL1- transcripts may be of clinical utility for long term prognosis in development of precision medicine in UC

Surgery with disc prosthesis versus rehabilitation in patients with low back pain and degenerative disc: two year follow-up of randomised study

Objective To compare the efficacy of surgery with disc prosthesis versus non-surgical treatment for patients with chronic low back pain

Kapasitetsutvikling og konkurranseforhold – Utvalgte grupper i kystflåten

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Diel vertical migration of Arctic zooplankton during the polar night

High-latitude environments show extreme seasonal variation in physical and biological variables. The classic paradigm of Arctic marine ecosystems holds that most biological processes slow down or cease during the polar night. One key process that is generally assumed to cease during winter is diel vertical migration (DVM) of zooplankton. DVM constitutes the largest synchronized movement of biomass on the planet, and is of paramount importance for marine ecosystem function and carbon cycling. Here we present acoustic data that demonstrate a synchronized DVM behaviour of zooplankton that continues throughout the Arctic winter, in both open and ice-covered waters. We argue that even during the polar night, DVM is regulated by diel variations in solar and lunar illumination, which are at intensities far below the threshold of human perception. We also demonstrate that winter DVM is stronger in open waters compared with ice-covered waters. This suggests that the biologically mediated vertical flux of carbon will increase if there is a continued retreat of the Arctic winter sea ice cover

Time series covering up to four decades reveals major changes and drivers of marine growth and proportion of repeat spawners in an Atlantic salmon population

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