Eddy Impacts on the Florida Current

The Gulf Stream in the Atlantic carries warm water northwards and forms both the return closure of the subtropical gyre as well as the upper limb of the meridional overturning circulation. Recent time series recorded east of the Bahamas at 26°N indicate that from May 2009 to April 2011, in contrast with past observations, the northward flowing Antilles Current covaried with the Gulf Stream in the Florida Straits—the Florida Current—even though the Florida and Antilles Currents are separated by banks and islands spanning 150?km. The peak-to-trough amplitude of transport variations during this period was 15?×?106?m3?s?1 for the Florida Current and 12?×?106?m3?s?1 for the Antilles Current, at time scales of 50?days to a year. From satellite observations, we show that the fluctuations in both the Florida and Antilles Currents between May 2009 and April 2011 are driven by eddy activity east of the Bahamas. Since the Florida Current time series is a critical time series for the state of the oceans, and often compared to climate models, this newly identified source of variability needs careful consideration when attributing the variability of the Florida Current to changes in the larger-scale circulations (e.g., gyre and overturning) or wind forcing.<br/

Natural nitrogen-15 abundance of soil and plant samples

Includes bibliographical references (page 50).Nitrogen isotope analysis of total soil N and soil-derived nitrate for nine selected Chernozemic and Luvisolic Ap horizons showed mean δa15N values based on atmospheric abundance of 8.8. Luvisolic soils were characterized by a relatively low level of the heavier isotope. Isotope enrichment of the total N reached a maximum in the lower B horizon. Subsoil nitrate (180-cm depth) had a δa15N value 1/3 that of the Ap horizon. The δa15N of subsurface soil horizons containing residual fertilizer N were low (−5.2) compared to the surface horizon (7.0). The δa15N of NH4-N in commercial fertilizers is close to that of atmospheric-N whereas the NO3-N has higher values. The data suggest that variations in δ15N abundance between horizons of the same soil, or between different soils, may be of real use in evaluating stresses which have been placed in the nitrogen cycle due to man's activities in the past. Similarly, differences in 15N abundance of soil, legume and air samples may provide an integrated estimation of symbiotic nitrogen fixation under field conditions. More detailed understanding of biological and other processes which control the N isotope concentrations must be obtained before the data reported can be further interpreted

Identification of a Defensin from the Hemolymph of the American Dog Tick, Dermacentor variabilis

Hemolymph from partially fed virgin Dermacentor variabilis females was collected following Borrelia burgdorferi challenge and assayed for antimicrobial activity against Bacillus subtilis and B. burgdorferi. A small inducible cationic peptide was identified by SDS-PAGE in the hemolymph of these ticks as early as 1h post challenge. Following purification by a three-step procedure involving sequential SepPak elution, reversed phase high performance liquid chromatography (RP-HPLC) and gel electrophoresis, the yield of the active peptide was approximately 0.1% of the total protein in the hemolymph plasma. The molecular weight, 4.2kDa, was determined by MALDI-TOF mass spectrometry. N-terminal sequencing by the Edman degradation method gave a sequence for the first 30 amino acids as: G-F-G-C-P-L-N-Q-G-A-C-H-N-H-C-R-S-I-(R)-(R)-(R)-G-G-Y-C-S-Q-I-I-K. A computer search of databases showed that the peptide had 83% similarity to a defensin found in a scorpion. This is the first report of a defensin from a tick. The peptide was stable at least up to 70 degree C. Although the tick defensin alone was not immediately effective against B. burgdorferi, tick defensin plus lysozyme killed more than 65% of cultured B. burgdorferi within 1h

Response of the Tick Dermacentor variabilis (Acari: Ixodidae) to Hemocoelic Inoculation of Borrelia burgdorferi (Spirochetales)

When Borrelia burgdorferi B31 low passage strain spirochetes were directly injected into the hemocoel of Dermacentor variabilis(Say) females, the bacteria were cleared from the hemocoel within \u3c 24 h. Viable spirochetes were not found in hemolymph, salivary gland, or ovary tissues by subculturing or by IFA. The hemocyte population increased ≈6 times within the first 6 h after inoculation compared with the uninoculated controls. In contrast, the soluble total hemolymph protein content decreased inversely with the increase in hemocytes. Borreliacidal activity was demonstrated with cell-free hemolymph from D. variabilis. In vitro antimicrobial assays using hemolymph from borrelia-challenged and nonchallenged ticks resulted in 72% spirochete reductions compared with only 11.5%, respectively, within 1 h. Additional evidence of induced antimicrobial hemolymph protein activity was demonstrated by SDS-PAGE, which revealed upregulation of a lysozyme-like peptide (≈ 15 kDa) (22% increase) and the induction of a ≈ 5.8 kDa peptide in the B. burgdorferi-challenged ticks. In contrast with the nonvector borne Bacillus subtilis, D. variabilis presented a rapid and robust response to challenge with cultured B. burgdorferi spirochetes and lead to their early elimination. The role of the tick immune system, including possible differences between vector and nonvector ticks, in determining the success of invasive bacteria is discussed

Accretion-powered Stellar Winds as a Solution to the Stellar Angular Momentum Problem

We compare the angular momentum extracted by a wind from a pre-main-sequence star to the torques arising from the interaction between the star and its Keplerian accretion disk. We find that the wind alone can counteract the spin-up torque from mass accretion, solving the mystery of why accreting pre-main-sequence stars are observed to spin at less than 10% of break-up speed, provided that the mass outflow rate in the stellar winds is ~10% of the accretion rate. We suggest that such massive winds will be driven by some fraction $\epsilon$ of the accretion power. For observationally constrained typical parameters of classical T-Tauri stars, $\epsilon$ needs to be between a few and a few tens of percent. In this scenario, efficient braking of the star will terminate simultaneously with accretion, as is usually assumed to explain the rotation velocities of stars in young clusters.Comment: Accepted by ApJ Letter

The Far-Ultraviolet Spectra of TW Hya. II. Models of H2 Fluorescence in a Disk

We measure the temperature of warm gas at planet-forming radii in the disk around the classical T Tauri star (CTTS) TW Hya by modelling the H2 fluorescence observed in HST/STIS and FUSE spectra. Strong Ly-alpha emission irradiates a warm disk surface within 2 AU of the central star and pumps certain excited levels of H2. We simulate a 1D plane-parallel atmosphere to estimate fluxes for the 140 observed H2 emission lines and to reconstruct the Ly-alpha emission profile incident upon the warm H2. The excitation of H2 can be determined from relative line strengths by measuring self-absorption in lines with low-energy lower levels, or by reconstructing the Ly-alpha profile incident upon the warm H2 using the total flux from a single upper level and the opacity in the pumping transition. Based on those diagnostics, we estimate that the warm disk surface has a column density of log N(H2)=18.5^{+1.2}_{-0.8}, a temperature T=2500^{+700}_{-500} K, and a filling factor of H2, as seen by the source of Ly-alpha emission, of 0.25\pm0.08 (all 2-sigma error bars). TW Hya produces approximately 10^{-3} L_\odot in the FUV, about 85% of which is in the Ly-alpha emission line. From the H I absorption observed in the Ly-alpha emission, we infer that dust extinction in our line of sight to TW Hya is negligible.Comment: Accepted by ApJ. 26 pages, 17 figures, 6 table

A rapid, chromatography-free route to substituted acridine–isoalloxazine conjugates under microwave irradiation

Microwave irradiation was applied to a sequence of condensation reactions from readily available 9-chloroacridines to provide a range of novel acridine–isoalloxazine conjugates. The combination of these two moieties, both of biological interest, was achieved by a chromatography free route

A prototype system for observing the Atlantic Meridional Overturning Circulation - scientific basis, measurement and risk mitigation strategies, and first results

The Atlantic Meridional Overturning Circulation (MOC) carries up to one quarter of the global northward heat transport in the Subtropical North Atlantic. A system monitoring the strength of the MOC volume transport has been operating since April 2004. The core of this system is an array of moored sensors measuring density, bottom pressure and ocean currents. A strategy to mitigate risks of possible partial failures of the array is presented, relying on backup and complementary measurements. The MOC is decomposed into five components, making use of the continuous moored observations, and of cable measurements across the Straits of Florida, and wind stress data. The components compensate for each other, indicating that the system is working reliably. The year-long average strength of the MOC is 18.7±5.6 Sv, with wind-driven and density-inferred transports contributing equally to the variability. Numerical simulations suggest that the surprisingly fast density changes at the western boundary are partially linked to westward propagating planetary wave

Applicability of ERTS-1 to lineament and photogeologic mapping in Montana: Preliminary report

A lineament map prepared from a mosaic of western Montana shows about 85 lines not represented on the state geologic map, including elements of a northeast-trending set through central western Montana which merit ground truth checking and consideration in regional structural analysis. Experimental fold annotation resulted in a significant local correction to the state geologic map. Photogeologic mapping studies produced only limited success in identification of rock types, but they did result in the precise delineation of a late Cretaceous or early Tertiary volcanic field (Adel Mountain field) and the mapping of a connection between two granitic bodies shown on the state map. Imagery was used successfully to map clay pans associated with bentonite beds in gently dipping Bearpaw Shale. It is already apparent that ERTS imagery should be used to facilitate preparation of a much needed statewide tectonic map and that satellite imagery mapping, aided by ground calibration, provides and economical means to discover and correct errors in the state geologic map