Construction of a knockout targeting vector to generate an Interleukin-13 Receptor α1 deficient Balb/c mouse

Abstract only availableA recent publication from our lab has provided evidence for the involvement of the α1 chain of the Interleukin-13 cytokine receptor (IL-13Rα1) in the development of the neonatal immune system. Specifically, we have shown that cell death of T helper type 1 (Th1) effector cells can be prevented by antibody-mediated blockade of IL-13Rα1. Currently, a knockout mouse deficient in expression of IL-13Rα1 is not available and the development of an IL-13Rα1 knockout mouse will provide new insights on the relationship between IL-13Rα1 signaling and neonatal immunity. In this effort we have begun construction of a targeting vector that will bear sufficient homology to the IL-13Rα1 wild-type locus to allow for deletion of exons 7, 8, and 9 via homologous recombination, thereby rendering that allele non-functional. After construction of the targeting vector, a collaborative effort between the Transgenic Animal Core facility here at the University of Missouri will continue throughout the remainder of the cell culture, embryonic manipulation, and screening processes.Life Sciences Fellowships/Meyerhoff Scholars Progra

A study of the antinociceptive interaction between intrathecal opioids and α-adrenergic agonists in the spinal cord of the rat

The role and mechanism of action of α₁-adrenoceptors in adrenergic spinal antinociception is uncertain. α₁-Adrenoceptors are normally excitatory in the CNS, suggesting that any inhibitory effect on nociception must be indirect. Recently, we showed that intrathecal (i.t.) α₁-agonists potentiate α₂-induced spinal antinociception in the rat by a δ-receptor mediated, enkephalin-dependent process. If enkephalins, released from spinal interneurons, mediate the antinociceptive effect of α₁-adrenoceptors, then non-selective α-agonists (i.e. norepinephrine; NE) should exhibit cross-tolerance to δ-agonists (i.e. DADLE), but not to μ-agonists (i.e. morphine), in the spinal cord. Conversely, the antinociceptive effect of α₂-selective agonists (i.e, dexmedetomidine; DX) should be unaffected by spinal opioid tolerance. To test these hypotheses, male, Sprague-Dawley rats (300-400g) were continuously infused with i.t. saline (1 μl/h), morphine (MOR; 5, 10, or 20 μg/h) or DADLE (10 μg/h) for 6 days using ALZET osmotic mini-pumps. Antinociception was assessed using the tail flick (TF) test. In an initial time course study, significant recovery from DADLE and MOR tolerance did not occur until day 3 and 4 post-infusion (PI), respectively. In subsequent cross-tolerance experiments, NE and DX dose-response curves were determined on days 1 and 2 PI for DADLE-pretreated rats, and on days 1-3 PI in MOR-pretreated rats. The table of ED₅₀ ratios for i.t. NE and DX in opioid- and saline-infused animals demonstrates that NE exhibits significant (*) cross-tolerance to DADLE, but not morphine. No cross-tolerance was observed between DX and DADLE. -- [table in abstract is omitted] -- In separate groups of rats, i.p. naloxone significantly attenuated the antinociceptive effect of MOR, but not DADLE, on day 1 of infusion (time of peak antinociception); i.p. naltrindole significantly antagonized DADLE, but not MOR. These data indicate that μ- and δ-receptor selectivity was retained during infusion, consistent with the different cross-tolerance results to NE. The marked antagonism of i.t. DX by Wyeth 27127, but not by prazosin, confirmed the α₂-selectivity of DX at the doses used. The results of this study are consistent with the hypothesis that α₁-adrenoceptors facilitate the release of enkephalins in the spinal cord which, in turn, effect antinociception by a δ-receptor mechanism

Identificación y diagnóstico para la implementación de un programa para la conservación auditiva en las centrales de generación en la empresa Emgesa S.A. E.S.P.

73p.In the implementation of this Program for Hearing Conservation, developed systematic actions for environmental assessment of noise, recommendation e implementation of control measures, biological monitoring in workers, selection of personal hearing protection elements, Training and verification of the results of the Program. NIOSH recommends that hearing conservation programs be implemented for all unprotected workers who are exposed for 8 hours a day working at intensities equal to or greater than 85 decibels (A) and that the program include at least the following components. 1. Initial and annual Review Procedures. 2. Analysis of noise exposure. 3. Administrative technical controls of noise exposure. 4. Audiometric evaluation and monitoring of workers' hearing 5. Auditory prevention in workers. 6. Education and Motivation. 7. Keep a record. 8. Evaluation of the Program's Effectiveness.En la implementación del presente Programa para la Conservación Auditiva ha desarrollado acciones sistemáticas de evaluación ambiental del ruido, recomendación e implementación de medidas de control, monitoreo biológico en los trabajadores, selección de elementos de protección personal auditiva, Capacitación y verificación de los resultados del Programa. NIOSH recomienda que los programas de conservación auditiva sean implementados para todos los trabajadores que no estén protegidos y se exponen durante 8 horas diarias de trabajo a intensidades iguales o mayores de 85 decibeles (A) y que el programa incluya por lo menos los siguientes componentes. 1. Procedimientos de Revisión Inicial y anual. 2. Análisis de exposición al ruido. 3. Controles técnicos administrativos de exposición al Ruido. 4. Evaluación Audiométrica y monitoreo de la audición de los trabajadores 5. Prevención auditiva en los trabajadores. 6. Educación y Motivación. 7. Mantener un registro. 8. Evaluación de la Efectividad del Programa

Estimación, aceptación y opinión de la brecha de recursos humanos asistenciales en el Hospital Regional de Ayacucho, “Miguel Àngel Mariscal Llerena”, para la categoría III-1, 2018

El presente trabajo de investigacion tiene como objetivo general de: Establecer la relación del nivel de conocimiento y la aceptación de la estimación de la brecha recursos humanos asistenciales del Hospital Regional de Ayacucho “Miguel Àngel Mariscal Llerena”. Investigación de enfoque Mixto (Cuantitativo: Descriptivo, explicativo, relacional) (cualitativo: Fenomenológico), multivariable. En una muestra de datos de 744 servidores públicos asistenciales, 10 jefes de departamento, 01 funcionario del HRA, 01 funcionario de la Dirección Regional de Salud de Ayacucho y 01 del Ministerio de Salud. La técnica empleada fue, análisis documental, fichaje, encuetas a jefes de departamentos y entrevista a funcionarios, los instrumentos empleados fueron: Fichas de análisis documental, cuestionario y guía de entrevista, sometido a una prueba de confiabilidad de Alfa de Cronbach (0.802) Teniendo como resultados: La brecha actual es de 822 RHUS asistenciales del HRA para la categoría III-1. El nivel de conocimiento es bueno, con una aceptación alta de los jefes de departamento sobre estimación de la brecha de RHUS asistenciales del HRA. Existe una relación moderada y directamente proporcional entre el nivel de conocimiento y la aceptación de la estimación de la brecha de RHUS asistenciales en los jefes de departamento del HRA (rtb = 0,646; p = 0,013). Opinaron los jefes de departamento estar de acuerdo con la metodología de estimación y que su implementación sea de acuerdo a la necesidad institucional y de cada servicios. Los funcionarios opinaron que la metodología es una propuesta importante del MINSA validada, pero se realizó desde la oferta por tener una infraestructura y equipamiento nueva, existe deficiencias en el expediente técnico de la nueva infraestructura. Siendo su implementación de la brecha por el MINSA, muy crítica por déficit de médicos especialistas a nivel regional y nacional; por falta de decisiones políticas y técnicas. Sugeriendo a las autoridades regionales de turno que con los resultados de esta investigación realicen gestiones a nivel regional y nacional como en el Ministerio de Salud, Ministerio de Economía y Finanzas y la Presidencia del Consejo de Ministros

A new construction of anticode-optimal Grassmannian codes

In this paper, we consider the well-known unital embedding from \FF_{q^k} into M_k(\FF_q) seen as a map of vector spaces over \FF_q and apply this map in a linear block code of rate $\rho/\ell$ over \FF_{q^k}. This natural extension gives rise to a rank-metric code with $k$ rows, $k\ell$ columns, dimension $\rho$ and minimum distance $k$ that satisfies the Singleton bound. Given a specific skeleton code, this rank-metric code can be seen as a Ferrers diagram rank-metric code by appending zeros on the left side so that it has length $n-k$. The generalized lift of this Ferrers diagram rank-metric code is a Grassmannian code. By taking the union of a family of the generalized lift of Ferrers diagram rank-metric codes, a Grassmannian code with length $n$, cardinality $\frac{q^n-1}{q^k-1}$, minimum injection distance $k$ and dimension $k$ that satisfies the anticode upper bound can be constructed

NLTE model calculations for the solar atmosphere with an iterative treatment of opacity distribution functions

Modeling the variability of the solar spectral irradiance is a key factor for understanding the solar influence on the climate of the Earth. As a first step to calculating the solar spectral irradiance variations we reproduce the solar spectrum for the quiet Sun over a broad wavelength range with an emphasis on the UV. We introduce the radiative transfer code COSI which calculates solar synthetic spectra under conditions of non-local thermodynamic equilibrium (NLTE). A self-consistent simultaneous solution of the radiative transfer and the statistical equation for the level populations guarantees that the correct physics is considered for wavelength regions where the assumption of local thermodynamic equilibrium (LTE) breaks down. The new concept of iterated opacity distribution functions (NLTE-ODFs), through which all line opacities are included in the NLTE radiative transfer calculation, is presented. We show that it is essential to include the line opacities in the radiative transfer to reproduce the solar spectrum in the UV. Through the implemented scheme of NLTE-ODFs the COSI code is successful in reproducing the spectral energy distribution of the quiet Sun.Comment: 13 pages, 9 figures. accepted for publication in Astronomy and Astrophysic

El Control Judicial de los Preacuerdos

El presente trabajo aborda el control judicial de los preacuerdos que realiza el juez de conocimiento, el cual se compone de un primer control de los aspectos de carácter formal (Const.1991, Art. 131) y excepcionalmente uno de carácter material, cuando de manera inapropiada y arbitraria se desconozcan o quebranten las garantías fundamentales de las partes e intervinientes, caso en el cual es deber del juzgador intervenir en procura de salvaguardar estas garantías de orden constitucional y legal.Universidad Libre - Facultad de Derecho - Especialización en Derecho Penal y CriminologíaThis paper addresses the issue of judicial control of pre-agreements made by the judge of knowledge, which consists of a first control of the formal aspects (Const.1991, Art. 131) and exceptionally a material nature, when in a way rude and arbitrary ignore or violate the fundamental guarantees of the parties and intervening, in which case it is the duty of the judge to intervene in order to safeguard these constitutional and legal guarantees