INPPO actions and recognition as a driving force for progress in plant proteomics: Change of guard, INPPO update, and upcoming activities

The International Plant Proteomics Organization (INPPO) is a non-profit organization whose members are scientists involved or interested in plant proteomics. Since the publication of the first INPPO highlights in 2012, continued progress on many of the organization’s mandates/goals has been achieved. Two major events are emphasized in this second INPPO highlights. First, the change of guard at the top, passing of the baton from Dominique Job, INPPO founding President to Ganesh Kumar Agrawal as the incoming President. Ganesh K. Agrawal, along with Dominique Job and Randeep Rakwal initiated the INPPO. Second, the most recent INPPO achievements and future targets, mainly the organization of first the INPPO World Congress in 2014, tentatively planned for Hamburg (Germany), are mentioned.Web of Scienc

: Seizure onset zone imaging

International audienceStereo-electroencephalography is used to localize the seizure onset zone and connected neuronal networks in surgical candidates suffering from intractable focal epilepsy. The concept of an epileptogenicity index has been proposed recently to represent the likelihood of various regions being part of the seizure onset zone. It quantifies low-voltage fast activity, the electrophysiological signature of seizure onset usually assessed visually by neurologists. Here, we revisit epileptogenicity in light of neuroimaging tools such as those provided in statistical parametric mapping software. Our goal is to propose a robust approach, allowing easy exploration of patients' brains in time and space. The procedure is based upon statistical parametric mapping, which is an established framework for comparing multi-dimensional image data that allows one to correct for inherent multiple comparisons. Statistics can also be performed at the group level, between seizures in the same patient or between patients suffering from the same type of epilepsy using normalization of brains to a common anatomic atlas. Results are obtained from three case studies (insular reflex epilepsy, cryptogenic frontal epilepsy and lesional occipital epilepsy) where tailored resection was performed, and from a group of 10 patients suffering from mesial temporal lobe epilepsy. They illustrate the basics of the technique and demonstrate its very good reproducibility and specificity. Most importantly, the proposed approach to the quantification of the seizure onset zone allows one to summarize complex signals in terms of a time-series of statistical parametric maps that can support clinical decisions. Quantitative neuroimaging of stereo-electroencephalographic features of seizures might thus help to provide better pre-surgical assessment of patients undergoing resective surgery

Lay perceptions of breast cancer in Western Kenya

AIM: To explore lay perceptions of causes, severity, presenting symptoms and treatment of breast cancer. METHODS: In October-November 2012, we recruited men and women (18 years and older) from households and health facilities in three different parts of Western Kenya, chosen for variations in their documented burdens of breast cancer. A standardized and validated tool, the breast cancer awareness measure (BCAM), was administered in face-to-face interviews. Survey domains covered included socio-demographics, opinions about causes, symptoms, severity, and treatment of breast cancer. Descriptive analyses were done on quantitative data while open-ended answers were coded, and emerging themes were integrated into larger categories in a qualitative analysis. The open-ended questions had been added to the standard BCAM for the purposes of learning as much as the investigators could about underlying lay beliefs and perceptions. RESULTS: Most respondents were female, middle-aged (mean age 36.9 years), married, and poorly educated. Misconceptions and lack of knowledge about causes of breast cancer were reported. The following (in order of higher to lower prevalence) were cited as potential causes of the condition: Genetic factors or heredity (n = 193, 12.3%); types of food consumed (n = 187, 11.9%); witchcraft and curses (n = 108, 6.9%); some family planning methods (n = 56, 3.6%); and use of alcohol and tobacco (n = 46, 2.9%). When asked what they thought of breast cancer’s severity, the most popular response was “it is a killer disease” (n = 266, 19.7%) a lethal condition about which little or nothing can be done. While opinions about presenting symptoms and signs of breast cancer were able to be elicited, such as an increase in breast size and painful breasts, early-stage symptoms and signs were not widely recognized. Some respondents (14%) were ignorant of available treatment altogether while others felt breast cancer treatment is both dangerous and expensive. A minority reported alternative medicine as providing relief to patients. CONCLUSION: The impoverished knowledge in these surveys suggests that lay education as well as better screening and treatment should be part of breast cancer control in Kenya

Biogenic Emission Measurements from Birch and Spruce Trees using Vocus PTR-TOF

Biogenic Volatile Organic Compounds play a major role in the atmosphere by acting as precursors in the formation of secondary organic aerosols and by also affecting the concentration of ozone. The chemical diversity of BVOCs is vast but global emissions are dominated by isoprene and monoterpenes. The emissions of BVOCs from plants are affected by environmental parameters with temperature and light having significant impacts on the emissions. The Downy birch and Norway spruce trees consist of heavy and low volatile compounds but published results are limited up to observing sesquiterpenoid emissions from these two trees. In this study, the Vocus proton-transfer-reaction time-of-flight mass spectrometer is deployed in the field to examine BVOC emissions from Downy birch and Norway spruce trees. With higher mass resolution, shorter time response and lower limits of detection than conventional PTR instruments, the Vocus can effectively measure a broader range of VOCs. For the first time, real-time emissions of diterpenes and 12 different oxygenated compounds were observed from birch and spruce trees. The emission spectrum of birch was dominated by C10H17+, while for spruce C5H9+ contributed the most. The sum emissions of oxygenated compounds contributed significantly to the observed total emissions from both the trees. The emission rates of all compounds varied dramatically throughout the period due to fluctuations in temperature and light. Due to lack of data from spruce, conclusive results for temperature and light response on terpene emissions could not be drawn. For birch, the emission rates were well explained by the temperature and temperature-light algorithms. The terpene emissions modelled using both algorithms correlated similarly with experimental data making it difficult to decisively conclude if the emissions originated from synthesis or pools

Numerical analysis of cement panels reinforced with galvanized iron or polypropylene meshes

This paper deals with the numerical analysis of panels reinforced with galvanized iron (GI) or polypropylene (PP) meshes. It has been a common practice to use galvanized iron meshes as reinforcement in panels and is popularly called as ferrocement panels. Elevated humidity level and presence of salts can lead to corrosion of these galvanized iron meshes, leading to reduced service life. A rust-freepolypropylene mesh can be used as an alternate to the steel mesh. This paper presents numerical analysis in ANSYS Workbench based on an experimental study published in the literature” Comparative study of ferrocement panels reinforced with galvanized iron and polypropylene meshes”. A total of 16 rectangular panels tested in flexure was analyzed. Out of 16 panels, 8 were reinforced with GI mesh and 8 with PP mesh. The specimens were simply supported on two short edges and subjected to four-point bending. The parameters investigated include thickness of panels, volume fraction and the material of the mesh. All the GI mesh panels showed better strength than the corresponding PP mesh reinforcedcement panels. However, PP mesh panels exhibited better ductility as compared to GI mesh panels. An increase of 54% is noticed in 40mm thick panels reinforced with GI mesh, when compared to 20mm thick panels. Similarly, an increase of 83% is noticed in 40mm thick panels reinforced with PP mesh, when compared to 20mm thick panels. The results of numerical analysis were found to be comparable with the corresponding experimental results

The impact of substance use on brain structure in people at high risk of developing schizophrenia

Ventricular enlargement and reduced prefrontal volume are consistent findings in schizophrenia. Both are present in first episode subjects and may be detectable before the onset of clinical disorder. Substance misuse is more common in people with schizophrenia and is associated with similar brain abnormalities. We employ a prospective cohort study with nested case control comparison design to investigate the association between substance misuse, brain abnormality, and subsequent schizophrenia. Substance misuse history, imaging data, and clinical information were collected on 147 subjects at high risk of schizophrenia and 36 controls. Regions exhibiting a significant relationship between level of use of alcohol, cannabis or tobacco, and structure volume were identified. Multivariate regression then elucidated the relationship between level of substance use and structure volumes while accounting for correlations between these variables and correcting for potential confounders. Finally, we established whether substance misuse was associated with later risk of schizophrenia. Increased ventricular volume was associated with alcohol and cannabis use in a dose-dependent manner. Alcohol consumption was associated with reduced frontal lobe volume. Multiple regression analyses found both alcohol and cannabis were significant predictors of these abnormalities when simultaneously entered into the statistical model. Alcohol and cannabis misuse were associated with an increased subsequent risk of schizophrenia. We provide prospective evidence that use of cannabis or alcohol by people at high genetic risk of schizophrenia is associated with brain abnormalities and later risk of psychosis. A family history of schizophrenia may render the brain particularly sensitive to the risk-modifying effects of these substances

A self-assembled luminescent host that selectively senses ATP in water.

Metal-ion-directed self-assembly has been used to construct kinetically inert, water-soluble heterometallic Ru2Re2 hosts that are potential sensors for bioanions. A previously reported metallomacrocycle and a new derivative synthesised by this approach are found to be general sensors for bioanions in water, showing an “off–on” luminescent change that is selective for nucleotides over uncharged nucleobases. Through a change in the ancillary ligands coordinated to the ruthenium centres of the host, an “off–on” sensor has been produced. Whilst this host only shows a modest enhancement in binding affinities for nucleotides relative to the other two host systems, its sensing response is much more specific. Although a distinctive “off–on” luminescence response is observed for the addition of adenosine triphosphosphate (ATP), related structures such as adenine and guanosine triphosphate (GTP) do not induce any emission change in the host. Detailed and demanding DFT studies on the ATP- and GTP-bound host–guest complexes reveal subtle differences in their geometries that modulate the stacking interactions between the nucleotide guests and the ancillary ligands of the host. It is suggested that this change in stacking geometries affects solvent accessibility to the binding pocket of the host and thus leads to observed difference in the host luminescence response to the guests

SWAPO’s 50/50 Policy in Namibia’s National Assembly (2015-2018): Full of Sound and Fury Signifying Nothing?

In 2013, Namibia’s ruling party, the South West Africa People’s Organisation (SWAPO), amended its constitution to implement the 50/50 gender policy. This policy required equal representation of men and women in all its leadership structures and in its representation in institutions such as the National Assembly. The party had been zigzagging on this policy it had adopted at its 2002 congress. Four years since the implementation of SWAPO’s 50/50, an analysis of the impact of this policy in the National Assembly paints a troubling picture. At the level of substance by looking at parliamentary motions tabled, we find that SWAPO’s 50/50 policy in the National Assembly – which resulted in increased number of women in the legislature - did not lead to meaningful agenda setting in favour of women political participation. This article reflects critically on the gender policy in the 6th parliament’s National Assembly, which is the principal law-making and policy-setting arm of parliament with a view to assess whether there have been successes in facilitating women empowerment and participation in a meaningful way

Challenging Ableist Norms: Old Age, Disability, and its Impact on Family Dynamics in Rohinton Mistry\u27s Family Matters

This paper explores the intersection of old age, disability, and family dynamics in Rohinton Mistry\u27s Family Matters through the lens of social constructionism. It subverts existing ableist norms that are nurtured through a conventional social lens. This study examines the effects of societal perceptions on disability and aging. These, in turn, affect familial relationships and individual identities. The analysis is fortified through the social constructionism theory of Peter Berger and Thomas Luckmann. This article delves into textual analysis and discusses subjectivity and objectivity with reference to the agonizing experiences of elderly people with disabilities within their families, the detrimental effects of ableism on mental and physical well-being, issues with family identity, and the complex and wide-ranging effects of discrimination against families with members who have disabilities. It affords a space to deliberate on existing societal perceptions of normalcy and functionality in contrast to the perspective posited by Berger and Luckman. The paper posits how \u27social stock of knowledge\u27 and \u27zones of familiarity’ influence the experiences of Nariman Vakeel, an elderly patriarch suffering from Parkinson\u27s disease, whose physical decline forces his family to confront deep-rooted prejudices and expectations surrounding care, dependency, and the value of life in old age. By applying social constructionism, the analysis delves into how disability is not merely a biological condition but is shaped by cultural, social, and familial responses. The novel exposes the tension between the societal expectation of independence and the reality of vulnerability and how these constructs influence the treatment of the elderly and disabled